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ShroomPuncher
The FunGuy



Registered: 10/30/12
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Psilocybe Cubensis B+ (First grow)
#18759563 - 08/26/13 01:09 PM (10 years, 5 months ago) |
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I'm gonna start my log here. For some reason, it won't let me post in the actual grow log forum. Please, criticize the shit out of me if necessary. I don't wanna ruin my first grow. All suggestions are welcome.
8/26
Woke up, re-watched the BRF videos, re-read some useful info and decided to start.
Even though it's not necessary, I used Lysol wipes to sanitize the part of the kitchen I'd be using. Punched holes in the lid jars (1/2 pint, wide mouth), and doing it wrong, some of them dented in a little which concerned me about getting leaks when steam sterilizing. I'll just wrap extra good with aluminum foil.
Made the substrate. 2 cups verm, 1 cup water. Mixed. Added 1 cup BRF. Mixed. A little chunky but the chunks break easy. Filled 4 jars, wiped moisture / substrate off the 1/2 inch or so off the top of the jar. Everything's not perfectly level. I'm sure this will be fine? 
Ran out of substrate. I'm gonna mix a little bit more and add a couple jars to the mix out of the extra I have. Maybe 6-7 total jars.
Getting back to work. Will update soon while sterilizing. Going to elevate the jars approx. 1/2 inch from the water (using lids and foil), then boil for 90 minutes, checking for steam ever so often.
Jars filled up
 
I ran out of lids and found some old ones but the tops seemed to have mold or rust? The rings themselves though had nothing on them. Still, I'm boiling them then wiping them with alcohol before putting them in the pot to sterilize with the jars. I'm Howard Hughes up in here. Nothing can get too clean.

6:02 PM- 90 minutes is up for steam sterilizing. Added an extra 15 to make up for all the times I checked the lid. Only added extra water once. Going to let cool until tomorrow morning and then sterilize the workspace and continue with inoculations.
8/27
9:33 AM- Woke up and about to start cleaning.
12:00P PM - Got done inoculating jars. Did 2 outside SAB and the rest inside. Hoping everything was sterile enough. Now to wait.
9/6
Jars had a nasty odor and dark spots in some of them. Only 1 has any signs of growth. I've moved the 6 smelly jars elsewhere. 3 in one room, 3 in another.
 
9/7
The one jar seems to be slowly moving along. The temp is lower than it should be so I'ma try to get a heater in here.

9/8
The jar I have the most faith in now has a orange-ish spot, but still seems to be developing well and pretty fast.
 
Here's a pic of one of the smelly jars. They're all still separated from ol' faithful but I still don't have my hopes high.

All the other smelly ones are coming along like this one.
9/9
I'm sterilizing more jars. The other jars seem to be the same. Not much growth of whatever is growing in there.


Hopefully the 2 (possibly 3) I shoot up tomorrow will have better results. If not, I'm pretty sure it's the syringe.
Edited by ShroomPuncher (09/09/13 10:52 AM)
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tlien


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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18759589 - 08/26/13 01:20 PM (10 years, 5 months ago) |
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Sounds good, bro.
Keeping things clean is always a good idea. You never know where mold spores can sneak their way into your jars from.
And I wouldn't worry about your substrate filter layer not being level, mushrooms don't care too much heh.
Yeah, everything sounds in order here.
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Kjetterfaen
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18759647 - 08/26/13 01:42 PM (10 years, 5 months ago) |
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18759790 - 08/26/13 02:27 PM (10 years, 5 months ago) |
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Boiling now. I appreciate the feedback! My 1 concern is that they may get waterlogged. I covered a few of them (7 total) with extra foil and made sure the foil is on tight. I used a 2:1:1 ratio of verm/water/BRF. Think I'll be good? Probably just paranoid.
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d9c6



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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher] 1
#18759819 - 08/26/13 02:35 PM (10 years, 5 months ago) |
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All the luck in the world to ya!
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tlien


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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18759856 - 08/26/13 02:44 PM (10 years, 5 months ago) |
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That is the correct ratio, yeah!
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DevilFromRs
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Re: Psilocybe Cubensis B+ (First grow) [Re: tlien]
#18759969 - 08/26/13 03:11 PM (10 years, 5 months ago) |
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Diggin the purple oven light. Good start, you can never be too careful going about a sanitized working area for your jars/grow. I also am starting a b+ strain grow via pf tek so I am interested in watching your grow.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: DevilFromRs]
#18760014 - 08/26/13 03:21 PM (10 years, 5 months ago) |
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Quote:
DevilFromRs said: Diggin the purple oven light. Good start, you can never be too careful going about a sanitized working area for your jars/grow. I also am starting a b+ strain grow via pf tek so I am interested in watching your grow.
That's just how it turned out in the camera haha. But from what I've read, B+ colonizes rather quickly and is more contam resistant... or something else that indicated that it was pretty noob-friendly. Hopefully you can learn from my mistakes.... Or maybe I'll have a clean grow.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18760233 - 08/26/13 04:01 PM (10 years, 5 months ago) |
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Steam sterilized for 90 minutes. Gonna go ahead and tack on another 15 minutes to make up for all the probably unnecessary times I've lifted the lid. That, and I just want to be safe.
I'm gonna wake up early tomorrow and start sterilizing my workspace and the area around it (Lyson air spray, wiping counters with 70% iso alcohol, mopping, etc.). I have a still air box but it's not comfortable and I want to be able to see what I'm doing for my first inoculations so I can get an idea of the right amount and whatnot. I'll shut all the windows, keep animals in separate rooms, and be wearing a dust or paint mask, tyvec sleeves, and nitrile gloves, which will all be sterilized.
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KnownPown
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18762190 - 08/27/13 12:39 AM (10 years, 5 months ago) |
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My first grow was 8 B+ jars and I was tripping on dry shrooms on the 1 month birthday of when I inoculated the jars
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: KnownPown]
#18762770 - 08/27/13 07:31 AM (10 years, 5 months ago) |
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Quote:
KnownPown said: My first grow was 8 B+ jars and I was tripping on dry shrooms on the 1 month birthday of when I inoculated the jars 
Nice! How much did you produce on your first grow?
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18763159 - 08/27/13 10:05 AM (10 years, 5 months ago) |
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Inoculation is done. I'm a little disappointed. I lysol'ed the house after vacuuming, sweeping, then mopping the kitchen I'm working in. I then wiped all the counters and most everything else with alcohol.
I started the first two jars without an air box. Noticing that the flame on the lighter was flickering more than I liked, I decided to use the air box, which I cleaned beforehand with bleach Lysol and disinfected a little with alcohol.
My problems are that 1, my lighters went out so I lit a candle and the candle made my needle tip black, which I know some residue from that got into a couple of the jars (Which I wiped BEFORE and AFTER inoculation with alcohol).
Other than that, my hands were a little shaky, I probably didn't get one jar (or hole for that matter) perfect with 1 CC (or 1/4 per hole, although using 7 jars, I have a little under 3 CC remaining in a 10 CC syringe.
Also, when I was heating the needle, whether with a lighter or candle, it kind of spitting out spore solution? I'm assuming this is normal since liquid and fire have that reaction? Also, I flame sterilized the needle for a few seconds in each part until it was red (except right against the syringe since I wanted to avoid burning the syringe plastic itself), but the entire needle was never all at once red hot, just the parts the lighter touched until I moved it along to a different spot.
So, basically my concerns:
-Air wasn't 100% still so I used my ghetto SAB after 2 jars -Candle made needle black on a couple of occasions. -The entire needle was at not point completely red hot. Rather, sections were red hot at a time but I feel like it would've killed any contams that would've landed on it?
Thoughts? 
Also, other than possibly adding more heat, does light speed up colonization at all or is light not a factor?
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KnownPown
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18768551 - 08/28/13 01:51 PM (10 years, 5 months ago) |
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My first grow was 8 half pint B+ Jars and I just recently threw them out after they grew some cobweb mold after 2nd flush. I harvested about 1.5-2 oz dry total from all flushes off those 8 cakes.
Currently fruiting out the rest of my 8 EQ and 8 GT cakes, GT have given me a great even pinset first flush, B+ gave me a pretty good yield and EQ had few but massive fruits, still averaged 3g dry or 30g wet per cake per flush.
Looks like you did things like I did pretty much, I just inoculated in my ghetto SAB in my closet and used alcohol and a lighter for my first time. Lighter seemed to work fine. I later just put osme alcohol in a small dish so my needle didnt get all nasty.
Goodluck man!
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: KnownPown]
#18768656 - 08/28/13 02:25 PM (10 years, 5 months ago) |
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Quote:
KnownPown said: My first grow was 8 half pint B+ Jars and I just recently threw them out after they grew some cobweb mold after 2nd flush. I harvested about 1.5-2 oz dry total from all flushes off those 8 cakes.
Currently fruiting out the rest of my 8 EQ and 8 GT cakes, GT have given me a great even pinset first flush, B+ gave me a pretty good yield and EQ had few but massive fruits, still averaged 3g dry or 30g wet per cake per flush.
Looks like you did things like I did pretty much, I just inoculated in my ghetto SAB in my closet and used alcohol and a lighter for my first time. Lighter seemed to work fine. I later just put osme alcohol in a small dish so my needle didnt get all nasty.
Goodluck man!
Nice, I wanna give Golden Teachers a try next. Just the name alone is worth the grow. Good to see you had a good grow. Hope I do as well.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18781412 - 08/31/13 01:43 PM (10 years, 4 months ago) |
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Update: 4 days- no signs of colonization or contamination. Temps have probably been about 72 (least) to 79 (most). Hoping for something soon.
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tlien


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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18781504 - 08/31/13 02:18 PM (10 years, 4 months ago) |
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You're making me mad, OP. Did you remember to aim the syringes towards the edge of the glass? That way, you will see the growth the earliest.
Goddamn, I'm so mad right now. What did your syringes look like? Did you see tiny blobs in it?
SO MAD!
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Stromrider
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Re: Psilocybe Cubensis B+ (First grow) [Re: tlien]
#18781519 - 08/31/13 02:24 PM (10 years, 4 months ago) |
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5 days is the earliest I ever see growth. Sometimes it takes 8 to 10
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Stromrider]
#18781723 - 08/31/13 03:34 PM (10 years, 4 months ago) |
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Quote:
tlien said: You're making me mad, OP. Did you remember to aim the syringes towards the edge of the glass? That way, you will see the growth the earliest.
Goddamn, I'm so mad right now. What did your syringes look like? Did you see tiny blobs in it?
SO MAD!
Yup, aimed towards the edges and watched it drip down. Syringes were pretty damn dark but from a reputable vendor. And there were clumps but I shook it up pretty well.
Quote:
psillyshroomer said: 5 days is the earliest I ever see growth. Sometimes it takes 8 to 10
That seems like good time. I'll keep that in mind. I have a feeling mine will take a little longer though.
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tlien


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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18781953 - 08/31/13 04:33 PM (10 years, 4 months ago) |
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Dark is good, my man. That means there are numerous spores in there.
I'm only speaking from experience of TWO syringes, which I made myself, one had no visible spores, 3/3 jars survives, with regular growth, one was filled with dark blotches, 6/6 survived, rapid growth.
I hope your jars colonize well
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: tlien]
#18782028 - 08/31/13 04:54 PM (10 years, 4 months ago) |
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Quote:
tlien said: Dark is good, my man. That means there are numerous spores in there.
I'm only speaking from experience of TWO syringes, which I made myself, one had no visible spores, 3/3 jars survives, with regular growth, one was filled with dark blotches, 6/6 survived, rapid growth.
I hope your jars colonize well 
Thanks for the reassurance! I'm hoping they come along some time soon.
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WhiteSide
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18782066 - 08/31/13 05:05 PM (10 years, 4 months ago) |
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Im growing B+ too! But only six jars. Glad to see someone else growing the same
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Stromrider
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Re: Psilocybe Cubensis B+ (First grow) [Re: WhiteSide]
#18782070 - 08/31/13 05:06 PM (10 years, 4 months ago) |
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I'm growing cubes too
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shadow6211
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Re: Psilocybe Cubensis B+ (First grow) [Re: Stromrider]
#18796492 - 09/03/13 09:13 PM (10 years, 4 months ago) |
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Any updates on the jars Shroompuncher? I'm growing B+ for my first time also and was wondering how yours were going.
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ShroomPuncher
The FunGuy



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Re: Psilocybe Cubensis B+ (First grow) [Re: shadow6211]
#18798944 - 09/04/13 12:02 PM (10 years, 4 months ago) |
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Still no growth.
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Kjetterfaen
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18798972 - 09/04/13 12:08 PM (10 years, 4 months ago) |
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Really? No growth on any of the jars? What day are you at now?
But hey, at least there is ONE positive about the "no growth" situation... It doesn't seem like you have a contam problem at least.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18798994 - 09/04/13 12:16 PM (10 years, 4 months ago) |
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Quote:
Kjetterfaen said: Really? No growth on any of the jars? What day are you at now?
But hey, at least there is ONE positive about the "no growth" situation... It doesn't seem like you have a contam problem at least.
It's been 8 days I believe. And yeah, I'm glad for the lack of contams. I'm thinking maybe it's a bad syringe . I'm gonna try to knock up a couple more jars tomorrow with the rest of the 2-3ish CCs I have left and see what happens. I thought surely I'd see a sign of something by now.
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Kjetterfaen
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18799387 - 09/04/13 02:09 PM (10 years, 4 months ago) |
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Is these the only syringes you have as well? I mean, this is your first go so you only have x amount of syringes from the same supplier?
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PussyFart
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18799394 - 09/04/13 02:13 PM (10 years, 4 months ago) |
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Spores can take up to 3 weeks to germinate and show growth....be patient....
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: PussyFart]
#18799567 - 09/04/13 02:52 PM (10 years, 4 months ago) |
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Quote:
Kjetterfaen said: Is these the only syringes you have as well? I mean, this is your first go so you only have x amount of syringes from the same supplier?
Yup, 1 syringe from the same supplier.
Quote:
Notahacker420 said: Spores can take up to 3 weeks to germinate and show growth....be patient....
I know. Patience isn't my thing but I know it's required for growing. On a side note, if I had any contams from sterilization procedures, would they have likely shown by now?
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PussyFart
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18799578 - 09/04/13 02:55 PM (10 years, 4 months ago) |
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Yes.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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shadow6211
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Re: Psilocybe Cubensis B+ (First grow) [Re: PussyFart]
#18804694 - 09/05/13 07:07 PM (10 years, 4 months ago) |
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Shroompuncher give me an update on your jars im curious!
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: shadow6211]
#18806245 - 09/06/13 05:20 AM (10 years, 4 months ago) |
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In the past couple days, I've noticed some darker spots in my jars. At first they were very subtle but now they're more noticeable. They basically just look like wet, dark spots on the substrate but there's no way they've gained any more moisture.
Here's the only clear pic I could get but it's on a couple jars. They're not really a different color than the substrate.. just darker.

Signs of mycelial growth?
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Kjetterfaen
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18806496 - 09/06/13 08:16 AM (10 years, 4 months ago) |
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The dark areas sound scary to me, but I'm as much a beginner as you are so that's more or less the extent of what I can offer on the subject.
But yeah, congrats, you got myc in that last pic. Let us hope it's the right kind. =)
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tlien


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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18807245 - 09/06/13 12:14 PM (10 years, 4 months ago) |
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Quote:
ShroomPuncher said: In the past couple days, I've noticed some darker spots in my jars. At first they were very subtle but now they're more noticeable. They basically just look like wet, dark spots on the substrate but there's no way they've gained any more moisture.
Here's the only clear pic I could get but it's on a couple jars. They're not really a different color than the substrate.. just darker.

Signs of mycelial growth?

Get hold of a magnifier glass, get a cheap but powerful one. If you look at the growth, and you see strands all radiating from the point of origin like the hub of a bicycle and the spokes, it's mycelium. You might be able to see it without tools as well.
Mycelium grows that way, mold grows chaotically.
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heratogwea



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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18807442 - 09/06/13 01:18 PM (10 years, 4 months ago) |
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Quote:
ShroomPuncher said: In the past couple days, I've noticed some darker spots in my jars. At first they were very subtle but now they're more noticeable. They basically just look like wet, dark spots on the substrate but there's no way they've gained any more moisture.
Here's the only clear pic I could get but it's on a couple jars. They're not really a different color than the substrate.. just darker.

Signs of mycelial growth?

I had an amazon syringe that did that to six of my jars. Made the sub darker. I thought maybe I used too much innoculant so I let them sit a few days..with no change. Took a whiff and almost gagged.
I would say those are no good ime.
The jars with the white I would say are deff myc.. atleast that's how mine looked when it started
Edited by heratogwea (09/06/13 01:22 PM)
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shadow6211
Brimlow



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Re: Psilocybe Cubensis B+ (First grow) [Re: heratogwea]
#18807715 - 09/06/13 02:43 PM (10 years, 4 months ago) |
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Damn man same thing is happening with mine they are exactly the same looking hopefully they grow i guess we will see!
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heratogwea



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Re: Psilocybe Cubensis B+ (First grow) [Re: shadow6211]
#18807755 - 09/06/13 03:00 PM (10 years, 4 months ago) |
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Give em a good whiff
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: heratogwea]
#18807898 - 09/06/13 03:48 PM (10 years, 4 months ago) |
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The ones with the dark spots smell pretty sour. I forgot about smelling the jars. I'm gonna move them into a completely different location in case somehow my smell is off or they recover, which I'm not counting on. And the one with white growing must be mycelium because it's fits all the characteristics you guys have listed above. Even the one jar with myc smells a little funny but I have allergies and I don't know exactly what I'm smelling for so I'm gonna keep my eye on it. I'll keep you guys posted!
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tlien


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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18810558 - 09/07/13 05:15 AM (10 years, 4 months ago) |
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I don't think smelling the jars is a good idea, because I assume it involves opening the lids, which exposes them to contamination.
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PussyFart
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Re: Psilocybe Cubensis B+ (First grow) [Re: tlien]
#18810563 - 09/07/13 05:19 AM (10 years, 4 months ago) |
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Quote:
tlien said: I don't think smelling the jars is a good idea, because I assume it involves opening the lids, which exposes them to contamination.
Well you don't open them to smell until they are fully colonized.
In some cases you can smell it thru the holes in the lid.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: PussyFart]
#18811279 - 09/07/13 11:40 AM (10 years, 4 months ago) |
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Quote:
Notahacker420 said:
Quote:
tlien said: I don't think smelling the jars is a good idea, because I assume it involves opening the lids, which exposes them to contamination.
Well you don't open them to smell until they are fully colonized.
In some cases you can smell it thru the holes in the lid.
Which is what I did and even then, it smells overwhelmingly nasty. The one is growing still but has a little bit of a sour smell. Not gonna count it out yet and I'm gonna shoot up some more Monday but with a blank this time and maybe catch where I screwed up this time.
When sterilizing bad jars, do you steam sterilize them in the same way you do after packing the substrate?
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18811502 - 09/07/13 12:51 PM (10 years, 4 months ago) |
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Looking healthy?

compared to yesterday, it's growing a little.
Here's yesterday
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18811534 - 09/07/13 01:01 PM (10 years, 4 months ago) |
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Quote:
ShroomPuncher said: I'm gonna start my log here. For some reason, it won't let me post in the actual grow log forum. Please, criticize the shit out of me if necessary. I don't wanna ruin my first grow. All suggestions are welcome.
8/26
Woke up, re-watched the BRF videos, re-read some useful info and decided to start.
Even though it's not necessary, I used Lysol wipes to sanitize the part of the kitchen I'd be using. Punched holes in the lid jars (1/2 pint, wide mouth), and doing it wrong, some of them dented in a little which concerned me about getting leaks when steam sterilizing. I'll just wrap extra good with aluminum foil.
Made the substrate. 2 cups verm, 1 cup water. Mixed. Added 1 cup BRF. Mixed. A little chunky but the chunks break easy. Filled 4 jars, wiped moisture / substrate off the 1/2 inch or so off the top of the jar. Everything's not perfectly level. I'm sure this will be fine? 
Ran out of substrate. I'm gonna mix a little bit more and add a couple jars to the mix out of the extra I have. Maybe 6-7 total jars.
Getting back to work. Will update soon while sterilizing. Going to elevate the jars approx. 1/2 inch from the water (using lids and foil), then boil for 90 minutes, checking for steam ever so often.
Jars filled up
 
I ran out of lids and found some old ones but the tops seemed to have mold or rust? The rings themselves though had nothing on them. Still, I'm boiling them then wiping them with alcohol before putting them in the pot to sterilize with the jars. I'm Howard Hughes up in here. Nothing can get too clean.

6:02 PM- 90 minutes is up for steam sterilizing. Added an extra 15 to make up for all the times I checked the lid. Only added extra water once. Going to let cool until tomorrow morning and then sterilize the workspace and continue with inoculations.
8/27
9:33 AM- Woke up and about to start cleaning.
12:00P PM - Got done inoculating jars. Did 2 outside SAB and the rest inside. Hoping everything was sterile enough. Now to wait.
9/6
Jars had a nasty odor and dark spots in some of them. Only 1 has any signs of growth. I've moved the 6 smelly jars elsewhere. 3 in one room, 3 in another.
 
9/7
The one jar seems to be slowly moving along. The temp is lower than it should be so I'ma try to get a heater in here.

Bookmarked for future reference as I'm planning on attempting to grow maybe B+ soon, hope it turns out for the best dude
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tlien


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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18812067 - 09/07/13 04:03 PM (10 years, 4 months ago) |
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Quote:
ShroomPuncher said: Looking healthy?

compared to yesterday, it's growing a little.
Here's yesterday

Like I said, it's hard to tell from your photos, what you need to do is get a magnifier glass and look at the growth.
If it's chaotic, it's mold. If it's radiating outwards from a point, like the spokes of a bicycle wheel, it's mycelium.
I can't tell from the pics.
Perhaps you can tell if you get real close and just see for yourself.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: tlien]
#18812416 - 09/07/13 06:04 PM (10 years, 4 months ago) |
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I think I have a contam in it anyways. From what you described, the white growth is mycelium but I've noticed a very subtle, light orangeish spot near the top of the jar right below the dry vermiculite.
I don't see how I could have 100% failure? 6 not colonizing w/ nasty odor and dry spots and 1 with another possible contam.
Checking back on the six vectors of contamination in Paul Stamets' book.
1) The cultivator - I showered, brushed my teeth, and wore nitrile gloves, tyvec sleeves, and a dust mask, all of which I rubbed 70% iso alcohol on.
2) The air - I used lyson disinfectant spray pretty heavily and multiple times in the area.
3) The media - I steam sterilized the substrate for a little over 90 minutes. The moisture content wasn't altered but I did open the lid more times than necessary.. I don't think that's the issue, though.
4) The tools - I iso'ed everything then flame sterilized the needle between jars. Like I said before, the needle wasn't consistently red hot, but I feel like anything would've been destroyed. I used a paper towel and wiped the needle between jars, but BEFORE flame sterilizing.
5) The inoculum - This is why I should've set up a blank jar to see if it got contaminated without even being inoculated. Maybe it was a bad syringe? I got it from a reputable vendor (shroomery sponsor)
6) Mobile contamination units - Nothing / no one entered the work area during inoculation procedures.
So judging by that, I have no clue. Maybe I didn't light the syringe enough? Maybe it was the syringe or paranoid sterilization that led me to fiddle with stuff to much and over-clean? I'll post an update again of the jars tomorrow. 2 of the ones I moved have small, mycelium-like dots that have recently developed but due to the odor, I'm not getting excited. Probably just a mold.
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Kjetterfaen
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18812740 - 09/07/13 07:38 PM (10 years, 4 months ago) |
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Seeing as it is two different (likely) contams, maybe the one near the vermiculite barrier was caused by the verm barrier being moved around during the time you impatiently awaited growth? The other ones seem to be right on the inoculation spots, so I guess those were probably introduced by the needle or the spore solution..
No idea where the contam was picked up in the first place; you seem to have been pretty thourough... with the exception of the lack of SAB. Do you live in a particularly mold-prone house or area? Any factors like that?
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18812784 - 09/07/13 07:50 PM (10 years, 4 months ago) |
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Quote:
Kjetterfaen said: Seeing as it is two different (likely) contams, maybe the one near the vermiculite barrier was caused by the verm barrier being moved around during the time you impatiently awaited growth? The other ones seem to be right on the inoculation spots, so I guess those were probably introduced by the needle or the spore solution..
No idea where the contam was picked up in the first place; you seem to have been pretty thourough... with the exception of the lack of SAB. Do you live in a particularly mold-prone house or area? Any factors like that?
I forgot to say that I did use an SAB, which I thoroughly cleaned beforehand with bleach and then swabbed the arm holes with alcohol. And there is mold in my house but the area I inoculated wasn't quite that bad. I mean I sterilized the hell out of the room but Monday, I'm gonna use the rest of my spores and nocc up 2 more jars but this time include a blank and see what happens.
Has anyone here ever had sour smelling jars that they managed to salvage? I'm seeing growth in a few of them. It's white, but I'm not sure if it's the white I'm looking for.
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PussyFart
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18812795 - 09/07/13 07:53 PM (10 years, 4 months ago) |
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Quote:
ShroomPuncher said: I mean I sterilized the hell out of the room
No you didn't, that would be impossible.
Sanitized maybe....but not sterilized....not that anything needs to be sterile, or even sanitary for that matter when working in a SAB....it just has to provide a still enough environment so that all the mold in the world would not matter.
Better sterile technique is needed my son....
Quote:
ShroomPuncher said: Has anyone here ever had sour smelling jars that they managed to salvage?
No...
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Kjetterfaen
Strangler

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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18812808 - 09/07/13 07:55 PM (10 years, 4 months ago) |
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Even if possible I wouldn't risk trying to salvage it as a beginner myself. I mean, we don't even know what specifically is wrong with them.. I suppose you could try fruiting them outside somewhere?
Now this might be a stupid suggestion, but there isn't any chance you can knock up a jar at someone elses place is there? I'm thinking eliminate your house entirely and see what the result is..?
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PussyFart
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18812823 - 09/07/13 07:59 PM (10 years, 4 months ago) |
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It should not matter where he knocks up the jars.....hell open air is fine if he is quick...
Flame the needle until red hot, then exponge a drop or 2 to cool the needle, then immediately inoculate....simple....
Do this in a SAB for maximum safety....not the flaming part of course...do that outside the box...
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Kjetterfaen
Strangler

Registered: 07/21/13
Posts: 495
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Re: Psilocybe Cubensis B+ (First grow) [Re: PussyFart]
#18812860 - 09/07/13 08:09 PM (10 years, 4 months ago) |
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Just thinking "one less factor" 
But yeah, the steps Hacker listed there is more or less what I followed myself. Add to that some normal soap and water action (showered and made sure my house was relatively clean (which was sorely needed anyway)) and the gloves and mask and you got most of my precautions.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: PussyFart]
#18812912 - 09/07/13 08:26 PM (10 years, 4 months ago) |
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Quote:
Notahacker420 said:
Quote:
ShroomPuncher said: I mean I sterilized the hell out of the room
No you didn't, that would be impossible.
Sanitized maybe....but not sterilized....not that anything needs to be sterile, or even sanitary for that matter when working in a SAB....it just has to provide a still enough environment so that all the mold in the world would not matter.
Better sterile technique is needed my son....
Quote:
ShroomPuncher said: Has anyone here ever had sour smelling jars that they managed to salvage?
No...
Well, then I sanitized the hell out of it. And I'm thinking it was the needle. I just had a few moments where I wasn't as confident as the other procedures. I'm definitely gonna use my SAB again but find another way to flame sterilize my needle rather than holding a lighter with my shaky ass hands. And I like the recommendation of letting a few drops go out before inoculating. Last time, I'm assuming from the heat, you could hear the spore water spurting and every now and then it would shoot a little water out.
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Kjetterfaen
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18812927 - 09/07/13 08:34 PM (10 years, 4 months ago) |
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Hey, on that note I want to tack on a question... I have heard using butane flames are inadvisable, and that only clean burning alcohol should be used. Why is this? Is it all down to sooty residue or is there some other reason? Is it even a real concern?
Personally I use an alcohol lamp and denatured alcohol (cheap and easy, and possible improvise if one can't be procured otherwise), you might want to give that a shot ShroomPuncher - for some more controlled flaming, regardless of the butane/alcohol thing.
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heratogwea



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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18812946 - 09/07/13 08:38 PM (10 years, 4 months ago) |
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Quote:
Kjetterfaen said: Hey, on that note I want to tack on a question... I have heard using butane flames are inadvisable, and that only clean burning alcohol should be used. Why is this? Is it all down to sooty residue or is there some other reason? Is it even a real concern?
Personally I use an alcohol lamp and denatured alcohol (cheap and easy, and possible improvise if one can't be procured otherwise), you might want to give that a shot ShroomPuncher - for some more controlled flaming, regardless of the butane/alcohol thing.
I used a bic lighter and had no problems  I also wiped with alcohol after each burn.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: heratogwea]
#18812949 - 09/07/13 08:40 PM (10 years, 4 months ago) |
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Good thought, Kjetterfaen. I'm gonna look into making a 'survival alcohol lamp'. I think my problem was the way I was using the flame. A little inconsistent, slow, etc. But I definitely got the needle pretty dirty when I started using a candle.
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Kjetterfaen
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18812955 - 09/07/13 08:43 PM (10 years, 4 months ago) |
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Mmm, glyceryl ester.
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tlien


Registered: 10/07/12
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18814037 - 09/08/13 04:31 AM (10 years, 4 months ago) |
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Wow, you went to great lengths trying to avoid this... hmm.
Perhaps the contents of the syringe was contaminated?
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Sockadin



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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18814156 - 09/08/13 07:14 AM (10 years, 4 months ago) |
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Quote:
ShroomPuncher said: I'm gonna start my log here. For some reason, it won't let me post in the actual grow log forum. Please, criticize the shit out of me if necessary. I don't wanna ruin my first grow. All suggestions are welcome.
8/26
Woke up, re-watched the BRF videos, re-read some useful info and decided to start.
Even though it's not necessary, I used Lysol wipes to sanitize the part of the kitchen I'd be using. Punched holes in the lid jars (1/2 pint, wide mouth), and doing it wrong, some of them dented in a little which concerned me about getting leaks when steam sterilizing. I'll just wrap extra good with aluminum foil.
Made the substrate. 2 cups verm, 1 cup water. Mixed. Added 1 cup BRF. Mixed. A little chunky but the chunks break easy. Filled 4 jars, wiped moisture / substrate off the 1/2 inch or so off the top of the jar. Everything's not perfectly level. I'm sure this will be fine? 
Ran out of substrate. I'm gonna mix a little bit more and add a couple jars to the mix out of the extra I have. Maybe 6-7 total jars.
Getting back to work. Will update soon while sterilizing. Going to elevate the jars approx. 1/2 inch from the water (using lids and foil), then boil for 90 minutes, checking for steam ever so often.
Jars filled up
 
I ran out of lids and found some old ones but the tops seemed to have mold or rust? The rings themselves though had nothing on them. Still, I'm boiling them then wiping them with alcohol before putting them in the pot to sterilize with the jars. I'm Howard Hughes up in here. Nothing can get too clean.

6:02 PM- 90 minutes is up for steam sterilizing. Added an extra 15 to make up for all the times I checked the lid. Only added extra water once. Going to let cool until tomorrow morning and then sterilize the workspace and continue with inoculations.
8/27
9:33 AM- Woke up and about to start cleaning.
12:00P PM - Got done inoculating jars. Did 2 outside SAB and the rest inside. Hoping everything was sterile enough. Now to wait.
9/6
Jars had a nasty odor and dark spots in some of them. Only 1 has any signs of growth. I've moved the 6 smelly jars elsewhere. 3 in one room, 3 in another.
 
9/7
The one jar seems to be slowly moving along. The temp is lower than it should be so I'ma try to get a heater in here.

Ok I see where you sterilized your jars, but did you sterilize your substrate? I have closely read every post on this thread, and maybe I am missing it. Were does it say you pressure cooked the jars with the substrate mixed in it?
Most Likely if you did PC the jars with Substrate, it was the dents. When I prep my jars I flip the lid upside down, drill my holes then flip them the correct direction when I am ready to fill them. This way your holes are aimed up, and any moisture/steam that gets inside the foil will not condensate on the lids and run into your "dry verm" barrier.
I just did 24 jars, 4 B+ with 100% colonization in 9 days. Always make your Substrate on the dry side. To much moisture will mean contamination. We all wish you the best of luck on your next Grow.
Also, I know I shouldn't admit this, but I inoculated with out a SAB and I only flame the needle between jars, not between holes. Im probably the least sterile person on this forum and usually get amazing results. I'll post some pictures soon.
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Stromrider
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Re: Psilocybe Cubensis B+ (First grow) [Re: Sockadin]
#18814195 - 09/08/13 07:33 AM (10 years, 4 months ago) |
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I use a propane torch. Works great. Butane works too
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ShroomPuncher
The FunGuy



Registered: 10/30/12
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Re: Psilocybe Cubensis B+ (First grow) [Re: Sockadin]
#18815143 - 09/08/13 12:58 PM (10 years, 4 months ago) |
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Quote:
Sockadin said:
Quote:
ShroomPuncher said: I'm gonna start my log here. For some reason, it won't let me post in the actual grow log forum. Please, criticize the shit out of me if necessary. I don't wanna ruin my first grow. All suggestions are welcome.
8/26
Woke up, re-watched the BRF videos, re-read some useful info and decided to start.
Even though it's not necessary, I used Lysol wipes to sanitize the part of the kitchen I'd be using. Punched holes in the lid jars (1/2 pint, wide mouth), and doing it wrong, some of them dented in a little which concerned me about getting leaks when steam sterilizing. I'll just wrap extra good with aluminum foil.
Made the substrate. 2 cups verm, 1 cup water. Mixed. Added 1 cup BRF. Mixed. A little chunky but the chunks break easy. Filled 4 jars, wiped moisture / substrate off the 1/2 inch or so off the top of the jar. Everything's not perfectly level. I'm sure this will be fine? 
Ran out of substrate. I'm gonna mix a little bit more and add a couple jars to the mix out of the extra I have. Maybe 6-7 total jars.
Getting back to work. Will update soon while sterilizing. Going to elevate the jars approx. 1/2 inch from the water (using lids and foil), then boil for 90 minutes, checking for steam ever so often.
Jars filled up
 
I ran out of lids and found some old ones but the tops seemed to have mold or rust? The rings themselves though had nothing on them. Still, I'm boiling them then wiping them with alcohol before putting them in the pot to sterilize with the jars. I'm Howard Hughes up in here. Nothing can get too clean.

6:02 PM- 90 minutes is up for steam sterilizing. Added an extra 15 to make up for all the times I checked the lid. Only added extra water once. Going to let cool until tomorrow morning and then sterilize the workspace and continue with inoculations.
8/27
9:33 AM- Woke up and about to start cleaning.
12:00P PM - Got done inoculating jars. Did 2 outside SAB and the rest inside. Hoping everything was sterile enough. Now to wait.
9/6
Jars had a nasty odor and dark spots in some of them. Only 1 has any signs of growth. I've moved the 6 smelly jars elsewhere. 3 in one room, 3 in another.
 
9/7
The one jar seems to be slowly moving along. The temp is lower than it should be so I'ma try to get a heater in here.

Ok I see where you sterilized your jars, but did you sterilize your substrate? I have closely read every post on this thread, and maybe I am missing it. Were does it say you pressure cooked the jars with the substrate mixed in it?
Most Likely if you did PC the jars with Substrate, it was the dents. When I prep my jars I flip the lid upside down, drill my holes then flip them the correct direction when I am ready to fill them. This way your holes are aimed up, and any moisture/steam that gets inside the foil will not condensate on the lids and run into your "dry verm" barrier.
I just did 24 jars, 4 B+ with 100% colonization in 9 days. Always make your Substrate on the dry side. To much moisture will mean contamination. We all wish you the best of luck on your next Grow.
Also, I know I shouldn't admit this, but I inoculated with out a SAB and I only flame the needle between jars, not between holes. Im probably the least sterile person on this forum and usually get amazing results. I'll post some pictures soon.
Good point about drilling holes. And I steam sterilized them in a pot for 90 minutes. One of the jars has really taken off today but it has a orange tinge in it. I'll post pics soon!
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lessismore
Registered: 02/10/13
Posts: 6,268
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18815210 - 09/08/13 01:13 PM (10 years, 4 months ago) |
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Alcohol lamp is a good thing
and glovebox
after glovebox my success went up a lot, before that I got a lot of trich from openair innocs (got lots of spores in my house)
no contams now in any jars, before it was a bit random...
heat needle redhot between each jar (cross sterilize) , no need to wipe it(can cause contam), but I wipe jars with alcohol pads before innoc (if I don't have alufoil over them) , i.e. LC jars with innoc ports
agar is another good thing, syringes can contam easily / be contamed when you buy it (bacteria i.e.) bacteria will be noticeable with an unpleasant odor/almost transparent mycellium in the jars instead of bright white and easy to spot on agar.... although not always easy to isolate away from
after first success I think agar is important (and making sporeprints for future study... place caps on alufoil, put something over them for 24hrs or so - cup or tupperware box i.e.) cloning without agar I didn't have much luck with, contamed with bacteria but agar is easy, then you get contam-free syringes (agar->LC i.e. or agar->grains, or agar->syringe)
when/if you make a glovebox, don't attach gloves to it (still air box) spray the box with alcohol, wear gloves, heat the needle outside the glovebox till redhot, bring it in same with inoculation loops, heat redhot outside the glovebox, bring it in glovebox/stillair box is almost essential IMO, can be hidden under the bed (but having it on a shelf is even better, less spores there than on the ground)
Edited by lessismore (09/08/13 01:21 PM)
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18815215 - 09/08/13 01:14 PM (10 years, 4 months ago) |
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Here's the jar I'm keeping my eye on.

Bottom

It seems to be colonizing still but there's an orange spot on the mycelium. I think you can see it in the pic.
Here's a smelly jar:

All the smelly jars are developing somewhat like this one. The smell and dark spots remain.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: lessismore]
#18815221 - 09/08/13 01:15 PM (10 years, 4 months ago) |
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Quote:
mio said: Alcohol lamp is a good thing
and glovebox
after glovebox my success went up a lot, before that I got a lot of trich from openair innocs (got lots of spores in my house)
no contams now in any jars, before it was a bit random...
heat needle redhot between each jar (cross sterilize) , no need to wipe it(can cause contam), but I wipe jars with alcohol pads before innoc (if I don't have alufoil over them) , i.e. LC jars with innoc ports
agar is another good thing, syringes can contam easily / be contamed when you buy it (bacteria i.e.) bacteria will be noticeable with an unpleasant odor/almost transparent mycellium in the jars instead of bright white
after first success I think agar is important cloning without agar I didn't have much luck with, contamed with bacteria but agar is easy, then you get contam-free syringes (agar->LC i.e. or agar->grains, or agar->syringe)
Good tips. Thanks! The mycelium in my jar doesn't seem pure white. What do you think?
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Sockadin



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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18815237 - 09/08/13 01:20 PM (10 years, 4 months ago) |
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Sounds to me like you got some major problems there, I would really revisit the teks and try and figure out why. This is what they should look like.

Except I prefer the jelly jars instead of standard wide mouth. I always have faster colonization times with Jelly Jars.
Also, I like to mix my BRF and Verm dry then add water. I don't know if this is the right way to do it, but I never have contamination issues.
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lessismore
Registered: 02/10/13
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18815253 - 09/08/13 01:24 PM (10 years, 4 months ago) |
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Most spores start as grey
Be it cubensis spores, trich - and bacteria will look grey too
let it grow out, sniff the jar if it looks 100% colonized, trow it out/PC it if it doesn't look 100% colonized
never sniff jars that aren't 100% colonized
you will be able to tell if there are bacteria/mold in, by sniffing the jars when 100% colonized
moldy/sour/sweet/rotten , unpleasant smell is bad jar good jars smell like fresh mushrooms, are bright white usually after 100% colonization, and smell so good you want to smell them again
bad jars smell a bit bad, almost hurt the nose, you don't feel like smelling it again
bad jars often smell so bad you can smell it outside the jars by being near them, but not always, bacteria has a faint smell to it sometimes (therefore give cakes a good sniff after 100% colonization, sniff again if in doubt, don't birth cakes you are in doubt about)
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: lessismore]
#18815265 - 09/08/13 01:28 PM (10 years, 4 months ago) |
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I mean I've walked through all my steps, double checked, everything. I was pretty clean and took more steps for sterilization than most of the teks. I'm thinking something happened during inoculation.. maybe I was too slow, candle soot, maybe I 'over-sterilized' equipment and messed with it too much in the process.
I'm just glad I'm able to restart tomorrow. I'll only have enough for about 2 jars but all in all, that should be enough for me.
Quote:
mio said: Most spores start as grey
Be it cubensis spores, trich - and bacteria will look grey too
let it grow out, sniff the jar if it looks 100% colonized, trow it out/PC it if it doesn't look 100% colonized
never sniff jars that aren't 100% colonized
you will be able to tell if there are bacteria/mold in, by sniffing the jars when 100% colonized
moldy/sour/sweet/rotten , unpleasant smell is bad jar good jars smell like fresh mushrooms, are bright white usually after 100% colonization, and smell so good you want to smell them again
bad jars smell a bit bad, almost hurt the nose, you don't feel like smelling it again
bad jars often smell so bad you can smell it outside the jars by being near them
Thank you for the advice! Good stuff.
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Sockadin



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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18815305 - 09/08/13 01:41 PM (10 years, 4 months ago) |
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Go buy a pressure Cooker! it will be the best thing you have ever purchased. I know that there are teks that say you can do this without one, but that is crap. By a PC, Dry Mix your sub then add water. And screw the recipe, add water till it looks like you need a little more and stop. If you mix on the dry side you will always have better results.
Most importantly are those inverted holes in your lids, you can always flip them upside down and use them like that.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Sockadin]
#18815324 - 09/08/13 01:47 PM (10 years, 4 months ago) |
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Quote:
Sockadin said: Go buy a pressure Cooker! it will be the best thing you have ever purchased. I know that there are teks that say you can do this without one, but that is crap. By a PC, Dry Mix your sub then add water. And screw the recipe, add water till it looks like you need a little more and stop. If you mix on the dry side you will always have better results.
Most importantly are those inverted holes in your lids, you can always flip them upside down and use them like that.
They are, but I was worried about puncturing the aluminum foil. And honestly, I'm extremely broke and a PC isn't an option. I've seen quite a few people grow with good results and without a pressure cooker. No doubt it's more efficient, but it's not even an option right now, sadly.
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lessismore
Registered: 02/10/13
Posts: 6,268
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18815360 - 09/08/13 01:59 PM (10 years, 4 months ago) |
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Quote:
ShroomPuncher said:
Quote:
Sockadin said: Go buy a pressure Cooker! it will be the best thing you have ever purchased. I know that there are teks that say you can do this without one, but that is crap. By a PC, Dry Mix your sub then add water. And screw the recipe, add water till it looks like you need a little more and stop. If you mix on the dry side you will always have better results.
Most importantly are those inverted holes in your lids, you can always flip them upside down and use them like that.
They are, but I was worried about puncturing the aluminum foil. And honestly, I'm extremely broke and a PC isn't an option. I've seen quite a few people grow with good results and without a pressure cooker. No doubt it's more efficient, but it's not even an option right now, sadly.
pftek is possible without a PC, but you will get more luck with a PC
$77 for a PC here in most stores, and $60 in many stores
won't be 15 psi at that price likely, but 12 psi is better than a pot
you can also wait, remember to buy the largest PC you can get when you get money, it can't be too large (grain jars are quarts size, and you often need 8 of them) and good for pasteurizing too, there it needs to be as large as possible as well (straw/coir) a good 15psi pc is $150+ (presto i.e.) iirc
PC is the most important thing in this hobby
the larger it is the more time you save, PC in the evening, innoc in the morning  would be nice if I could PC 12 pfjars at once, or 8 grain jars at once, much easier than PCing 2-3 times and much easier for straw too, have to pasteurize 2-3 times with the small one I got for enough bulk sub
the seal is bad on cheap PCs too often... breaks in no time, then you need to buy a new seal and if the seal breaks, you just lost all your PCed jars... they will contam, and you won't know the seal broke always
Edited by lessismore (09/08/13 02:05 PM)
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tlien


Registered: 10/07/12
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Re: Psilocybe Cubensis B+ (First grow) [Re: lessismore]
#18815635 - 09/08/13 03:32 PM (10 years, 4 months ago) |
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I'm in Scandinavia, so there is probably, and I'm not an expert, significantly less microbiology in the environment compared to, say, Florida, due to the relatively low temperature and humidity.
However, I made 12 jars, and only one of them became contaminated.
I steamed them for 90 minutes, I used lots of alcohol on my hands and tools, I used a lighter to sterilize the syringe. I showered and put on clean clothes when I worked.
However, I do not have a pressure cooker, flow hood, glove box, or anything like that.
And I used glasses with double foil layers, no lids.
I even put the glasses in a shopping bag and took them with me on an hour long bus trip the two three days later.
And still, only 1/12 was contaminated.
Since you went WAY beyond my approach, it leads me to suspect your syringe contents were contaminated to begin with.
Did you receive the syringe, or a print? Did you use a fresh and sterile, out-of-the-pack syringe and needle?
But I dunno, if you can get a pressure cooker cheaply, might be worth a shot, this is generally a pretty cheap hobby, one pressure cooker shouldn't break the budget, I know a lot of people are enthusiastic about them, it's just that I had no problems in my own subjective experience leading me to doubt they are crucial.
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Sockadin



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Re: Psilocybe Cubensis B+ (First grow) [Re: tlien]
#18815681 - 09/08/13 03:45 PM (10 years, 4 months ago) |
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Quote:
tlien said: I'm in Scandinavia, so there is probably, and I'm not an expert, significantly less microbiology in the environment compared to, say, Florida, due to the relatively low temperature and humidity.
However, I made 12 jars, and only one of them became contaminated.
I steamed them for 90 minutes, I used lots of alcohol on my hands and tools, I used a lighter to sterilize the syringe. I showered and put on clean clothes when I worked.
However, I do not have a pressure cooker, flow hood, glove box, or anything like that.
And I used glasses with double foil layers, no lids.
I even put the glasses in a shopping bag and took them with me on an hour long bus trip the two three days later.
And still, only 1/12 was contaminated.
Since you went WAY beyond my approach, it leads me to suspect your syringe contents were contaminated to begin with.
Did you receive the syringe, or a print? Did you use a fresh and sterile, out-of-the-pack syringe and needle?
But I dunno, if you can get a pressure cooker cheaply, might be worth a shot, this is generally a pretty cheap hobby, one pressure cooker shouldn't break the budget, I know a lot of people are enthusiastic about them, it's just that I had no problems in my own subjective experience leading me to doubt they are crucial.
Jars with out lids? Wow that is awesome. Good Looking oUt.
Here is the thing, I see so many people spend $12-15 per syringe $5-8 on 12 1/2 pint jars. $3-4 Bucks on Perlite $4-5 Bucks on Verm $2-3 On BRF Why not drop another $35 on a PC... Best thing I ever bought.
Imagine spending all that money and loosing all 12 jars. Versus investing another 35 and have a really good chance at fruiting so you can make your own isolate or MS print.
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Kjetterfaen
Strangler

Registered: 07/21/13
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Re: Psilocybe Cubensis B+ (First grow) [Re: tlien]
#18815691 - 09/08/13 03:47 PM (10 years, 4 months ago) |
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It was a syringe directly from a sponsor if I'm not mistaken.
Regarding Scandinavian climate; I was thinking the same thing. I mean, I haven't been doing this for long enough to have consistent results, but thus far I haven't even seen contams in the jars where I openly invited for it. During my first inoculation I knocked up the last jar (after doing the rest with proper precautions) in the kitchen in open air, with the used syringe and water directly from the faucet. No flaming, no precautions, sloppy as hell.. performed exactly the same as the rest of my jars.
And yeah; I can't find mason jars with proper wide shoulders, not do I have a PC, so I am using double foil and steam as well.
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lessismore
Registered: 02/10/13
Posts: 6,268
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18815778 - 09/08/13 04:09 PM (10 years, 4 months ago) |
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Putting drinking glasses with brf in in shopping basket sounds like a bad idea(to the poster mentioning this..)
don't shake pfjars, you disturb the dry verm layer
move them carefully after innoc, and also before innoc (after pc)
I've gotten contams before from tilting my pfjars... trich
prefer to put them on a shelf, they tolerate light fine, it helps during all growth stages
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: lessismore]
#18815820 - 09/08/13 04:23 PM (10 years, 4 months ago) |
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Yup, it was from a sponsor and the needle was out-of-package.
Here's another pic I found on my camera. Don't even remember which jar it is.

And this is the lamp I'll be using for tomorrow.

Better than a candle and now I don't have shaky hands to blame.
And good mention on the tilting. I may have tilted my jars very slightly, but probably not enough to even to disturb the dry verm layer. And I think I went a little thick with my dry verm, too.
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Kjetterfaen
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18815943 - 09/08/13 05:03 PM (10 years, 4 months ago) |
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Denatured alcohol is green over there? Haha it makes it look like something out of a bad horror movie. "Ah, my zombie blood is ready!"
It's pinkish red over here... Anyways, the ghettoburner looks to be all good, and I see you have tested it already =)
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Sockadin



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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18815954 - 09/08/13 05:05 PM (10 years, 4 months ago) |
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IM so lost. Are you innoc another batch? You're problem is in the PC and/or the syringe.
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Kjetterfaen
Strangler

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Re: Psilocybe Cubensis B+ (First grow) [Re: Sockadin] 1
#18815964 - 09/08/13 05:09 PM (10 years, 4 months ago) |
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He is testing the remaining 2-3 ml in the syringe to see if he gets identical results.
I don't think the problem stems from lack of PC as the contamination is happening on the inoculation sites. I think it was introduced by the needle or the spore solution.
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heratogwea



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Re: Psilocybe Cubensis B+ (First grow) [Re: Sockadin]
#18815988 - 09/08/13 05:14 PM (10 years, 4 months ago) |
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Quote:
Sockadin said: Go buy a pressure Cooker! it will be the best thing you have ever purchased. I know that there are teks that say you can do this without one, but that is crap. By a PC, Dry Mix your sub then add water. And screw the recipe, add water till it looks like you need a little more and stop. If you mix on the dry side you will always have better results.
Most importantly are those inverted holes in your lids, you can always flip them upside down and use them like that.
Crap? I have 100% success with steaming so far. Only contams have been due to a bad syringe.
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Sockadin



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Re: Psilocybe Cubensis B+ (First grow) [Re: heratogwea]
#18815999 - 09/08/13 05:20 PM (10 years, 4 months ago) |
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Quote:
heratogwea said:
Quote:
Sockadin said: Go buy a pressure Cooker! it will be the best thing you have ever purchased. I know that there are teks that say you can do this without one, but that is crap. By a PC, Dry Mix your sub then add water. And screw the recipe, add water till it looks like you need a little more and stop. If you mix on the dry side you will always have better results.
Most importantly are those inverted holes in your lids, you can always flip them upside down and use them like that.
Crap? I have 100% success with steaming so far. Only contams have been due to a bad syringe.
Crap! If you are successful, you can print and make your own syringe.
All I'm saying is that if your having trouble with contam's, and your as sterile as this guy maybe it is the method of sterlitity.
We can all wash our hand before we eat. Doesn't mean our hands are clean.
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heratogwea



Registered: 05/05/11
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Re: Psilocybe Cubensis B+ (First grow) [Re: Sockadin]
#18816007 - 09/08/13 05:22 PM (10 years, 4 months ago) |
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Quote:
Sockadin said:
Quote:
heratogwea said:
Quote:
Sockadin said: Go buy a pressure Cooker! it will be the best thing you have ever purchased. I know that there are teks that say you can do this without one, but that is crap. By a PC, Dry Mix your sub then add water. And screw the recipe, add water till it looks like you need a little more and stop. If you mix on the dry side you will always have better results.
Most importantly are those inverted holes in your lids, you can always flip them upside down and use them like that.
Crap? I have 100% success with steaming so far. Only contams have been due to a bad syringe.
Crap! If you are successful, you can print and make your own syringe.
All I'm saying is that if your having trouble with contam's, and your as sterile as this guy maybe it is the method of sterlitity.
We can all wash our hand before we eat. Doesn't mean our hands are clean.
True. I also don't use a sab, flow hood, or anything like that. I'm just quick and extra clean.
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Kjetterfaen
Strangler

Registered: 07/21/13
Posts: 495
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Re: Psilocybe Cubensis B+ (First grow) [Re: Sockadin]
#18816014 - 09/08/13 05:23 PM (10 years, 4 months ago) |
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Quote:
Sockadin said: Crap! If you are successful, you can print and make your own syringe.
And you can't do that with steam sterilization?
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Sockadin



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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18816047 - 09/08/13 05:32 PM (10 years, 4 months ago) |
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I don't use any of that stuff either.
I started making a flow hood out of some plywood and a bathroom exhaust fan today. I will follow up with pic's later. I don't feel like a flow hood is necessary unless your G2G or Agar, but with PF it is so easy and contam free ass long as you get your Water/BRFVERM ratio right.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18816060 - 09/08/13 05:36 PM (10 years, 4 months ago) |
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Quote:
Kjetterfaen said: He is testing the remaining 2-3 ml in the syringe to see if he gets identical results.
I don't think the problem stems from lack of PC as the contamination is happening on the inoculation sites. I think it was introduced by the needle or the spore solution.
^
And I'm using 70% iso in the ghetto lamp.
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Edited by ShroomPuncher (09/08/13 05:39 PM)
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PussyFart
Retired Cultivation Extrodinaire



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Re: Psilocybe Cubensis B+ (First grow) [Re: Sockadin]
#18816072 - 09/08/13 05:39 PM (10 years, 4 months ago) |
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Quote:
Sockadin said: I don't use any of that stuff either.
I started making a flow hood out of some plywood and a bathroom exhaust fan today.
Flow Hoods use blowers, not fans.
And a fart fan is not nearly strong enough...
Get a blower.
Or just build a SAB and call it a day.
--------------------
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Sockadin



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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18816076 - 09/08/13 05:40 PM (10 years, 4 months ago) |
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Quote:
Kjetterfaen said:
Quote:
Sockadin said: Crap! If you are successful, you can print and make your own syringe.
And you can't do that with steam sterilization?
You can but, why risk the cont? Steam Sterilization is one of the most common problems. That is all i'm saying. If you want to increase your chance at success, go spend the money. If you can't afford the cost, don't come on here and bitch when your Jars/Grain Contams.
I got a PC going off in the kitchen right now, it says,"Shoss, Shossss, Shoss, Shoss,"
I guess my point is not that you can't do it without, Just that you shouldn't.
BTW Notahacker, what is the desired CFM for a FlowHood? Im just Effing around in my woodshop and will probably never use the damn thing anyway.. But do you really need like 15-20 CFM for a flow hood?
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jonnymushroom
the man of many teks

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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18816083 - 09/08/13 05:42 PM (10 years, 4 months ago) |
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Nice work and pics . Good luck vibes your why.
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PussyFart
Retired Cultivation Extrodinaire



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Re: Psilocybe Cubensis B+ (First grow) [Re: Sockadin]
#18816090 - 09/08/13 05:43 PM (10 years, 4 months ago) |
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Quote:
Sockadin said: BTW Notahacker, what is the desired CFM for a FlowHood? Im just Effing around in my woodshop and will probably never use the damn thing anyway.. But do you really need like 15-20 CFM for a flow hood?
You need a lot more than that...it all depends on the specs of your filter.
--------------------
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Kjetterfaen
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Re: Psilocybe Cubensis B+ (First grow) [Re: Sockadin]
#18816097 - 09/08/13 05:45 PM (10 years, 4 months ago) |
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Quote:
Sockadin said:
Quote:
Kjetterfaen said:
Quote:
Sockadin said: Crap! If you are successful, you can print and make your own syringe.
And you can't do that with steam sterilization?
You can but, why risk the cont?
Oh I don't disagree that any risk eliminated is good, but I guess my question is; when do steam sterilization even come into it? When do you need the pressure cooking when making prints or syringes?
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Sockadin]
#18816098 - 09/08/13 05:45 PM (10 years, 4 months ago) |
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Quote:
Sockadin said:
Quote:
Kjetterfaen said:
Quote:
Sockadin said: Crap! If you are successful, you can print and make your own syringe.
And you can't do that with steam sterilization?
You can but, why risk the cont? Steam Sterilization is one of the most common problems. That is all i'm saying. If you want to increase your chance at success, go spend the money. If you can't afford the cost, don't come on here and bitch when your Jars/Grain Contams.
I got a PC going off in the kitchen right now, it says,"Shoss, Shossss, Shoss, Shoss,"
I guess my point is not that you can't do it without, Just that you shouldn't.
So no PC = no bitching about contams?
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Kjetterfaen
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18816109 - 09/08/13 05:49 PM (10 years, 4 months ago) |
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I get his point though; don't take shortcuts and then bitch.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18816116 - 09/08/13 05:51 PM (10 years, 4 months ago) |
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Quote:
Kjetterfaen said: I get his point though; don't take shortcuts and then bitch.
If you're able to take shortcuts, I agree. I'm personally doing the best I can with what I have, though. I plan on getting a PC before too long but at the moment, it's not possible.
But I don't think my contams came from steam sterilization or lack of.
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Kjetterfaen
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18816127 - 09/08/13 05:54 PM (10 years, 4 months ago) |
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Quote:
ShroomPuncher said:
Quote:
Kjetterfaen said: I get his point though; don't take shortcuts and then bitch.
If you're able to take shortcuts, I agree. I'm personally doing the best I can with what I have, though. I plan on getting a PC before too long but at the moment, it's not possible.
But I don't think my contams came from steam sterilization or lack of.
I don't think you have been taking shortcuts, and I don't think you have been bitching haha.
If you get the same results with other spore sources as well then it's time to start thinking about a PC though...
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Stromrider
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18816139 - 09/08/13 05:57 PM (10 years, 4 months ago) |
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A pc is never a bad idea in mycology. I say if you got the money get one even if you are just doing cakes
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Stromrider]
#18816187 - 09/08/13 06:11 PM (10 years, 4 months ago) |
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It's something I'm gonna start checking out soon. Definitely if I have bad results again.
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Sockadin



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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher] 1
#18816213 - 09/08/13 06:20 PM (10 years, 4 months ago) |
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I apologize, I really don't think anyone here wishes bad results on people. I have had many Corona's and was just trying to stress the point.
I really hope you do well in the next batch ShroomPuncher. I just think that the risk out weights the benefit of any type of culture and not PC'ing.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Sockadin]
#18816283 - 09/08/13 06:38 PM (10 years, 4 months ago) |
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Quote:
Sockadin said: I apologize, I really don't think anyone here wishes bad results on people. I have had many Corona's and was just trying to stress the point.
I really hope you do well in the next batch ShroomPuncher. I just think that the risk out weights the benefit of any type of culture and not PC'ing.
No, nothing but awesome people on here. 
And I agree with you 100% and I appreciate the advice on the PCs for sure! For all I know, maybe that would resolve my problem. Gonna keep my eyes open as I'll be in town off and on for the week.
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Sockadin



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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18816429 - 09/08/13 07:24 PM (10 years, 4 months ago) |
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Using mine right now.... Cheers!
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Sockadin]
#18818480 - 09/09/13 10:50 AM (10 years, 4 months ago) |
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Prepped 3 jars. 2 for inoculation, 1 as a blank. This time I'm using a rack instead of lids.
 
This way I can elevate my jars and add more water so there's enough steam. I'm gonna sterilize for 90-120 minutes. My lid is somewhat tight but steam still escapes it very subtly. I'm assuming this is fine since some steam has to escape?
Will update tomorrow after inocs
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Kjetterfaen
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18818499 - 09/09/13 10:56 AM (10 years, 4 months ago) |
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Should be great man.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18818539 - 09/09/13 11:14 AM (10 years, 4 months ago) |
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Thanks, bud.
Does anyone have an opinion on this? The orangeish spots are a little more apparent now. I've also noticed some white spots that are darker than others (like in this pic)
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d9c6



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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18818981 - 09/09/13 01:51 PM (10 years, 4 months ago) |
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Quote:
ShroomPuncher said: Thanks, bud.
Does anyone have an opinion on this? The orangeish spots are a little more apparent now. I've also noticed some white spots that are darker than others (like in this pic)

Maybe metabolites? I have no idea.
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Kjetterfaen
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Re: Psilocybe Cubensis B+ (First grow) [Re: d9c6]
#18818989 - 09/09/13 01:53 PM (10 years, 4 months ago) |
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Was that the one that smelled ok?
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18821446 - 09/09/13 11:55 PM (10 years, 4 months ago) |
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It smelled iffy but it smells completely better now.
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FrankHorrigan
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Re: Psilocybe Cubensis B+ (First grow) [Re: d9c6]
#18821483 - 09/10/13 12:09 AM (10 years, 4 months ago) |
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Quote:
ShroomPuncher said:

Looks like you have yeast in that jar. It is contam'd. Sorry 
Make sure to re-sterilize the jar before you discard the contents. Yeast is very prolific.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: FrankHorrigan]
#18822853 - 09/10/13 10:41 AM (10 years, 4 months ago) |
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Quote:
FrankHorrigan said:
Quote:
ShroomPuncher said:

Looks like you have yeast in that jar. It is contam'd. Sorry 
Make sure to re-sterilize the jar before you discard the contents. Yeast is very prolific.

Well I'm about to nocc up two more so I'll see what happens.
Things I'll be doing differently
I steam sterilized for a little bit longer and a little bit hotter. Don't know if that does much. 
I'll be using my ghetto lamp so I can be quicker inside my SAB.
If I barely penetrate the dry verm layer with the needle, is there a less likely chance of contams?
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Kjetterfaen
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18822861 - 09/10/13 10:44 AM (10 years, 4 months ago) |
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Quote:
ShroomPuncher said: If I barely penetrate the dry verm layer with the needle, is there a less likely chance of contams?
I'u'nno... But you want your inoculation well beyond the verm barrier into the substrate anyway, dontcha?
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18822887 - 09/10/13 10:49 AM (10 years, 4 months ago) |
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That's what I'd imagine. I dunno haha. I'm trying to think of anything else I could possibly do to prevent contams this time. But I'm about to do a little research, get some coffee, and call it sterile o'clock and start cleaning.
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Kjetterfaen
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18822902 - 09/10/13 10:52 AM (10 years, 4 months ago) |
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I think you have covered most of the precautions you can take at home as a beginning hobbyist. Relax and hope for the best, and don't worry; you always have another shot at it =)
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heratogwea



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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18823277 - 09/10/13 12:41 PM (10 years, 4 months ago) |
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Quote:
ShroomPuncher said:
Quote:
FrankHorrigan said:
Quote:
ShroomPuncher said:

Looks like you have yeast in that jar. It is contam'd. Sorry 
Make sure to re-sterilize the jar before you discard the contents. Yeast is very prolific.

Well I'm about to nocc up two more so I'll see what happens.
Things I'll be doing differently
I steam sterilized for a little bit longer and a little bit hotter. Don't know if that does much. 
I'll be using my ghetto lamp so I can be quicker inside my SAB.
If I barely penetrate the dry verm layer with the needle, is there a less likely chance of contams?
Why would how far you penetrate have anything to do with contams? If your sterile procedure is good, there will be no contams anywhere in your jar.
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Pestile

Registered: 05/02/13
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Loc: Northern Europe
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Re: Psilocybe Cubensis B+ (First grow) [Re: heratogwea]
#18823294 - 09/10/13 12:44 PM (10 years, 4 months ago) |
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A wet verm layer will be a way for contams to enter the substrate. Inoculate into the substrate.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Pestile]
#18823347 - 09/10/13 12:58 PM (10 years, 4 months ago) |
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Appreciate the advice, guys.
I shot up these 3 jars.

I had some extra spore juice so I went ahead and shot up 3/4 holes on the 'blank'. I feel a lot more confident on these. I used a SAB the whole time and for the first 2, flame sterilized the syringe pretty quickly. I also tried a new room, which actually had 2 doors leading to it. Used lots of alcohol and Lysol air spray.
Just put a heater in my room to try and keep it at a consistent 75 degrees. Fingers crossed.
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Kjetterfaen
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18823402 - 09/10/13 01:14 PM (10 years, 4 months ago) |
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Crossed limbs all over man. I think you should have kept the blank though, would be nice to see how it fared. But I don't really think your problem lies with the substrate so yeah, I get why you went for knocking it up.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18823418 - 09/10/13 01:18 PM (10 years, 4 months ago) |
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Quote:
Kjetterfaen said: Crossed limbs all over man. I think you should have kept the blank though, would be nice to see how it fared. But I don't really think your problem lies with the substrate so yeah, I get why you went for knocking it up.
Yeah, it was between adding a little more spores to the already spore'd up jars or go for another. I should've made an extra blank to begin with lol.
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hidyn
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18823540 - 09/10/13 01:50 PM (10 years, 4 months ago) |
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I'm definitely going to be checking in on this thread to follow these new jars. Wishing you the best of luck! But I'll betcha this batch is going to have very positive results.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: hidyn]
#18823672 - 09/10/13 02:36 PM (10 years, 4 months ago) |
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Thanks, I'm hoping so too!
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18833945 - 09/12/13 07:38 PM (10 years, 4 months ago) |
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No growth from any of the new jars but all the old ones seem to have something going on.

Thought that looked a little different.
I'm just gonna bury these older cakes. If anything else, I heard it'll help the surrounding plant / grass growth. Is there any specifics to planting them? I know I need to wear something different so I don't bring any of the contams in to my new cakes by birthing the old ones... anything else?
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18850970 - 09/16/13 07:58 PM (10 years, 4 months ago) |
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3 of my jars have been removed. They were either discolored, smelly, and one had trich.

I've just noticed this growth in my first group? Is that actually mycelium? I've never seen it so ropey before.
Another of my jars looks the exact same

Here's the one with the most which shit going on but I'm about to toss it. I have no clue if the mycelium is supposed to look like the ones above this one or not.

So, can anyone confirm if either are mycelium.
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Mush4Brains
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18851061 - 09/16/13 08:23 PM (10 years, 4 months ago) |
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The first two are showing nice, strong, ropey rhizomorphic mycelium. They're fine. Toss that last one, open it outdoors and rinse the jar out before bringing it in to wash it with warm soap and water.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Mush4Brains]
#18851071 - 09/16/13 08:25 PM (10 years, 4 months ago) |
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Thanks, I appreciate the feedback!
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Kjetterfaen
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18852314 - 09/17/13 03:34 AM (10 years, 4 months ago) |
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Looks like you got some healthy and normal-looking development in those two first jars... Finally some encouraging developments. How long has it been since inoculation now on those? And, are you seeing development on more then one inoculation site on each jar?
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18853424 - 09/17/13 12:50 PM (10 years, 4 months ago) |
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Quote:
Kjetterfaen said: Looks like you got some healthy and normal-looking development in those two first jars... Finally some encouraging developments. How long has it been since inoculation now on those? And, are you seeing development on more then one inoculation site on each jar?
It's been about 3 weeks and I'm down to 3 of my 7 jars. 4 Have been placed outside for now. 1 developed trich, 1 had the orange spot, and the other 2 had nothing but a few white spots, I believe.

The other

Got some mycelium bat / butterfly stuff goin' on there. 
The other third jar just has the white spot like the other 2 started out with.
The jars with good growth only have growth in 2 of the inoculation points.
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ShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18981725 - 10/15/13 03:10 PM (10 years, 3 months ago) |
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Litte update. All my jars have failed. I've gotten a new syringe and spore prints but I'm waiting on getting new jars. I also am going to change the environment and use a torch to flame sterilize.
I have, however, gotten some aborts/primordial? since there's no more colonization.

That jar is going slightly orange and colonization has stopped. I'm about to bury the cakes outside tomorrow after I get some more aborts, hopefully.
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Gvddd
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#19012349 - 10/22/13 06:56 AM (10 years, 3 months ago) |
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I'm not trying to spam or anything, I'm just trying to help a fellow noob. Maybe you should consider getting a presterilized grow bag like a mad bag. My first grow I used one and I have to admit I wasn't very careful with making sure the area I was inoculating in was100% sterile but even so I got a great growth off it with no cotams.
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Peardu
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#19012358 - 10/22/13 07:03 AM (10 years, 3 months ago) |
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Good luck you will get it.
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loki44
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#19012916 - 10/22/13 10:17 AM (10 years, 3 months ago) |
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Quote:
ShroomPuncher said: Litte update. All my jars have failed. I've gotten a new syringe and spore prints but I'm waiting on getting new jars. I also am going to change the environment and use a torch to flame sterilize.
I have, however, gotten some aborts/primordial? since there's no more colonization.

That jar is going slightly orange and colonization has stopped. I'm about to bury the cakes outside tomorrow after I get some more aborts, hopefully.
Dont throw away that jar! birth it ,scrap away any uncolonized verm, dunk and roll and put it in you SGFC
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loki44
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Re: Psilocybe Cubensis B+ (First grow) [Re: Gvddd]
#19012926 - 10/22/13 10:22 AM (10 years, 3 months ago) |
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Quote:
Gvddd said: I'm not trying to spam or anything, I'm just trying to help a fellow noob. Maybe you should consider getting a presterilized grow bag like a mad bag. My first grow I used one and I have to admit I wasn't very careful with making sure the area I was inoculating in was100% sterile but even so I got a great growth off it with no cotams.
why? to make progress in this hobby we all need to learn proper sterile technique and do the teks from scratch so we learn the kinks and we can "graduate" into more advanced teks
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bushdoc
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Re: Psilocybe Cubensis B+ (First grow) [Re: loki44]
#19019791 - 10/23/13 02:54 PM (10 years, 3 months ago) |
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Bad luck man, I'm just beginning so you have taught me something. We learn more from mistakes.
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