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OfflineLoveLightPeace
Male


Registered: 08/26/11
Posts: 286
Last seen: 4 years, 11 months
Question about petri temp swings and condensation
    #18767265 - 08/28/13 05:39 AM (10 years, 5 months ago)

Ive heard that its a good idea to store colonized petri dishes in an insulated lunchbox and in the refrigerator, but wat about petri dishes that are still in the process of incubation and thus are kept outside the fridge at room temp, is it a good idea to also keep those ones stored in an insulated lunchbox as well?


--------------------
I am so small I can barely be seen.
How can this great love be inside me?

Look at your eyes. They are small,
but they see enormous things.

(Rumi)


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InvisibleUhurungus
Avoiderer of Bullshit
Male

Registered: 04/20/13
Posts: 838
Re: Question about petri temp swings and condensation [Re: LoveLightPeace]
    #18767473 - 08/28/13 07:56 AM (10 years, 5 months ago)

temp swings are the main cause of condensation inside dishes.  your dishes can be wrapped to cut down on contaminants with a breathable material, micropore tape or glad wrap, or a non breathable material, electrical tape.  the growth cycle of dishes is short enough that it's not a huge difference. 

by making sure the agar temp is cool enough when you pour you will cut down on initial temp swings and condensation cause from it.  by stacking the dishes, then putting the sleeve back over them and putting a cup of hot tap water on top of the stack for the cooldown period you will cut down on more condensation.  by not moving the dishes to different areas/temps during colonization you will cut down on even more condensation.

by doing the above things, i have zero problems with my dishes.


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I'm never 100% on anything, just close enough to risk looking foolish.


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Invisible1234go
Ban Lotto Champion
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Registered: 07/08/09
Posts: 53,857
Re: Question about petri temp swings and condensation [Re: Uhurungus]
    #18767488 - 08/28/13 08:03 AM (10 years, 5 months ago)

Quote:

Uhurungus said:
temp swings are the main cause of condensation inside dishes.  your dishes can be wrapped to cut down on contaminants with a breathable material, micropore tape or glad wrap, or a non breathable material, electrical tape.  the growth cycle of dishes is short enough that it's not a huge difference. 

by making sure the agar temp is cool enough when you pour you will cut down on initial temp swings and condensation cause from it.  by stacking the dishes, then putting the sleeve back over them and putting a cup of hot tap water on top of the stack for the cooldown period you will cut down on more condensation.  by not moving the dishes to different areas/temps during colonization you will cut down on even more condensation.

by doing the above things, i have zero problems with my dishes.




NICE info! Though i'm not the OP...THANKS.


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OfflineLoveLightPeace
Male


Registered: 08/26/11
Posts: 286
Last seen: 4 years, 11 months
Re: Question about petri temp swings and condensation [Re: 1234go]
    #18771720 - 08/29/13 03:13 AM (10 years, 5 months ago)

Thnx alot:)


--------------------
I am so small I can barely be seen.
How can this great love be inside me?

Look at your eyes. They are small,
but they see enormous things.

(Rumi)


Edited by LoveLightPeace (08/29/13 03:44 AM)


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