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BittrBuffalo
Deaconica

Registered: 05/19/13
Posts: 1,729
Loc: Church of the SubGenus
Last seen: 3 years, 5 months
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Most common reason for contam?
#18781470 - 08/31/13 02:03 PM (10 years, 5 months ago) |
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There are SOO many variables that can go wrong and cause contam in your work, but what's the most common source? Insufficient substrate sterilization? Contamination during inoculation? Preparing the inoculant?
I just started 18 BRF jars and 4 of them ended up with blue-green mold contam. The jars were homemade, but not all were sterilized in the same pot. I think this is the source of the contam, but I'd like to know the most likely culprit.
-------------------- Disclaimer: This post is a work of fiction, provided for entertainment purposes only. Any resemblance to actual persons or events, past or present, is strictly coincidental. All celebrity voices are impersonated. If you begin your ID request with, "I just ate a bunch of these mushrooms…should I not have done that?" I'm just gonna sit back and watch Darwin at work.
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Guest F
Stranger

Registered: 01/26/13
Posts: 458
Last seen: 7 years, 8 months
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Re: Most common reason for contam? [Re: BittrBuffalo] 1
#18781478 - 08/31/13 02:07 PM (10 years, 5 months ago) |
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You either improperly sterilized(not long enough) or poor inoculation procedures. Always flame sterilize and never wipe off the needle after flaming.
-------------------- All cultivation posts are what I believe to be accurate. If I give ANY misinformation, please do not hesitate to correct me.
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: Most common reason for contam? [Re: Guest F] 1
#18782069 - 08/31/13 05:05 PM (10 years, 5 months ago) |
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Improper sterile procedure is the most common reason for getting a contaminant. If you inoculated all the jars with same syringe and 4 of them contam while the others didn't, I'd suspect improper flaming of the needle between jars or a bad syringe. Remember, no print or MS syringe is 100% sterile, because the mushrooms are fruited in open air.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,865
Last seen: 1 day, 8 hours
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IME contaminated spores. When I started using agar my contamination rate dropped to 0% for most batches overnight.
I should also mention I noticed a sigfnificant improvement before that switching to dry spore inoculations instead syringes. With syringes I was losing entire batches sometimes.
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Edited by Kizzle (08/31/13 06:30 PM)
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annoyed
Stranger

Registered: 04/12/20
Posts: 33
Last seen: 6 months, 12 hours
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Re: Most common reason for contam? [Re: BittrBuffalo]
#27260569 - 03/19/21 02:25 PM (2 years, 10 months ago) |
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I've seen someone colonize about fifty jars with just a single contamination.
None of the jars were pressure cooked.
The jars were wide-mouth with four holes in the lid for air exchange and injection.
No cotton plugged them, no micro pore tape. Instead, aluminum foil was placed on the jars.
The jars were cleaned before injection by steaming them in a normal stockpot for three hours.
One jar was steamed for just thirty minutes and did not contaminate with a substrate of flour with extra chaff mixed in. (2:1:1 chaff, flour, water, in that order.)
Aside from that one jar, normal PF Tek was used.
All worked fine with no pressure canner, auto clave, etc.
Thus I conclude the most common cause of contamination is a dysgenic couple of spores taking too long to colonize.
I advise everyone to ignore all the rules set up by these amateur growers larping like actual scientists.
They muddy the water to make it seem deep. -Friedrich Nietzsche
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Professor X
School for the Gifted



Registered: 04/18/19
Posts: 2,719
Last seen: 2 years, 4 months
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Re: Most common reason for contam? [Re: annoyed]
#27260594 - 03/19/21 02:36 PM (2 years, 10 months ago) |
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I pressure cooked 21 jars of BRF at 12 PSI for 60 minutes and inoculated them all with spore syringes in front of a flowhood. 3 grew mycelium but never fruited because they were so bacteria laden, 18 grew mold in various amounts of time. This is with standard PF Tek lids with 3x MP tape. So yeah, disregard whatever you feel and come tell us why you think the spores ooked you in the dooker.
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annoyed
Stranger

Registered: 04/12/20
Posts: 33
Last seen: 6 months, 12 hours
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Re: Most common reason for contam? [Re: Professor X]
#27260608 - 03/19/21 02:44 PM (2 years, 10 months ago) |
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Quote:
Professor X said: I pressure cooked 21 jars of BRF at 12 PSI for 60 minutes and inoculated them all with spore syringes in front of a flowhood. 3 grew mycelium but never fruited because they were so bacteria laden, 18 grew mold in various amounts of time. This is with standard PF Tek lids with 3x MP tape. So yeah, disregard whatever you feel and come tell us why you think the spores ooked you in the dooker.
You elucidate how you failed using standard amateur procedures, and have concluded I have been ooked with no failures.
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Tight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 2,116
Last seen: 5 months, 1 day
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Re: Most common reason for contam? [Re: annoyed] 2
#27260614 - 03/19/21 02:48 PM (2 years, 10 months ago) |
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Quote:
annoyed said: I've seen someone colonize about fifty jars with just a single contamination.
None of the jars were pressure cooked.
The jars were wide-mouth with four holes in the lid for air exchange and injection.
No cotton plugged them, no micro pore tape. Instead, aluminum foil was placed on the jars.
The jars were cleaned before injection by steaming them in a normal stockpot for three hours.
One jar was steamed for just thirty minutes and did not contaminate with a substrate of flour with extra chaff mixed in. (2:1:1 chaff, flour, water, in that order.)
Aside from that one jar, normal PF Tek was used.
All worked fine with no pressure canner, auto clave, etc.
Thus I conclude the most common cause of contamination is a dysgenic couple of spores taking too long to colonize.
I advise everyone to ignore all the rules set up by these amateur growers larping like actual scientists.
They muddy the water to make it seem deep. -Friedrich Nietzsche
You bumped a 7.5 year old thread in order to give people really bad advice. Congratulations.
-------------------- "it's all a joke between mom contractions and coffin fittings" The most useful tool for noobs
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annoyed
Stranger

Registered: 04/12/20
Posts: 33
Last seen: 6 months, 12 hours
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Quote:
Tight Lunchbox said:
Quote:
annoyed said: I've seen someone colonize about fifty jars with just a single contamination.
None of the jars were pressure cooked.
The jars were wide-mouth with four holes in the lid for air exchange and injection.
No cotton plugged them, no micro pore tape. Instead, aluminum foil was placed on the jars.
The jars were cleaned before injection by steaming them in a normal stockpot for three hours.
One jar was steamed for just thirty minutes and did not contaminate with a substrate of flour with extra chaff mixed in. (2:1:1 chaff, flour, water, in that order.)
Aside from that one jar, normal PF Tek was used.
All worked fine with no pressure canner, auto clave, etc.
Thus I conclude the most common cause of contamination is a dysgenic couple of spores taking too long to colonize.
I advise everyone to ignore all the rules set up by these amateur growers larping like actual scientists.
They muddy the water to make it seem deep. -Friedrich Nietzsche
You bumped a 7.5 year old thread in order to give people really bad advice. Congratulations.
I gave childish advice backed with experience. Boomer.
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Tight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 2,116
Last seen: 5 months, 1 day
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Re: Most common reason for contam? [Re: annoyed]
#27260641 - 03/19/21 03:07 PM (2 years, 10 months ago) |
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Your experience that brf jars can be steam sterilized? That isn't some sort of revelation. People do it all the time. It's the pf tek.
-------------------- "it's all a joke between mom contractions and coffin fittings" The most useful tool for noobs
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annoyed
Stranger

Registered: 04/12/20
Posts: 33
Last seen: 6 months, 12 hours
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Quote:
Tight Lunchbox said: Your experience that brf jars can be steam sterilized? That isn't some sort of revelation. People do it all the time. It's the pf tek.
You've exposed yourself as either dumb or egotistical by saying I didn't use 50 PF Tek jars, with one jar of regular flour and chaff that was steamed as well with no contamination.
What you're experiencing is true childishness, like I've attacked your dogma taught by your very own mother.
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Tight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 2,116
Last seen: 5 months, 1 day
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Re: Most common reason for contam? [Re: annoyed]
#27260669 - 03/19/21 03:26 PM (2 years, 10 months ago) |
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I never said you didn't use 50 pf tek jars...
-------------------- "it's all a joke between mom contractions and coffin fittings" The most useful tool for noobs
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annoyed
Stranger

Registered: 04/12/20
Posts: 33
Last seen: 6 months, 12 hours
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Quote:
Tight Lunchbox said: I never said you didn't use 50 pf tek jars...
You aren't making a clear point for why people can't be satisfied with PF Tek, and I've yet to see regular flour or other substrates fail with just steaming. '
Go to church if you need to be part of a religious community.
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Tight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 2,116
Last seen: 5 months, 1 day
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Re: Most common reason for contam? [Re: annoyed]
#27261465 - 03/20/21 07:26 AM (2 years, 10 months ago) |
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I was never satisfied with pf tek because I like growing a shit ton of mushrooms. That's why I use grains. Grow however you want to grow. Be sure to keep us informed with your results.
The general consensus on the best practices for growing isn't gospel and it has certainly changed over time. The advice of TC's and others with experience is pretty solid because they've proven what does and doesn't work. To come in here and bump a 7.5 year old thread and say that legit growers in this community are "amateur growers larping like actual scientists" is an obvious attempt at trolling, so I'm clearly wasting my time by typing all of this out. Good luck man.
-------------------- "it's all a joke between mom contractions and coffin fittings" The most useful tool for noobs
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annoyed
Stranger

Registered: 04/12/20
Posts: 33
Last seen: 6 months, 12 hours
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Quote:
Tight Lunchbox said: I was never satisfied with pf tek because I like growing a shit ton of mushrooms. That's why I use grains. Grow however you want to grow. Be sure to keep us informed with your results.
The general consensus on the best practices for growing isn't gospel and it has certainly changed over time. The advice of TC's and others with experience is pretty solid because they've proven what does and doesn't work. To come in here and bump a 7.5 year old thread and say that legit growers in this community are "amateur growers larping like actual scientists" is an obvious attempt at trolling, so I'm clearly wasting my time by typing all of this out. Good luck man.
All communities are toxic to me with their zealotry that's disguised as legitimate experience when it's all just egotistical ways to find meaning in their boring lives. what I said was for the few who have doubts about what is commonly advised as necessary, using "logic" but from a starting point of untruths. The result is a system of lies, illogical lies, unpractical wastes of time and effort. The few who want to hear doubt, I have provided them with it.
And as is common practice on all forums, old posts get revived if a new thought wants to be added.
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annoyed
Stranger

Registered: 04/12/20
Posts: 33
Last seen: 6 months, 12 hours
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Re: Most common reason for contam? [Re: annoyed]
#27261575 - 03/20/21 09:17 AM (2 years, 10 months ago) |
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they use "logic"* and make systems of logical lies
by the way, you still haven't proven PF Tek is the only magical tek that doesn't need 3 hours of pressure cooking to "sterilize it" from mold spores. Would you boil your syringes for even 10 minutes before using them? SILLY. Most of what needs tobe killed gets killed by boiling, strong spores grow quickly, and those spores are the ones we want anyway. Doing large jars is silly, anyway, as the point is to select jars that grow quickest. You cant perpetuate a specific culture, you always need to colonize with new spores to prevent inbreeding.
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Tight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 2,116
Last seen: 5 months, 1 day
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Re: Most common reason for contam? [Re: annoyed]
#27261603 - 03/20/21 09:33 AM (2 years, 10 months ago) |
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If you have a better way of doing it then share it with the community. Let's see it. Show us a better way to grow pounds than what has already been established.
-------------------- "it's all a joke between mom contractions and coffin fittings" The most useful tool for noobs
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Benson
The Kidd ⭐


Registered: 09/29/20
Posts: 837
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Re: Most common reason for contam? [Re: annoyed]
#27261642 - 03/20/21 10:06 AM (2 years, 10 months ago) |
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Quote:
annoyed said: All communities are toxic to me with their zealotry that's disguised as legitimate experience when it's all just egotistical ways to find meaning in their boring lives.
Yo, how about you get the fuck out then?
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smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,882
Last seen: 5 hours, 34 minutes
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Quote:
TranscendingLife said: Improper sterile procedure is the most common reason for getting a contaminant. If you inoculated all the jars with same syringe and 4 of them contam while the others didn't, I'd suspect improper flaming of the needle between jars or a bad syringe. Remember, no print or MS syringe is 100% sterile, because the mushrooms are fruited in open air.
Do most TCs really flame between each jar
Does everyone else do this too?
-------------------- Willpower is the one true virtue
  
Edited by smalltalk_canceled (03/20/21 10:10 AM)
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annoyed
Stranger

Registered: 04/12/20
Posts: 33
Last seen: 6 months, 12 hours
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Quote:
Tight Lunchbox said: If you have a better way of doing it then share it with the community. Let's see it. Show us a better way to grow pounds than what has already been established.
I could never advise a better way to grow mushrooms than what a grower has learned intuitively, with a few guiding principles.
To every doubtful grower who wants my opinion, which isn't opinionated at all: 1. Do what is expedient for you, never seek approval 2. Use good logic and believe in it above all else 3. Hide your knowledge and science, show others only your art 4. Ignore every manmade rule
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