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OfflineExcel89
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Registered: 08/27/13
Posts: 21
Last seen: 10 years, 4 months
Is this mycelium even growing?
    #18795463 - 09/03/13 05:30 PM (10 years, 4 months ago)

I used spore prints to inoculate 10 of these about 2 weeks ago. I don't see much growth, and can't tell if it's inoculated or if there's contamination or if it's not even growing at all. What do you guys think?





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InvisiblePestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
Re: Is this mycelium even growing? [Re: Excel89]
    #18795478 - 09/03/13 05:33 PM (10 years, 4 months ago)

What is it?


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Invisibletbagtag
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Registered: 01/16/13
Posts: 1,432
Loc: Amsterdam Flag
Re: Is this mycelium even growing? [Re: Excel89]
    #18795480 - 09/03/13 05:34 PM (10 years, 4 months ago)

Is that supposed to be agar?


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OfflinePhosCap
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Re: Is this mycelium even growing? [Re: Excel89]
    #18795485 - 09/03/13 05:35 PM (10 years, 4 months ago)

What is that? what did you inoculate?


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Offlineinvitro


Registered: 05/03/13
Posts: 2,529
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Re: Is this mycelium even growing? [Re: Excel89]
    #18795495 - 09/03/13 05:36 PM (10 years, 4 months ago)

That looks like a highly unusual situation, what are you trying to do ? :badsurprise:


Edited by invitro (09/03/13 05:41 PM)


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OfflineExcel89
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Re: Is this mycelium even growing? [Re: PhosCap]
    #18795500 - 09/03/13 05:37 PM (10 years, 4 months ago)

Agar, I used the grocery store tek with potato flakes, agar, honey, and water.


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Offlineslh980
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Re: Is this mycelium even growing? [Re: PhosCap]
    #18795501 - 09/03/13 05:37 PM (10 years, 4 months ago)

that looks weird... i mean yeah its agar, but a) its in a tupper ware container... and b) ew.....


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Offlineinvitro


Registered: 05/03/13
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Re: Is this mycelium even growing? [Re: slh980]
    #18795512 - 09/03/13 05:39 PM (10 years, 4 months ago)

Did you snap down the lid tight?  doesn't look like it, and why is there a big dark line on the agar in the one pic?  Did that grow after you inoculated?


Edited by invitro (09/03/13 05:40 PM)


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Offlineslh980
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Re: Is this mycelium even growing? [Re: invitro]
    #18795525 - 09/03/13 05:42 PM (10 years, 4 months ago)

Quote:

invitro said:
Did you snap down the lid tight?  doesn't look like it, and why is there a big dark line on the agar in the one pic?  Did that grow after you inoculated?




my guess is yeah, that lines in there and grew after. even the first pic has weird black dots everywhere....


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Invisibletbagtag
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Re: Is this mycelium even growing? [Re: slh980]
    #18795532 - 09/03/13 05:44 PM (10 years, 4 months ago)

Ok, no you have mold.  I had this same problem.  I'm a little over a month into agar and here is what I can tell you based off my fails.  I had issues that look like yours.  So here it goes:

Look like cobweb or mildew, when I used the tek you're using I had this same issue.

Switch to petris, you need the co2 build up.

Also use as little spore in the center as possible.

Try lme agar, I switched to that and have had much better success.


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InvisibleMykes logos
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Registered: 08/05/12
Posts: 1,108
Loc: FL Flag
Re: Is this mycelium even growing? [Re: invitro]
    #18795555 - 09/03/13 05:50 PM (10 years, 4 months ago)

the agar is too thick, you're using the wrong container, and the agar doesn't look like it was mixed very well in the first place.... and that's mold/bacteria fosho :frown:

Also, the "spore drop" inoculation technique tends to result in very clumpy and inconsistent spores on the agar.

IMO/E for next time: just buy pre-mixed agar, disposable petris (cynmar.com sells em cheap), and an inoculation loop. You'll save a lot of time and effort, and really only spend $40 or $50 from start to finish.

just wondering, what was your recipe? how long did you sterilize? and was this a "no-pour" tek?

cheers


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Offlineinvitro


Registered: 05/03/13
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Re: Is this mycelium even growing? [Re: Mykes logos]
    #18795634 - 09/03/13 06:13 PM (10 years, 4 months ago)

I have a home-made inoculation loop, I got some thick copper wire and bent the tip with pliers, works great.  Strip the rubber off the wire with a paring knife or wire stippers if it's not bare already.

I started by making my own agar, if you can afford it do yourself a favor and buy them pre-made, it saves a lot of time.


Edited by invitro (09/03/13 06:16 PM)


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OfflineExcel89
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Re: Is this mycelium even growing? [Re: Mykes logos]
    #18795758 - 09/03/13 06:45 PM (10 years, 4 months ago)

Thanks Mykes, I just placed an order with them and ordered 20 premixed sterilized petri dishes as well as a loop and some extra petri dishes.

I used the grocery store tek because I had trouble finding malt extract for the agar, used a pressure cooker for about an hour, and I did have to pour it (And didn't use a glove box which may have been the problem), since the plastic would have melted. I thought sterilizing it with disinfectant and hydrogen peroxide would have been enough. I just made myself a glove box about an hour ago, and did another test. This time I'm trying to make LC. As I mentioned, the spore prints/syringes that I have right now are ones I made about a year ago and just left in my attic. They weren't properly stored either so it's very possible they're all contaminated. I'm just trying to see if I can salvage anything before I spend another $60 bucks to buy a few spore syringes online.


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InvisibleMykes logos
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Registered: 08/05/12
Posts: 1,108
Loc: FL Flag
Re: Is this mycelium even growing? [Re: Excel89]
    #18801322 - 09/04/13 10:10 PM (10 years, 4 months ago)

Here's a tip:

Learn how to do sterile work with plates, slants, etc., and avoid LCs. Using agar will change your life.

Inoculate your mind and become sustainable. One MS syringe or LC could last a lifetime if you do it right.

Cheers


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