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Blake_Shroom
Stranger



Registered: 09/09/09
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Last seen: 1 year, 2 months
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Iso looking weak on grain
#18768257 - 08/28/13 12:32 PM (10 years, 5 months ago) |
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I dont know whats up. All my petris looked fantastic. The myc was so strong and amazing looking. Yet when I noc'd up some grain jars with agar wedges, I got some weak growth. All my previous jars with RGS had way stronger myc and when I tried to shake it, I barely could break up the grains. These grains separated easier than Rye.
I've since started some new MS cultures just in case this one sucks.
This culture is a clone from a tub I did in late june. I cloned to agar, cleaned it up, and made transfers to 20 plates. Once they were fully colonized, I threw them in the fridge for about a month since I was traveling. I got back 2 weeks ago, put them to grain, and this is whats happening...
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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I don't know man...those look fantastic to me 
Colonized WBS looks different than colonized RGS to be sure.
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Blake_Shroom
Stranger



Registered: 09/09/09
Posts: 1,070
Last seen: 1 year, 2 months
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Hmm I guess it's hard to explain w/o seeing what all my other RGS jars looked like. Before this jar, I have never EVER been able to break apart all the grain while still in the jar. These ones were quite easy. Usually I do agar->grain jar->spawn bag so I can easy break it up while massaging it after a G2G.
There is a noticeably weaker growth pattern than before (this iso was derived from a MS grow and then a clone, so i've done many jars before with this culture before getting this iso).
Hopefully this next picture shows the weakness I feel like i'm seeing. Normally I wouldnt care, but I have 20+ qrt jars that I planned on G2G'ing into spawn bags and doing 20 tubs in september... I really hope this culture isnt too old
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Stromrider
This must be the place


Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 9 minutes, 58 seconds
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Looks good to me. When I shake my fully colonized wbs jars the grains separate completely
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Oeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon
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Quote:
TranscendingLife said:

^How does this apply?
& yeah man, myc takes on different appearances with different grains, as well as various moisture levels. Mycelium in the fridge sometimes bums it out, so to speak. Especially with a tropical/sub-tropical species.
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Blake_Shroom
Stranger



Registered: 09/09/09
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Re: Iso looking weak on grain [Re: Stromrider]
#18771265 - 08/28/13 11:37 PM (10 years, 5 months ago) |
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Quote:
psillyshroomer said: Looks good to me. When I shake my fully colonized wbs jars the grains separate completely 
As have mine. Same with Rye. But RGS was always a different story. I could break in into "chunks", but nothing like other grain when shaken.
Oh well, if this doesnt work out, at least I have another new culture started. And a big pan cyan project.
**** Just for clarification, I am comparing the strength of myc on RGS, not any other grain ****
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Oeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon
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I've never grown on rye grass seed because its realy expensive (!) I'm guessing its thicker, like a brf cake from what everyone's saying
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Quote:
Oeric McKenna said:
Quote:
TranscendingLife said:

^How does this apply?
I agree with FH. That's how.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Oeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon
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Its the "stupid" part that is really condescending though. Not any more than the hammer to head icon I guess. You're right, you're much more intelligent than me.
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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No need to be snarky. You, obviously, can't take a joke. Also, you have no clue what mine & FH's relationship is. Also, it's called a "wow" hammer & that's what I bring to all you serious internet people. Hell, even RR has a sense of humour on the internet. A lot of you "kids" need to figure out that internet is all jokes & sarcasm. If not, then go
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Oeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon
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Kids? "Even RR HAS A SENSE OF HUMOR" Well we're all human & most have one... You used to be really helpful to everyone but for the past 6 months you're just a condescending prick. I have nothing much against you but its pretty obvious , So I should go eat a dick now too huh? Cuz I'm serious, & a kid
Sure.
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 9 minutes, 58 seconds
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Seriously?
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Iso looking weak on grain [Re: Stromrider] 1
#18773933 - 08/29/13 04:26 PM (10 years, 4 months ago) |
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Whoa there. Breathe guys.
TL and me are on good terms.
He calls me stupid, I call him a girl 
No need to get all up in arms over nothing
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 9 minutes, 58 seconds
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No kidding! Lighten up people. This is an Internet forum after all
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: Iso looking weak on grain [Re: Stromrider]
#18776809 - 08/30/13 10:46 AM (10 years, 4 months ago) |
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Also, here's a list of my posts, I doubt you'll find more than 5 examples of me being a condescending prick. https://www.shroomery.org/forums/dosearch.php?namebox=229579
How do you know I'm not a girl, FH
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Oeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon
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Hey thats the 5 examples! haha Yeah & I'm sorry guys anyway. You know I love all you guys. (Girls? ha!) I've been killing it with nasty hours and short sleep. wage slave Way too snappy for public use sometimes. I apologize
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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 We all go through our periods.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Quote:
TranscendingLife said:
 We all go through our periods.

Yeah, I'm a much more condescending prick sometimes, more than I think I've seen TL be.
Now excuse me, you ambiguously-gendered person, I just got hot pepper residue on my dick.
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Nakor420
Fun Guy



Registered: 05/02/13
Posts: 1,616
Loc: The Spirit World
Last seen: 9 years, 11 months
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This is an entertaining thread....and those seed jars look amazing to me...
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