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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 1 month
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: Pestile]
#18804851 - 09/05/13 07:50 PM (10 years, 4 months ago) |
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Quote:
Pestilence said: Other guy can't break up spawn in jar, used spoon and had contaminants. This guy can't break up spawn in jar, used spoon and maybe has contaminants.
It's almost the same, no? 
It's exactly the same in every regard except for one is to be spawned to a sterile medium and the other is to be spawned to a pasteurized medium, which actually makes them worlds apart. A spoon that's been sent through the dishwasher is more than acceptable for the purpose of breaking up spawn for a bulk substrate. But when working with sterile substrate, nothing short of sterile is acceptable IMO.
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krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: Pestile]
#18804867 - 09/05/13 07:54 PM (10 years, 4 months ago) |
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Why us this not an issue when taking agar wedges and transferring to grain jars? We use tools for that.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: Psilicon]
#18804886 - 09/05/13 07:58 PM (10 years, 4 months ago) |
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Quote:
van der griegen said:
Quote:
Pestilence said: Other guy can't break up spawn in jar, used spoon and had contaminants. This guy can't break up spawn in jar, used spoon and maybe has contaminants.
It's almost the same, no? 
It's exactly the same in every regard except for one is to be spawned to a sterile medium and the other is to be spawned to a pasteurized medium, which actually makes them worlds apart. A spoon that's been sent through the dishwasher is more than acceptable for the purpose of breaking up spawn for a bulk substrate. But when working with sterile substrate, nothing short of sterile is acceptable IMO.
I agree.
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: krypto2000]
#18804974 - 09/05/13 08:27 PM (10 years, 4 months ago) |
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Quote:
krypto2000 said: Why us this not an issue when taking agar wedges and transferring to grain jars? We use tools for that.
scalpels are easily flame sterilized.
Using a sterilized spoon should only be used as a last ditch effort and one should understand the risk.
If you just can't get the grains broken up and you don't mind risking your materials and work and your only other option is to throw it away, then fuck it, use a sterilized spoon.
BUT under no circumstances wipe the spoon with IPA. Which is what the OP asked about.
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: SpitballJedi]
#18805283 - 09/05/13 09:30 PM (10 years, 4 months ago) |
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I just did some research for another thread and found that mycelium can survive past 10,000g without lysing. The limiting factor here is the jar, and I just think OP isn't being rough enough with it.
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krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: Psilicon]
#18806831 - 09/06/13 10:12 AM (10 years, 4 months ago) |
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Yeah, I find it *really* depends on what you beat it on, just listen for the sound as to whether the glass will break or not. I've found the best things to beat it on are either some ground outside or my hands/the dirt. You basically want something that will cushion the impact to spread the force throughout the jar, yet not in a central point enough that it will break the glass. I used to actually use a spoon for all of my work until I found this out, I used to simply think you couldn't break them up by banging it on things until I tried different materials.
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DeadPhan


Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: krypto2000]
#18807543 - 09/06/13 01:52 PM (10 years, 4 months ago) |
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Fully inflated bike tire is best. Down the line I have used phone books, (pretty nonexistent in parts these days) carpet, or the lawn. I've went to town on the ground or carpet when bike tire dont do it. Mason jars can take a beating.
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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rohr
silly cybin



Registered: 08/08/11
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Loc: TX
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: DeadPhan]
#18807669 - 09/06/13 02:29 PM (10 years, 4 months ago) |
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I find breaking up spawn literally exhausting.
I have to ask my big strong handsome man to break up my jars for me
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: rohr]
#18810098 - 09/07/13 12:33 AM (10 years, 4 months ago) |
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I did a batch of g2g with a spoon about 5 days ago, about 30+ jars, no contams so far. IME its about the same contam rate, maybe a tad higher but not bad.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 11 hours, 7 minutes
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: invitro]
#18810367 - 09/07/13 03:09 AM (10 years, 4 months ago) |
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Quote:
invitro said: I did a batch of g2g with a spoon about 5 days ago, about 30+ jars, no contams so far. IME its about the same contam rate, maybe a tad higher but not bad.
5 days isn't that long really. If your spawn got contaminated during a transfer it will generally take 10-35 days before you see any signs of it.
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: Kizzle]
#18810796 - 09/07/13 08:39 AM (10 years, 4 months ago) |
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I try to make it so my g2g jars finish colonizing after 5-7 days to prevent contams. I add a lot of colonized grain to each jar to ensure this happens.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: invitro]
#18810804 - 09/07/13 08:42 AM (10 years, 4 months ago) |
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Quote:
invitro said: I try to make it so my g2g jars finish colonizing after 5-7 days to prevent contams. I add a lot of colonized grain to each jar to ensure this happens
If your jars are contamming after only a week then you need to sterilize them better.
It should not be a "race" against the contams, there should be no contams.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: PussyFart]
#18810823 - 09/07/13 08:51 AM (10 years, 4 months ago) |
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I didn't say they were all contaming, but sometimes 10 or even 20% do after 10-14 days. According to RR's thoery that is how long it takes contams that were originally in the pced grains to wake up. So it is a race against the contams IMO. He says that the grains are not truly serilized after a pc run and that it would take 24 hours of continual pcing to truly sterilize a batch. I try to make it colonize as fast as possible as a precautionary approach, less to worry about I guess.
Edited by invitro (09/07/13 08:53 AM)
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: invitro]
#18810833 - 09/07/13 08:55 AM (10 years, 4 months ago) |
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Quote:
invitro said: I didn't say they were all contaming, but sometimes 10 or even 20% do after 10-14 days. According to RR's thoery that is how long it takes contams that were originally in the pced grains to wake up.
My grain jars stay contam free for a month or 2.
And that is not a theory, that is personal experience.
If your grain jars are contamming after only 2 weeks, something is not right.
Quote:
invitro said: So it is a race against the contams IMO.
If your mycellium has to race against contams, it has already lost.
Quote:
invitro said: He says that the grains are not truly serilized after a pc run and that it would take 24 hours of continual pcing to truly sterilize a batch.
Yes, but you do not need them to be 100% truly sterile, but at the same time they should not contaminate in 2 weeks time.
Just sayin....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: PussyFart]
#18810850 - 09/07/13 09:02 AM (10 years, 4 months ago) |
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Well I dunno what to say, I pc them at the recommended time and some of them do seem to contam within 2 weeks. The contam rate is low enough that's it's not that big a deal, but I usually just chalk it up to the fact that I have to use a SAB and not a flow hood, and the house I live in is in no way shape of form sterile, and due to the fact that I do a lot of work outside, often times with moldy stuff as part of my job, no way around this. I should pc some grains and leave them unmolested for 2 weeks and see what the contam rate is, that way I'll know if it's my pc procedure or my SAB.
I didn't mean to say "theory" it's RR's personal experience too I'm sure. How many jars have you ever let sit for 2 months and 100% of them stayed good?
Edited by invitro (09/07/13 09:06 AM)
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: invitro]
#18810855 - 09/07/13 09:05 AM (10 years, 4 months ago) |
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Quote:
invitro said: The contam rate is low enough that's it's not that big a deal, but I usually just chalk it up to the fact that I have to use a SAB and not a flow hood, and the house I live in is in no way shape of form sterile, and due to the fact that I do a lot of work outside, often times with moldy stuff as part of my job, no way around this.
I only work in a SAB and have just about 100% success rate, with no plans on building a flow hood anytime soon.
Sounds like your sterile procedure needs some work.
Quote:
invitro said: I should pc some grains and leave them unmolested for 2 weeks and see what the contam rate is, that way I'll know if it's my pc procedure or my SAB.
Yes....right on....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: PussyFart]
#18810965 - 09/07/13 09:36 AM (10 years, 4 months ago) |
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With most contams all is lost, esp if it's green or cobweb mold, but I just had a jar of myc win a battle against bacteria. some grains on the bottom were wet and greasy looking with white blotches. The rest of the jar was good myc. For about 2 weeks each party held their own ground but eventually the myc took over to my surprise. I think the jar dried out a bit making conditions harder for the bacteria.
I know what you meant though, that when you see contams the practical thing to do is pitch it. Even if you could win out it's not practical to wait an extra 2 weeks. But when I said race against contams I didn't mean 2 active networks racing aginst each other, I meant active mushrrom myc racing to colonize before the endospores wake up from their pc induced coma.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: invitro]
#18811908 - 09/07/13 03:07 PM (10 years, 4 months ago) |
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Quote:
I didn't say they were all contaming, but sometimes 10 or even 20% do after 10-14 days.
Bacteria?
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magnesium
Stranger


Registered: 08/08/13
Posts: 31
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: Kizzle]
#18827079 - 09/11/13 09:37 AM (10 years, 4 months ago) |
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Update: It's been about a week and the jars are almost done. No sign of contams yet. G2g seems like a great way to get spawn
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: magnesium]
#18828170 - 09/11/13 02:54 PM (10 years, 4 months ago) |
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All the ones I did are still good too.
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