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mycomattie



Registered: 11/15/12
Posts: 1,323
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PE6 - Print -> Agar -> Transfer -> Grain -> Monotub
#18772172 - 08/29/13 08:55 AM (10 years, 5 months ago) |
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It's been a while Shroomery - I've been pretty inactive June, July and August; had some serious family issues go down. I had a very promising Gymnopilus lute and Panaelous cyanescens (Hawaii) grows going on, but due to severe neglect and half assed pasteurization, they both succumbed to trich; which bummed me the fuck out even more. Now that things have settled down, I’m trying to get back into the swing of things!
This grow is a first of it’s kind for me – my first spore print -> agar -> transfer -> grain -> monotub. An awesome fellow Shroomery member, 36fucking5, sends me some very nice prints; this was a PE6 print.

I purchased MEA from Fungi Perfecti and plastic petri dishes/disposable scalpels from Amazon. I followed the directions on agar jar, let cool, and inoculated the plates in a SAB.
Honestly, my first attempts at agar are mediocre and awkward; the SAB really limits your dexterity. I transferred some of the most rhizomorphic sectors (for practice).

Once I felt I had some decent agar plates to work with (rhizo and contam free); I prepared quart jars of rye berries – pressure cooked for 90 minutes at 15psi and let cool overnight.
I’ve G2G’s before, but this would be my first time transferring agar wedges to grain. Again, it was awkward and clumsy working in the SAB, at least for me.
Shook the agar wedges and grain and let them sit, on a shelf, at room temp; and sit they did – probably 6 weeks past 100%.
I finally spawned them to bulk on August 15th. These definitely needed a soak per Frank Horrigans - Dunk Your Grain Tek. I spawned 5 quarts to ~ 7lbs of a manure based mix. I also used a black, heavy duty, trash bag liner.
This was my first time growing using a traditional monotub – set and forget style. Call it beginners luck (so far), but I definitely like them – no ultrasonic humidifier to refill, etc.
 
The bulk sub was ~ 100% colonized by the 22nd – only 7 days after spawning! On the 22nd I initiated fruiting.
This is today, August 29th – 7 days after tub was put into fruiting conditions.
 
Sorry for the lack of pics, but I didn't really bother to take any because I wasn't sure if I'd even bother with this grow.
8/30/13
Not seeing any resemblance to PE

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Edited by mycomattie (08/30/13 12:06 PM)
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JaredTheJeweler
it can only be me



Registered: 03/26/13
Posts: 54
Last seen: 7 months, 17 days
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Re: PE6 - Print -> Agar -> Transfer -> Grain -> Monotub [Re: mycomattie]
#18772226 - 08/29/13 09:28 AM (10 years, 5 months ago) |
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Lookin good! Im about to try my luck with agar today for the first time. Using a print just as you did too. Did you use anything special to transfer your spores to agar such as a inoculation loop?
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: PE6 - Print -> Agar -> Transfer -> Grain -> Monotub [Re: JaredTheJeweler]
#18772241 - 08/29/13 09:35 AM (10 years, 5 months ago) |
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Quote:
JaredTheJeweler said: Lookin good! Im about to try my luck with agar today for the first time. Using a print just as you did too. Did you use anything special to transfer your spores to agar such as a inoculation loop?
Thanks
No, I forgot to order one. I just used a heavier gauge sewing needle and flame sterilized; seemed to work OK - not that I have anything to compare it too.
Good luck with the agar!
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: PE6 - Print -> Agar -> Transfer -> Grain -> Monotub [Re: mycomattie]
#18772250 - 08/29/13 09:40 AM (10 years, 5 months ago) |
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I love agar. It may seem a bit awkward at first, but that part gets easier with practice. Then you get the benefit of knowing you have clean inoculate, and the ability to select faster growth can really pay off, even if you don't isolate. I always notice a difference in colonization speed and health, even with a culture that only has been transferred once or twice compared to randomly shooting some ms solution.
Im thinking of doing some PE6 soon myself, so I will be watching this one.
Good luck
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: PE6 - Print -> Agar -> Transfer -> Grain -> Monotub [Re: Pastywhyte]
#18772272 - 08/29/13 09:48 AM (10 years, 5 months ago) |
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Quote:
Pastywhyte said: I love agar. It may seem a bit awkward at first, but that part gets easier with practice. Then you get the benefit of knowing you have clean inoculate, and the ability to select faster growth can really pay off, even if you don't isolate. I always notice a difference in colonization speed and health, even with a culture that only has been transferred once or twice compared to randomly shooting some ms solution.
Im thinking of doing some PE6 soon myself, so I will be watching this one.
Good luck 
Thanks man! Yeah, agar definitely opens up a lot more doors, so it's worth it to get decent at it. I've gotten a little better : )
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: PE6 - Print -> Agar -> Transfer -> Grain -> Monotub [Re: mycomattie]
#18780540 - 08/31/13 08:22 AM (10 years, 4 months ago) |
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8/31/13
Almost ready to pick - I think I might not have the FAE dialed in right, they look a bit stretched and spindly (unless that's a common trait of PE6).
  
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TasteTheSound
Cankle Lover


Registered: 08/05/12
Posts: 390
Last seen: 4 years, 6 months
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Re: PE6 - Print -> Agar -> Transfer -> Grain -> Monotub [Re: mycomattie]
#18780691 - 08/31/13 09:20 AM (10 years, 4 months ago) |
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Quote:
mycomattie said: Once I felt I had some decent agar plates to work with (rhizo and contam free); I prepared quart jars of rye berries – pressure cooked for 90 minutes at 15psi and let cool overnight.
Im new to all this so forgive me if I'm misguided. Been doing a lot of reading up on working with agar/iso/LC. But don't you want the fastest growing rhizo sectors to isolate to get to a monoculture?
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: PE6 - Print -> Agar -> Transfer -> Grain -> Monotub [Re: TasteTheSound]
#18780751 - 08/31/13 09:49 AM (10 years, 4 months ago) |
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Quote:
TasteTheSound said:
Quote:
mycomattie said: Once I felt I had some decent agar plates to work with (rhizo and contam free); I prepared quart jars of rye berries – pressure cooked for 90 minutes at 15psi and let cool overnight.
Im new to all this so forgive me if I'm misguided. Been doing a lot of reading up on working with agar/iso/LC. But don't you want the fastest growing rhizo sectors to isolate to get to a monoculture?
Yup, that's what I <cough>attempted<cough> to do. When the spores germinated on the agar, I looked for the fastest growing, most rhizo/ropey sectors; and would transfer onto a new plate.
I'm new at this too! So that's how I approached it anyway....
In my mind, initially from a spore print, all you can do is isolate the most vigorous growing mycelium; since, at that point, you're not cloning or isolating large fruits, vigorous fruiter genetics; it's just MS - you don't know how it fruits at that point.
So in this grow, I felt I did isolate fast growing mycelium to the best of my new abilities; since in the photos above (8/31/13), it's been exactly 8 days since I initiated fruiting conditions.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: PE6 - Print -> Agar -> Transfer -> Grain -> Monotub [Re: TasteTheSound] 1
#18780754 - 08/31/13 09:51 AM (10 years, 4 months ago) |
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You don't need to isolate on agar to use it as inoculate. Its just an ms grow at that point, which you can clone from. I like to just do one or two transfers from the areas that show the best growth to ensure its clean and go from there. Then just take clones and see what you got.
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: PE6 - Print -> Agar -> Transfer -> Grain -> Monotub [Re: Pastywhyte]
#18780776 - 08/31/13 10:05 AM (10 years, 4 months ago) |
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Quote:
Pastywhyte said: You don't need to isolate on agar to use it as inoculate. Its just an ms grow at that point, which you can clone from. I like to just do one or two transfers from the areas that show the best growth to ensure its clean and go from there. Then just take clones and see what you got.
Cool man - thanks for the clarification!
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TasteTheSound
Cankle Lover


Registered: 08/05/12
Posts: 390
Last seen: 4 years, 6 months
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Re: PE6 - Print -> Agar -> Transfer -> Grain -> Monotub [Re: Pastywhyte]
#18782343 - 08/31/13 06:22 PM (10 years, 4 months ago) |
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Quote:
Pastywhyte said: You don't need to isolate on agar to use it as inoculate. Its just an ms grow at that point, which you can clone from. I like to just do one or two transfers from the areas that show the best growth to ensure its clean and go from there. Then just take clones and see what you got.
This is true for us beginners. I just spent the day reading the forums about isolations. And for us beginners the best way to start using agar is to make sure that we begin with a clean culture. We begin buy confirming there is no contaminates by growing MS on agar. No real need to isolate anything just make sure you have clean mycelium to do G2G transfers.
But depending on how far you actually want to take it, that's when agar really starts to shine. Yes its MS sample as of now and you have no idea what properties your grow will end up with. When Pasty said to just take clones and see what you got, what you got is a multi strain clone.
To truly get the most out of isolates you need to take several samples of what you believe are to be the best growing and continue to transfer these until you get a monoculture.
From what I read the amount of different strains you can sample is unlimited but you will have to grow each out to actually see what you got. Some of these monocultures may not even fruit. Some may flush better then others, be more potent, etc.
Then once you have isolated what you believe to be your star performing strain you grow it out and then take a clone from your best fruit. Then you will have a clone that is single strain and get those dope ass pinsets you see these old heads have.
Sorry for rambling on but I really find all this fascinating and writing/reiterating what I have read helps with the memory retention and helps spread the words to those who may not have the time to read or missed it on the search engine. Also others can help clarify any misunderstandings we may have.
So yes I'm hoping to use agar in the near future. One reason just to save my syringes til I get make my own spore prints. One day I hope to be able to isolate awesome strains that are kickass.
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mycomattie



Registered: 11/15/12
Posts: 1,323
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Re: PE6 - Print -> Agar -> Transfer -> Grain -> Monotub [Re: TasteTheSound] 1
#18783316 - 08/31/13 10:17 PM (10 years, 4 months ago) |
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Vis0nairee
Stranger



Registered: 12/02/13
Posts: 115
Last seen: 7 years, 8 months
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Re: PE6 - Print -> Agar -> Transfer -> Grain -> Monotub [Re: TasteTheSound]
#19338814 - 12/28/13 08:44 PM (10 years, 1 month ago) |
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Quote:
TasteTheSound said: This is true for us beginners. I just spent the day reading the forums about isolations. And for us beginners the best way to start using agar is to make sure that we begin with a clean culture. We begin buy confirming there is no contaminates by growing MS on agar. No real need to isolate anything just make sure you have clean mycelium to do G2G transfers.
But depending on how far you actually want to take it, that's when agar really starts to shine. Yes its MS sample as of now and you have no idea what properties your grow will end up with. When Pasty said to just take clones and see what you got, what you got is a multi strain clone.
To truly get the most out of isolates you need to take several samples of what you believe are to be the best growing and continue to transfer these until you get a monoculture.
From what I read the amount of different strains you can sample is unlimited but you will have to grow each out to actually see what you got. Some of these monocultures may not even fruit. Some may flush better then others, be more potent, etc.
Then once you have isolated what you believe to be your star performing strain you grow it out and then take a clone from your best fruit. Then you will have a clone that is single strain and get those dope ass pinsets you see these old heads have.
Sorry for rambling on but I really find all this fascinating and writing/reiterating what I have read helps with the memory retention and helps spread the words to those who may not have the time to read or missed it on the search engine. Also others can help clarify any misunderstandings we may have.
So yes I'm hoping to use agar in the near future. One reason just to save my syringes til I get make my own spore prints. One day I hope to be able to isolate awesome strains that are kickass.
Well put bro. Thanks!
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CMOS
Whats next?


Registered: 01/08/12
Posts: 833
Last seen: 9 years, 8 months
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Re: PE6 - Print -> Agar -> Transfer -> Grain -> Monotub [Re: Vis0nairee]
#19339923 - 12/29/13 01:32 AM (10 years, 1 month ago) |
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Oh was about to post back and saw that this was over 3 months old......
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