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magnesium
Stranger

Registered: 08/08/13
Posts: 31
Last seen: 9 years, 8 months
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g2g: Master jar won't break up. Can I use a spoon?
#18802768 - 09/05/13 10:38 AM (10 years, 4 months ago) |
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This is my first g2g. I have an older jar that I want to turn into 8 jars. The master jar is one giant clump and will not break even after 20 minutes of hitting it on things 
Can I just wipe some IPA on the spoon and dig it out into my receiving jars?
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ghostinthemist
off in the distance i stand



Registered: 08/01/13
Posts: 299
Last seen: 10 years, 4 months
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: magnesium]
#18802802 - 09/05/13 10:48 AM (10 years, 4 months ago) |
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yes.
-------------------- When all seems lost it has been neglected for too long to be found
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Pestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: magnesium]
#18802815 - 09/05/13 10:54 AM (10 years, 4 months ago) |
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   The Corbett Report Open Source Intelligence News
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ghostinthemist
off in the distance i stand



Registered: 08/01/13
Posts: 299
Last seen: 10 years, 4 months
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: Pestile]
#18802844 - 09/05/13 11:01 AM (10 years, 4 months ago) |
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Using your hand will not be any better. You could just beat the hell out of it for another 20 mins. Dont use your hand without a clean glove though. Nothing but contams on hands
-------------------- When all seems lost it has been neglected for too long to be found
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: magnesium]
#18802855 - 09/05/13 11:04 AM (10 years, 4 months ago) |
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Quote:
magnesium said: Can I just wipe some IPA on the spoon and dig it out into my receiving jars?
You can if you want to increase your chance of failure.
If you must use a spoon, then wrap it in foil and PC it 30 mins. You may can do it for less time, but I would do it for 30. After you PC the spoon, unwrap it in your SAB or flowhood. Do not wipe it with IPA.
IPA will only sanitize the spoon. Your master jar is contam resistant, but your new sterile jar is not. If you just wipe the spoon with IPA, you have a high risk of contaminating the new jars.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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DeadPhan


Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: SpitballJedi]
#18802979 - 09/05/13 11:38 AM (10 years, 4 months ago) |
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Couldn't you just flame sterilize the spoon in that case?
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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magnesium
Stranger

Registered: 08/08/13
Posts: 31
Last seen: 9 years, 8 months
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: DeadPhan]
#18803039 - 09/05/13 11:49 AM (10 years, 4 months ago) |
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Quote:
DeadPhan said: Couldn't you just flame sterilize the spoon in that case?
That's what I was thinking
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: magnesium]
#18803100 - 09/05/13 12:05 PM (10 years, 4 months ago) |
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Using any tool for grain-to-grain on to a sterile media will inevitably result in a high rate of failure.
Even a flame-sterilized spoon.
Grain to grain needs to be kept 100% aseptic. This is hard enough when using proper technique, let alone when you're flailing about the jar with a spoon 
OP, if your jar is so old that the myc won't break up after some hard pounding on a telephone book or bike tire, odds are it will not perform well for much. I would just fruit the grains as they are, break them up and case them.
How old is this jar?
It may also be contam'd, they will fruit invitro if they are clean.
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ghostinthemist
off in the distance i stand



Registered: 08/01/13
Posts: 299
Last seen: 10 years, 4 months
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: FrankHorrigan]
#18803159 - 09/05/13 12:23 PM (10 years, 4 months ago) |
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Take the grains and directly mix them with a sub start a new master
-------------------- When all seems lost it has been neglected for too long to be found
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magnesium
Stranger

Registered: 08/08/13
Posts: 31
Last seen: 9 years, 8 months
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: FrankHorrigan]
#18803172 - 09/05/13 12:26 PM (10 years, 4 months ago) |
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Quote:
FrankHorrigan said: Using any tool for grain-to-grain on to a sterile media will inevitably result in a high rate of failure.
Even a flame-sterilized spoon.
Grain to grain needs to be kept 100% aseptic. This is hard enough when using proper technique, let alone when you're flailing about the jar with a spoon 
OP, if your jar is so old that the myc won't break up after some hard pounding on a telephone book or bike tire, odds are it will not perform well for much. I would just fruit the grains as they are, break them up and case them.
How old is this jar?
It may also be contam'd, they will fruit invitro if they are clean.
Not too sure, but it's probably been fully colonized for about 6 weeks. I may just go ahead and crumble em and case them.
I have 7 jars that are ready to go g2g. Are these now useless? I don't have any syringes or spores to innoc them with.
Lastly, would there be any use in trying to g2g the jars (since I probably have no use for them now since I will be casing them)anyways. I have 2 quarts that I was going to g2g eventually. Maybe case 1.5 quarts and then try a g2g with the remaining .5 quart?
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: magnesium]
#18803187 - 09/05/13 12:31 PM (10 years, 4 months ago) |
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You would want to grain to grain the half quart first and then you can safely take it out and case the rest in open air.
If you have the materials and you are willing to accept some failure, I say go for it. Experimenting is the best way to learn something.
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ghostinthemist
off in the distance i stand



Registered: 08/01/13
Posts: 299
Last seen: 10 years, 4 months
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: magnesium]
#18803249 - 09/05/13 12:52 PM (10 years, 4 months ago) |
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I don't understand the g2g theory anyway. Is it that you are introducing colonized myce to an sub because it will take over quicker? how much faster is g2g than multi spore?
-------------------- When all seems lost it has been neglected for too long to be found
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: ghostinthemist]
#18803271 - 09/05/13 12:58 PM (10 years, 4 months ago) |
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usually way faster. You use a jar of colonized grain to inoculate more jars. You don't have to wait for the germination process.
So, my PC holds 7 jars. Once colonized, I can easily g2g each of those 7 to 7 more to make a total of 49 jars and so on.
This saves my spores as well as allow me to fruit many monotubs with genetics I like.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
Edited by SpitballJedi (09/05/13 12:59 PM)
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DeadPhan


Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: ghostinthemist]
#18803278 - 09/05/13 12:59 PM (10 years, 4 months ago) |
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Quote:
ghostinthemist said: I don't understand the g2g theory anyway. Is it that you are introducing colonized myce to an sub because it will take over quicker? how much faster is g2g than multi spore?
Much faster. With multispore you have to wait for spores to germinate. With grain to grain you are introducing mycelium, not spores.
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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jonnymushroom
the man of many teks

Registered: 07/27/13
Posts: 262
Loc: dugg in somewere
Last seen: 9 years, 10 months
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: magnesium]
#18803504 - 09/05/13 02:12 PM (10 years, 4 months ago) |
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Get to a clean work area with a your glove box. Torch the spoon good let it cool off in the box on something sterile and use caution breaking up the myc. by keeping all thing but the sterile spoon away from around the jar lid to be successful .
good luck
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: jonnymushroom]
#18803511 - 09/05/13 02:13 PM (10 years, 4 months ago) |
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Trust me, my man, it is almost guaranteed to fail.
Your hands, sleeves, arm, etc will be nearby. Even with sanitized gloves and tyvek sleeves you'll be kicking stuff around in the air as you are moving in the SAB.
Don't use tools for G2G for best chances of success
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magnesium
Stranger

Registered: 08/08/13
Posts: 31
Last seen: 9 years, 8 months
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: FrankHorrigan]
#18803599 - 09/05/13 02:39 PM (10 years, 4 months ago) |
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OP here. I PC'd the spoon for about 30 minutes and g2g to 7 jars. I used the spoon only at the beginning to stir up the master jar. It actually broke up quite easily with the spoon, I just couldn't get it broken up by hitting things.
Things were quite clunky my first time. I'll update this thread in a few days.
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jonnymushroom
the man of many teks

Registered: 07/27/13
Posts: 262
Loc: dugg in somewere
Last seen: 9 years, 10 months
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: FrankHorrigan]
#18803708 - 09/05/13 03:17 PM (10 years, 4 months ago) |
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Yea there's high odds for failure, but it can be done by (some) because I have done it but it's not a walk in the park and not fun either .
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 12 hours, 54 minutes
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: Pestile]
#18804136 - 09/05/13 04:54 PM (10 years, 4 months ago) |
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Quote:
Pestilence said: Breaking up spawn jars with a spoon; Cause of contamination?
Read this first.
That's different really. The link is a question about breaking grains for spawning and not for g2g to another jar. Either way a spoon shouldn't be necessary to break up a properly prepared grain jar.
I definitely agree with Frank that
Quote:
Don't use tools for G2G for best chances of success 
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Pestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
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Re: g2g: Master jar won't break up. Can I use a spoon? [Re: Kizzle]
#18804387 - 09/05/13 06:00 PM (10 years, 4 months ago) |
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Quote:
Kizzle said:
Quote:
Pestilence said: Breaking up spawn jars with a spoon; Cause of contamination?
Read this first.
That's different really. The link is a question about breaking grains for spawning and not for g2g to another jar. Either way a spoon shouldn't be necessary to break up a properly prepared grain jar.
It was meant to say the same as this quote;
Quote:
Don't use tools for G2G for best chances of success 
Other guy can't break up spawn in jar, used spoon and had contaminants. This guy can't break up spawn in jar, used spoon and maybe has contaminants.
It's almost the same, no?
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   The Corbett Report Open Source Intelligence News
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