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OfflinekmIL
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Potentially contammed monotub - its alive! Pin Pics!
    #18775857 - 08/30/13 01:45 AM (10 years, 4 months ago)

7-10 days ago they were about 90% and doing just fine.  Now they look like this?!

Color looks bright yellow, like it could be metabolites.  What do you guys think?



Quote:

kmIL said:
could this have to do with the fact that ive been using the same isolate for 3-4 grows now? i always make an extra jar to make a glc to knock up my next set. i recalled hearing a strain can only be cloned so many times... does that apply here even though im not really "cloning" when i propogate the isolate through glc?




Could this be a genetics issue?  This was from an isolate culture I've used for 5-6 cycles now.  Every time I would grow an extra jar to make a glc for the next cycle.  Would that cause genetic complication after multiple "generations"?


Edited by kmIL (09/20/13 12:49 AM)


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InvisibleSparchitect
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Re: What happened to my jars?! [Re: kmIL]
    #18775873 - 08/30/13 01:57 AM (10 years, 4 months ago)

Looks like contam to me. Wait for a more experienced member's input before you toss it though.

EDIT: Does it smell funky?


Edited by Sparchitect (08/30/13 02:01 AM)


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Invisiblewildernessjunkie
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Re: What happened to my jars?! [Re: Sparchitect]
    #18775882 - 08/30/13 02:04 AM (10 years, 4 months ago)

Looks like they need to be spawned ASAP.

Pic 3 looks like its forming blobs to me.


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Invisiblemycohumanity
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Re: What happened to my jars?! [Re: wildernessjunkie]
    #18776320 - 08/30/13 07:27 AM (10 years, 4 months ago)

Quote:

wildernessjunkie said:
Looks like they need to be spawned ASAP.

Pic 3 looks like its forming blobs to me.




^ what he said


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Invisibletbagtag
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Re: What happened to my jars?! [Re: mycohumanity] * 1
    #18776334 - 08/30/13 07:38 AM (10 years, 4 months ago)

They could turn out fine but my money is on bacterial contamination due to how prominent the discoloration is. They really need to put up the gambling board. ...

The blobs are probably in response to the contamination.  He said 7 to 10 day's ago they were at 90% which would make me assume they have only been colonized for 3 to 4 day's ago. Most likely that shit is going turn nasty.


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OfflineMidnight Cyclone
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Re: What happened to my jars?! [Re: tbagtag]
    #18776355 - 08/30/13 07:48 AM (10 years, 4 months ago)

Quote:

tbagtag said:
They could turn out fine but my money is on bacterial contamination due to how prominent the discoloration is.




My thoughts exactly.


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OfflinekmIL
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Re: What happened to my jars?! [Re: wildernessjunkie]
    #18779770 - 08/30/13 11:37 PM (10 years, 4 months ago)

Quote:

wildernessjunkie said:
Looks like they need to be spawned ASAP.

Pic 3 looks like its forming blobs to me.




Do the blobs indicate over colonization or bacterial contamination? :confused:


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Offlinetropicalfrenzy
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Re: What happened to my jars?! [Re: kmIL]
    #18779790 - 08/30/13 11:44 PM (10 years, 4 months ago)

Quote:

kmIL said:
Quote:

wildernessjunkie said:
Looks like they need to be spawned ASAP.

Pic 3 looks like its forming blobs to me.




Do the blobs indicate over colonization or bacterial contamination? :confused:



this is dependent on strain and temperature... Blobs are generally not contamination but mutation. I guess contam given the time frame. One sniff of the jar will give you the truth of it though.

edit: at a glance it looks like blobs though... I agree with wildernessjunkie.

edit edit: i know I contradicted myself there... Just give it a sniff and you will know.


--------------------


Edited by tropicalfrenzy (08/30/13 11:49 PM)


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Invisibletbagtag
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Re: What happened to my jars?! [Re: tropicalfrenzy]
    #18779844 - 08/31/13 12:04 AM (10 years, 4 months ago)

Op, I get the vibe you're looking for optimistic answers.  Spawn it and document it for the community.  I don't mean to bet against you, it's cuz I care that I'm about to say the following.

I got 2 thai lipa yai prints that say this shit turns more green than the hulk by the time the first flush finishes. Anyone who can view spores LEGALLY want to take the bet?


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Invisibletbagtag
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Re: What happened to my jars?! [Re: tbagtag]
    #18779860 - 08/31/13 12:11 AM (10 years, 4 months ago)

Op to sweeten the deal I will give you a vendor made syringe from my collection - if you meet 2 conditions

1 You live in the continental US in a state that it is legal to view

2 You document the grow up to the completion of the first flush or it fails which ever happens first.


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Invisibletbagtag
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Re: What happened to my jars?! [Re: tbagtag]
    #18782609 - 08/31/13 07:29 PM (10 years, 4 months ago)

No takers?  OP you interested?  You got nothing to lose if you're going to get a syringe out of the deal anyway.


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OfflineSockadin
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Re: What happened to my jars?! [Re: tbagtag]
    #18783313 - 08/31/13 10:16 PM (10 years, 4 months ago)

I have experienced this before when I played with Corn, some say contam, some say metabolites. All I know is that if it don't stink run with it for a while.


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Offlinerockstaradam
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Re: What happened to my jars?! [Re: Sockadin]
    #18783397 - 08/31/13 10:37 PM (10 years, 4 months ago)

Spawn those now!!


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OfflinekmIL
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Re: What happened to my jars?! [Re: rockstaradam]
    #18796049 - 09/03/13 07:46 PM (10 years, 4 months ago)

Ha, I am a betting man but I do not live in a state where viewing spores is legal :frown:

These jars don't small contaminated.  They have the nice fungus smell that I'm used to.  I'll give it a go and spawn these to a coir substrate.

Very odd metabolites / blobbing though.  I have had jars over colonize before and they didn't act like this. 

Very strange.  I'll keep the thread posted.  Will be spawning tomorrow / maybe later tonight if my substrate is cool enough :thumbup:


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OfflinekmIL
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Re: What happened to my jars?! [Re: kmIL]
    #18799568 - 09/04/13 02:52 PM (10 years, 4 months ago)

could this have to do with the fact that ive been using the same isolate for 3-4 grows now? i always make an extra jar to make a glc to knock up my next set. i recalled hearing a strain can only be cloned so many times... does that apply here even though im not really "cloning" when i propogate the isolate through glc?


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OfflinebEelzeBosS
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Re: What happened to my jars?! [Re: kmIL]
    #18799577 - 09/04/13 02:55 PM (10 years, 4 months ago)

Give it a good shake then smell the FAE hole. If it smells like mushrooms and the jar recovers in a couple days its likely fine.


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OfflinekmIL
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Re: What happened to my jars?! [Re: kmIL]
    #18800700 - 09/04/13 07:42 PM (10 years, 4 months ago)

I already spawned it to coir.  Smelled like mushrooms to me, maybe a little sourness, but not sure if that was head playing tricks with me?

the discoloration appeared to be where the myc had blobbed and come up against the jar.

I'll post an update in about a week and we'll see if it's gonna work.


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OfflinekmIL
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Re: What happened to my jars?! [Re: kmIL]
    #18809158 - 09/06/13 08:10 PM (10 years, 4 months ago)

updated OP!


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OfflinekmIL
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Re: What happened to my jars?! [Re: kmIL]
    #18833941 - 09/12/13 07:38 PM (10 years, 4 months ago)

So it's been about 8 days and the monotub looks good.  I'm guessing these jars were not contaminated but rather over colonized.

If there is any interest in this thread I'll write up my notes and give a detailed account of the jars and spawning process. 

If not, I'll just post pics to indicate results!  Cheers!



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Offlinetropicalfrenzy
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Re: What happened to my jars?! [Re: kmIL]
    #18852249 - 09/17/13 02:24 AM (10 years, 4 months ago)

pinning yet?


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OfflinekmIL
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Re: What happened to my jars?! [Re: tropicalfrenzy]
    #18865948 - 09/20/13 12:47 AM (10 years, 4 months ago)

Pins!

Looks like a perfectly healthy monotub.  Guess those jars weren't contammed after all :mushroom2:



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OfflineMush4Brains
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Re: What happened to my jars?! [Re: kmIL]
    #18865989 - 09/20/13 01:09 AM (10 years, 4 months ago)

Hopefully they mature. :trippinbawelz:


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OfflinekmIL
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Re: What happened to my jars?! [Re: Mush4Brains]
    #18868223 - 09/20/13 03:24 PM (10 years, 4 months ago)

Im giving this a high probability for success!

Will post pics Sunday.

Interestingly this tub will have to finish up in a dark storage room. Im interested to see how yhe absence of light affects the flush.

This one is for science!!


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OfflineMidnight Cyclone
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Re: What happened to my jars?! [Re: kmIL]
    #18874854 - 09/22/13 06:45 AM (10 years, 4 months ago)

You'll know for sure whether it's contamed or not in a day or two.

Either they won't fully develop and you'll have a bunch of aborts, or you'll have full grown healthy mushrooms.

Keep us posted!


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OfflinekmIL
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Re: What happened to my jars?! [Re: Midnight Cyclone]
    #18881927 - 09/23/13 07:32 PM (10 years, 4 months ago)

Quote:

Mush4Brains said:
Hopefully they mature. :trippinbawelz:




Quote:

Midnight Cyclone said:
You'll know for sure whether it's contamed or not in a day or two.

Either they won't fully develop and you'll have a bunch of aborts, or you'll have full grown healthy mushrooms.

Keep us posted!




+2 pts for you guys, you were spot on.  I am unfortunately looking at a monotub FULL of aborts.

Once I saw how fast the spawn colonized the coir and the healthy pin set I thought it was in the clear, but it looks like the contam stopped my fruits from maturing. 

Learned a lot from this experiment though.  Contams aren't necessarily going to throw a wrench in your grow right away, but they will certainly stop you eventually!

Interesting too that symptoms of the contamination didn't manifest themselves until very late in the colonization process.  Like I said, these jars were ripping through my rye jars and I dind't even notice any signs of potential contamination until well after they were 100% colonized.

I'm thinking my grain LC somehow got contaminated, which is a shame because I greenlighted it half way through this experiment and knocked up more jars with it :frown:

I'll let them go and see if they don't come out healthy, but if they turn out looking like the jars above, STRAIGHT IN THE TRASH.

Thanks for all the input, community.



Edit: On second glance, I can't figure out if this is a contam issue or due to the severe neglect for the tub over the last 4 days. 

I checked this yesterday and assumed it wast toast so I left it in storage where it had been over the weekend since I was out of town and had a guest staying in my house.

When I went to grab it today to take these pics for the community, I noticed it had grown some.  My polyfil was WAY too tight and it was in a dark, unventiliated room, about 10x18ft, certianly not ideal.

Is that what you guys would have expected for "aborts" or does this look more like severe lack of FAE / no light, which were, unfortunately, the fruiting conditions for this tub.

Feel free to chime in with what you think.


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OfflinekmIL
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Re: What happened to my jars?! [Re: kmIL]
    #18881942 - 09/23/13 07:35 PM (10 years, 4 months ago)

I gave the tub a good fan and have it sitting by a window now, will see if this thing picks up within the next few days, if not, I'm gonna toss it.

I also seriously loosened the polyfil to allow for more FAE.


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Re: What happened to my jars?! [Re: kmIL]
    #18882047 - 09/23/13 07:59 PM (10 years, 4 months ago)

Aborts are going to be really small and usually have a black cap to them. That looks more like a neglected woman in a bad relationship!


Skinny fruits happen from a lack of light!


Edited by myplague240 (09/23/13 08:02 PM)


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InvisibleFrankHorrigan
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Re: What happened to my jars?! [Re: myplague240]
    #18882055 - 09/23/13 08:01 PM (10 years, 4 months ago)

Why would you toss a tub that is not only producing mushrooms but has no visible green mold?

That's silly. Give them more fresh air. They look fine.


--------------------

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OfflineTheStormsEye
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Re: What happened to my jars?! [Re: FrankHorrigan]
    #18882065 - 09/23/13 08:02 PM (10 years, 4 months ago)

Quote:

FrankHorrigan said:
Why would you toss a tub that is not only producing mushrooms but has no visible green mold?

That's silly. Give them more fresh air. They look fine.




hell yea :whathesaid:


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OfflineMidnight Cyclone
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Re: What happened to my jars?! [Re: FrankHorrigan]
    #18883916 - 09/24/13 08:44 AM (10 years, 4 months ago)

Quote:

FrankHorrigan said:
Give them more fresh air. They look fine.




Tmethyl's "how it should and shouldn't look" would say the same thing. Long, skinny stipe and small caps = lack of FAE.

Keep that tub fruiting, it appears to be doing alright (minus the FAE issue) and only time will tell!

Good luck! :thumbup:


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OfflinekmIL
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Re: What happened to my jars?! [Re: Midnight Cyclone]
    #18884806 - 09/24/13 12:55 PM (10 years, 4 months ago)

I appreciate the encouragement but these pics are from t +5 days from the pinset pics. These guys might be toast from neglect. Ill update tonight abd well see if theyve grown any. Im tempted to pick these skinny boomers and hope for a nice 2nd flush. Thoughts?


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InvisibleFrankHorrigan
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Re: What happened to my jars?! [Re: kmIL]
    #18884820 - 09/24/13 12:57 PM (10 years, 4 months ago)

I wonder how your fruiting conditions are, myself.

Have you dialed in your monotub?

They are desperate for fresh air but they don't look aborted.


--------------------

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You should take a look. :hehehe:


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OfflinekmIL
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Re: What happened to my jars?! [Re: FrankHorrigan]
    #18885424 - 09/24/13 03:26 PM (10 years, 4 months ago)

Quote:

FrankHorrigan said:
I wonder how your fruiting conditions are, myself.

Have you dialed in your monotub?

They are desperate for fresh air but they don't look aborted.




I did click on the link your sig and found your post on dialing in a monotub. Very helpful and ill definitely is it for my next tub. I had to stash this box in a small unlight and unventilated room for 3 nights 4 days.  Should have losened the polyfil considerably before leaving it like that but i didnt :frown:. It was definitely getting very little fae those days which is why these fruits barely developed. I was worried i had too much fae and that my subs were drying out so the motto for this tub was tighter ppolyfil. More humidity at the expense of fae. Given i had to store this guy in less than optimal conditions, i should have switched the directive to assure fae at the expense of humidity.

Ill post an update later and well see if these guys have grown at all or if they have stalled out for good.  Looking forward to really dialing in my mt next go :smile:


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InvisibleFrankHorrigan
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Re: What happened to my jars?! [Re: kmIL]
    #18885744 - 09/24/13 04:40 PM (10 years, 4 months ago)

Are you running a fan in the room?

Monos do not work right without one.

That is most likely your issue.


--------------------

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You should take a look. :hehehe:


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OfflinekmIL
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Re: What happened to my jars?! [Re: FrankHorrigan]
    #18887331 - 09/24/13 11:29 PM (10 years, 4 months ago)

Looks like they are getting bigger :thumbup:



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OfflinekmIL
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Re: What happened to my jars?! [Re: kmIL]
    #18891855 - 09/25/13 11:05 PM (10 years, 4 months ago)

So I threw this monotub away.

I've had some good success with monotubs before and have plenty of fungus to show for it.  I'm in this for my masterpiece grow and it was clear this wasn't going to be it.  These guys just weren't recovering and it was evident these were going to be some DINKY boomers due to the neglect.

I just needed to confirm that my culture wasn't contaminated so that I could continue with the jars I have going and attempt my final grow. Hoping to really dial in my MT / fruiting conditions so that I can get some good forearmed-sized boomers.

Thanks for everybodys input who posted here, hopefully this will prove to be helpful to the community.


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OfflineTheStormsEye
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Re: What happened to my jars?! [Re: kmIL]
    #18891873 - 09/25/13 11:11 PM (10 years, 4 months ago)

You threw it away??
:confused:


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OfflineTheStormsEye
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Re: What happened to my jars?! [Re: kmIL]
    #18891887 - 09/25/13 11:17 PM (10 years, 4 months ago)

Quote:

kmIL said:
Pins!

Looks like a perfectly healthy monotub.





Quote:

kmIL said:
So I threw this monotub away.





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Re: What happened to my jars?! [Re: TheStormsEye]
    #18891949 - 09/25/13 11:37 PM (10 years, 4 months ago)

You threw away a perfectly healthy tub that needed more fresh air.

That's a damn shame, dude. :facepalm:


--------------------

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OfflineBlake_Shroom
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Re: What happened to my jars?! [Re: FrankHorrigan]
    #18893024 - 09/26/13 08:17 AM (10 years, 4 months ago)

Worst comes to worse, just put it outside and let it finish fruiting:facepalm3:

You potentially threw away 2+ oz. But at least you did get some, and I bet they'll be potent as fuck


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Re: What happened to my jars?! [Re: Blake_Shroom]
    #18893059 - 09/26/13 08:27 AM (10 years, 4 months ago)

Aw man, bummed you threw them out :C I was watching this and hoping they'd make a come back.


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OfflinekmIL
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Re: What happened to my jars?! [Re: KatuXiK]
    #18894906 - 09/26/13 04:43 PM (10 years, 4 months ago)

They may have come out "okay" but clearly damage had been done. Too many pins maturing and too skinny. I have plenty of nice fruits from other grows. Im more in it to perfect my process than for acquiring fruits. Sad, i know :frown:


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InvisibleKatuXiK
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Re: What happened to my jars?! [Re: kmIL]
    #18899570 - 09/27/13 04:59 PM (10 years, 4 months ago)

No reason to throw out skinny fruits though! Besides, I've seen some really fucked up first flushes on here that had amazing 2nd flushes. I guess I'm on my first grow and have no supply at all, so I'm probably a little less caring about how my shrooms look, and more about whether or not they actually survive. :P


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