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joonkun
Stranger


Registered: 08/10/13
Posts: 16
Last seen: 10 years, 2 months
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how long before myc growth.
#18759747 - 08/26/13 02:13 PM (10 years, 5 months ago) |
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i know theres several threads that delve into this, but im just looking on advice on my particular case. bought b+, using BRF tek, from spore syringe, been about 5-6 days i'd say and one syringe has decent growth but the other 3 have 0 growth, i mean 0, like no mold, no nothing literally the jar looks like i just took it out of the pressure cooker. Just curious how long should i wait before saying those cakes are trash? its just strange, because it doesnt seem as if they are contam, cause theres 0 growth of anysort, contam or not. and one syringe, which came from the same batch, has some decent growth. Any suggestions? should i wait? or are those cakes jsut bad? i know its been less then a week and spore takes longer then agar or LC or something, but its weird one syringe would show growth but the others not. the cakes were PC'd together, and theres no contams growing so i dont think its something with my sterile procedures. any advice?
Edited by joonkun (08/26/13 05:58 PM)
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hankstymcbankerson

Registered: 07/24/13
Posts: 180
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Re: how long before myc growth. [Re: joonkun]
#18759792 - 08/26/13 02:28 PM (10 years, 5 months ago) |
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The spores in the quick-to-colonize jar were just sluts.
In my extremely novice opinion, Id say as long as they (the non-growth showing jars) dont smell sour or funky, let em keep going for a few-several more days before pulling the plug. Spores might just be a little shy.
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joonkun
Stranger


Registered: 08/10/13
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Last seen: 10 years, 2 months
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yeah i wouldnt say they smell funny at all, im hoping they will pull through. when i got them all the syringes looked good, none of them seemed empty or anything, i have no idea whats going on, i would understand maybe if they got some contam growing, but after 5-6 days i'd expect if A.) i had contam for it to show up by now, by something growing or smell or something, and since all the cakes were mixed/pc together. It's just odd to me that 1 syringe is looking good and the other 3 are showing absolutely nothing, is it possible that something happen during shipping and 1 syringe managed to pull through but the others were compromised or something?
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Fryer Mike
Lost in the fourth dimension.


Registered: 02/22/09
Posts: 426
Last seen: 1 year, 3 months
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Re: how long before myc growth. [Re: joonkun]
#18759924 - 08/26/13 03:02 PM (10 years, 5 months ago) |
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I have done several grows. Being in Ontario, it's not really very humid or hot most of the time, even during the summer. I have seen jars begin to colonize as late as 3 weeks.
How long did you wait after sterilization before inoculation?
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DON'T READ THIS! Any information/ pictures/ text that implies I grow or use illegal substances is false and/ or for entertainment purposes. Nothing I say or submit to this forum or to any other should be taken literally. I am become death, destroyer of worlds. Fryer Mike
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joonkun
Stranger


Registered: 08/10/13
Posts: 16
Last seen: 10 years, 2 months
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Re: how long before myc growth. [Re: Fryer Mike]
#18759938 - 08/26/13 03:05 PM (10 years, 5 months ago) |
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i finished pressue cooking around 2am, and i started to inoc around 2pm the next day, so around 12 hours, during this time the lid was never opened.
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Fryer Mike
Lost in the fourth dimension.


Registered: 02/22/09
Posts: 426
Last seen: 1 year, 3 months
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Re: how long before myc growth. [Re: joonkun]
#18759950 - 08/26/13 03:08 PM (10 years, 5 months ago) |
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Alright. I was worried that maybe you inoculated to soon and the jars were too hot, but that's obviously not the case.
I would say wait until between 10 and 20 days after inoculation, and then consider re-inoculation.
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DON'T READ THIS! Any information/ pictures/ text that implies I grow or use illegal substances is false and/ or for entertainment purposes. Nothing I say or submit to this forum or to any other should be taken literally. I am become death, destroyer of worlds. Fryer Mike
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joonkun
Stranger


Registered: 08/10/13
Posts: 16
Last seen: 10 years, 2 months
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Re: how long before myc growth. [Re: Fryer Mike]
#18759975 - 08/26/13 03:12 PM (10 years, 5 months ago) |
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well resanitizing and reinoc is a solution if nothing grows, but im also trying to pinpoint why this might have happened? bad syringes? bad tek ect? i just dont want to waste more syringes before i know where i went wrong, or if something was out of my control / bad luck. more or less at 16$ a syringe, and 3 of them seemingly at this point going nowhere, i kinda wanna figure out whats up.
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Fryer Mike
Lost in the fourth dimension.


Registered: 02/22/09
Posts: 426
Last seen: 1 year, 3 months
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Re: how long before myc growth. [Re: joonkun]
#18760001 - 08/26/13 03:18 PM (10 years, 5 months ago) |
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Well my novice opinion is that either you haven't waited long enough to be able to make a solid decision on what the issue may be. They still might begin to colonize. What is the temperature in your grow area? Is it consistent?
If they just don't colonize I would say the likely cause would be bad syringes.
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DON'T READ THIS! Any information/ pictures/ text that implies I grow or use illegal substances is false and/ or for entertainment purposes. Nothing I say or submit to this forum or to any other should be taken literally. I am become death, destroyer of worlds. Fryer Mike
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joonkun
Stranger


Registered: 08/10/13
Posts: 16
Last seen: 10 years, 2 months
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Re: how long before myc growth. [Re: Fryer Mike]
#18760200 - 08/26/13 03:55 PM (10 years, 5 months ago) |
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yeah its consistant, they are inside, so the temperature isnt much of a factor, i have central air, and therefore the temp stays pretty stable with little flucations, 76-78 degrees F is where my thermastat is set. they are near a window, not in the direct light, like off to the side, but get the same light as natural.
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brianstequila
Sir John Falstaff



Registered: 05/20/13
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Re: how long before myc growth. [Re: joonkun]
#18760656 - 08/26/13 05:42 PM (10 years, 5 months ago) |
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You have to have patience andremove the sponsers name from your post, it is against the rules and they only sell for microscopic uses only.
-------------------- Every citizen should be a soldier. This was the case with the Greeks and Romans, and must be that of every free state. Thomas Jefferson Not My trade list i aint got shit anymore
http://www.shroomery.org/forums/showflat.php/Number/19161913
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joonkun
Stranger


Registered: 08/10/13
Posts: 16
Last seen: 10 years, 2 months
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i removed the name, however i dont see how any of what ive said was "against" the rules, theres tons of posts in the sponser section which state a.) they have bought products with the intent to grow them, and B.) the specific vendor in which they have done the transaction with. im not sure how their post is any different from mine?
Additonally, i am being patient, im not jumping up and down complaining how its taking so long, im trying to figure out why one syringe would colonize possibly say 3-5% and the other syringes have 0 growth at all, when everything was done with the same procedure, same batch of cake mix, same pressure cook, same sterile tek ect, ect, only thing different between the groups are the syringes. im not asking for someone to say do this and you will have fruit tomorrow, i am however looking for why A.) the cakes are now bad or B.) why such a massive differential in growth, for curiosity, knowlege, and future use.
Edited by joonkun (08/26/13 06:05 PM)
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BloodKil
Mangler av era mödrar slida


Registered: 03/16/13
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Re: how long before myc growth. [Re: joonkun]
#18760871 - 08/26/13 06:35 PM (10 years, 5 months ago) |
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Hello different spores from the same syringe might vary from a few days to a few weeks to germinate... Just sit back, drink a few beers and keep waiting. It's quite possibly just the genetics, and unless your jars start to stink or show miscolored growth, there is nothing else to be done for a while.
As far as the rules... (vendors name in conjunction with cultivation) take time to read them, and just because someone else does something wrong, it doesn't make it okay for you.
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: how long before myc growth. [Re: joonkun]
#18761585 - 08/26/13 09:42 PM (10 years, 5 months ago) |
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Quote:
joonkun said: i know theres several threads that delve into this, but im just looking on advice on my particular case.
Yes, there are literally thousands of threads with this same question. You're growing cubes from spores just like all of those other threads. Whats different about your particular case?
Nothing, that's what. Patience grasshoppa
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Quick WBS Prep
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joonkun
Stranger


Registered: 08/10/13
Posts: 16
Last seen: 10 years, 2 months
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Re: how long before myc growth. [Re: FooMan]
#18763440 - 08/27/13 11:24 AM (10 years, 5 months ago) |
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first off theres no need for a childish condescending attitude. secondly if you actually read through you would see what you are saying DOES NOT fit what i have said, im not asking how long it takes spores to grow, im asking if theres a reason that 1 syringe EVERY jar has growth, while the 3 other syringes HAVE NO GROWTH, contams or other. im not asking why some jars are growing faster or how long it takes to grow or any of that which are covered in other threads.The fact is 1 set of jars is growing, with some variance as aspected with multi spore but all show some sort of growth, but not 1 out of 38 jars from the remainder 3 syringes show one scrap of contams, growth, or sour/musty smell. ALL jars were mixed together from same batch, p/c'd together and same exact steps in sterile tek followed. a few jars i could understand, but not a single jar from 3 different syringes? sounds awfully unlikely from a statistical standpoint that i wouldnt atleast get a jar or 2 to show atleast a little growth. while it is a question on multi spore inoculation the circumstances ARE VERY different in my thread. so before you feel the need to act as if you are being helpful, or even useful, you might want to look through a thread because you not only look like a jerk, but have not offered one scrap of advice or information.
Edited by joonkun (08/27/13 11:26 AM)
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: how long before myc growth. [Re: joonkun]
#18764239 - 08/27/13 02:41 PM (10 years, 5 months ago) |
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Calm down bro! It was a joke, hence the smirk. You're not getting far with that attitude and you're right- There aren't thousands of other threads asking why one strain or syringe is outperforming others or why "X" syringe has growth when "Y" and "Z" syringes didn't. silly me 
You had already received some helpful answers. I'm just reminding you that the search feature will provide you with 99.999% of your answers if you put some time into actually searching.
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Quick WBS Prep
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