Home | Community | Message Board

MushroomMan Mycology
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineJustsomedude
Stranger
Registered: 11/23/11
Posts: 29
Last seen: 9 years, 9 months
100% failure. Where am I going wrong?
    #18767558 - 08/28/13 08:24 AM (10 years, 5 months ago)

It's been a very long time since I posted but I've run into a major problem.  Right now 100% of my work is contaminated with green. It's been getting steadily worse over the last few months. I need some help determining where the contam is coming from.

Here is my procedure

Dunk jars and lids in 10% bleach solution for an hour
Rinse and clean wbs in bucket until water runs clear remove sunflower seeds
Fill add boiling water to bucket of clean seed and seal the lid
Let steep for an hour
Strain seed from boiling water onto large screens and let sit for 1 hour or until water stops draining
Scoop seed into pillow cases and massage to remove remaining water
Scoop thin layer of vermiculite into bottom of gallon jars
Scoop seed into jars until 3/4 full
Place metal lid with 2 pencil sized holes punched onto jar
Place synthetic filter disks over top of metal lid
Place and tighten metal mason jar ring
Cover top of jar with tin foil cap
Pressure cook at 15 psi for 90 minutes
Remove jars from pressure cooker and shake
Allow jars to cool
Unscrew mason jar ring slide filter disk back to reveal edge of punched hole
Insert needle of multispore syringe and inject 1 or 2 cc of solution in a swirl
Replace ring and tighten

How I made spore solution
Pressure cook water at 15 psi for 30 min allow to cool
Pressure cook a butter knife (spore scraping tool) in foil
Full syringes with water wrap in foil and pressure cook
Place cooked water spore print and other tools inside glove box
Clean inside of glove box with 99 isopropanol
Open tools and spore print
Open cooked water
Scrape sporeprint into clean water with tool
Seal spore solution and shake.
Attach brand new needles to syringes and draw solution
Seal small hole in spore solution jar with micropore tape and refrigerate
Any time I need to draw more syringes they get a brand new needle

Contam is happening in the jars. Does my procedure suck or is this just bad solution??


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,855
Last seen: 14 hours, 43 minutes
Trusted Cultivator
Re: 100% failure. Where am I going wrong? [Re: Justsomedude]
    #18767654 - 08/28/13 09:04 AM (10 years, 5 months ago)

The spores probably.

You should flame sterilize whatever you're transferring the spores with and it wouldn't hurt to flame the needles as well, even if they're new. There's a lot of opportunity for contamination between PCing a tool and using it.

Also I would only make one syringe at a time and limit the amount of spores for each syringe. More spores means more chances for contaminated spores and making multiple syringes from the same solution means if one gets contaminated they all do.


--------------------


Extras: Filter Print Post Top
OfflineJustsomedude
Stranger
Registered: 11/23/11
Posts: 29
Last seen: 9 years, 9 months
Re: 100% failure. Where am I going wrong? [Re: Kizzle]
    #18767703 - 08/28/13 09:21 AM (10 years, 5 months ago)

I was somewhat thinking the sporeprint maybe wasn't clean which in turn made bad solution. Previous solutions have been fine but the tail end of the last one seemed to contam things so I made a new batch. Since the new batch I've had complete failure. Guess I should redo that part.

Flame sterilization has to be until metal parts are literally red hot?


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,855
Last seen: 14 hours, 43 minutes
Trusted Cultivator
Re: 100% failure. Where am I going wrong? [Re: Justsomedude]
    #18767764 - 08/28/13 09:45 AM (10 years, 5 months ago)

You should> Then you'll know for sure they've reached a high enough temperature to guarantee they're sterile. That'd probably hard to with a butter knife. A paper clip would probably work better for transferring spores.


--------------------


Extras: Filter Print Post Top
OfflineJustsomedude
Stranger
Registered: 11/23/11
Posts: 29
Last seen: 9 years, 9 months
Re: 100% failure. Where am I going wrong? [Re: Kizzle]
    #18767890 - 08/28/13 10:18 AM (10 years, 5 months ago)

Will add flame to my procedure. I guess I'll have to switch to bleach to clean my glove box rather then the alcohol otherwise when I flame the needle and scraping tool after wiping the box down ill have a ball of fire in my living room.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* Sporeprint from a contaminated mushroom tomldp 2,046 2 10/10/01 09:40 AM
by shellacct
* Innoculation and contamination Metaxas 996 1 12/17/03 04:59 AM
by MAIA
* Contaminated casing - edibility..? (pics) BitchCakes 3,018 8 12/04/02 08:01 AM
by NeonBlack
* Catastrophic Failure!!!!!!!!!!! SafeHaven 1,084 8 03/03/03 02:28 AM
by SafeHaven
* Contamination Identification & Subjugation NutsackRucksack 13,842 4 11/19/20 09:38 PM
by Goat 2020
* Ecuadorian Contamination? Anonymous 1,863 15 10/14/03 05:12 PM
by Anonymous
* EXPERIMENT A FAILURE!?? agm4688 825 5 10/16/03 12:14 AM
by Ben_Stein
* Can contaminated spore water be saved? mesq 2,741 5 03/15/02 01:24 PM
by rungi

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
302 topic views. 41 members, 231 guests and 31 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.009 seconds on 14 queries.