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Offlinekrypto2000
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Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
I just realized there are three tiny holes on the bottom of one end of my nearly colonized mono...
    #18759963 - 08/26/13 03:10 PM (10 years, 5 months ago)

It's nearly fully colonized, and no real signs of it being unhealthy other than an increase of metabolites lately which I previously did not think was due to contamination, but now am unsure. The metabolites did not appear until I increased the amount of light and moved it to another room figuring it would help speed up colonization and help prepare it for birth. Clearly these holes are leaving it open to contamination though. If you're wondering why they're there I was doing an experiment with growing a monotub outside, I tilted the bin slightly so that I could water it like a plant and then it would drain out of these holes. Apparently I accidentally reused this for a standard monotub project without realizing it until now.

Ime the metabolites are simply due to being too warm atm, but there is also some metabolites beginning to pool at the bottom, at least of another bin next to it (this other bin is clear so I can see, the one in question though I had painted the outside black). There is no smell what so ever coming from the three holes, if anything it smells like clean/pasturized hpoo so again I have little reason to believe the mushies are having much of a problem with bacterial infections, however also those 3 sections seem like they might have dried out and be hard to colonize for them (this is not necessarily a bad thing as it's essentially created its own filter patch, however the filter patch it likely full of nutrients being hpoo, but there is white mycilial growth around it which is also dried up so perhaps this growth is or has leached what nutrients it can from it).

So obviously my question is simply what should I do about this? I have a few ideas, but having never dealth with something like this nothing stands out as a best or clear cut solution.

1.) Spray H2O2 on it and seal it with masking tape. I could also tape some felt to it (I don't have micropore tape as this would obviously be the best 'bandaid').  edit: Actually upon checking I don't have any H202, so I'd have to make an oxyclean/sodium per carbonate solution as that's all I have available).

2.) Put some salt on it and tape it up (Salt kills bacteria and mushrooms can likewise metabolise/'eat' it). I also have some epsom salts which I can put on it, however the epsom salts have been kept in the bathroom and being hydroscopic have grabbed things from the air, I'm not sure of the properties of them, I know they're antibacterial, but they might actually introduce contaminant spores into the bin this way, they are however much more easily metabolized by mushrooms being primarily magnesium and sulphur as opposed to primarily sodium as table salt is, so I'm not sure which is a better option).

3.) This probably isn't a good solution, but I feel it should be mentioned, I could simply leave it alone.


Edited by krypto2000 (08/26/13 03:12 PM)


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Offlinekrypto2000
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Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
Re: I just realized there are three tiny holes on the bottom of one end of my nearly colonized mono... [Re: krypto2000]
    #18760086 - 08/26/13 03:38 PM (10 years, 5 months ago)

I could also plug up the holes with pellitized hydrated lime, but that depends on whether the bacteria beneficial to mycilium are acid loving or not, I assume they are not since mushrooms likewise are a rather acid loving species, but that doesn't necessarily mean the beneficial bacteria that they symbiot with are as well. Help and advice would be much appreciated on this. I've been cleaning/dusting all day so my house is likely full of mold spores/endospores atm, and likewise I've allowed the temperature to rise upwards of 80f to help with this process only to discover these unfortunate holes. For the time being I've wiped the surrounding area with alcohol to clean it careful not to actually touch the holes and am dropping the temp in the house to give it more time in which I can hopefully come up with a good solution here.


Edited by krypto2000 (08/26/13 03:42 PM)


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OfflineMush4Brains
LOOL HACKED!!!

Registered: 07/31/13
Posts: 4,419
Last seen: 9 years, 2 months
Re: I just realized there are three tiny holes on the bottom of one end of my nearly colonized mono... [Re: krypto2000]
    #18760141 - 08/26/13 03:46 PM (10 years, 5 months ago)

If it's nearly fully colonized just let it be :shrug:


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Offlinekrypto2000
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Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
Re: I just realized there are three tiny holes on the bottom of one end of my nearly colonized mono... [Re: Mush4Brains]
    #18760425 - 08/26/13 04:42 PM (10 years, 5 months ago)

What about after birth though, will the holes not likely have an effect on fruiting? I wonder even if they might be beneficial, hmm...


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Invisibletoxetel
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Registered: 08/13/12
Posts: 708
Re: I just realized there are three tiny holes on the bottom of one end of my nearly colonized mono... [Re: krypto2000]
    #18760479 - 08/26/13 04:53 PM (10 years, 5 months ago)

What do you mean when you say "birth?"

To second what Mush4Brains said, I wouldn't worry about it. When I spawn to bulk in plastic deli containers I poke holes in the lid for gas exchange anyway. If you really want to you can tape the holes up now. But I don't imagine you sterilized the manure and performed the spawn-to-bulk in sterile conditions, so there were always contaminants present in some quantity. Fully colonized grains and properly pasteurized bulk substrate is resistant to contamination.


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Offlinekrypto2000
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Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
Re: I just realized there are three tiny holes on the bottom of one end of my nearly colonized mono... [Re: krypto2000]
    #18760867 - 08/26/13 06:35 PM (10 years, 5 months ago)

These holes are in the very bottom, but yeah you're probably right. They're definitely small enough that if anything, along with the heat generated by the mycilium, they'd create an upward current. It's obviously a small window for contaminants, but likewise they're so small I can't, and don't, see it being an issue. The current might also aid in pinning and for allowing more total moisture/water if I were to mist the bin, theoretically this could even allow for bigger mushrooms, or at least taller skinnier ones, that would all depend upon the humidity and nutrient availability. This also gives a whole new meaning to "myc piss' lol, as they may not have a bladder, but they now do have a pee hole, or three :rofl:. By birth I simply meant initiate fruting, I guess 'birth' is more akin for the pf-tek, but same basic principles.


Edited by krypto2000 (08/26/13 06:39 PM)


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