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Excel89
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Registered: 08/27/13
Posts: 21
Last seen: 10 years, 4 months
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Need help with spore prints that may or may not be contaminated
#18762301 - 08/27/13 01:50 AM (10 years, 5 months ago) |
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I haven't been into mushrooms in about a year. On my last batch about a year ago, I took 9 caps and made spore prints by putting small jars over them on top of a sheet of clear laminate paper. I never ended up properly storing them, they just sat in my attic for the last year with the jars over them. I'm now trying to start up again, and I'm trying to figure out if I can do anything with these prints. They were kept in the attic which was mostly dark (Though not completely), and the jars were over them which I'm hoping kept any air from getting it.
Right now I have 10 syringes that I sterilized and am waiting to cool. My plan is to use the prints to make spore syringes with distilled water, inoculate some jars with substrate, and see if they get contaminated. If they do get contaminated, would it be possible to remove a "clean" part of the mycelium, and grow it on agar?
Most of my experience has involved pre-bought syringes, and then after my first batch I usually make more syringes by flushing the sides of inoculated jars and sucking the spores back up. I don't have any experience with spore prints, making syringes, or agar besides what I've read.
If anyone can let me know if I have the right idea or not I'd appreciate it. With 9 prints (Some are dark and some are light), I'm assuming I can get about 30 or syringes, is that right? I'm about to do 10 right now to test, but depending on what you guys advise I may do all of them, or I may stop completely and go a different route.
Do you think the prints can be saved? What's my best option?
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llama_police
Fun guy



Registered: 02/20/13
Posts: 516
Loc: Australia
Last seen: 7 years, 11 months
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Re: Need help with spore prints that may or may not be contaminated [Re: Excel89]
#18762304 - 08/27/13 01:52 AM (10 years, 5 months ago) |
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Safest course of action would be agar
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Excel89
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Registered: 08/27/13
Posts: 21
Last seen: 10 years, 4 months
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Re: Need help with spore prints that may or may not be contaminated [Re: llama_police]
#18762313 - 08/27/13 01:56 AM (10 years, 5 months ago) |
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Should I put the spore print directly on agar or hydrate it first by putting it in the syringe? Think I should go ahead and do all of the syringes at once, and if so how much of each print should I use? When cultivating on agar do I use the whole syringe or just put a few spores? If I'm not using the whole syringe on the agar culture, should I wait and see if the agar is contaminated, and if not then use the syringe anyway? I've read a few teks but most of them explain what to do, but not what the process actually does or why to do it, so I'm still kind of lost.
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llama_police
Fun guy



Registered: 02/20/13
Posts: 516
Loc: Australia
Last seen: 7 years, 11 months
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Re: Need help with spore prints that may or may not be contaminated [Re: Excel89]
#18762459 - 08/27/13 03:20 AM (10 years, 5 months ago) |
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Make a few agar dishes/jars and try some with just a scraping of spores, then a few with a syringe made up from the print also. Basically, try a few different methods and see which ones work out best. The beauty of agar is that you can move healthy growth away from contamination and into a brand new agar dish
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 1 hour, 1 minute
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Re: Need help with spore prints that may or may not be contaminated [Re: llama_police]
#18762474 - 08/27/13 03:30 AM (10 years, 5 months ago) |
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Growing your mycelium out on agar and transferring away from contams as llama has said is your only option here. Those prints are most definitely full of mold spores. Research agar teks and I hope you are familiar with what cube mycelium looks like. We are talking about cubes right?
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Excel89
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Registered: 08/27/13
Posts: 21
Last seen: 10 years, 4 months
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Re: Need help with spore prints that may or may not be contaminated [Re: Stromrider]
#18767113 - 08/28/13 03:30 AM (10 years, 5 months ago) |
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Looks like I may be in a better position than I thought. I didn't realize I did this, but when I originally stored them, I actually put the spore print between two sheets of clear laminate paper, and put mason jars upside down on top of them in hopes that the weight would keep air from getting in. Not sure how well that would work, and again there was some indirect lighting it's been exposed to over the last year or so. I used the prints to make 10 petri dishes (Using tupperware containers that I disinfected), as well as 10 syringes. I saved one print in case I need it in the future.
Do you guys think that the spores will still be usable despite being a year old and exposed to a small amount of light over the last year? I'm keeping the dishes and syringes in a dark container right now and I'm going to see how look in a couple days.
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