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Offlinecrimsonking91
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Registered: 02/19/13
Posts: 188
Last seen: 10 years, 1 month
easy clone lc?
    #18797713 - 09/04/13 01:38 AM (10 years, 4 months ago)

Does anyone think it would work and be easier to take the plunger out of a sterile syringe, cut some tissue sample and drop it in the syringe in the back, put the plunger back in and suck up some sterilized honey lc. I figure the syringe would be done in a week. You could even shake it up by drawing  in air. I don't know if that would lead to contams by pulling in air and shaking up mycelium. Keep in mind entire operation would be done in glovebox very sterile.


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Invisiblewildernessjunkie
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I'm a teapot


Registered: 06/13/10
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Re: easy clone lc? [Re: crimsonking91]
    #18797724 - 09/04/13 01:49 AM (10 years, 4 months ago)

Chances of that working are pretty slim.


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OfflineMush4Brains
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Registered: 07/31/13
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Re: easy clone lc? [Re: wildernessjunkie]
    #18797744 - 09/04/13 02:01 AM (10 years, 4 months ago)



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Offlinecrimsonking91
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Registered: 02/19/13
Posts: 188
Last seen: 10 years, 1 month
Re: easy clone lc? [Re: wildernessjunkie]
    #18797745 - 09/04/13 02:01 AM (10 years, 4 months ago)

I don't see why its much different from shooting clone tissue into an lc, its just a matter of being quick and sterile. I want to try this because you can get a bigger piece of tissue to colonize less water


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OfflineMush4Brains
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Re: easy clone lc? [Re: Mush4Brains]
    #18797756 - 09/04/13 02:07 AM (10 years, 4 months ago)

You don't need a bigger piece of tissue.  The reason why it is different is because you might as well take a tissue sample and drop it straight into your LC instead of taking the plunger out of a syringe then putting it back in (you probably won't have enough hands to do this easily).  Then you want to draw in air?  You're just asking for contamination.  Why make things harder?


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Offlinecrimsonking91
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Registered: 02/19/13
Posts: 188
Last seen: 10 years, 1 month
Re: easy clone lc? [Re: Mush4Brains]
    #18797760 - 09/04/13 02:10 AM (10 years, 4 months ago)

Yea the original seems the way to go sorry for wasting anyone's time


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