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OfflineJahLambsbread
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incubating chamber got to 105 degrees F by accident
    #18803109 - 09/05/13 12:08 PM (10 years, 4 months ago)

Two jars were in a chamber colonizing.

There was a heater under the chamber because it is getting colder outside.

The temperature accidentally went to 107 degrees Farenheit.

Will this kill anything in the jars?  They felt really warm


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InvisibleFrankHorrigan
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Re: incubating chamber got to 105 degrees F by accident [Re: JahLambsbread]
    #18803123 - 09/05/13 12:11 PM (10 years, 4 months ago)

Most likely they will be damaged but they may recover.

Use my tub-in-tub incubator, much more precise :thumbup:


--------------------

Yes, you can bump my old threads with a question.
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You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Offlineghostinthemist
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18803184 - 09/05/13 12:30 PM (10 years, 4 months ago)

high temps like that also allow for contamination to take over.  Watch for slimmy greasy looking spots or odd smells coming from them.  Also, if your temps aren't getting below 55 a incubator is not needed.  i currently have 2 monotubs 2 sgfc and 50 half pints in a room that has a window constantly open for fresh air and the nights have been getting to 56 without any growth issues.  Along with the fact that myce will produce there own heat \.  As much as 10+outside temp


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InvisibleFrankHorrigan
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Re: incubating chamber got to 105 degrees F by accident [Re: ghostinthemist]
    #18803197 - 09/05/13 12:35 PM (10 years, 4 months ago)

Quote:

ghostinthemist said:
high temps like that also allow for contamination to take over.




No, this is a school of thought that rides the short bus.

There should be no contamination present in your jars. If there is contamination "in-waiting" then your jar is already fucked. There is no "winning" against contams.

And if you are talking endospores...endospores are not going to be stimulated even at 86F.

I know this from the many thousands of jars I've placed in my incubator. They are fine at 80F. They are even fine at 86F.

Quote:

Also, if your temps aren't getting below 55 a incubator is not needed.




Spoken like someone who has never witnessed the benefits of consistent temps 24 hours a day. You should try it :wink:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Offlineghostinthemist
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18803282 - 09/05/13 01:00 PM (10 years, 4 months ago)

Been there done it.  I guess technically my grow room is a incubator or any space that maintains a constant temperature that allows for optimum growth.  Mine 78 during day 50's at night 100% after 8 days.  But these are all opinions.  RR states no incubator should ever be needed.  Here is some more regurgitated meaningless info

"Beginners are the biggest reason NOT to use an incubator.  Internal jar temps above 82F actually slow down mycelium growth, and also stimulate contaminant molds.  If your incubator is running 81F to 84F, your internal jar temps are going to be 85 to 90, considering that glass is an insulator and mycelium produces heat as it colonizes.  Nearly all the growers who used to use incubators have now stopped.  Those who haven't have probably never tried to colonize on an open shelf at normal room temp, or they'd have stopped too. 

Wait until your jars are nearly done and your heater sticks and you find your incubator over 100F with all your jars ruined.  That will make a believer out of you.

In addition, stale air in incubators are a major cause of contamination."

Spoken like someone that hasn't used the search engine
RR


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Offlineghostinthemist
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Re: incubating chamber got to 105 degrees F by accident [Re: ghostinthemist]
    #18803303 - 09/05/13 01:05 PM (10 years, 4 months ago)

you notice RR talking about contams.  That's because they are always present.  Either in the substrate itself or in the "filter layer"  there are even endospores and microbes that aren't killed by pc or steam just beat down by rapidly growing myce.  Please don't give out incorrect info.  It's a rule ya know.  I was banned for a week from "people stating i was misleading people and attempting to ruin there grows" but after having the mods review my info that i had given out they allowed me back in and just told me to report any hazing of any kind.  So lets keep this professional with quoted information like the rules state.  Not opinions and heresay


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InvisibleFrankHorrigan
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Re: incubating chamber got to 105 degrees F by accident [Re: ghostinthemist]
    #18803307 - 09/05/13 01:07 PM (10 years, 4 months ago)

You can quote shit at me all day man.  You're just a parrot, we all see that.

Thus your terrible rating.

OP, your best bet is to ignore all the advice of ghostinthemist and listen to me or just about anyone else.

PS. Hey ghost, I am a trusted cultivator. Your attempts to "educate" me fall on deaf ears. I'm aware of all the info you have ever presented, I have done my own series of tests over the years, and I present an unbiased opinion on what I see is the problem based on the results of my own tests, knowledge and the research of others :wink:

Name dropping RR just makes you look like a big baby who doesn't grow much but wishes he was someone.

Try out an incubator or quit talking about it :lol:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Edited by FrankHorrigan (09/05/13 01:14 PM)


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Offlineghostinthemist
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18803316 - 09/05/13 01:10 PM (10 years, 4 months ago)

im reporting you for trolling and hazing


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When all seems lost it has been neglected for too long to be found


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OfflinePussyFart
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Re: incubating chamber got to 105 degrees F by accident [Re: ghostinthemist]
    #18803319 - 09/05/13 01:11 PM (10 years, 4 months ago)

:curbyourenthusiasm:


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InvisibleFrankHorrigan
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Re: incubating chamber got to 105 degrees F by accident [Re: ghostinthemist]
    #18803321 - 09/05/13 01:11 PM (10 years, 4 months ago)

Quote:

ghostinthemist said:
im reporting you for trolling and hazing




:lmafo:

Go right ahead.

I'll be sure to let FooMan, EvilMushroom, Citric and all the other mods that I speak to regularly know that you should be banned again.

See, that's how you name-drop like a big boy :cool:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Offlineghostinthemist
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18803344 - 09/05/13 01:21 PM (10 years, 4 months ago)

Did and im done with it.  RR is a trusted cultivator you are not.  So it is better for you and i to use info that is available instead of dropping to a sophomoric level such as you have.  Back your info up with years and years of XP otherwise shut the fuck up and quit your crying.


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When all seems lost it has been neglected for too long to be found


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InvisibleFrankHorrigan
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Re: incubating chamber got to 105 degrees F by accident [Re: ghostinthemist]
    #18803352 - 09/05/13 01:23 PM (10 years, 4 months ago)

Actually dude, I am a trusted cultivator.

RR is a moderator on these boards.

Thus, my trusted cultivator tag, clearly visible below my avatar on the left, was given to me in part by RR.

So you can draw the conclusion easy enough, technically I have the RR stamp of approval. Not to mention the approval of the rest of the mods.

And you do not :lol:

Let's see which report they take more seriously, mine or yours.

:waitingpatiently:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Edited by FrankHorrigan (09/05/13 01:40 PM)


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Offlineghostinthemist
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18803369 - 09/05/13 01:28 PM (10 years, 4 months ago)

idk you keep shooting down RR info so your just a worthless southern fucking idiot.  But that's your moms fault for fucking her brother


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Offlineghostinthemist
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Re: incubating chamber got to 105 degrees F by accident [Re: ghostinthemist]
    #18803373 - 09/05/13 01:30 PM (10 years, 4 months ago)

Wait you little cousin fucker you put a note out on me for what???? Providing correct useful info and trying to help people FUCK YOURSELF!


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InvisibleFrankHorrigan
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Re: incubating chamber got to 105 degrees F by accident [Re: ghostinthemist]
    #18803374 - 09/05/13 01:30 PM (10 years, 4 months ago)

Here comes the ban :bananadance:


Quote:

FrankHorrigan said:
Actually dude, I am a trusted cultivator.

RR is a moderator on these boards.

Thus, my trusted cultivator tag, clearly visible below my avatar on the left, was given to me in part by RR.

So you can draw the conclusion easy enough, technically I have the RR stamp of approval. Not to mention the approval of the rest of the mods.




:whathesaid:

:bye:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Edited by FrankHorrigan (09/05/13 01:41 PM)


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Offlineghostinthemist
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18803376 - 09/05/13 01:31 PM (10 years, 4 months ago)

If you wrote anything else i don't care i ignored your ass


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When all seems lost it has been neglected for too long to be found


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InvisibleShroomismM
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Re: incubating chamber got to 105 degrees F by accident [Re: ghostinthemist]
    #18803394 - 09/05/13 01:37 PM (10 years, 4 months ago)

Enjoy your break. Next time it will be a perma. You've been warned about this behavior multiple times, I guess it doesn't sink in does it?


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InvisibleFrankHorrigan
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18803422 - 09/05/13 01:50 PM (10 years, 4 months ago)

Thanks Shroomism!

Jah, sorry your thread got derailed. Here is my advice based on what you originally posted:

Quote:

FrankHorrigan said:
Most likely they will be damaged but they may recover.

Use my tub-in-tub incubator, much more precise :thumbup:




--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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InvisibleShroomismM
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18803426 - 09/05/13 01:52 PM (10 years, 4 months ago)

Temps over 105 could definitely damage the mycelium, but if it was only a short time it should hopefully be able to recover. Tub in tub is a good way to go :thumbup:


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InvisibleSpitballJedi
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18804179 - 09/05/13 05:04 PM (10 years, 4 months ago)

Quote:

FrankHorrigan said:
Quote:

ghostinthemist said:
high temps like that also allow for contamination to take over.




No, this is a school of thought that rides the short bus.

There should be no contamination present in your jars. If there is contamination "in-waiting" then your jar is already fucked. There is no "winning" against contams.





I would like to discuss this a bit if I may.

In the past, my understanding has been that, although we say "sterilize", we are not really 100% sterilizing and the point of sterilizing is to give the culture of our choice the head-start; to take over the jar. And, higher temperatures benefit the unwanted spores without really benefiting our chosen culture.

In light of my own experiments, I'm not sure my thinking is true. It's not actually my thinking, I got it from somewhere.

I have left many jars of sterilized grain out on the kitchen counter for several weeks. I have left some jars for 2 months. None of them contaminated. It would seem that at least one would have grown something if it were not actually sterilized.

I have read a couple of posts by people saying what you are saying Frank. Is my old way of thinking just outdated? Was it not a common held belief at one time? Or did I just read something stupid a long time ago and it got stuck in my head?

***Edit*** While searching something else, I found this:

http://www.shroomery.org/forums/showflat.php/Number/17609064#17609064
Quote:

TranscendingLife said:
Not all bacterial endospores are killed during the PC process.
We're just giving the mushroom mycelium a better chance at growing vs. contams.




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Edited by SpitballJedi (09/05/13 07:58 PM)


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InvisibleFrankHorrigan
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Re: incubating chamber got to 105 degrees F by accident [Re: SpitballJedi]
    #18804906 - 09/05/13 08:05 PM (10 years, 4 months ago)

It is still debated but I have left uninoculated jars for months at a time. I've never seen, smelled or had any idication of contams. The only thing that happens is they very slowly dry up.

I don't deny that endospores probably survive sterilization. That's not what I question.

I don't believe they actually recover in any amount of time that would be a threat to your jar...if the jar was properly PC'd and kept at good colonizing temps (86F is the max I've tried).

I left one jar out from each batch for a while, I saw no signs of bacteria after months of sitting at room temp or incubated.

Another argument against the endospore-recovery question is that a lot of people (including at least one TC I know of) prep their grains with no-soak preps. I have not heard of them having issues with bacteria because they did not germinate the endospores before PCing.

So...sure, endospores are there and dormant (damaged from the PC cycle) in your jar, I have no evidence that this is not true.

I just don't believe they will recover in time to pose a threat. Everything I've seen points to this being true.

My two cents on the topic :thumbup:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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InvisibleSpitballJedi
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18804929 - 09/05/13 08:12 PM (10 years, 4 months ago)

Quote:

FrankHorrigan said:
It is still debated but I have left uninoculated jars for months at a time. I've never seen, smelled or had any idication of contams. The only thing that happens is they very slowly dry up.




That was exactly my experience.

Your arguments are very reasonable and I believe them to be true. Thx for indulging me.


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Edited by SpitballJedi (09/05/13 08:37 PM)


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Offlinenepter627
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Re: incubating chamber got to 105 degrees F by accident [Re: SpitballJedi]
    #18805234 - 09/05/13 09:21 PM (10 years, 4 months ago)

built my tit today after reading how fast myc colonizes in it.

turned out the submersible heater i bought wasnt working nor heating my water.

(cheap walmart heater)
so tomorrow im going to return it and buy one with an adjustable tempature gauge.


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InvisibleFrankHorrigan
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Re: incubating chamber got to 105 degrees F by accident [Re: nepter627]
    #18805937 - 09/06/13 12:34 AM (10 years, 4 months ago)

Quote:

nepter627 said:
built my tit today after reading how fast myc colonizes in it.

turned out the submersible heater i bought wasnt working nor heating my water.

(cheap walmart heater)
so tomorrow im going to return it and buy one with an adjustable tempature gauge.




Make sure to get an air pump and an air stone/bubble wand.

It is very important to circulate that water, otherwise you will have hot spots in your incubator.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Offlinespacechildo
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18806452 - 09/06/13 07:53 AM (10 years, 4 months ago)

Quote:

FrankHorrigan said:
Quote:

nepter627 said:
built my tit today after reading how fast myc colonizes in it.

turned out the submersible heater i bought wasnt working nor heating my water.

(cheap walmart heater)
so tomorrow im going to return it and buy one with an adjustable tempature gauge.




Make sure to get an air pump and an air stone/bubble wand.

It is very important to circulate that water, otherwise you will have hot spots in your incubator.




This is probably why my tit failed! The heater kept turning off even though the water had turned cold.
It always failed after about a week.


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InvisibleMykes logos
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18806533 - 09/06/13 08:33 AM (10 years, 4 months ago)

i've always looked at it like it's a "race" between contams and myc.... so although leaving jars sitting un-inoculated isn't a big deal, I always try to nocc em up within a day or two. the contams are always there no matter what... you'd have to sterilize for like what, like 12 hours to completely kill off everything? so I try to go from plate to fruiting as fast as possible no matter what the situation is.. but I've rarely lost a jar over the years no matter what I was doing

I know a mycologist out west who soaks his rye 20-24 hours, then skips the boil altogether, and only sterilizes for 60 mins. After a few years of experimentation with many mediums, that's what he stuck with... but he also used wild rice and stuff like that a lot. there's a million ways to do it and they all work just the same. It's just like gardening or anything like this, ask 100 gardeners how to start seeds and you'll get 100 different answers..

What I really wanted to say tho is that I live in south FL and can set colonizing jars anywhere I want and anytime of the year without worrying about the temperature haha... ahhh living in the subtropics :sunny:


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Re: incubating chamber got to 105 degrees F by accident [Re: Mykes logos]
    #18806562 - 09/06/13 08:43 AM (10 years, 4 months ago)

I built one of Frank's TiTs to spec. The mycellium seem to grow like clockwork since I've switched to using one. Shake at one week from MS. 100% a week later.

I love my TiT!


Edited by CaptainPuffy (09/06/13 08:44 AM)


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InvisibleMykes logos
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Re: incubating chamber got to 105 degrees F by accident [Re: CaptainPuffy]
    #18806580 - 09/06/13 08:48 AM (10 years, 4 months ago)

Quote:

CaptainPuffy said:
I love my TiT!




haha... kudos to Frank for naming it that


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Offlinenepter627
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18808399 - 09/06/13 05:35 PM (10 years, 4 months ago)

Quote:

FrankHorrigan said:
Quote:

nepter627 said:
built my tit today after reading how fast myc colonizes in it.

turned out the submersible heater i bought wasnt working nor heating my water.

(cheap walmart heater)
so tomorrow im going to return it and buy one with an adjustable tempature gauge.




Make sure to get an air pump and an air stone/bubble wand.

It is very important to circulate that water, otherwise you will have hot spots in your incubator.





so should i not turn it on until i have an airpump and and air stone?


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InvisibleSpitballJedi
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Re: incubating chamber got to 105 degrees F by accident [Re: Mykes logos]
    #18808506 - 09/06/13 05:57 PM (10 years, 4 months ago)

Quote:

Mykes logos said:
Quote:

CaptainPuffy said:
I love my TiT!




haha... kudos to Frank for naming it that




TiT was around before Frank was a member


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Offlinenepter627
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Re: incubating chamber got to 105 degrees F by accident [Re: SpitballJedi]
    #18809996 - 09/06/13 11:42 PM (10 years, 4 months ago)

so i got a pump and a bubble stone.

now my question is my water in the tub suppose to erupt every 30=90 seconds?

water doesnt seem to be splashing over it was at first but i took some water out.

now it just erupts  thats the only word i can think of to describe whats happening.


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InvisibleFrankHorrigan
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Re: incubating chamber got to 105 degrees F by accident [Re: nepter627]
    #18810871 - 09/07/13 09:11 AM (10 years, 4 months ago)

Yep, I call it "the bloop" and it is how your water will be circulating.

No need to fill it so full it splashes. I keep my water about halfway up the side of my inner tote :thumbup:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Offlinenepter627
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Re: incubating chamber got to 105 degrees F by accident [Re: FrankHorrigan]
    #18811413 - 09/07/13 12:25 PM (10 years, 4 months ago)

Quote:

FrankHorrigan said:
Yep, I call it "the bloop" and it is how your water will be circulating.

No need to fill it so full it splashes. I keep my water about halfway up the side of my inner tote :thumbup:





okay great that means im doing something right, =]

and i releveled the water after the first splash and it has yet to splash so i think im good.


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Offlineghostinthemist
off in the distance i stand
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Registered: 08/01/13
Posts: 299
Last seen: 10 years, 4 months
Re: incubating chamber got to 105 degrees F by accident [Re: nepter627]
    #18855344 - 09/17/13 08:23 PM (10 years, 4 months ago)

IM BACK IN THE SADDLE AGAIN!


RUN AND HIDE!


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When all seems lost it has been neglected for too long to be found


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InvisibleDreaming Nomad
Psyborg
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Registered: 01/15/13
Posts: 908
Re: incubating chamber got to 105 degrees F by accident [Re: ghostinthemist]
    #18855422 - 09/17/13 08:45 PM (10 years, 4 months ago)

:somefunnyshit:

Hahahahahahahaha oh man, that is rich!


--------------------
They say curiosity killed the cat...
Fortunately, I am not a cat.


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