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OfflineFarmerwhite
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Registered: 02/03/13
Posts: 161
Last seen: 4 years, 10 months
Need some monotub advice
    #18802308 - 09/05/13 07:11 AM (10 years, 4 months ago)

Hey guys, I was curious what I should do. I have 6 qt jars of PE colonizing very quickly and I also have 1 KSSS due to the others not germinating.

Should I spawn all 6 quarts to a single 64 qt monotub or should I spawn them to multiple tubs for highest yield?

Also what should I do with my 1 qt of KSSS? I have never done g2g and I am unsure of what kind if sterile conditions I can provide for long periods.


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Invisiblejboredone
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Re: Need some monotub advice [Re: Farmerwhite]
    #18802318 - 09/05/13 07:19 AM (10 years, 4 months ago)

I suggest you g2g those jars to 60 or so other jars and then attempt to take over the world........:awethumb:


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OfflineFarmerwhite
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Re: Need some monotub advice [Re: jboredone]
    #18802326 - 09/05/13 07:21 AM (10 years, 4 months ago)

If that was something I was confident in doing it would have already been done but I've worked hard to get here and I don't want to throw it away now with contamination due to shift procedure


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Offlineklak1212
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Re: Need some monotub advice [Re: Farmerwhite]
    #18802330 - 09/05/13 07:22 AM (10 years, 4 months ago)

Quote:

Farmerwhite said:
Hey guys, I was curious what I should do. I have 6 qt jars of PE colonizing very quickly and I also have 1 KSSS due to the others not germinating.

Should I spawn all 6 quarts to a single 64 qt monotub or should I spawn them to multiple tubs for highest yield?

Also what should I do with my 1 qt of KSSS? I have never done g2g and I am unsure of what kind if sterile conditions I can provide for long periods.




Spawn all 6 qt jars of PE to one tub, wash the jars out and make more jars of grain. Then G2G the KSSS jar to the 6 new qt jars. Look into building a SAB (Still Air Box), and follow Franks tek on doing G2G (Franks Tek, click here)


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InvisiblePestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
Re: Need some monotub advice [Re: Farmerwhite]
    #18802331 - 09/05/13 07:24 AM (10 years, 4 months ago)

Don't be scared! Live and learn! :thumbup:


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Invisiblejboredone
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Re: Need some monotub advice [Re: klak1212]
    #18802334 - 09/05/13 07:27 AM (10 years, 4 months ago)

g2g isn't a very difficult process....:jah:


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OfflineFarmerwhite
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Re: Need some monotub advice [Re: jboredone]
    #18802351 - 09/05/13 07:35 AM (10 years, 4 months ago)

Is it very easy to get contams?


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InvisiblePestile

Registered: 05/02/13
Posts: 875
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Re: Need some monotub advice [Re: Farmerwhite]
    #18802478 - 09/05/13 08:34 AM (10 years, 4 months ago)

If you do it in a SAB there shouldn't be a problem with contams.

How to do Grain to Grain (G2G) Transfers in a Still-Air Box (SAB) Video



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OfflineMush4Brains
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Re: Need some monotub advice [Re: jboredone]
    #18802485 - 09/05/13 08:37 AM (10 years, 4 months ago)

Quote:

jboredone said:
I suggest you g2g those jars to 60 or so other jars and then attempt to take over the world........:awethumb:





This is such useless advice.  What are you going to do with 60 quarts, run ten tubs at once?  This is asking for a massive failure.


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OfflineFarmerwhite
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Re: Need some monotub advice [Re: Mush4Brains]
    #18802519 - 09/05/13 08:55 AM (10 years, 4 months ago)

Thank you for that video, it was nice actually seeing how easy that is. Do you happen to have a SAB construction tek? Drilling holes in plastic tubs intimidates me only because I don't have a drill


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OfflineMush4Brains
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Re: Need some monotub advice [Re: Farmerwhite]
    #18802535 - 09/05/13 09:07 AM (10 years, 4 months ago)

You can heat up an empty aluminum can on the stove and melt holes as well.  The holes aren't the greatest, but it works.

Literally an SAB tek is putting holes in a tub.  That's it.  Some people like to add tyvek sleeves and PVC to the holes to help prevent drafts.

Here is TL's pictorial

http://www.shroomery.org/forums/showflat.php/Number/14722730#14722730


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Invisibletbagtag
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Registered: 01/16/13
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Re: Need some monotub advice [Re: Mush4Brains]
    #18802547 - 09/05/13 09:11 AM (10 years, 4 months ago)

You can also click the grow along link in my  signature I have a step by step for grain to grain.


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InvisiblePestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
Re: Need some monotub advice [Re: Farmerwhite]
    #18802565 - 09/05/13 09:15 AM (10 years, 4 months ago)

Like this:

Quote:

Notahacker420 said:
If you are getting a 75% success rate on agar in a SAB, your sterile technique needs some fine tuning, not the SAB.

A flow hood is not going to fix this.

I get 99-100% success rate in my SAB, with no thought of building a flow hood anytime soon.

   




From: http://www.shroomery.org/forums/showflat.php/Number/18528520#18528520

Or do it like this:



From: http://www.shroomery.org/forums/showflat.php/Number/18399390#18399390


These are just pictures, but the basic idea is to have a plastic tub with two holes for the arms. You can use the one with the lid on or turn it upside down and lay a moist towel underneath to catch the whatever particles that lands on it. Keep sides moist for the same reason. That's it. You can even take a cardboard box and cut some holes in it, make a saran wrap window and you got yourself a SAB. :smile:


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OfflineMush4Brains
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Re: Need some monotub advice [Re: Pestile]
    #18802589 - 09/05/13 09:25 AM (10 years, 4 months ago)

My first ghetto cardboard SAB treated me well.  I used clear packing tape instead of saran wrap.


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InvisibleSpitballJedi
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Registered: 10/13/12
Posts: 8,598
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Re: Need some monotub advice [Re: Farmerwhite]
    #18802771 - 09/05/13 10:39 AM (10 years, 4 months ago)

Quote:

Farmerwhite said:
Thank you for that video, it was nice actually seeing how easy that is. Do you happen to have a SAB construction tek? Drilling holes in plastic tubs intimidates me only because I don't have a drill





How I made my SAB. If you don't ave a drill, you will have to figure out a different way to make the arm holes.


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Offlineklak1212
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Re: Need some monotub advice [Re: SpitballJedi]
    #18802983 - 09/05/13 11:38 AM (10 years, 4 months ago)

Draw holes with pencil, then torch lighter.


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Invisibletbagtag
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Registered: 01/16/13
Posts: 1,432
Loc: Amsterdam Flag
Re: Need some monotub advice [Re: SpitballJedi]
    #18803060 - 09/05/13 11:55 AM (10 years, 4 months ago)

Quote:

SpitballJedi said:
Quote:

Farmerwhite said:
Thank you for that video, it was nice actually seeing how easy that is. Do you happen to have a SAB construction tek? Drilling holes in plastic tubs intimidates me only because I don't have a drill





How I made my SAB. If you don't ave a drill, you will have to figure out a different way to make the arm holes.




use a torch and soup can.  Be careful some post a while back thought the fucking can would burn his house down...


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OfflineFarmerwhite
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Re: Need some monotub advice [Re: tbagtag]
    #18803664 - 09/05/13 03:01 PM (10 years, 4 months ago)

Also will Damion's bucket tech suffice as long as I make sure to water is between 170 and 180?


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InvisibleGretchenmeister
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Registered: 07/23/05
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Re: Need some monotub advice [Re: Farmerwhite]
    #18803681 - 09/05/13 03:08 PM (10 years, 4 months ago)

Quote:

Farmerwhite said:
If that was something I was confident in doing it would have already been done but I've worked hard to get here and I don't want to throw it away now with contamination due to shift procedure




Try closed container myc transfer with the KSSS :smile:  Pc a jar of water with a filter covered hole and an injection site, sterilize a syringe, suck clean water up, shoot in colonized KSSS jar, swirl around a bit (I dont shake like the grain lc tek), suck back out and noc up some lc and/or several more grain jars.  My original jar is still very much able to be used for spawn purposes when the other jars catch up. They usually colonize fairly quickly using this technique.


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: Need some monotub advice [Re: Mush4Brains]
    #18803686 - 09/05/13 03:10 PM (10 years, 4 months ago)

Quote:

Mush4Brains said:
Quote:

jboredone said:
I suggest you g2g those jars to 60 or so other jars and then attempt to take over the world........:awethumb:





This is such useless advice.  What are you going to do with 60 quarts, run ten tubs at once?  This is asking for a massive failure.



Jboredone.  You need to realize before you critique, that g2g is a very viable method that is utilized by more than a few cultivators on this forum.  Yes, with that much spawn you could make 10 tubs, and yes it has been done in the past, and is currently being utilized as a method by more than a few of us with great success.

I actually think the advice of spawning the PE, and G2G the KSS is a great idea.  Yes you will need a still air box or flow hood, and yes it is a very viable method. 


Good luck in your future endeavors.


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OfflineMush4Brains
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Re: Need some monotub advice [Re: HypnotoadCroaked]
    #18803748 - 09/05/13 03:28 PM (10 years, 4 months ago)

Yeah, for someone who has never done grain to grain and asking for monotub advice?  Recipe for disaster.  Of course there are people on here running ten tubs each time, but that doesn't mean it is proper advice for someone who wants advice on only one.


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InvisibleHypnotoadCroaked
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Re: Need some monotub advice [Re: Mush4Brains]
    #18804202 - 09/05/13 05:09 PM (10 years, 4 months ago)

Quote:

Mush4Brains said:
Yeah, for someone who has never done grain to grain and asking for monotub advice?  Recipe for disaster.  Of course there are people on here running ten tubs each time, but that doesn't mean it is proper advice for someone who wants advice on only one.



I was more than half asleep when I responded.  I can see your point (but still respectfully disagree).  Your previous post made it sound simply like what was suggested was impossible, which is simply not true.  Something as simple as a $5 tub with 2 arm holes can increase success of everything we do exponentially.  It is borderline preposterous to think of not owning one after a few grows.  The SAB opens a number of doors to other methods that are 100% impossible in open air, AND creates a control situation.  Doing things in a sab will remove the variables that open air causes.  Having a SAB almost totally removes the need to ever order another spore syringe ever.

While you have a valid point, as others mentioned....G2g is not hard at all.


My opinion on grain:

The hardest part of using grain for our hobby is proper hydration.  If you can dump grain into a mono-tub with bulk substrate, one can surely dump grain into another jar in a SAB with equal success.  A majority of the techniques used in this hobby are not hard in any way, but simply require simple attention to detail....The hardest (IMHO) Is still verifying that grains are not too wet or dry. 

I still agree full with Klak1212.  Spawn a full tub with 6 jars, and (As pestilence stated) g2g the remaining jar.  You will learn something, and even if you fail 50% you will still have 3 jars of colonized kss.  This is better than one, and in a few weeks, you can have a second tub ready to go.


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Invisiblejboredone
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Re: Need some monotub advice [Re: Mush4Brains]
    #18806264 - 09/06/13 05:36 AM (10 years, 4 months ago)

Quote:

Mush4Brains said:
Yeah, for someone who has never done grain to grain and asking for monotub advice?  Recipe for disaster.  Of course there are people on here running ten tubs each time, but that doesn't mean it is proper advice for someone who wants advice on only one.



The OP asked if he should  spawn to one tub or make multiple tubs....I simply said to g2g and make more tubs....It isn't very difficult at all....."nobody has ever done anything until the first time"....If you think that he is gonna fail just because it is his first time then you are an idiot.........:justdontknow:


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