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InvisibleFrankHorrigan
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Speed of colonization: the advantages of dunking colonized grain (agar pictures) * 1
    #18781503 - 08/31/13 02:17 PM (10 years, 4 months ago)

So I took a bag of grains that had been colonized with an isolate.

I removed one grain before dunking the rest.

I also took a dunked grain of the same size.

I put the two on agar to see just how much the mycelium benefits from being rehydrated before spawning to bulk.

Here is the timeline, today I realized this would be a good thread of its own:

8/27 (24 hours after being placed on the dish):


8/29


8/30


8/31


Here is the not-dunked side vs dunked:


9/1






I know which one I want mixed into my substrate :derfase:

:cheers:


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Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Edited by FrankHorrigan (09/01/13 11:39 AM)


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OfflineTrippy_Penguin
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Re: Speed of colonization: the advantages of dunking colonized grain [Re: FrankHorrigan]
    #18781510 - 08/31/13 02:21 PM (10 years, 4 months ago)

Results you can't argue against. Awesome Frank.

:cookiemonster:

I started dunking my grains about a month ago and the results have been very pleasing.


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InvisiblebodhisattaMDiscordReddit
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Re: Speed of colonization: the advantages of dunking colonized grain [Re: Trippy_Penguin]
    #18781526 - 08/31/13 02:25 PM (10 years, 4 months ago)

Let us know if you see a difference in actual colonization times with your subs, but from this alone I'm dunking for myself to try it

Edit

Colonization times for bulk substrate decrease, sometimes by as much as 50%+.
Fruits are thicker and healthier.
Yields are improved.
-FH

Well that answers my question


Edited by Trusted cuItivator (08/31/13 02:27 PM)


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Invisiblerefried

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Re: Speed of colonization: the advantages of dunking colonized grain [Re: bodhisatta]
    #18781661 - 08/31/13 03:13 PM (10 years, 4 months ago)

I just dunked my grains and used the water to get my compost, which was a bit dry, to field capacity.  Do you think using the dunk water will aid colonization?


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InvisiblebodhisattaMDiscordReddit
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Re: Speed of colonization: the advantages of dunking colonized grain [Re: refried]
    #18781667 - 08/31/13 03:16 PM (10 years, 4 months ago)

Dunk water + agar ? nutritious enough?


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Offlinemushrume man
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Re: Speed of colonization: the advantages of dunking colonized grain [Re: bodhisatta]
    #18781670 - 08/31/13 03:16 PM (10 years, 4 months ago)

Those are great results! Im dunking for sure now. But just to be scientific about can you repeat it a couple times?

I noticed that the myc differences look like the difference in my two MS bags of PE. Maybe one is less hydrated? I got them mail order.

I did see you used an isolate. But I have read there can be multiple varieties if its an isolate from MS.


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InvisibleFrankHorrigan
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Re: Speed of colonization: the advantages of dunking colonized grain [Re: bodhisatta]
    #18781732 - 08/31/13 03:38 PM (10 years, 4 months ago)

Quote:

mushrume man said:
I did see you used an isolate. But I have read there can be multiple varieties if its an isolate from MS.




I'm not sure I follow.

This is an isolate, there is no "variety" whatsoever. It is a single sector, one of about 50 isolates I keep in slants.

It doesn't really get more controlled than this :shrug:

If you are using MS, you can expect everything to look different every time, from mycelium to fruits.

Quote:

refried said:
I just dunked my grains and used the water to get my compost, which was a bit dry, to field capacity.  Do you think using the dunk water will aid colonization?




It sure as hell won't hurt to try :shrug:

Pasteurize it properly before you spawn to it.

Quote:

bodhisatta said:
Dunk water + agar ? nutritious enough?




This would be a question for my agar journal or the grain dunk thread :thumbup:

Quote:

petersodm said:
I started dunking my grains about a month ago and the results have been very pleasing.




:highfive:


--------------------

Yes, you can bump my old threads with a question.
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You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
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Invisiblerefried

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Re: Speed of colonization: the advantages of dunking colonized grain [Re: FrankHorrigan]
    #18781885 - 08/31/13 04:18 PM (10 years, 4 months ago)

Hopefully it was still okay from the last time I used it, about a month ago.  Bought 10lbs from a sponsor and kept it sealed in a bag inside the fridge.  Crossing my fingers.  :laugh:


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InvisibleFrankHorrigan
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Re: Speed of colonization: the advantages of dunking colonized grain [Re: refried]
    #18782027 - 08/31/13 04:54 PM (10 years, 4 months ago)

Re pasteurize it for sure :thumbup:


--------------------

Yes, you can bump my old threads with a question.
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You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
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InvisibleFrankHorrigan
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Re: Speed of colonization: the advantages of dunking colonized grain (agar pictures) [Re: FrankHorrigan]
    #18784958 - 09/01/13 11:40 AM (10 years, 4 months ago)

Quote:

FrankHorrigan said:
9/1








This about settles it for me :shrug:

Maybe I'll do this with some other species as well.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
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OfflineTrippy_Penguin
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Re: Speed of colonization: the advantages of dunking colonized grain (agar pictures) [Re: FrankHorrigan]
    #18785061 - 09/01/13 12:14 PM (10 years, 4 months ago)

Since dunking isn't done with sterile procedure, is it a contam risk to use dunked grain for my future agar projects? It seems you pulled it off and that growth is impressive. I've always used non-dunked grains for agar while doing g2g.


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InvisibleFrankHorrigan
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Re: Speed of colonization: the advantages of dunking colonized grain (agar pictures) [Re: Trippy_Penguin]
    #18785079 - 09/01/13 12:19 PM (10 years, 4 months ago)

Are you asking if you can take a grain from your drained spawn jar and put it to agar without much sterile procedure?

Yes, you can, it's very easy.

The mycelium is recovering so fast it will outrun any contams. The dunked grain even rolled around a bit on the petri dish, but it colonized before anything else had a chance.

I wear gloves and take a clean, unopened ziplock bag.

I take the piece(s) of grain out of the pile and put it into the bag.

I seal the bag quickly and spawn the tub.

Then I go to my "lab" with the ziplock, wipe down my gloves, put on tyvek sleeves, and transfer the grain(s) by hand to the petri dish(es).

You will get some really aggressive growth. Make sure to take at least one transfer before using it to inoculate more grains.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Re: Speed of colonization: the advantages of dunking colonized grain (agar pictures) [Re: FrankHorrigan]
    #18785094 - 09/01/13 12:26 PM (10 years, 4 months ago)

That's exactly what I was wondering. Thank you.


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OfflinePandax
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Re: Speed of colonization: the advantages of dunking colonized grain (agar pictures) [Re: FrankHorrigan]
    #18785101 - 09/01/13 12:28 PM (10 years, 4 months ago)

So if one was to use quart jars for a bulk spawn, would you just fill the fully colonized jar up and leave it overnight. Drain and spoon out the grain into the bulk substrate?

Edit: Nvm found the link with the info I needed. I will be using this method next go around.


Edited by Pandax (09/01/13 12:40 PM)


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InvisibleFrankHorrigan
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Re: Speed of colonization: the advantages of dunking colonized grain (agar pictures) [Re: Pandax]
    #18785112 - 09/01/13 12:32 PM (10 years, 4 months ago)

Quote:

Pandax said:
So if one was to use quart jars for a bulk spawn, would you just fill the fully colonized jar up and leave it overnight. Drain and spoon out the grain into the bulk substrate?




The process is described in detail here:

Quote:

FrankHorrigan said:
How to do it the quick way:

I take a roll of fiberglass screen (the kind used in window screens) and cut a piece to fit around the top of a five gallon bucket.

Grab the five gallon bucket and clean it out. 

Break up your spawn jars and give each a good smell to make sure it is clean.

Dump the contents of each clean jar into the bucket and fill with enough cold water (not distilled) to cover the grains.


Attach the house screen with a rubber band and let it soak for 10 to 20 minutes.


When the time is up, secure the screen with your hand around the edges and tilt until the water pours out. It will drain very quickly, tapering down to a small stream after 10-20 seconds.


How to do it the other way:

Fill each jar with cold water (not distilled). Let it soak for 10 to 20 minutes.


Drain by placing the lid over the top and leaving a gap between the lip of the jar and your lid. Drain it well. The grains will be quite wet at this point.






Hope this helps :thumbup:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflinePandax
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Re: Speed of colonization: the advantages of dunking colonized grain (agar pictures) [Re: FrankHorrigan]
    #18785145 - 09/01/13 12:42 PM (10 years, 4 months ago)

Haha found that thread right before you responded, thanks!


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Invisiblej3rk0ff
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Re: Speed of colonization: the advantages of dunking colonized grain (agar pictures) [Re: Pandax]
    #18867413 - 09/20/13 12:14 PM (10 years, 4 months ago)

sweet want to try this out with a isolate i have from a good ol' friend of mine.

would the mycelium ever weaken or degrade after so many transfers?


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OfflineYuri.Pono
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Re: Speed of colonization: the advantages of dunking colonized grain (agar pictures) [Re: j3rk0ff]
    #19407572 - 01/12/14 09:52 AM (10 years, 19 days ago)

:cookiemonster: BUMP for learning great stuff here


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