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Offlinetr1pp1n
Stranger
Registered: 08/26/13
Posts: 3
Last seen: 10 years, 5 months
inoculate jars without glovebox and alcohol
    #18758684 - 08/26/13 07:17 AM (10 years, 5 months ago)

hey everyone on shroomery,

somebody who isnt me has a spore syringe , but does not own a auto-clave or glovebox to inoculate his presure cooked jars.

now the question:
can he inoculate open air without desinfection spray or alcohol? just burn the syringe needle with fire and then inoculate? how will be the contamination rate.

he does not want to waste his spore syringe and destroy it by contamination.

Using the PF-Tek , using 2 aluminium foils (not the lid-tek)

thanks for reading, and thanks for your answers.

tr1pp1n


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OfflineStromriderM
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Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say... Flag
Last seen: 11 hours, 45 minutes
Trusted Cultivator
Re: inoculate jars without glovebox and alcohol [Re: tr1pp1n] * 1
    #18758689 - 08/26/13 07:19 AM (10 years, 5 months ago)

Tell your friend to make an account and ask for himself

Also tell your friend to download the lets grow mushrooms video series


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Offlinetr1pp1n
Stranger
Registered: 08/26/13
Posts: 3
Last seen: 10 years, 5 months
Re: inoculate jars without glovebox and alcohol [Re: Stromrider]
    #18758693 - 08/26/13 07:24 AM (10 years, 5 months ago)

to make it clear, somebody who isnt me (SWIM) is often used in forums to make clear Im not doing any Illegal.

SWIM is registerd here.

dont be so rude, (sw)im getting started. :smile:

I just wonder if this syringe is wasted when using it without working sterile , got it from a vendor.


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OfflineStromriderM
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Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say... Flag
Last seen: 11 hours, 45 minutes
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Re: inoculate jars without glovebox and alcohol [Re: tr1pp1n]
    #18758704 - 08/26/13 07:32 AM (10 years, 5 months ago)

Not trying to be rude. Trying to help out bud! Watching that video is the best thing you can do right now. You can even watch the first 4 parts on youtube


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Offlineheratogwea
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Registered: 05/05/11
Posts: 607
Last seen: 3 years, 9 months
Re: inoculate jars without glovebox and alcohol [Re: tr1pp1n]
    #18758705 - 08/26/13 07:32 AM (10 years, 5 months ago)

Most people here will not answer swim...besides it does nothing for you.


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InvisibleK1ngSp4de
CHUT UP!!!
I'm a teapot User Gallery


Folding@home Statistics
Registered: 01/16/12
Posts: 1,680
Loc: Flag
Re: inoculate jars without glovebox and alcohol [Re: tr1pp1n]
    #18758707 - 08/26/13 07:32 AM (10 years, 5 months ago)

You're not fooling anyone but yourself using swim.

Get a SAB and some iso, should only be like $15 or so unless you can find a box from a friend, don't be cheap.


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OfflineStromriderM
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Re: inoculate jars without glovebox and alcohol [Re: Stromrider]
    #18758710 - 08/26/13 07:34 AM (10 years, 5 months ago)

And yes you can inoc in open air with flame sterilizing successfully :thumbup:

Now go watch the video and report back with your results :goodluck:


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OfflineBloodKil
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Registered: 03/16/13
Posts: 920
Last seen: 2 months, 9 days
Re: inoculate jars without glovebox and alcohol [Re: tr1pp1n]
    #18758720 - 08/26/13 07:44 AM (10 years, 5 months ago)

Quote:

tr1pp1n said:
to make it clear, somebody who isnt me (SWIM) is often used in forums to make clear Im not doing any Illegal.

SWIM is registerd here.

dont be so rude, (sw)im getting started. :smile:

I just wonder if this syringe is wasted when using it without working sterile , got it from a vendor.




To make it clear...  Your a fucking retard if you think saying swim will somehow help you avoid prosecution if they were to somhow build a case on you.  Aside from that it's annoying as shit (as is saying your pets, etc.. are doing it)

Your chance of getting in trouble for asking a question on cultivation is nil.  But if your that overly paranoid, there is a simple solution...  Don't participate in illegal activities.

All that said I wish you the best of luck in growing your shrooms...  Just drop the swim bs and you will get a lot more help.


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Offlinemiw4life
The Shroomer
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Registered: 07/04/13
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Re: inoculate jars without glovebox and alcohol [Re: tr1pp1n]
    #18758721 - 08/26/13 07:45 AM (10 years, 5 months ago)

hey bud, to answer your question. yes you can inoculate successfully without the spray or glovebox. although it is not recommended. ive done a couple of my last jars without them. and they are fully colonized now. so if it need be to do the procedure without them just sterilize that needle after every hole just to be safe.


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"The answer to every question, is the question itself"


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Offlinetr1pp1n
Stranger
Registered: 08/26/13
Posts: 3
Last seen: 10 years, 5 months
Re: inoculate jars without glovebox and alcohol [Re: miw4life]
    #18758869 - 08/26/13 08:51 AM (10 years, 5 months ago)

hey thanks for all that answers, I think youre right.

Im new to this community, I read alot about mushrooms and watch videos about it, but in every serious video it says you have to work clean else you will get contamination - worse case scenario for a newbie that want to be succesful.


thats why I thought I should wait with starting and gather all the tools and materials I need.

I mean I have:
PC
JARS
VERM.
BROWN RICE
S. SYRINGE

Dont have:
GLOVE BOX
ALCOHOL (95%)
GLOVES (STERILE ONES)

:laugh:


Quote:

miw4life said:
hey bud, to answer your question. yes you can inoculate successfully without the spray or glovebox. although it is not recommended. ive done a couple of my last jars without them. and they are fully colonized now. so if it need be to do the procedure without them just sterilize that needle after every hole just to be safe.




thanks for that answer!

I just gonna try it , it seems like its trial and error in the beginning. Or starting of with perfect tools and materials you need.


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Invisiblegrainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
Re: inoculate jars without glovebox and alcohol [Re: tr1pp1n]
    #18759080 - 08/26/13 10:02 AM (10 years, 5 months ago)

Go buy a bottle of 70% ISO and a roll of paper towels.  It'll cost $5.


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OfflineStratman
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Registered: 06/30/11
Posts: 294
Loc: At the centre of infinity
Last seen: 1 year, 4 months
Re: inoculate jars without glovebox and alcohol [Re: grainbrain]
    #18759313 - 08/26/13 11:31 AM (10 years, 5 months ago)

Just cheap, clean rubber gloves rubbed with alcohol are fine. When the alcohol dries your gloves become sterile enough.
For a SAB, you can use a cardboard box with Saran Wrap over the top. Just get creative with the equipment you have. Don't risk wasting your spores through bad methods.


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Offlineganjfather
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Registered: 08/06/09
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Re: inoculate jars without glovebox and alcohol [Re: Stratman]
    #18759517 - 08/26/13 12:52 PM (10 years, 5 months ago)

I've innoc'd plenty of times in open air, but I always use alcohol to swab down the inoculation point and I flame sterilize. It can be done, but I would at least go buy some alcohol. You can get a bottle for a dollar.


I'd recommend just buying a tub and building a SAB. It's not that hard, a tub costs you maybe 10. Get a knife and cut out arm holes. Viola, a still air box.


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Re: inoculate jars without glovebox and alcohol [Re: Stratman]
    #18759681 - 08/26/13 01:51 PM (10 years, 5 months ago)

Quote:

Stratman said:
For a SAB, you can use a cardboard box with Saran Wrap over the top. Just get creative with the equipment you have. Don't risk wasting your spores through bad methods.




This. Equipment can be substituted if you absolutely have to. Proper technique cannot.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Offlined9c6
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Registered: 07/02/13
Posts: 632
Last seen: 7 years, 5 months
Re: inoculate jars without glovebox and alcohol [Re: 36fuckin5]
    #18759814 - 08/26/13 02:33 PM (10 years, 5 months ago)

Alcohol is very cheap. A tote is around 10-15$


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OfflineStromriderM
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Re: inoculate jars without glovebox and alcohol [Re: tr1pp1n]
    #18760047 - 08/26/13 03:29 PM (10 years, 5 months ago)




Buy 70% alcohol :thumbup:


Edited by Stromrider (08/26/13 03:30 PM)


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OfflineLingabhakt
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Registered: 08/17/13
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Re: inoculate jars without glovebox and alcohol [Re: Stromrider]
    #18760205 - 08/26/13 03:56 PM (10 years, 5 months ago)

If you have corn, you have alcohol. :smile: If you dont have corn, you are an ET & maybe they even have corn. Hell, its easier to make alcohol than it is to grow shrooms. SAB is also easy & cheap. My 9 yo son does all this with his oysters (little fucker loves the little fuckers). I understand being frugal, but don't be cheap. You will only let yourself down. As for the SWIM BS, there is this funny part of the law called conspiracy to (enter crime here). If SWIM commits a crime & you help him (such as by seeking out information to help facilitate a crime), you are just as guilty as SWIM. I have a buddy who is serving time right now because he gave an encryption program to a "friend" who turned out to be an avid fan of kiddie porn. Because they had a text from the perv saying he wanted to to protect his "secret porn stash" the DA argued that my buddy knew what that porn was. It being a bench trial, the judge found for the DA. Now my buddy is labeled a perv by society.


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I'm not real, my posts aren't real & reality is really unreal.
OM NAMAH SHIVAAYA!
TAT TVAM ASI!
OM SHANTIH SHANTIH SHANTIH...


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InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
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Re: inoculate jars without glovebox and alcohol [Re: Lingabhakt]
    #18760261 - 08/26/13 04:08 PM (10 years, 5 months ago)


:whippedcreamhead:

Nice crazy-person rant :lol:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


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InvisiblePestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
Re: inoculate jars without glovebox and alcohol [Re: Lingabhakt]
    #18760325 - 08/26/13 04:22 PM (10 years, 5 months ago)

That was very entertaining! :thumbup:


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OfflineStratman
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Registered: 06/30/11
Posts: 294
Loc: At the centre of infinity
Last seen: 1 year, 4 months
Re: inoculate jars without glovebox and alcohol [Re: Pestile]
    #18760523 - 08/26/13 05:06 PM (10 years, 5 months ago)

:goodluckwiththat2:


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Shop: Mushroom-Hut Liquid Cultures   North Spore Cultivation Supplies   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Capsules


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