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Offlinesocraticd
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Registered: 08/06/13
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Last seen: 3 years, 19 days
First oyster spawn. Contam'd or good mycelium?
    #18784518 - 09/01/13 09:17 AM (10 years, 4 months ago)

SO, my first attempt at WBS spawn was a little off.  The jars were really wet, and had some standing water in them, which i was able to SOMEWHAT alleviate by turning them on their side a quarter turn a day for a few days.  I definitely have a few zones of stalled growth, and POSSIBLY some bacteria in the bottoms of the jars, as they look discolored in those places and no real growth that looks like mycelium.

This was done with a syringe I made from a blue oyster spore print I took.  I have a new LC syringe of blues on the way, but I figured a MS experiment wouldn't hurt anything, and I can clone anything that might fruit from these)

The question I have, having never grown on grain in a jar, is how fluffy should the top look?  I originally assumed these were contam'd since the top was fluffy, almost looking like cotton on top of the WBS (I figured some sort of white mold?), but since it looks like mycelium further down, I'm thinking these might be colonizing?  Albeit slowly? 

Wondering if the extremely wet spawn material slowed down colonization time?  These were shot up on 8/10 with probably 4cc each of really weak solution that came from spore prints i could BARELY see..

Thoughts?  Just want to see if I can verify what I think I'm looking at.  Sorry the pictures aren't awesome, seems touch to get a good pick through the jar.  It looks like a fluffy layer of cotton on top.  Is that because the humidity is so high (note condensation)?



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Offlinekrypto2000
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Registered: 12/05/06
Posts: 11,579
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Re: First oyster spawn. Contam'd or good mycelium? [Re: socraticd]
    #18784556 - 09/01/13 09:36 AM (10 years, 4 months ago)

I'm thinking bacterial contam, that almost always happens when they're wet/mushy looking, but you might want to leave them alone and see if they recover. How long has it been?


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OfflineForresterM
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Registered: 02/05/13
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Re: First oyster spawn. Contam'd or good mycelium? [Re: krypto2000]
    #18784825 - 09/01/13 11:01 AM (10 years, 4 months ago)

Yeah that looks like mycelium on the top, although if it was too wet like you say it probably is fighting some bacteria. 

I had a jar of turkey tail spawn recently that had the same problem, some areas not colonized yet and wet looking.  Pretty sure there was bacteria.  What I did was shake it up thoroughly, so it distributed the water/bacteria evenly and the mycelium recovered perfectly with no signs of contamination.  Oyster mycelium loves to eat bacteria too, so you might give it a shot, sometimes it wins.


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