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Offlineblojo02184
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Re: Bucket Tek vs Pasteurization Thread [Re: TranscendingLife]
    #18781674 - 08/31/13 03:17 PM (10 years, 4 months ago)

How do you properly mix spawn and sub? In a still air tent? Cuz it can't fit in the sab


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OfflinePussyFart
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Re: Bucket Tek vs Pasteurization Thread [Re: blojo02184]
    #18781749 - 08/31/13 03:40 PM (10 years, 4 months ago)

Open air....

Pasteurized bulk substrate material is contam resistant.

So are fully colonized grains/cakes.


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InvisibleTranscendingLife
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Re: Bucket Tek vs Pasteurization Thread [Re: blojo02184]
    #18781828 - 08/31/13 04:05 PM (10 years, 4 months ago)

Quote:

blojo02184 said:
How do you properly mix spawn and sub? In a still air tent? Cuz it can't fit in the sab




2.Sterile Procedure:
    a.Turn off Heat/AC, Shut all windows & turn off all Fans.
    b.Oust Room
    c.Shower, wash hands, glove hands & wear a surgical mask.
    d.Oust once more
    e.Clean the inside of the tubs (make sure it dries before proceeding)
    i.I normally Unscrew the lids on my Spawn jars @ this time.


--------------------
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Offlineblojo02184
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Re: Bucket Tek vs Pasteurization Thread [Re: TranscendingLife]
    #18781905 - 08/31/13 04:22 PM (10 years, 4 months ago)

Thanks man


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InvisibleSpitballJedi
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Re: Bucket Tek vs Pasteurization Thread [Re: blojo02184]
    #18804899 - 09/05/13 08:02 PM (10 years, 4 months ago)

http://www.shroomery.org/forums/showflat.php/Number/17462182#17462182

Quote:

Citric said:
Quote:

Cynosure said:
I think most of my instances of Trichoderma in tubs came from contaminated spawn.  It seems that mushroom's mycelial growth occurs alongside the trich's which sometimes makes it hard for me to spot in jars.




Contaminated spawn would lead to bacteria.  Trich in your spawn would long show itself before the jar is 100% in most cases.  Trich would be from your substrate not being properly pasteurized.





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OfflineKizzle
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Re: Bucket Tek vs Pasteurization Thread [Re: SpitballJedi]
    #18805648 - 09/05/13 10:49 PM (10 years, 4 months ago)

Ever wonder why they sometimes add Trichoderma spores to coir?

It's because it's naturally sterile. There's virtually no bacteria or fungi on it to begin with.


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OfflineMush4Brains
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Re: Bucket Tek vs Pasteurization Thread [Re: Kizzle] * 1
    #18805660 - 09/05/13 10:52 PM (10 years, 4 months ago)

Trich helps several plant roots, I think.


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Invisibletbagtag
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Re: Bucket Tek vs Pasteurization Thread [Re: Mush4Brains]
    #18805730 - 09/05/13 11:11 PM (10 years, 4 months ago)

Quote:

Kizzle said:
Ever wonder why they sometimes add Trichoderma spores to coir?

It's because it's naturally sterile. There's virtually no bacteria or fungi on it to begin with.




and

Quote:

Mush4Brains said:
Trich helps several plant roots, I think.




Are both trueish....

That is the intention, but I've used trich treated coir for seedlings and hydroponic grows before i got into mycology.  The honest truth is - that it is more expensive by a few cents to a few dollars and generally the trich is dead by the time you get to use the brick.  I stopped buying it a long time ago and stuck with inert substances for plants. 

Off topic I know - but if you want good root growth germinate your seeds in damp towels or newspaper.  You can weed(pun intended) out your shit starter roots and keep the best looking ones.


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OfflineKizzle
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Re: Bucket Tek vs Pasteurization Thread [Re: Mush4Brains]
    #18805749 - 09/05/13 11:15 PM (10 years, 4 months ago)

But it isn't usually needed as an additive since practically all soil already has Trich in it.

The point is we pasteurize to kill molds while leaving some of the bacteria alive but if there's no native bacteria population then what exactly are we trying to leave alive? That's not to say there aren't other benefits to heat treating substrate


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Invisibletbagtag
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Re: Bucket Tek vs Pasteurization Thread [Re: Kizzle]
    #18805792 - 09/05/13 11:31 PM (10 years, 4 months ago)

Quote:

Kizzle said:
But it isn't usually needed as an additive since practically all soil already has Trich in it.

The point is we pasteurize to kill molds while leaving some of the bacteria alive but if there's no native bacteria population then what exactly are we trying to leave alive? That's not to say there aren't other benefits to heat treating substrate




Well we can never be sure that the coir was not exposed to bacteria at some point.  While its unlikely but so is finding dead bugs in bags of flour - anything can happen. I do it mostly for the mold issue, only had 1 tub go bacterial and that was my fault for dunking a mono for over 8 hours (got high forgot i dunked the fucker).


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Invisiblemaddchef
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Re: Bucket Tek vs Pasteurization Thread [Re: tbagtag]
    #18851491 - 09/16/13 09:48 PM (10 years, 4 months ago)

I still say the reason the bucket tek fails at times is because of the compression of the brick and uneven heating. I'm willing to bet if one were to hydrate it partially just to be able to expand it, then add the remaining water at 170 or so and seal it up for an hour, it would work just fine.

As far as cooling. After the 60-90 minutes, simply pour into your tub and toss a bit to throw off some heat.


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                                        Easier than cakes

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Offlineretaardvark
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Re: Bucket Tek vs Pasteurization Thread [Re: Kizzle]
    #18851757 - 09/16/13 10:49 PM (10 years, 4 months ago)

Quote:

Kizzle said:
But it isn't usually needed as an additive since practically all soil already has Trich in it.

The point is we pasteurize to kill molds while leaving some of the bacteria alive but if there's no native bacteria population then what exactly are we trying to leave alive? That's not to say there aren't other benefits to heat treating substrate




I read somewhere that the idea behind pasteurization is to allow thorough substrate colonization by moderately thermophilic organisms (those whose metabolisms are most active around 120~160°F). Surely there is quite a bit of environmental exposure to all sorts of organisms while hydrating and preparing a substrate for pasteurization, even if it was sterile when it was unwrapped.

I asked a few microbiologists and they said there was weight behind the idea, but could not confirm. Interested on experts' opinions!


Edited by retaardvark (09/16/13 10:56 PM)


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OfflineSoreSpore
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Re: Bucket Tek vs Pasteurization Thread [Re: PussyFart]
    #18851851 - 09/16/13 11:14 PM (10 years, 4 months ago)

Quote:

Notahacker420 said:
Pasteurized bulk substrate material is contam resistant.



Set it out in the open and let me know how contaminant resistant coir/coffee or ccm/verm mix :facepalm:


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Invisiblemaddchef
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Re: Bucket Tek vs Pasteurization Thread [Re: SoreSpore]
    #18851896 - 09/16/13 11:28 PM (10 years, 4 months ago)

If it weren't contam resistant, or at least slower to contam, why do all commercial farms pasteurize their compost and various subs. Sheesh


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

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OfflinePussyFart
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Re: Bucket Tek vs Pasteurization Thread [Re: SoreSpore]
    #18851936 - 09/16/13 11:41 PM (10 years, 4 months ago)

Quote:

SoreSpore said:
Quote:

Notahacker420 said:
Pasteurized bulk substrate material is contam resistant.



Set it out in the open and let me know how contaminant resistant coir/coffee or ccm/verm mix :facepalm:



No one is talking about adding coffee....but if properly pasteurized it should make little difference.

But still very contam resistant, considering if left out in the open it would most likely dry out before anything colonized it.

Plus people(including me) use coir all the time as reptile bedding....left in the tank for a month at a time with no mold growth.

Stop talking out of your ass, as you will get shut down quick round here'.


Edited by PussyFart (09/16/13 11:42 PM)


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OfflineMush4Brains
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Re: Bucket Tek vs Pasteurization Thread [Re: PussyFart]
    #18851955 - 09/16/13 11:47 PM (10 years, 4 months ago)

Quote:

Notahacker420 said:
No one is talking about adding coffee....but if properly pasteurized it should make little difference.

But still very contam resistant, considering if left out in the open it would most likely dry out before anything colonized it.

Plus people(including me) use coir all the time as reptile bedding....left in the tank for a month at a time with no mold growth.

Stop talking out of your ass, as you will get shut down quick round here'.




:thumbup:


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OfflineKizzle
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Re: Bucket Tek vs Pasteurization Thread [Re: SoreSpore]
    #18852005 - 09/17/13 12:06 AM (10 years, 4 months ago)

Quote:

Well we can never be sure that the coir was not exposed to bacteria at some point. 



I'm sure it has but that wouldn't mean much when the bacteria is unable to reproduce on it. Sterilizing most bulk substrates lead to an increase in contamination, despite the fact that bacteria is undoubtedly going start landing on the sterilized substrate right after sterilization.

That's because bacteria can only help prevent mold after there is a sufficient number of them.


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InvisibleFrankHorrigan
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Re: Bucket Tek vs Pasteurization Thread [Re: Kizzle]
    #18852011 - 09/17/13 12:09 AM (10 years, 4 months ago)

Quote:

SoreSpore said:
Quote:

Notahacker420 said:
Pasteurized bulk substrate material is contam resistant.



Set it out in the open and let me know how contaminant resistant coir/coffee or ccm/verm mix :facepalm:




Yeah, it'll just dry out, more than likely.

Maybe you should take a break from the pub and come learn something from mush cult. 7000+ posts and you still don't know how pasteurization works :lol:

Quote:

Well we can never be sure that the coir was not exposed to bacteria at some point. 




Bacteria is not a contaminant of pasteurized bulk substrate. 

If you have bacteria issues with properly pasteurized substrate, you can rest assured that it came from your spawn.


On bucket v proper p.

Bucket tek works a lot of the time. When it fails, it is out of your control (and nothing would have indicated that it failed until you saw the green).

It is not uncommon for people to blame the bucket tek when the culprit is their spawn.

Proper pasteurization eliminates one "what if" in the grand scheme of things, from spore to shroom. That's worth it to me.


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Re: Bucket Tek vs Pasteurization Thread [Re: SpitballJedi]
    #18853651 - 09/17/13 01:59 PM (10 years, 4 months ago)

Quote:

FrankHorrigan said:
It is not uncommon for people to blame the bucket tek when the culprit is their spawn.

Proper pasteurization eliminates one "what if" in the grand scheme of things, from spore to shroom. That's worth it to me.




Exactly.

Quote:

SpitballJedi said:
http://www.shroomery.org/forums/showflat.php/Number/17462182#17462182

Quote:

Citric said:
Quote:

Cynosure said:
I think most of my instances of Trichoderma in tubs came from contaminated spawn.  It seems that mushroom's mycelial growth occurs alongside the trich's which sometimes makes it hard for me to spot in jars.




Contaminated spawn would lead to bacteria.  Trich in your spawn would long show itself before the jar is 100% in most cases.  Trich would be from your substrate not being properly pasteurized.








This post by Citric makes since to me.

How long can trich stay hidden in a spawn jar?

I know trich looks similar to  cube myc until the trich turns green, but what about smell?

If it looks right and smells right, could it really have ninja trich? I mean really.


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OfflinePussyFart
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Re: Bucket Tek vs Pasteurization Thread [Re: SpitballJedi]
    #18853666 - 09/17/13 02:02 PM (10 years, 4 months ago)

Quote:

SpitballJedi said:
I know trich looks similar to  cube myc until the trich turns green, but what about smell?



The only think trich has in common with mushroom mycellium is the color white, but even the shade of white is different.

Trich is PURE white before it sporilates, the texture is different, it looks like mold, and it smells terrible.


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