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OfflineBlake_Shroom
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Iso looking weak on grain
    #18768257 - 08/28/13 12:32 PM (10 years, 5 months ago)

I dont know whats up. All my petris looked fantastic. The myc was so strong and amazing looking. Yet when I noc'd up some grain jars with agar wedges, I got some weak growth. All my previous jars with RGS had way stronger myc and when I tried to shake it, I barely could break up the grains. These grains separated easier than Rye.

I've since started some new MS cultures just in case this one sucks.

This culture is a clone from a tub I did in late june. I cloned to agar, cleaned it up, and made transfers to 20 plates. Once they were fully colonized, I threw them in the fridge for about a month since I was traveling. I got back 2 weeks ago, put them to grain, and this is whats happening...



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InvisibleFrankHorrigan
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Re: Iso looking weak on grain [Re: Blake_Shroom]
    #18768708 - 08/28/13 02:43 PM (10 years, 5 months ago)

I don't know man...those look fantastic to me :shrug:

Colonized WBS looks different than colonized RGS to be sure.


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InvisibleTranscendingLife
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Re: Iso looking weak on grain [Re: FrankHorrigan]
    #18769650 - 08/28/13 06:06 PM (10 years, 5 months ago)

:imwithstupid:


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
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OfflineBlake_Shroom
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Re: Iso looking weak on grain [Re: TranscendingLife]
    #18770303 - 08/28/13 08:16 PM (10 years, 5 months ago)

Hmm I guess it's hard to explain w/o seeing what all my other RGS jars looked like. Before this jar, I have never EVER been able to break apart all the grain while still in the jar. These ones were quite easy. Usually I do agar->grain jar->spawn bag so I can easy break it up while massaging it after a G2G.

There is a noticeably weaker growth pattern than before (this iso was derived from a MS grow and then a clone, so i've done many jars before with this culture before getting this iso).

Hopefully this next picture shows the weakness I feel like i'm seeing. Normally I wouldnt care, but I have 20+ qrt jars that I planned on G2G'ing into spawn bags and doing 20 tubs in september... I really hope this culture isnt too old



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OnlineStromriderM
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Re: Iso looking weak on grain [Re: Blake_Shroom]
    #18770956 - 08/28/13 10:11 PM (10 years, 5 months ago)

Looks good to me.
When I shake my fully colonized wbs jars the grains separate completely :shrug:


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InvisibleOeric McKenna
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Re: Iso looking weak on grain [Re: TranscendingLife]
    #18771156 - 08/28/13 11:09 PM (10 years, 5 months ago)

Quote:

TranscendingLife said:
:imwithstupid:



^How does this apply?

  & yeah man, myc takes on different appearances with different grains, as well as various moisture levels.  Mycelium in the fridge sometimes bums it out, so to speak. Especially with a tropical/sub-tropical species.


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OfflineBlake_Shroom
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Re: Iso looking weak on grain [Re: Stromrider]
    #18771265 - 08/28/13 11:37 PM (10 years, 5 months ago)

Quote:

psillyshroomer said:
Looks good to me.
When I shake my fully colonized wbs jars the grains separate completely :shrug:



As have mine. Same with Rye. But RGS was always a different story. I could break in into "chunks", but nothing like other grain when shaken.

Oh well, if this doesnt work out, at least I have another new culture started. And a big pan cyan project.

**** Just for clarification, I am comparing the strength of myc on RGS, not any other grain ****


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InvisibleOeric McKenna
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Re: Iso looking weak on grain [Re: Blake_Shroom]
    #18771429 - 08/29/13 12:30 AM (10 years, 5 months ago)

I've never grown on rye grass seed because its realy expensive (!)
I'm guessing its thicker, like a brf cake from what everyone's saying


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InvisibleTranscendingLife
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Re: Iso looking weak on grain [Re: Oeric McKenna]
    #18772700 - 08/29/13 11:40 AM (10 years, 5 months ago)

Quote:

Oeric McKenna said:
Quote:

TranscendingLife said:
:imwithstupid:



^How does this apply?

 




I agree with FH. That's how. :wow:


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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InvisibleOeric McKenna
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Re: Iso looking weak on grain [Re: TranscendingLife]
    #18773136 - 08/29/13 01:33 PM (10 years, 4 months ago)

Its the "stupid" part that is really condescending though. Not any more than the hammer to head icon I guess.
You're right, you're much more intelligent than me.


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InvisibleTranscendingLife
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Re: Iso looking weak on grain [Re: Oeric McKenna] * 1
    #18773234 - 08/29/13 01:55 PM (10 years, 4 months ago)

No need to be snarky.
You, obviously, can't take a joke. Also, you have no clue what mine & FH's relationship is.
Also, it's called a "wow" hammer & that's what I bring to all you serious internet people.
Hell, even RR has a sense of humour on the internet.
A lot of you "kids" need to figure out that internet is all jokes & sarcasm.
If not, then go :eatadick:


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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InvisibleOeric McKenna
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Re: Iso looking weak on grain [Re: TranscendingLife]
    #18773514 - 08/29/13 02:56 PM (10 years, 4 months ago)

Kids?  "Even RR HAS A SENSE OF HUMOR" Well we're all human & most have one...
You used to be really helpful to everyone but for the past 6 months you're just a condescending prick.
  I have nothing much against you but its pretty obvious
,
So I should go eat a dick now too huh? Cuz I'm serious, & a kid

  Sure.


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OnlineStromriderM
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Re: Iso looking weak on grain [Re: Oeric McKenna] * 1
    #18773522 - 08/29/13 02:58 PM (10 years, 4 months ago)

Seriously?


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InvisibleFrankHorrigan
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Re: Iso looking weak on grain [Re: Stromrider] * 1
    #18773933 - 08/29/13 04:26 PM (10 years, 4 months ago)

Whoa there. Breathe guys.

TL and me are on good terms.

He calls me stupid, I call him a girl :shrug:

No need to get all up in arms over nothing :thumbup:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OnlineStromriderM
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Re: Iso looking weak on grain [Re: FrankHorrigan]
    #18775802 - 08/30/13 01:09 AM (10 years, 4 months ago)

No kidding! Lighten up people. This is an Internet forum after all


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InvisibleTranscendingLife
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Re: Iso looking weak on grain [Re: Stromrider]
    #18776809 - 08/30/13 10:46 AM (10 years, 4 months ago)

:rofldrunk:

:commonsense:

Also, here's a list of my posts, I doubt you'll find more than 5 examples of me being a condescending prick.
https://www.shroomery.org/forums/dosearch.php?namebox=229579

How do you know I'm not a girl, FH :smirk:


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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InvisibleOeric McKenna
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Registered: 06/15/12
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Re: Iso looking weak on grain [Re: TranscendingLife]
    #18777020 - 08/30/13 11:42 AM (10 years, 4 months ago)

Hey thats the 5 examples! haha
Yeah & I'm sorry guys anyway. You know I love all you guys. (Girls? ha!)
    I've been killing it with nasty hours and short sleep. wage slave
Way too snappy for public use sometimes.
I apologize


--------------------


spread love
love is everything
2013 finds
medicinal psilocybin tincture drops
cannabis pics


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InvisibleTranscendingLife
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Re: Iso looking weak on grain [Re: Oeric McKenna]
    #18777043 - 08/30/13 11:48 AM (10 years, 4 months ago)

:hug:
We all go through our periods.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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InvisibleFrankHorrigan
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Re: Iso looking weak on grain [Re: TranscendingLife]
    #18777087 - 08/30/13 12:05 PM (10 years, 4 months ago)

Quote:

TranscendingLife said:
:hug:
We all go through our periods.




:whatshesaid:

Yeah, I'm a much more condescending prick sometimes, more than I think I've seen TL be.

Now excuse me, you ambiguously-gendered person, I just got hot pepper residue on my dick.

:onfire:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflineNakor420
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Re: Iso looking weak on grain [Re: FrankHorrigan]
    #18777124 - 08/30/13 12:14 PM (10 years, 4 months ago)

This is an entertaining thread....and those seed jars look amazing to me... :greatjob:


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