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JoshRoomar
Lurker


Registered: 01/18/13
Posts: 24
Last seen: 10 years, 3 months
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Is my syringe empty??
#18780881 - 08/31/13 10:48 AM (10 years, 4 months ago) |
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It's been a while since my last post. I've been experimenting with a few teks and you guys really helped me a lot just from reading your day to day experiences, so my sincere gratitude to all of you smart shroomheads! Now to my question. I've bought 10ml of sclerotia producer, Atlantis strain to be precise, from a trusted vendor. Now I say trusted, because it's one of the biggest out there and as far as my experience goes, I would assume I can "trust" this vendor. Now from the first look the vial looked empty, but I didn't pay much attention to it, as I know the spores are really small. So I knocked two rye jars and four LME agar dishes and hoped for the best. More than one month and nothing. Not even sign of contamination. So I tossed it away with the result, that there is just no spores in the solution. It never happened to me before and as I don't have microscope, there is no other way to determine if it's "empty" or not. Or is it?? Did this happen to you before?? And I didn't add coffee to it, might that be the problem (I would say no, but never did sclerotia before)?? Again thx for replies
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Trippy_Penguin



Registered: 03/18/12
Posts: 624
Last seen: 3 years, 5 months
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Re: Is my syringe empty?? [Re: JoshRoomar]
#18780899 - 08/31/13 10:55 AM (10 years, 4 months ago) |
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Did you shake it well prior to using it?
If you are going through a good vendor, you should email them and let them know the syringe was no good. Lots of reputable vendors will send you a new one if the original was bad.
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The Psyentist
Figment of my Imagination


Registered: 07/30/13
Posts: 82
Loc: Central Florida
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Re: Is my syringe empty?? [Re: JoshRoomar]
#18780907 - 08/31/13 10:57 AM (10 years, 4 months ago) |
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I'm not saying it was bunk. But, I only trust sponsors of this website. You don't have to see spores for them to be in there. So without a microscope or results, it's hard to say.
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FruitOfLife
Professional Package Handler


Registered: 05/21/12
Posts: 4,832
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Quote:
petersodm said: Did you shake it well prior to using it?
If you are going through a good vendor, you should email them and let them know the syringe was no good. Lots of reputable vendors will send you a new one if the original was bad.
word it as "spores not viable" Dont say things like mycelium wont grow or anything related to growing. You dont want to get a vendor in trouble
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The Psyentist
Figment of my Imagination


Registered: 07/30/13
Posts: 82
Loc: Central Florida
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also
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Trippy_Penguin



Registered: 03/18/12
Posts: 624
Last seen: 3 years, 5 months
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Quote:
FruitOfLife said:
word it as "spores not viable" Dont say things like mycelium wont grow or anything related to growing.
Good call on that.
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mastercultivator
Master Cultivator



Registered: 08/27/13
Posts: 653
Last seen: 7 months, 2 days
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Re: Is my syringe empty?? [Re: JoshRoomar]
#18780967 - 08/31/13 11:17 AM (10 years, 4 months ago) |
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From JoshRoomar: I ordered a P. Atlantis mushroom spore vial from a reputable vendor. I inoculated two rye jars and four light malt extract (LME) dishes. It has been one month and I have seen no growth or contamination. I threw it all out. Why didn't I see growth in my jars?
JoshRoomar, I have not grown Psilocybe atlantis. From what I understand P. atlantis is closely related to P. Mexicana. There are many factors that could have resulted in failure. On rye substrates the moisture content and temperature can inhibit germination and growth. As for the LME I would have expected growth as long as there was no hydrogen peroxide (H2O2) added to the agar after the autoclave process to inhibit mold growth. As H2O2 would kill the spores. If the temperatures were in a range of 76-82F I would have expected to see growth within 3 weeks at the most. One issue that may delay or inhibit spore germination are severely dehydrated spores. It takes special care to attempt to rehydrate or germinate such spores even if they are transferred to aqueous solution. A microscope is the best tool to troubleshoot such issues. Spores sticking to the vial is usually not an issue since there are so many spores and only a few of them need to germinate. The age of the spores is probably not the issue although I have not worked with P. atlantis spores. Spores of P. cubensis can successfully be germinated after 20 years with ease as long as the spores have not desiccated.
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    I'm awesome sauce with a dash of sunshine.
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spore baby



Registered: 07/30/13
Posts: 4,918
Last seen: 8 years, 7 months
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Edited by spore baby (12/20/14 05:44 AM)
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