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d9c6



Registered: 07/02/13
Posts: 632
Last seen: 7 years, 4 months
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18818981 - 09/09/13 01:51 PM (10 years, 4 months ago) |
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Quote:
ShroomPuncher said: Thanks, bud.
Does anyone have an opinion on this? The orangeish spots are a little more apparent now. I've also noticed some white spots that are darker than others (like in this pic)

Maybe metabolites? I have no idea.
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Kjetterfaen
Strangler

Registered: 07/21/13
Posts: 495
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Re: Psilocybe Cubensis B+ (First grow) [Re: d9c6]
#18818989 - 09/09/13 01:53 PM (10 years, 4 months ago) |
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Was that the one that smelled ok?
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ShroomPuncher
The FunGuy



Registered: 10/30/12
Posts: 988
Loc: Mother Earth
Last seen: 6 months, 17 days
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18821446 - 09/09/13 11:55 PM (10 years, 4 months ago) |
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It smelled iffy but it smells completely better now.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Psilocybe Cubensis B+ (First grow) [Re: d9c6]
#18821483 - 09/10/13 12:09 AM (10 years, 4 months ago) |
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Quote:
ShroomPuncher said:

Looks like you have yeast in that jar. It is contam'd. Sorry 
Make sure to re-sterilize the jar before you discard the contents. Yeast is very prolific.
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ShroomPuncher
The FunGuy



Registered: 10/30/12
Posts: 988
Loc: Mother Earth
Last seen: 6 months, 17 days
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Re: Psilocybe Cubensis B+ (First grow) [Re: FrankHorrigan]
#18822853 - 09/10/13 10:41 AM (10 years, 4 months ago) |
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Quote:
FrankHorrigan said:
Quote:
ShroomPuncher said:

Looks like you have yeast in that jar. It is contam'd. Sorry 
Make sure to re-sterilize the jar before you discard the contents. Yeast is very prolific.

Well I'm about to nocc up two more so I'll see what happens.
Things I'll be doing differently
I steam sterilized for a little bit longer and a little bit hotter. Don't know if that does much. 
I'll be using my ghetto lamp so I can be quicker inside my SAB.
If I barely penetrate the dry verm layer with the needle, is there a less likely chance of contams?
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Kjetterfaen
Strangler

Registered: 07/21/13
Posts: 495
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18822861 - 09/10/13 10:44 AM (10 years, 4 months ago) |
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Quote:
ShroomPuncher said: If I barely penetrate the dry verm layer with the needle, is there a less likely chance of contams?
I'u'nno... But you want your inoculation well beyond the verm barrier into the substrate anyway, dontcha?
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ShroomPuncher
The FunGuy



Registered: 10/30/12
Posts: 988
Loc: Mother Earth
Last seen: 6 months, 17 days
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18822887 - 09/10/13 10:49 AM (10 years, 4 months ago) |
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That's what I'd imagine. I dunno haha. I'm trying to think of anything else I could possibly do to prevent contams this time. But I'm about to do a little research, get some coffee, and call it sterile o'clock and start cleaning.
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Kjetterfaen
Strangler

Registered: 07/21/13
Posts: 495
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18822902 - 09/10/13 10:52 AM (10 years, 4 months ago) |
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I think you have covered most of the precautions you can take at home as a beginning hobbyist. Relax and hope for the best, and don't worry; you always have another shot at it =)
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heratogwea



Registered: 05/05/11
Posts: 607
Last seen: 3 years, 8 months
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18823277 - 09/10/13 12:41 PM (10 years, 4 months ago) |
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Quote:
ShroomPuncher said:
Quote:
FrankHorrigan said:
Quote:
ShroomPuncher said:

Looks like you have yeast in that jar. It is contam'd. Sorry 
Make sure to re-sterilize the jar before you discard the contents. Yeast is very prolific.

Well I'm about to nocc up two more so I'll see what happens.
Things I'll be doing differently
I steam sterilized for a little bit longer and a little bit hotter. Don't know if that does much. 
I'll be using my ghetto lamp so I can be quicker inside my SAB.
If I barely penetrate the dry verm layer with the needle, is there a less likely chance of contams?
Why would how far you penetrate have anything to do with contams? If your sterile procedure is good, there will be no contams anywhere in your jar.
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Pestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
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Re: Psilocybe Cubensis B+ (First grow) [Re: heratogwea]
#18823294 - 09/10/13 12:44 PM (10 years, 4 months ago) |
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A wet verm layer will be a way for contams to enter the substrate. Inoculate into the substrate.
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   The Corbett Report Open Source Intelligence News
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ShroomPuncher
The FunGuy



Registered: 10/30/12
Posts: 988
Loc: Mother Earth
Last seen: 6 months, 17 days
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Re: Psilocybe Cubensis B+ (First grow) [Re: Pestile]
#18823347 - 09/10/13 12:58 PM (10 years, 4 months ago) |
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Appreciate the advice, guys.
I shot up these 3 jars.

I had some extra spore juice so I went ahead and shot up 3/4 holes on the 'blank'. I feel a lot more confident on these. I used a SAB the whole time and for the first 2, flame sterilized the syringe pretty quickly. I also tried a new room, which actually had 2 doors leading to it. Used lots of alcohol and Lysol air spray.
Just put a heater in my room to try and keep it at a consistent 75 degrees. Fingers crossed.
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Kjetterfaen
Strangler

Registered: 07/21/13
Posts: 495
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18823402 - 09/10/13 01:14 PM (10 years, 4 months ago) |
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Crossed limbs all over man. I think you should have kept the blank though, would be nice to see how it fared. But I don't really think your problem lies with the substrate so yeah, I get why you went for knocking it up.
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ShroomPuncher
The FunGuy



Registered: 10/30/12
Posts: 988
Loc: Mother Earth
Last seen: 6 months, 17 days
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
#18823418 - 09/10/13 01:18 PM (10 years, 4 months ago) |
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Quote:
Kjetterfaen said: Crossed limbs all over man. I think you should have kept the blank though, would be nice to see how it fared. But I don't really think your problem lies with the substrate so yeah, I get why you went for knocking it up.
Yeah, it was between adding a little more spores to the already spore'd up jars or go for another. I should've made an extra blank to begin with lol.
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hidyn
Noob



Registered: 07/14/13
Posts: 268
Loc: Canada
Last seen: 3 months, 18 days
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18823540 - 09/10/13 01:50 PM (10 years, 4 months ago) |
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I'm definitely going to be checking in on this thread to follow these new jars. Wishing you the best of luck! But I'll betcha this batch is going to have very positive results.
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ShroomPuncher
The FunGuy



Registered: 10/30/12
Posts: 988
Loc: Mother Earth
Last seen: 6 months, 17 days
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Re: Psilocybe Cubensis B+ (First grow) [Re: hidyn]
#18823672 - 09/10/13 02:36 PM (10 years, 4 months ago) |
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Thanks, I'm hoping so too!
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ShroomPuncher
The FunGuy



Registered: 10/30/12
Posts: 988
Loc: Mother Earth
Last seen: 6 months, 17 days
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18833945 - 09/12/13 07:38 PM (10 years, 4 months ago) |
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No growth from any of the new jars but all the old ones seem to have something going on.

Thought that looked a little different.
I'm just gonna bury these older cakes. If anything else, I heard it'll help the surrounding plant / grass growth. Is there any specifics to planting them? I know I need to wear something different so I don't bring any of the contams in to my new cakes by birthing the old ones... anything else?
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ShroomPuncher
The FunGuy



Registered: 10/30/12
Posts: 988
Loc: Mother Earth
Last seen: 6 months, 17 days
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18850970 - 09/16/13 07:58 PM (10 years, 4 months ago) |
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3 of my jars have been removed. They were either discolored, smelly, and one had trich.

I've just noticed this growth in my first group? Is that actually mycelium? I've never seen it so ropey before.
Another of my jars looks the exact same

Here's the one with the most which shit going on but I'm about to toss it. I have no clue if the mycelium is supposed to look like the ones above this one or not.

So, can anyone confirm if either are mycelium.
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Mush4Brains
LOOL HACKED!!!

Registered: 07/31/13
Posts: 4,419
Last seen: 9 years, 2 months
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18851061 - 09/16/13 08:23 PM (10 years, 4 months ago) |
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The first two are showing nice, strong, ropey rhizomorphic mycelium. They're fine. Toss that last one, open it outdoors and rinse the jar out before bringing it in to wash it with warm soap and water.
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ShroomPuncher
The FunGuy



Registered: 10/30/12
Posts: 988
Loc: Mother Earth
Last seen: 6 months, 17 days
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Re: Psilocybe Cubensis B+ (First grow) [Re: Mush4Brains]
#18851071 - 09/16/13 08:25 PM (10 years, 4 months ago) |
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Thanks, I appreciate the feedback!
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Kjetterfaen
Strangler

Registered: 07/21/13
Posts: 495
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
#18852314 - 09/17/13 03:34 AM (10 years, 4 months ago) |
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Looks like you got some healthy and normal-looking development in those two first jars... Finally some encouraging developments. How long has it been since inoculation now on those? And, are you seeing development on more then one inoculation site on each jar?
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