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OfflinekmIL
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Registered: 10/06/05
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Potentially contammed monotub - its alive! Pin Pics!
    #18775857 - 08/30/13 01:45 AM (10 years, 4 months ago)

7-10 days ago they were about 90% and doing just fine.  Now they look like this?!

Color looks bright yellow, like it could be metabolites.  What do you guys think?



Quote:

kmIL said:
could this have to do with the fact that ive been using the same isolate for 3-4 grows now? i always make an extra jar to make a glc to knock up my next set. i recalled hearing a strain can only be cloned so many times... does that apply here even though im not really "cloning" when i propogate the isolate through glc?




Could this be a genetics issue?  This was from an isolate culture I've used for 5-6 cycles now.  Every time I would grow an extra jar to make a glc for the next cycle.  Would that cause genetic complication after multiple "generations"?


Edited by kmIL (09/20/13 12:49 AM)


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InvisibleSparchitect
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Registered: 01/07/13
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Re: What happened to my jars?! [Re: kmIL]
    #18775873 - 08/30/13 01:57 AM (10 years, 4 months ago)

Looks like contam to me. Wait for a more experienced member's input before you toss it though.

EDIT: Does it smell funky?


Edited by Sparchitect (08/30/13 02:01 AM)


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Invisiblewildernessjunkie
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Registered: 06/13/10
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Re: What happened to my jars?! [Re: Sparchitect]
    #18775882 - 08/30/13 02:04 AM (10 years, 4 months ago)

Looks like they need to be spawned ASAP.

Pic 3 looks like its forming blobs to me.


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Invisiblemycohumanity
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Registered: 06/21/13
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Re: What happened to my jars?! [Re: wildernessjunkie]
    #18776320 - 08/30/13 07:27 AM (10 years, 4 months ago)

Quote:

wildernessjunkie said:
Looks like they need to be spawned ASAP.

Pic 3 looks like its forming blobs to me.




^ what he said


--------------------
Stuff & Things



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Invisibletbagtag
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Registered: 01/16/13
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Re: What happened to my jars?! [Re: mycohumanity] * 1
    #18776334 - 08/30/13 07:38 AM (10 years, 4 months ago)

They could turn out fine but my money is on bacterial contamination due to how prominent the discoloration is. They really need to put up the gambling board. ...

The blobs are probably in response to the contamination.  He said 7 to 10 day's ago they were at 90% which would make me assume they have only been colonized for 3 to 4 day's ago. Most likely that shit is going turn nasty.


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OfflineMidnight Cyclone
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Re: What happened to my jars?! [Re: tbagtag]
    #18776355 - 08/30/13 07:48 AM (10 years, 4 months ago)

Quote:

tbagtag said:
They could turn out fine but my money is on bacterial contamination due to how prominent the discoloration is.




My thoughts exactly.


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OfflinekmIL
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Re: What happened to my jars?! [Re: wildernessjunkie]
    #18779770 - 08/30/13 11:37 PM (10 years, 4 months ago)

Quote:

wildernessjunkie said:
Looks like they need to be spawned ASAP.

Pic 3 looks like its forming blobs to me.




Do the blobs indicate over colonization or bacterial contamination? :confused:


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Offlinetropicalfrenzy
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Re: What happened to my jars?! [Re: kmIL]
    #18779790 - 08/30/13 11:44 PM (10 years, 4 months ago)

Quote:

kmIL said:
Quote:

wildernessjunkie said:
Looks like they need to be spawned ASAP.

Pic 3 looks like its forming blobs to me.




Do the blobs indicate over colonization or bacterial contamination? :confused:



this is dependent on strain and temperature... Blobs are generally not contamination but mutation. I guess contam given the time frame. One sniff of the jar will give you the truth of it though.

edit: at a glance it looks like blobs though... I agree with wildernessjunkie.

edit edit: i know I contradicted myself there... Just give it a sniff and you will know.


--------------------


Edited by tropicalfrenzy (08/30/13 11:49 PM)


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Invisibletbagtag
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Re: What happened to my jars?! [Re: tropicalfrenzy]
    #18779844 - 08/31/13 12:04 AM (10 years, 4 months ago)

Op, I get the vibe you're looking for optimistic answers.  Spawn it and document it for the community.  I don't mean to bet against you, it's cuz I care that I'm about to say the following.

I got 2 thai lipa yai prints that say this shit turns more green than the hulk by the time the first flush finishes. Anyone who can view spores LEGALLY want to take the bet?


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Invisibletbagtag
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Re: What happened to my jars?! [Re: tbagtag]
    #18779860 - 08/31/13 12:11 AM (10 years, 4 months ago)

Op to sweeten the deal I will give you a vendor made syringe from my collection - if you meet 2 conditions

1 You live in the continental US in a state that it is legal to view

2 You document the grow up to the completion of the first flush or it fails which ever happens first.


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Invisibletbagtag
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Re: What happened to my jars?! [Re: tbagtag]
    #18782609 - 08/31/13 07:29 PM (10 years, 4 months ago)

No takers?  OP you interested?  You got nothing to lose if you're going to get a syringe out of the deal anyway.


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OfflineSockadin
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Registered: 01/03/10
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Re: What happened to my jars?! [Re: tbagtag]
    #18783313 - 08/31/13 10:16 PM (10 years, 4 months ago)

I have experienced this before when I played with Corn, some say contam, some say metabolites. All I know is that if it don't stink run with it for a while.


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Offlinerockstaradam
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Re: What happened to my jars?! [Re: Sockadin]
    #18783397 - 08/31/13 10:37 PM (10 years, 4 months ago)

Spawn those now!!


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OfflinekmIL
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Re: What happened to my jars?! [Re: rockstaradam]
    #18796049 - 09/03/13 07:46 PM (10 years, 4 months ago)

Ha, I am a betting man but I do not live in a state where viewing spores is legal :frown:

These jars don't small contaminated.  They have the nice fungus smell that I'm used to.  I'll give it a go and spawn these to a coir substrate.

Very odd metabolites / blobbing though.  I have had jars over colonize before and they didn't act like this. 

Very strange.  I'll keep the thread posted.  Will be spawning tomorrow / maybe later tonight if my substrate is cool enough :thumbup:


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OfflinekmIL
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Re: What happened to my jars?! [Re: kmIL]
    #18799568 - 09/04/13 02:52 PM (10 years, 4 months ago)

could this have to do with the fact that ive been using the same isolate for 3-4 grows now? i always make an extra jar to make a glc to knock up my next set. i recalled hearing a strain can only be cloned so many times... does that apply here even though im not really "cloning" when i propogate the isolate through glc?


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OfflinebEelzeBosS
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Registered: 06/15/13
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Re: What happened to my jars?! [Re: kmIL]
    #18799577 - 09/04/13 02:55 PM (10 years, 4 months ago)

Give it a good shake then smell the FAE hole. If it smells like mushrooms and the jar recovers in a couple days its likely fine.


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OfflinekmIL
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Re: What happened to my jars?! [Re: kmIL]
    #18800700 - 09/04/13 07:42 PM (10 years, 4 months ago)

I already spawned it to coir.  Smelled like mushrooms to me, maybe a little sourness, but not sure if that was head playing tricks with me?

the discoloration appeared to be where the myc had blobbed and come up against the jar.

I'll post an update in about a week and we'll see if it's gonna work.


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OfflinekmIL
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Re: What happened to my jars?! [Re: kmIL]
    #18809158 - 09/06/13 08:10 PM (10 years, 4 months ago)

updated OP!


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OfflinekmIL
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Re: What happened to my jars?! [Re: kmIL]
    #18833941 - 09/12/13 07:38 PM (10 years, 4 months ago)

So it's been about 8 days and the monotub looks good.  I'm guessing these jars were not contaminated but rather over colonized.

If there is any interest in this thread I'll write up my notes and give a detailed account of the jars and spawning process. 

If not, I'll just post pics to indicate results!  Cheers!



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Offlinetropicalfrenzy
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Re: What happened to my jars?! [Re: kmIL]
    #18852249 - 09/17/13 02:24 AM (10 years, 4 months ago)

pinning yet?


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