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Guest F
Stranger

Registered: 01/26/13
Posts: 458
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Monotub question
#18784629 - 09/01/13 10:01 AM (10 years, 4 months ago) |
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So with my monotub right now, I can see 4 pins, 2 on the surface and 2 on top. But there are a few small spots where it isn't colonized. My question is can I put it into fruiting conditions or do I need to wait for full colonization?
-------------------- All cultivation posts are what I believe to be accurate. If I give ANY misinformation, please do not hesitate to correct me.
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Trippy_Penguin



Registered: 03/18/12
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Re: Monotub question [Re: Guest F]
#18784690 - 09/01/13 10:27 AM (10 years, 4 months ago) |
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Do not fruit until 100%. Fruits before full colonization can mean contamination, but keep an eye on it.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Monotub question [Re: Guest F]
#18784691 - 09/01/13 10:27 AM (10 years, 4 months ago) |
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Yes, if it is pinning, it is telling you it needs fresh air and can find no more food.
Are you using coir?
Sometimes coir will dry out a little and not colonize completely, esp if you use the top layer over the grains.
It's also possible there is a bacteria problem and the mycelium is no longer able to colonize the rest of the substrate.
A picture would help. But yes, if it is pinning, it is ready to fruit.
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Trippy_Penguin



Registered: 03/18/12
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Frank - Would it be unwise to keep an eye on it for another day and see if those areas close up? I definitely agree that coir can end up with some uncolonized spots, but is there any chance it will finish up if given some time? (Need this info to store away in my vault of knowledge)
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Midnight Cyclone
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Registered: 05/29/13
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I've had coir subs colonize like 85% in 2 weeks and I try to wait until 100% but even at 3 weeks they were not 100%.
I blame this on bucket tek pasteurization giving me incorrect moisture content.
Like Frank said, fruit the tub and take extra care when doing work with it as that uncolonized coir is essentially a breeding ground for whatever lands first and grows fastest. By this I mean take necessary sterile procedure while working.
Good luck, and post a picture. They're worth so much!
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
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Quote:
Midnight Cyclone said: I've had coir subs colonize like 85% in 2 weeks and I try to wait until 100% but even at 3 weeks they were not 100%.
I blame this on bucket tek pasteurization giving me incorrect moisture content.
I think it would be safer to assume you did not mix the grains and the sub evenly enough.
Just my 2 cents tho....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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IMO if it is pinning, it is done colonizing and ready for fresh air.
Your mycelium is telling you it has found all the "food" it can for whatever reason.
Those spots are usually just fine with coir but can be indicative of bacteria.
If that is the case, the contam is already present and FAE will only help. If anything, it will help to "knock back" the bacteria (which loves stale air) and give the mycelium the boost it needs.
Quote:
Midnight Cyclone said: Like Frank said [...] take extra care when doing work with it as that uncolonized coir is essentially a breeding ground for whatever lands first and grows fastest. By this I mean take necessary sterile procedure while working.
That is not what I said 
This is pasteurized coir, not sterile media.
You spawn in open air, so you can assume contams spores and bacteria are already mixed in.
Since you pasteurized and used fully colonized grain spawn, this is contam-resistant enough.
The "uncolonized" coir is either too wet/dry or it is already colonized by something you can't see, like bacteria
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Midnight Cyclone
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Re: Monotub question [Re: PussyFart]
#18784861 - 09/01/13 11:10 AM (10 years, 4 months ago) |
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Quote:
Notahacker420 said:
Quote:
Midnight Cyclone said: I've had coir subs colonize like 85% in 2 weeks and I try to wait until 100% but even at 3 weeks they were not 100%.
I blame this on bucket tek pasteurization giving me incorrect moisture content.
I think it would be safer to assume you did not mix the grains and the sub evenly enough.
Just my 2 cents tho....
The subs were noticeably more wet than usual when I set into colonization.
There was nothing I could do though, risk contamination trying to squeeze water out of the substrate or hope it evaporates off and goes well?
I went with the latter.
My substrate and grains are always mixed thoroughly. I've actually been wondering recently whether I'm mixing them up too much, and if smaller chunks of mycelium might be better than breaking WBS cakes into essentially individual grains. Whether it would help with recover time or not. But that's another discussion for another time.
I should have said that differently.
Quote:
Like Frank said, fruit the tub.
Just take extra care when doing work with it as that uncolonized coir is essentially a breeding ground for whatever lands first and grows fastest. By this I mean take necessary sterile procedure while working.
I was agreeing with you to fruit the tub, and disagreeing with petersodm who said to let it colonize more. Not trying to put words in your mouth about the sterile procedure he needed to take. That was just my 2 pennies.
Edited by Midnight Cyclone (09/01/13 11:14 AM)
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
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Last seen: 17 days, 14 hours
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Breaking them up into individual inoculation points is the best.
The mycellium grows outward in a 3D spherical pattern.
You would want to take full advantage of every possible inoculation point.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Guest F
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Re: Monotub question [Re: PussyFart]
#18785623 - 09/01/13 02:42 PM (10 years, 4 months ago) |
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Frank: yes I am using coir and the spots that aren't colonized are on the bottom - didn't use liners this grow. The top is fully colonized and so are the sides. Would post a picture but it'll be difficult to take a picture of the bottom of the tub. Probably just going to fruit it. Thanks for all the info guise.
-------------------- All cultivation posts are what I believe to be accurate. If I give ANY misinformation, please do not hesitate to correct me.
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Guest F
Stranger

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Re: Monotub question [Re: Guest F]
#18785701 - 09/01/13 03:04 PM (10 years, 4 months ago) |
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Quick question: About how long after introducing to fruiting conditions will I start to see more pins?
-------------------- All cultivation posts are what I believe to be accurate. If I give ANY misinformation, please do not hesitate to correct me.
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Trippy_Penguin



Registered: 03/18/12
Posts: 624
Last seen: 3 years, 5 months
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Re: Monotub question [Re: Guest F]
#18785717 - 09/01/13 03:08 PM (10 years, 4 months ago) |
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Can be a couple of days to a week or two. Multispore is very unpredictable.
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