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Goomba_trooper
Stranger

Registered: 08/29/13
Posts: 1
Last seen: 10 years, 5 months
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mushroom cultivation
#18775309 - 08/29/13 09:45 PM (10 years, 5 months ago) |
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I'm a very inexperienced grower but since this is for advanced mycologists i figured an answer could be found here. Would it be possible to cross breed mushrooms similar to the cross strains of marijuana?
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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No....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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b plus

Registered: 10/13/12
Posts: 928
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Re: mushroom cultivation [Re: PussyFart]
#18775835 - 08/30/13 01:31 AM (10 years, 5 months ago) |
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Quote:
Notahacker420 said: No....

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MacMerdin
Hunter



Registered: 04/26/12
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Loc: The Island of Misfit Toys
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Re: mushroom cultivation [Re: PussyFart]
#18776148 - 08/30/13 05:19 AM (10 years, 5 months ago) |
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EDIT:
I read the OP incorrectly.
Edited by MacMerdin (08/30/13 05:21 AM)
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: mushroom cultivation [Re: MacMerdin]
#18779442 - 08/30/13 09:55 PM (10 years, 5 months ago) |
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All sexually reproducing organisms work in the same way genetically. Chromosomes exist in pairs (at least at some point in the life cycle) and each child receives exactly 50% of it's DNA from each parent. (notwithstanding chromosomal size differences)
There is still lots of confusion, misunderstandings, and downright disinformation on mushrooms relating to this issue for some reason.
The details of the lifecycle can vary, but the genetic results are the same in fungi, plants, and animals.
-FF
-------------------- It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed "If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP) I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
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drake89
Mushroom Magnate



Registered: 06/26/11
Posts: 4,168
Loc: TN
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Re: mushroom cultivation [Re: fastfred]
#18780891 - 08/31/13 10:52 AM (10 years, 5 months ago) |
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have any of the naysayers ever visited an actives spore website? where they sell STRAINS?
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mastercultivator
Master Cultivator



Registered: 08/27/13
Posts: 653
Last seen: 7 months, 27 days
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Re: mushroom cultivation [Re: fastfred]
#18781082 - 08/31/13 11:53 AM (10 years, 5 months ago) |
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Quote:
fastfred said: All sexually reproducing organisms work in the same way genetically. Chromosomes exist in pairs (at least at some point in the life cycle) and each child receives exactly 50% of it's DNA from each parent. (notwithstanding chromosomal size differences)
There is still lots of confusion, misunderstandings, and downright disinformation on mushrooms relating to this issue for some reason.
The details of the lifecycle can vary, but the genetic results are the same in fungi, plants, and animals.
-FF
I'm with FF. Note though that in a MS inoculation it is like a city of people working together. Soo spore race is more ap-pro-pro
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    I'm awesome sauce with a dash of sunshine.
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mastercultivator
Master Cultivator



Registered: 08/27/13
Posts: 653
Last seen: 7 months, 27 days
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Quote:
Goomba_trooper said: I'm a very inexperienced grower but since this is for advanced mycologists i figured an answer could be found here. Would it be possible to cross breed mushrooms similar to the cross strains of marijuana?
Goomba_trooper,
Yes you can fuse mycellium or mate individual spores under a microscope. It can be done. Mating individual spores under a microscope is tedious though. I've never bothered. Spores can be mixed from two strains and grown out. Some mate with their own type, some cross mate, and some mycelium fuses. You end up with mushrooms that look different than the two original strains. You can take spores and grow them out a few times and you have a new strain that isn't like either of the parent strains. It is best to dilute them heavily and test the dilution and do a test germination under a microscope a drop of spores and a drop of sugar water as well as 6 hours to watch the spores germinate is all you need. You are seeing if the spores of both strains germinate at about the same time and mate. Then throw the spore solution you tested on agar and keep it at the same temp you germinated the spores on your slide. Watch it grow. Take a wedge or some rizomorphic growth at the edge of a colony and grow it out and see what you get.
At this point it is smarter and easier to start with a good strand like PE or another strain you like and find a meaty and potent clone and store lots of plates. Then make sure the clone is good and keep a few plates growing slowly in a fridge and grow out a lot of it.
My 2cents. Contact me if you need guidance. I'm not sure what you are trying to do.
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    I'm awesome sauce with a dash of sunshine.
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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The clamp connection is an interesting feature of mated myc. They control the migration of the nuclei during cell division.
Here's a pic of my own...

Here's a little explanation...

-FF
-------------------- It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed "If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP) I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
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TwinEclipse
Psychedelic Alchemist


Registered: 07/06/13
Posts: 1,499
Loc: NGC1097
Last seen: 8 years, 6 months
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Re: mushroom cultivation [Re: fastfred]
#18785006 - 09/01/13 11:54 AM (10 years, 5 months ago) |
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How are so many psilocybin species produced in the first place? What makes new breeds of mushrooms? I've never thought of that. Gonna goooogle it
-------------------- My purpose: to love, to share, and to experience....all while conforming to my psychedelic experiences.
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,312
Last seen: 4 days, 2 hours
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This thread was moved from Advanced Mycology.
Reason: Fits better here
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PhosCap
Gratuitous Heavenly Grace


Registered: 06/09/10
Posts: 975
Loc: Tartary
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So if the theory is correct, can you cross a Psilocybe Cubensis with another Psilocybe.. Cyan or Asurescens for instance?
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rockstaradam
Stranger

Registered: 08/13/12
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Re: mushroom cultivation (moved) [Re: PhosCap]
#18787427 - 09/01/13 10:14 PM (10 years, 5 months ago) |
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Can I have a baby with a tree?
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,312
Last seen: 4 days, 2 hours
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Re: mushroom cultivation (moved) [Re: PhosCap]
#18787551 - 09/01/13 10:46 PM (10 years, 5 months ago) |
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Quote:
phosi said: So if the theory is correct, can you cross a Psilocybe Cubensis with another Psilocybe.. Cyan or Asurescens for instance?
It would be better to cross species that were more closely related. You can use genbank/BLAST to find out which species are closely related.
Traditionally, Psilocybe phylogenetics were inferred mostly from spore shape. DNA sequencing has proven that this is not a good way to try to determine which ones are closely related.
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PhosCap
Gratuitous Heavenly Grace


Registered: 06/09/10
Posts: 975
Loc: Tartary
Last seen: 9 months, 8 days
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Re: mushroom cultivation (moved) [Re: rockstaradam]
#18787673 - 09/01/13 11:20 PM (10 years, 5 months ago) |
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Quote:
rockstaradam said: Can I have a baby with a tree?
I already knew the situation before questioning No its more like can you cross and horse and a dog. The answer is probably yes by some advanced DNA biologist. Its scary what they are doing these days.
Edited by PhosCap (09/01/13 11:21 PM)
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PhosCap
Gratuitous Heavenly Grace


Registered: 06/09/10
Posts: 975
Loc: Tartary
Last seen: 9 months, 8 days
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Quote:
Alan Rockefeller said:
Quote:
phosi said: So if the theory is correct, can you cross a Psilocybe Cubensis with another Psilocybe.. Cyan or Asurescens for instance?
It would be better to cross species that were more closely related. You can use genbank/BLAST to find out which species are closely related.
Traditionally, Psilocybe phylogenetics were inferred mostly from spore shape. DNA sequencing has proven that this is not a good way to try to determine which ones are closely related.
Interesting.. thanks.
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