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Offlinestilltrying
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Thin mycellium or mold? * 1
    #18788133 - 09/02/13 03:04 AM (10 years, 4 months ago)

Hi all,

I have injected LC (made from store bought King Oyster) into a few jars and am  bit confused by the result. Previously, i had thrown pieces of mushroom into grain jars and what I got was very thick white fluffy growth radiating outwards from the substrate(like rays coming out of each grain).

In the meantime I had a couple LCs growing, made with honey, with a piece of king oyster tissue injected with a 14g needle. One turned out to be contamed, producing green when injected into grain, while the other produced white myc when tested on grain. I now innoced several jars with it, but the growth looks rather unfamiliar. The jars I have made are with brown rice  or wheat berries, soaked and pressure cooked as per the recomendations of this forum (15 PSI for 90min, often more, after a 12h soak with water+coffee+gypsum, while rice is boiled 'al dente' and then pc-ed for the same time)


The brown rice jars are producing growth that looks very thick and white just like the myc growing on the mushrooms themselves when bought off the shelf, or the growth produced when putting a piece of the mushroom into grain.

I also injected the LC into a few jars of coir/brf/coffee 60/20/20. Growth looks normal, radiating from the injection points.

However, on wheat berries the growth looks very dry, thin.

Initially I thought my berries are too dry, so I injected water(sterile) into one such jar, 10ml for 200ml of grains. The water did not touch the berries, but rather ran off to the bottom without wetting them, as if they had been waxed. THey were covered in a very fine network of myc, which I assumed to be cobweb mold, as it was spread everywhere and not looking like normal myc. I threw that jar out and made some more, but these also seem to grow very slow.

Jar in pic below had reached 10% colonisation by what seemed to be healthy thick fluffy myc, so i gave it a shake, and now, 3 days later there seems to be a very thin, hardl visible network covering all of the grains. Are my grains too dry, making the myc thin? Do I have too much GE? or is it cobweb mold?




and a pic of the coir mix, showing what looks to be normal pleurotus myc:


Another indicator of what could be wrong is that the temp dropped over the last week to 20 celsius from 22 celsius before


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InvisibleOeric McKenna
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Re: Thin mycellium or mold? [Re: stilltrying]
    #18788276 - 09/02/13 04:22 AM (10 years, 4 months ago)

Its really hard to say with these pictures.  You really have to verify if the culture is clean first. 
Honestly, judging by your methods, it is unlikely.  The one you're saying looks normal, unfortunately doesn't look too normal. There are different tones in contrast.

Some things to really take to heart about liquid innoculants:
- the SLIGHTEST bit of contamination will grow vigorously
-  usually can't see the contamination until after inoculation

  Now, these are serious setbacks, but I do favor liquid culture, because if it is pure, it has the highest success rate at inoculation if an injection port is used.  Its just that intensive measures must be taken to reach that mark.
    Sticking a chunk of mushroom into a nutrient solution is a low probability idea. It could work, but its a gamble. So this gamble is a bad foundation for your work. 

What are those lids?

  Send me some PMs if you want, and I'll help you get on a good path


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Offlineeddieblake
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Re: Thin mycellium or mold? [Re: Oeric McKenna]
    #18788496 - 09/02/13 06:41 AM (10 years, 4 months ago)

In my experience, i think i have had every type of contam there is.  lol  the myc can look different depending on grain or even water content.  I have had yellow oyster myc look like your first pic, in one jar, and look really thick in a different attempt.  Pink oyster myc looks like cobweb to me.  the king myc i have tried always looked thick. so, i would be patient and see where it goes.


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Offlinestilltrying
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Re: Thin mycellium or mold? [Re: eddieblake]
    #18788863 - 09/02/13 09:35 AM (10 years, 4 months ago)

Thank you. Looking at other jars in a similar state I am starting to believe this is just very thin myc, possibly due to dry grains. I followed the same hydration procedure as usual with this batch, but it seems to have come out too dry. I even have clumps of myc from the LC injection sitting there and doing next to nothing in another jar (facepalm). I find it's very unusual though.

Thank you for letting me know that it CAN take the look of cobweb. Will wait and will report with progress. Will inject an extra 10% water content in half the jars, since I am used to seeing some condensation but right now there is none.


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Offlinedrake89
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Re: Thin mycellium or mold? [Re: stilltrying]
    #18789869 - 09/02/13 01:14 PM (10 years, 4 months ago)

my two cents:

your grains are not too dry, they look fine.  they would not absorb extra water because they already are hydrated.  They are also much more nutrient rich than your coir mix, which is why the contams took hold first, and your coir jar is doing fine.  Your grain spawn is bunk, kind oyster myc is not that think IME.  sorry.  I have composted well over 250 pound of grain spawn, it sucks, live and learn.  I would steer clear of liquid culture unless you have a flow hood.  even then it can be dicey unless you have VERY good sterile technique and clean cultures to start with.  Pleurotus species can over run most contams, but in bulk substrate, not so much grain spawn.


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Offlinestilltrying
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Re: Thin mycellium or mold? [Re: drake89]
    #18814029 - 09/08/13 04:04 AM (10 years, 4 months ago)

They were fine. just really thin mycellium. It covers the surface in a very thin whitish fuzz that looks more like cobweb, and then thickens. Will post pics later.


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Offlinestilltrying
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Re: Thin mycellium or mold? [Re: stilltrying]
    #18887330 - 09/24/13 11:29 PM (10 years, 4 months ago)

I spawned some of that grain to coir. colonised well. Scratched surface and placed outside on window sil (12-18 celsius)

4 days later:

5 days later:


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Offlinestilltrying
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Re: Thin mycellium or mold? [Re: stilltrying]
    #18925799 - 10/03/13 01:08 PM (10 years, 3 months ago)



the jar


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Offlinesporeguy
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Re: Thin mycellium or mold? [Re: stilltrying]
    #21284903 - 02/16/15 06:50 PM (8 years, 11 months ago)

Quote:

stilltrying said:
They were fine. just really thin mycellium. It covers the surface in a very thin whitish fuzz that looks more like cobweb, and then thickens. Will post pics later.




would love pics. Im about 10 days and 2 shake/rolls into colonising my wheat berries and the mycellium looks like your first pictures- no large radial growth patterns like im used to in BRF - almsot like individual berries or clumps of 3-5 berries are wrapped in a thin layer of whiting fuzz.

im almost incluned to think the spawn is a bit too dry (the berries at the top of the jar are slightly browner and dehydrated), or is it too soon?

should i see the mycellium coating of the grains thicken in the next few days, or will it remain kinda thin and whispy?


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