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Offlineappl3juic3
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Registered: 08/15/13
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how to determine if contaminated
    #18775085 - 08/29/13 08:53 PM (10 years, 5 months ago)

I used a liquid culture using the honey tek and picked a specimen of choice and dropped a few of its gills in the honey culture jar and after 24 hrs i definitely see white hairs what i am confident is mycelium but there are little black specks in the jar as well and i know some of it is the gills but i want to know how i can know if theres contamination from w/e may have been on the gills


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Offlineappl3juic3
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Re: how to determine if contaminated [Re: appl3juic3]
    #18777629 - 08/30/13 02:24 PM (10 years, 5 months ago)

still smells fine and mycelium is growing i added some peroxide, is there a reason no one is commenting, like have i missed a rule, is there no good answers yet or is there general disgust for my semi sterile ways (all was sterilized except for the gills added to culture.


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OfflineJimmyhunter1000
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Re: how to determine if contaminated [Re: appl3juic3]
    #18778149 - 08/30/13 04:15 PM (10 years, 5 months ago)

Well you didn't add any pictures, and you're asking about if an LC is good or not. LCs cannot be IDed as healthy without first testing it on a jar/agar.

I'm also not sure why you added the gills to the LC instead of spores. Frankly I don't even recommend LC to begin with. I'd suggest doing some more reading of how to properly create one if you do plan on using it.


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Offlineappl3juic3
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Re: how to determine if contaminated [Re: Jimmyhunter1000]
    #18778250 - 08/30/13 04:38 PM (10 years, 5 months ago)

Well I'm not asking if my jars contaminated I'm asking if there are signs that I can watch for I didn't use just use spores because I have no access to a needle and am still waiting on a spore syringe I ordered but I have some fruit and knew there was spores in the gills. I also have read alot on lc's and wanted to try with what I could attain. I know it's not protocol or recommended but I've already done it I see mycelium I didn't add a pic cuz I doubt it'd help, I am just wondering if there are obv signs of contam in a lc and from ur response Im guessing not w/o introducing to substrate, is that right?


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Offlinevolk
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Re: how to determine if contaminated [Re: appl3juic3]
    #18778304 - 08/30/13 04:50 PM (10 years, 5 months ago)

from what i've read. yes, you have to test it to know.


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OfflineMAIA
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Re: how to determine if contaminated [Re: volk]
    #18778446 - 08/30/13 05:34 PM (10 years, 5 months ago)

You're missing some pieces of info:

1 - How did you collect the specimen ?
2 - How did you transfer the specimen ?
3 - How did you sterilized the medium ?

Otherwise, and without pics, it's hard to call.

Cheers,
maia


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Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
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Offlineappl3juic3
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Re: how to determine if contaminated [Re: MAIA]
    #18779030 - 08/30/13 07:52 PM (10 years, 5 months ago)

1. i picked it from a pasture
2. i used a flame sterilized pair of tweezers
3. i steam/boiled the honeywater for 30 mins as per the honeywater tek on this site

Not to be negative about it but i dont really see how that info helps, plz educate if im wrong but yeah i mean from what ive read on lc's and on contams one way would be if the jar has a foul odor but it smells like honeywater still idk i inoculated a pf jar and some carboard w it so suppose ill know soon enough. if anyone knows of signs to watch for it would be appreciated, and thx to all that have responded so far


Edited by appl3juic3 (08/30/13 07:55 PM)


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OfflineMAIA
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Re: how to determine if contaminated [Re: appl3juic3]
    #18779144 - 08/30/13 08:25 PM (10 years, 5 months ago)

Many people have succeeded with just boiling the hw for 30 mins. I've tried this tek a couple of times and it only worked the second time. Main difference, I sterilized the hw in the pc for 45 minutes.

Answers 1 and 2 give me some clues. First, the specimen is not sterile. You should "clean" it with a peroxide solution before transferring it to the medium. Otherwise, any contaminants attached to the specimen will have a chance to grow. Second, pair of sterilized tweezers, ok ! But did you use a glovebox to perform the transfer or was it open air ?

Try to remember this. Teks can give you the main direction to follow but there are some details you'll learn along the way that are not implicit to teks. Try to read as much as you can about sterile environments and sterile practices.


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Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


Edited by MAIA (08/30/13 08:26 PM)


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OfflineMAIA
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Re: how to determine if contaminated [Re: MAIA]
    #18779283 - 08/30/13 09:07 PM (10 years, 5 months ago)

One last thing. When working with outdoor, unclean specimens it's better to use part of the body for cloning, instead of gills and spores. Cut 1cm2 from the stem, clean the specimen with peroxide and use agar for cloning. There are plenty of teks for this process. HW should work as well using a piece of the stem but I have never tested it for this purpose.

LC's make sense if you're aiming for a fast, big yield because it doesn't last long but its growth is very fast, aggressive and you can spawn bigger amounts of substrate at once.

Agar cloning let's you play more comfortably with some aspects and IMHO it's better for the purpose of cloning and domesticating an outdoor specimen.


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Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
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Offlineappl3juic3
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Re: how to determine if contaminated [Re: MAIA]
    #18779738 - 08/30/13 11:27 PM (10 years, 5 months ago)

thanks alot thats the kind of response i was hoping for quite informative i have read many many versions of teks on this site of lc's spawning and pf mostly and have tried to find that kind of info from the search engine to only semi successfully its too bad all i have is a cap so a couple questions if i may what would be your recomendation if all you had was a small dryed cap? (and no syringe) and does the stem have to be fresh to clone from the stem? oh yeah yes i used a cardboard box glove box with gloves and cling wrap over top


Edited by appl3juic3 (08/30/13 11:38 PM)


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Offlineappl3juic3
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Re: how to determine if contaminated [Re: appl3juic3]
    #18779779 - 08/30/13 11:40 PM (10 years, 5 months ago)

and i was under the impression that spores were sensitive to peroxide that it was the mycelium that could handle peroxide was that incorrect?


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OfflineMAIA
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Re: how to determine if contaminated [Re: appl3juic3]
    #18795161 - 09/03/13 04:28 PM (10 years, 5 months ago)

Quote:

appl3juic3 said:
and i was under the impression that spores were sensitive to peroxide that it was the mycelium that could handle peroxide was that incorrect?




Yes spores are sensitive to peroxide. When using the HW tek, I would let the spores germinate first and then use peroxide if needed. But alas, you shouldn't need peroxide if working in a sterile environment.

Btw, that's another reason to not to use wild spores with HW.


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Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
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