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OfflineShroomPuncher
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Psilocybe Cubensis B+ (First grow)
    #18759563 - 08/26/13 01:09 PM (10 years, 5 months ago)

I'm gonna start my log here. For some reason, it won't let me post in the actual grow log forum. Please, criticize the shit out of me if necessary. I don't wanna ruin my first grow. All suggestions are welcome.


8/26

Woke up, re-watched the BRF videos, re-read some useful info and decided to start.

Even though it's not necessary, I used Lysol wipes to sanitize the part of the kitchen I'd be using. Punched holes in the lid jars (1/2 pint, wide mouth), and doing it wrong, some of them dented in a little which concerned me about getting leaks when steam sterilizing. I'll just wrap extra good with aluminum foil.

Made the substrate. 2 cups verm, 1 cup water. Mixed. Added 1 cup BRF. Mixed. A little chunky but the chunks break easy. Filled 4 jars, wiped moisture / substrate off the 1/2 inch or so off the top of the jar. Everything's not perfectly level. I'm sure this will be fine? :shrug:

Ran out of substrate. I'm gonna mix a little bit more and add a couple jars to the mix out of the extra I have. Maybe 6-7 total jars.


Getting back to work. Will update soon while sterilizing. Going to elevate the jars approx. 1/2 inch from the water (using lids and foil), then boil for 90 minutes, checking for steam ever so often.



Jars filled up



I ran out of lids and found some old ones but the tops seemed to have mold or rust? The rings themselves though had nothing on them. Still, I'm boiling them then wiping them with alcohol before putting them in the pot to sterilize with the jars. I'm Howard Hughes up in here. Nothing can get too clean.




6:02 PM- 90 minutes is up for steam sterilizing. Added an extra 15 to make up for all the times I checked the lid. Only added extra water once. Going to let cool until tomorrow morning and then sterilize the workspace and continue with inoculations.



8/27

9:33 AM- Woke up and about to start cleaning.


12:00P PM - Got done inoculating jars. Did 2 outside SAB and the rest inside. Hoping everything was sterile enough. Now to wait.

9/6

Jars had a nasty odor and dark spots in some of them. Only 1 has any signs of growth. I've moved the 6 smelly jars elsewhere. 3 in one room, 3 in another. 



9/7

The one jar seems to be slowly moving along. The temp is lower than it should be so I'ma try to get a heater in here.



9/8

The jar I have the most faith in now has a orange-ish spot, but still seems to be developing well and pretty fast.




Here's a pic of one of the smelly jars. They're all still separated from ol' faithful but I still don't have my hopes high.



All the other smelly ones are coming along like this one.

9/9

I'm sterilizing more jars. The other jars seem to be the same. Not much growth of whatever is growing in there.




Hopefully the 2 (possibly 3) I shoot up tomorrow will have better results. If not, I'm pretty sure it's the syringe.


Edited by ShroomPuncher (09/09/13 10:52 AM)


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Offlinetlien
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
    #18759589 - 08/26/13 01:20 PM (10 years, 5 months ago)

Sounds good, bro.

Keeping things clean is always a good idea. You never know where mold spores can sneak their way into your jars from.

And I wouldn't worry about your substrate filter layer not being level, mushrooms don't care too much heh.

Yeah, everything sounds in order here.


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InvisibleKjetterfaen
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
    #18759647 - 08/26/13 01:42 PM (10 years, 5 months ago)

:popcorn:


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OfflineShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Kjetterfaen]
    #18759790 - 08/26/13 02:27 PM (10 years, 5 months ago)

Boiling now. I appreciate the feedback! My 1 concern is that they may get waterlogged. I covered a few of them (7 total) with extra foil and made sure the foil is on tight. I used a 2:1:1 ratio of verm/water/BRF. Think I'll be good? Probably just paranoid.


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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher] * 1
    #18759819 - 08/26/13 02:35 PM (10 years, 5 months ago)

All the luck in the world to ya! :threadmonitor:


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Offlinetlien
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
    #18759856 - 08/26/13 02:44 PM (10 years, 5 months ago)

That is the correct ratio, yeah!


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OfflineDevilFromRs
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Re: Psilocybe Cubensis B+ (First grow) [Re: tlien]
    #18759969 - 08/26/13 03:11 PM (10 years, 5 months ago)

Diggin the purple oven light. Good start, you can never be too careful going about a sanitized working area for your jars/grow. I also am starting a b+ strain grow via pf tek so I am interested in watching your grow.


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OfflineShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: DevilFromRs]
    #18760014 - 08/26/13 03:21 PM (10 years, 5 months ago)

Quote:

DevilFromRs said:
Diggin the purple oven light. Good start, you can never be too careful going about a sanitized working area for your jars/grow. I also am starting a b+ strain grow via pf tek so I am interested in watching your grow.





That's just how it turned out in the camera haha. But from what I've read, B+ colonizes rather quickly and is more contam resistant... or something else that indicated that it was pretty noob-friendly. Hopefully you can learn from my mistakes.... Or maybe I'll have a clean grow.  :awesomenod:


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OfflineShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
    #18760233 - 08/26/13 04:01 PM (10 years, 5 months ago)

Steam sterilized for 90 minutes. Gonna go ahead and tack on another 15 minutes to make up for all the probably unnecessary times I've lifted the lid. That, and I just want to be safe.


I'm gonna wake up early tomorrow and start sterilizing my workspace and the area around it (Lyson air spray, wiping counters with 70% iso alcohol, mopping, etc.). I have a still air box but it's not comfortable and I want to be able to see what I'm doing for my first inoculations so I can get an idea of the right amount and whatnot. I'll shut all the windows, keep animals in separate rooms, and be wearing a dust or paint mask, tyvec sleeves, and nitrile gloves, which will all be sterilized.


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OfflineKnownPown
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
    #18762190 - 08/27/13 12:39 AM (10 years, 5 months ago)

My first grow was 8 B+ jars and I was tripping on dry shrooms on the 1 month birthday of when I inoculated the jars :laugh:


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OfflineShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: KnownPown]
    #18762770 - 08/27/13 07:31 AM (10 years, 5 months ago)

Quote:

KnownPown said:
My first grow was 8 B+ jars and I was tripping on dry shrooms on the 1 month birthday of when I inoculated the jars :laugh:






Nice! How much did you produce on your first grow?


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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
    #18763159 - 08/27/13 10:05 AM (10 years, 5 months ago)

Inoculation is done. I'm a little disappointed. I lysol'ed the house after vacuuming, sweeping, then mopping the kitchen I'm working in. I then wiped all the counters and most everything else with alcohol.

I started the first two jars without an air box. Noticing that the flame on the lighter was flickering more than I liked, I decided to use the air box, which I cleaned beforehand with bleach Lysol and disinfected a little with alcohol.

My problems are that 1, my lighters went out so I lit a candle and the candle made my needle tip black, which I know some residue from that got into a couple of the jars (Which I wiped BEFORE and AFTER inoculation with alcohol).


Other than that, my hands were a little shaky, I probably didn't get one jar (or hole for that matter) perfect with 1 CC (or 1/4 per hole, although using 7 jars, I have a little under 3 CC remaining in a 10 CC syringe.


Also, when I was heating the needle, whether with a lighter or candle, it kind of spitting out spore solution? I'm assuming this is normal since liquid and fire have that reaction? Also, I flame sterilized the needle for a few seconds in each part until it was red (except right against the syringe since I wanted to avoid burning the syringe plastic itself), but the entire needle was never all at once red hot, just the parts the lighter touched until I moved it along to a different spot.


So, basically my concerns:

-Air wasn't 100% still so I used my ghetto SAB after 2 jars
-Candle made needle black on a couple of occasions.
-The entire needle was at not point completely red hot. Rather, sections were red hot at a time but I feel like it would've killed any contams that would've landed on it?


Thoughts? :shrug:


Also, other than possibly adding more heat, does light speed up colonization at all or is light not a factor?


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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
    #18768551 - 08/28/13 01:51 PM (10 years, 5 months ago)

My first grow was 8 half pint B+ Jars and I just recently threw them out after they grew some cobweb mold after 2nd flush. I harvested about 1.5-2 oz dry total from all flushes off those 8 cakes.

Currently fruiting out the rest of my 8 EQ and 8 GT cakes, GT have given me a great even pinset first flush, B+ gave me a pretty good yield and EQ had few but massive fruits, still averaged 3g dry or 30g wet per cake per flush.

Looks like you did things like I did pretty much, I just inoculated in my ghetto SAB in my closet and used alcohol and a lighter for my first time. Lighter seemed to work fine. I later just put osme alcohol in a small dish so my needle didnt get all nasty.

Goodluck man!


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OfflineShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: KnownPown]
    #18768656 - 08/28/13 02:25 PM (10 years, 5 months ago)

Quote:

KnownPown said:
My first grow was 8 half pint B+ Jars and I just recently threw them out after they grew some cobweb mold after 2nd flush. I harvested about 1.5-2 oz dry total from all flushes off those 8 cakes.

Currently fruiting out the rest of my 8 EQ and 8 GT cakes, GT have given me a great even pinset first flush, B+ gave me a pretty good yield and EQ had few but massive fruits, still averaged 3g dry or 30g wet per cake per flush.

Looks like you did things like I did pretty much, I just inoculated in my ghetto SAB in my closet and used alcohol and a lighter for my first time. Lighter seemed to work fine. I later just put osme alcohol in a small dish so my needle didnt get all nasty.

Goodluck man!





Nice, I wanna give Golden Teachers a try next. Just the name alone is worth the grow. Good to see you had a good grow. Hope I do as well. :cool:


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OfflineShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
    #18781412 - 08/31/13 01:43 PM (10 years, 4 months ago)

Update: 4 days- no signs of colonization or contamination. Temps have probably been about 72 (least) to 79 (most). Hoping for something soon.  :hissyfit:


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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
    #18781504 - 08/31/13 02:18 PM (10 years, 4 months ago)

You're making me mad, OP. Did you remember to aim the syringes towards the edge of the glass? That way, you will see the growth the earliest.

Goddamn, I'm so mad right now. What did your syringes look like? Did you see tiny blobs in it?

SO MAD!


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OfflineStromriderM
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Re: Psilocybe Cubensis B+ (First grow) [Re: tlien]
    #18781519 - 08/31/13 02:24 PM (10 years, 4 months ago)

5 days is the earliest I ever see growth. Sometimes it takes 8 to 10


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OfflineShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: Stromrider]
    #18781723 - 08/31/13 03:34 PM (10 years, 4 months ago)

Quote:

tlien said:
You're making me mad, OP. Did you remember to aim the syringes towards the edge of the glass? That way, you will see the growth the earliest.

Goddamn, I'm so mad right now. What did your syringes look like? Did you see tiny blobs in it?

SO MAD!





:uhoh: Yup, aimed towards the edges and watched it drip down. Syringes were pretty damn dark but from a reputable vendor. And there were clumps but I shook it up pretty well.




Quote:

psillyshroomer said:
5 days is the earliest I ever see growth. Sometimes it takes 8 to 10





That seems like good time. I'll keep that in mind. I have a feeling mine will take a little longer though.


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Re: Psilocybe Cubensis B+ (First grow) [Re: ShroomPuncher]
    #18781953 - 08/31/13 04:33 PM (10 years, 4 months ago)

Dark is good, my man. That means there are numerous spores in there.

I'm only speaking from experience of TWO syringes, which I made myself, one had no visible spores, 3/3 jars survives, with regular growth, one was filled with dark blotches, 6/6 survived, rapid growth.

I hope your jars colonize well :laugh:


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OfflineShroomPuncher
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Re: Psilocybe Cubensis B+ (First grow) [Re: tlien]
    #18782028 - 08/31/13 04:54 PM (10 years, 4 months ago)

Quote:

tlien said:
Dark is good, my man. That means there are numerous spores in there.

I'm only speaking from experience of TWO syringes, which I made myself, one had no visible spores, 3/3 jars survives, with regular growth, one was filled with dark blotches, 6/6 survived, rapid growth.

I hope your jars colonize well :laugh:





Thanks for the reassurance! I'm hoping they come along some time soon.


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