Home | Community | Message Board

Original Seeds Store
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Capsules   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineJoshRoomar
Lurker


Registered: 01/18/13
Posts: 24
Last seen: 10 years, 3 months
Is my syringe empty??
    #18780881 - 08/31/13 10:48 AM (10 years, 4 months ago)

It's been a while since my last post. I've been experimenting with a few teks and you guys really helped me a lot just from reading your day to day experiences, so my sincere gratitude to all of you smart shroomheads!
Now to my question. I've bought 10ml of sclerotia producer, Atlantis strain to be precise, from a trusted vendor. Now I say trusted, because it's one of the biggest out there and as far as my experience goes, I would assume I can "trust" this vendor. Now from the first look the vial looked empty, but I didn't pay much attention to it, as I know the spores are really small. So I knocked two rye jars and four LME agar dishes and hoped for the best. More than one month and nothing. Not even sign of contamination. So I tossed it away with the result, that there is just no spores in the solution. It never happened to me before and as I don't have microscope, there is no other way to determine if it's "empty" or not. Or is it?? Did this happen to you before?? And I didn't add coffee to it, might that be the problem (I would say no, but never did sclerotia before)?? Again thx for replies


Extras: Filter Print Post Top
OfflineTrippy_Penguin
 User Gallery


Registered: 03/18/12
Posts: 624
Last seen: 3 years, 5 months
Re: Is my syringe empty?? [Re: JoshRoomar]
    #18780899 - 08/31/13 10:55 AM (10 years, 4 months ago)

Did you shake it well prior to using it?

If you are going through a good vendor, you should email them and let them know the syringe was no good. Lots of reputable vendors will send you a new one if the original was bad.


Extras: Filter Print Post Top
InvisibleThe Psyentist
Figment of my Imagination
Male

Registered: 07/30/13
Posts: 82
Loc: Central Florida Flag
Re: Is my syringe empty?? [Re: JoshRoomar]
    #18780907 - 08/31/13 10:57 AM (10 years, 4 months ago)

I'm not saying it was bunk. But, I only trust sponsors of this website. You don't have to see spores for them to be in there. So without a microscope or results, it's hard to say.


--------------------


Extras: Filter Print Post Top
InvisibleFruitOfLife
Professional Package Handler


Registered: 05/21/12
Posts: 4,832
Re: Is my syringe empty?? [Re: Trippy_Penguin]
    #18780914 - 08/31/13 10:59 AM (10 years, 4 months ago)

Quote:

petersodm said:
Did you shake it well prior to using it?

If you are going through a good vendor, you should email them and let them know the syringe was no good. Lots of reputable vendors will send you a new one if the original was bad.



word it as "spores not viable" Dont say things like mycelium wont grow or anything related to growing. You dont want to get a vendor in trouble


--------------------


Extras: Filter Print Post Top
InvisibleThe Psyentist
Figment of my Imagination
Male

Registered: 07/30/13
Posts: 82
Loc: Central Florida Flag
Re: Is my syringe empty?? [Re: FruitOfLife]
    #18780947 - 08/31/13 11:10 AM (10 years, 4 months ago)

:whathesaid: also


--------------------


Extras: Filter Print Post Top
OfflineTrippy_Penguin
 User Gallery


Registered: 03/18/12
Posts: 624
Last seen: 3 years, 5 months
Re: Is my syringe empty?? [Re: FruitOfLife]
    #18780950 - 08/31/13 11:11 AM (10 years, 4 months ago)

Quote:

FruitOfLife said:

word it as "spores not viable" Dont say things like mycelium wont grow or anything related to growing.




Good call on that.


Extras: Filter Print Post Top
Offlinemastercultivator
Master Cultivator
 User Gallery


Registered: 08/27/13
Posts: 653
Last seen: 7 months, 2 days
Re: Is my syringe empty?? [Re: JoshRoomar]
    #18780967 - 08/31/13 11:17 AM (10 years, 4 months ago)

From JoshRoomar:
I ordered a P. Atlantis mushroom spore vial from a reputable vendor.  I inoculated two rye jars and four light malt extract (LME) dishes.  It has been one month and I have seen no growth or contamination.  I threw it all out.  Why didn't I see growth in my jars?

JoshRoomar,
I have not grown Psilocybe atlantis.  From what I understand P. atlantis is closely related to P. Mexicana.  There are many factors that could have resulted in failure. 
On rye substrates the moisture content and temperature can inhibit germination and growth.  As for the LME I would have expected growth as long as there was no hydrogen peroxide (H2O2) added to the agar after the autoclave process to inhibit mold growth.  As H2O2 would kill the spores.  If the temperatures were in a range of 76-82F I would have expected to see growth within 3 weeks at the most.  One issue that may delay or inhibit spore germination are severely dehydrated spores.  It takes special care to attempt to rehydrate or germinate such spores even if they are transferred to aqueous solution.  A microscope is the best tool to troubleshoot such issues.  Spores sticking to the vial is usually not an issue since there are so many spores and only a few of them need to germinate.  The age of the spores is probably not the issue although I have not worked with P. atlantis spores.  Spores of P. cubensis can successfully be germinated after 20 years with ease as long as the spores have not desiccated.


--------------------
:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:
I'm awesome sauce with a dash of sunshine.



Extras: Filter Print Post Top
Offlinespore baby
Male


Registered: 07/30/13
Posts: 4,918
Last seen: 8 years, 7 months
Re: Is my syringe empty?? [Re: mastercultivator]
    #18781762 - 08/31/13 03:44 PM (10 years, 4 months ago)

.


Edited by spore baby (12/20/14 05:44 AM)


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Capsules   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Re: spore sryinges empty? pl151 1,561 7 04/25/01 04:13 PM
by shizifty
* Greetings all! (n00b ps. atlantis questions) Mushroom Forest 590 8 09/18/09 12:35 PM
by Workman
* ANyone got an extra empty syringe? Slimz 1,025 8 03/09/08 04:18 PM
by P.Menace
* Newbie about to dive in head first - amount of spore syring CCs into 4 lb grow bag?
( 1 2 all )
ChrisHippyLoser 14,981 25 06/02/16 05:47 PM
by OldSawbonesHisself
* spore syring is in trouble? mulan 14,123 12 08/29/09 09:15 PM
by HaRo On KoRn
* PF Redspore syring help duderonomy 1,417 11 07/02/09 04:31 PM
by duderonomy
* quick spore syringe question (resolved) hoodbran 3,362 5 07/20/08 05:09 AM
by PowerOfTheCoir
* one syring per print with this tek? ismokeweed 2,860 13 02/05/08 11:06 PM
by Cyber

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
602 topic views. 38 members, 226 guests and 26 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.008 seconds on 14 queries.