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MushroomGrowOp

Registered: 04/23/12
Posts: 93
Last seen: 6 years, 6 months
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White fuzz growing on top of mycelium on agar
#18788849 - 09/02/13 09:30 AM (10 years, 5 months ago) |
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I have four agar dishes that are all 75% colonized but it seems that somehow mold is growing over the mycelium. All of the exposed agar is clean and the mold seems to have started from where the tissue transfer was from.
Is it possible to spray these dishes lightly with an H2O2 solution in front of a flow hood and transfer several more time to clean it up. I only have a few cultures available to me at the moment and it is really important to save this one if possible.
If the H2O2 solution idea will work at what concentration should I use.
Thanks for youre help!
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Trippy_Penguin



Registered: 03/18/12
Posts: 624
Last seen: 3 years, 5 months
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Re: White fuzz growing on top of mycelium on agar [Re: MushroomGrowOp]
#18788963 - 09/02/13 10:03 AM (10 years, 5 months ago) |
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If the mold hasn't completely taken over the dish, you could transfer some clean growth and toss the dish.
Edit: I'd just do this in a SAB and be precise about it.
Edited by Trippy_Penguin (09/02/13 10:04 AM)
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MushroomGrowOp

Registered: 04/23/12
Posts: 93
Last seen: 6 years, 6 months
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Re: White fuzz growing on top of mycelium on agar [Re: Trippy_Penguin]
#18789069 - 09/02/13 10:36 AM (10 years, 5 months ago) |
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The SAB is a good idea. The only reason I ask about the H2O2 is because the mold growth is soooo close to the leading edge of mycelium. Do you think placing a drop of H2O2 with a syringe on the area I plan to transfer a good idea to try and get a clean piece...
Edit: I think I will just try both. I have plenty of agar plates. Ill try it with and without and see what happens.
Edited by MushroomGrowOp (09/02/13 10:39 AM)
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GoldenArrow
Chasing self up spiral staircase



Registered: 10/05/11
Posts: 1,370
Loc: UK
Last seen: 5 years, 11 months
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Re: White fuzz growing on top of mycelium on agar [Re: Trippy_Penguin]
#18789072 - 09/02/13 10:37 AM (10 years, 5 months ago) |
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What kind of myc is it and do you have any pics of the plates?
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MushroomGrowOp

Registered: 04/23/12
Posts: 93
Last seen: 6 years, 6 months
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Re: White fuzz growing on top of mycelium on agar [Re: GoldenArrow]
#18789088 - 09/02/13 10:42 AM (10 years, 5 months ago) |
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No pics sorry. You can see the rysomorphic growth on the bottom and a layer of fuzz that is growing over the top. The myc is starting to barely out grow it.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 12 hours, 18 minutes
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Re: White fuzz growing on top of mycelium on agar [Re: MushroomGrowOp]
#18789143 - 09/02/13 10:56 AM (10 years, 5 months ago) |
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try to transfer it a few times
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: White fuzz growing on top of mycelium on agar [Re: MushroomGrowOp] 1
#18789151 - 09/02/13 10:58 AM (10 years, 5 months ago) |
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Transfer to different types of agar media.
I have seen this kind of thing, mold "piggybacking" your mycelium. The only thing to do is to transfer to different types of agar and hope your healthy mycelium will grow out in another direction from the mold.
This may take a few transfers. If you are not worried about this particular culture you may want to start over from spores again.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 12 hours, 18 minutes
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Re: White fuzz growing on top of mycelium on agar [Re: FrankHorrigan]
#18789200 - 09/02/13 11:09 AM (10 years, 5 months ago) |
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lol ya i was going to say start over but didnt sound like he wanted too
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MushroomGrowOp

Registered: 04/23/12
Posts: 93
Last seen: 6 years, 6 months
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Re: White fuzz growing on top of mycelium on agar [Re: FrankHorrigan]
#18789223 - 09/02/13 11:11 AM (10 years, 5 months ago) |
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Do you think making an agar plate that has the peroxide incorpated will help?
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 12 hours, 18 minutes
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Re: White fuzz growing on top of mycelium on agar [Re: MushroomGrowOp]
#18789237 - 09/02/13 11:14 AM (10 years, 5 months ago) |
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no
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: White fuzz growing on top of mycelium on agar [Re: MushroomGrowOp]
#18789251 - 09/02/13 11:16 AM (10 years, 5 months ago) |
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I very much doubt it, but who knows?
This may not apply to you because I have easy access to tons of grain-dunk water but...
I had good results in getting my mycelium to distinguish itself on agar when I used grain-dunk agar.
It is made from the leftover water from rehydrating the fully colonized grains...in a sense, it is a bit like making agar from a GLC. There are a lot of metabolites and bits of mycelium and small amounts of nutrients from the grains themselves.
500mL grain-dunk water, 10g agar.
I'm not sure if you have this grain-dunk water accessible all the time like I do but it is the only thing I've transferred to (so far) that helped me save a culture that was growing alongside an intruder like you describe.
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MushroomGrowOp

Registered: 04/23/12
Posts: 93
Last seen: 6 years, 6 months
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Re: White fuzz growing on top of mycelium on agar [Re: FrankHorrigan]
#18789286 - 09/02/13 11:25 AM (10 years, 5 months ago) |
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Frank thank you very much. I will prepare some plates the way you describe and see what happens.
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MushroomGrowOp

Registered: 04/23/12
Posts: 93
Last seen: 6 years, 6 months
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Re: White fuzz growing on top of mycelium on agar [Re: MushroomGrowOp]
#18811100 - 09/07/13 10:28 AM (10 years, 5 months ago) |
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UPDATE:
I decided to let the dishes be and isolated them just to see what happened. I came back a few days later to see that the mycelium was growing up the walls of the dish probably about 1/4 of an inch off of the agar. The white mystery fuzz looked like it stopped growing as well about a 1/4 inch from the edge of the plate. I made 8 transfers in a SAB to new agar plates and it has been about two days and mycelium is starting to grow aggressively with no signs of contams this far. Fingers crossed...
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