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lulz
Water Lover


Registered: 05/21/13
Posts: 217
Loc: Lost Woods
Last seen: 9 years, 8 months
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Why do you pasturize bulk substrate instead of steralize?
#18761850 - 08/26/13 10:44 PM (10 years, 5 months ago) |
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Just curious .
Edited by lulz (08/26/13 10:45 PM)
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MaJiK_420
...lost



Registered: 06/30/08
Posts: 447
Last seen: 6 years, 26 days
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Re: Why do you pasturize bulk substrate instead of steralize? [Re: lulz]
#18761884 - 08/26/13 10:53 PM (10 years, 5 months ago) |
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Google, search... something.
http://www.shroomery.org/5247/What-is-the-difference-between-pasteurization-and-sterilization-of-casings-and-substrates The pasteurization is carried out for a prolonged time at around 170°F and is supposed to kill only heat susceptible organisms and their spores, while sterilization works at a temperature of 250°F and is supposed to kill all organisms.
The pasteurization is carried out for a prolonged time at around 170°F and is supposed to kill only heat susceptible organisms and their spores, while sterilization works at a temperature of 250°F and is supposed to kill all organisms. The beneficial microorganisms(mainly bacteria)that are left alive when pasteurizing, guard the substrate against other contaminants, for instance different molds, but don't affect the growth of mushroom mycelium. Pasteurization is used for bulk substrates like straw, dung and composts and wood chips, also casings, and sterilization for all high nutritious substrates, like different grains.
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lulz
Water Lover


Registered: 05/21/13
Posts: 217
Loc: Lost Woods
Last seen: 9 years, 8 months
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Re: Why do you pasturize bulk substrate instead of steralize? [Re: MaJiK_420]
#18761953 - 08/26/13 11:10 PM (10 years, 5 months ago) |
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hey man that explains it thank you. i was just wondering why you wouldn't kill everything and you answered it for me.
P.S google likes to remember who you are if you search the right words.
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krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
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Re: Why do you pasturize bulk substrate instead of steralize? [Re: MaJiK_420]
#18761963 - 08/26/13 11:12 PM (10 years, 5 months ago) |
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Think of it as if you grew up in a clean room/laboratory from birth. If 20 years later you go outside you're going to be exposed to all kinds of foreign bacteria, viruses, and molds that your immune system/body is unprepared to deal with. Mushrooms don't exactly have 'immune systems' like humans do, but along with forming a symbiosis with beneficial bacteria (probiotics in the case of a human) mushrooms do create enzymes to help fight off and metabolize harmful, competing bacteria.
They quite possibly will be fine during the consolidation stage if you sterilized your substrate (however this is assuming you can somehow spawn it in an equally sterile environment), but then once you put it into fruiting conditions unless you again can somehow maintain this sterile environment, which is impractical for the home grower, then they will likely be overwhelmed.
So in a sense throughout the growing process you're slowly introducing more and more outside bacteria and competing molds/fungus (the beneficial bacteria help keep the contaminating molds/fungus at bay and they produce enzymes likewise that can fight those. Like majik mentioned pasturization selects for these bacteria).
If you could find a strain that could fight all of its competitors then we could just grow in the open air from the beginning, but not only would that take a lot of work I'm not sure if such a strain even exists so for the time being it's simply impractical.
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lulz
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Registered: 05/21/13
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Last seen: 9 years, 8 months
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Re: Why do you pasturize bulk substrate instead of steralize? [Re: lulz]
#18761972 - 08/26/13 11:15 PM (10 years, 5 months ago) |
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hmm makes perfect sense. thanks for shining the light guys.
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lulz
Water Lover


Registered: 05/21/13
Posts: 217
Loc: Lost Woods
Last seen: 9 years, 8 months
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Re: Why do you pasturize bulk substrate instead of steralize? [Re: lulz]
#18761988 - 08/26/13 11:18 PM (10 years, 5 months ago) |
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kinda like how the 'Native Americans' got fucked by European illnesses.
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krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
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Re: Why do you pasturize bulk substrate instead of steralize? [Re: lulz]
#18761999 - 08/26/13 11:23 PM (10 years, 5 months ago) |
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Exactly. Or how sometimes drinking the water in a foreign country will make you sick (except typically you live in such cases ).
Edited by krypto2000 (08/26/13 11:24 PM)
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lulz
Water Lover


Registered: 05/21/13
Posts: 217
Loc: Lost Woods
Last seen: 9 years, 8 months
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Re: Why do you pasturize bulk substrate instead of steralize? [Re: krypto2000]
#18762028 - 08/26/13 11:31 PM (10 years, 5 months ago) |
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o snap there goes my trip to Jamaica
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rockstaradam
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Registered: 08/13/12
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Re: Why do you pasturize bulk substrate instead of steralize? [Re: lulz]
#18763144 - 08/27/13 10:01 AM (10 years, 5 months ago) |
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Pastuerizing kills most of the bad organisms and leaves some of the beneficial organisms in the substrate. If you sterilized it, contams would over take that shit within a week
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Why do you pasturize bulk substrate instead of steralize? [Re: rockstaradam]
#18763178 - 08/27/13 10:11 AM (10 years, 5 months ago) |
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Any sterile media will contam in the presence of mold spores and bacteria.
Pasteurized substrate are already colonized with bacteria due to pasteurization.
Your live, healthy mycelium can rip through that.
But it will prevent mold spores from germinating and other (bad) bacteria from getting a foothold.
Since we spawn to substrate in open air, you can be assured there will be plenty of mold and bacteria mixed in your substrate.
Pasteurization is your first and only line of defense against that.
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