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brownbanana
Stranger
Registered: 04/04/13
Posts: 26
Last seen: 10 years, 5 months
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Been running into some problems lately
#18758523 - 08/26/13 04:51 AM (10 years, 5 months ago) |
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So, my cat used to use jars of birdseed, but recently switched to 2.5 quart spawn bags. The jars used to be fairly successful, but since switching to spawn bags, there's been a lot more problems.
They always seem to colonize fine, but tend to smell a bit funky when opened.
Could I be over stuffing my pressure cooker? Or maybe it's because I haven't been using self healing injection ports like I did with the jars?
Also, is there a higher risk of contamination for the elementary coir tek than for proper pasteurization? I recently had a few tubs fail on me last minute. Just wondering because RR said that proper tubs shouldn't contaminate if the spawn is good.
Just stuck in a rut, thought I'd get from input from you guys.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 13 hours, 11 minutes
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Re: Been running into some problems lately [Re: brownbanana]
#18758595 - 08/26/13 05:56 AM (10 years, 5 months ago) |
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Larger substrates require longer periods of pressure cooking to sterilize since it takes longer for the heat to reach the center. How long did you PC them?
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BloodKil
Mangler av era mödrar slida


Registered: 03/16/13
Posts: 920
Last seen: 1 month, 15 days
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Re: Been running into some problems lately [Re: Kizzle]
#18758609 - 08/26/13 06:07 AM (10 years, 5 months ago) |
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Quote:
Kizzle said: Larger substrates require longer periods of pressure cooking to sterilize since it takes longer for the heat to reach the center. How long did you PC them?
I don't know why people even respond to idiots that start their post with "my (insert random person place or thing) is having issues growing srooms"
OP if your fucking cat is having issues growing shrooms, then have him come make an account. If this however is your ingenious attempt at disguising yourself from a law enforcement officers, then it's not going to make a damn bit of good dumbass. It's just annoying as fuck to have to read about people's animals all day. If you having issues, either start reading, or ask a question as the human being that is having problems.
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 19 hours, 23 minutes
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Re: Been running into some problems lately [Re: BloodKil]
#18758622 - 08/26/13 06:22 AM (10 years, 5 months ago) |
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Not sure Probably yes
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Pestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
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Re: Been running into some problems lately [Re: Stromrider]
#18758627 - 08/26/13 06:26 AM (10 years, 5 months ago) |
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Quote:
psillyshroomer said: Not sure Probably yes
Shabam!
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   The Corbett Report Open Source Intelligence News
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 19 hours, 23 minutes
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Re: Been running into some problems lately [Re: Pestile]
#18758639 - 08/26/13 06:35 AM (10 years, 5 months ago) |
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Quote:
Pestilence said:
Quote:
psillyshroomer said: Not sure Probably yes
Shabam!
Hey you make a post like that then that is all you get
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brownbanana
Stranger
Registered: 04/04/13
Posts: 26
Last seen: 10 years, 5 months
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Re: Been running into some problems lately [Re: Stromrider]
#18759755 - 08/26/13 02:16 PM (10 years, 5 months ago) |
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You guys don't have to be douches. Most of the threads on here are definitely a lot more useless.
I was just trying to figure out what's at fault for my latest problems, and a lot of the info here conflicts, and I'm having trouble finding new info.
Used to never get any contaminated jars, but ever since switching to bags almost 75% have some kind of funk.
Mostly I was hoping someone could pass along some info on infected bags with no visible contamination, because my bags almost never actually show anything wrong, but often smell bad when opened. Could this be some kind of bacterial infection?
I pc for 2 hours at 15 psi.
I recently was stuffing my pc to the very top with bags anywhere from 2.5-5 quarts, but I think I'm gunna cut it down, and use a lot less grains, and rinse them a bit better. From now on I'll also inject through silicone in a glove box, because I think the main cause of problems is that the duct tape was doing a crappy job at keeping the injection hole sealed.
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krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
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Re: Been running into some problems lately [Re: brownbanana]
#18759816 - 08/26/13 02:34 PM (10 years, 5 months ago) |
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I used to have a lot of problems once switching from jars to spawn bags. My problems were bacterial contamination which may have been linked to partial sterilization as well as doing G2G transfers to inoculate my bags. You have to keep in mind that when moving from jars to bags you're essentially putting all your grains in one basket, no pun intended. What this means is *all* of your variables need to be more dialed in. If you colonize a bag via G2G from a jar you better be that much more aware of how clean your original jar is as well as how thoroughly sterilized your myco bag is.
One big factor I noticed is moisture content. Often times as the mycilium will consolidate a bag it creates metabolites which draw the water out of the grains causing the bottom to become over saturated. Once this happens and they sit for too long you are opening up a window for bacterial contamination. My advice is if you notice this happening at all you should err on the side of caution and make your grains less hydrated rather than more, this has made a big difference for me. Also if you are using a grain bag to spawn to bulk I would strongly advise until you have the process down to simply create a self healing injection port on the bag after PCing and simply knock it up like a jar. The best way I've found to do this is to PC the bag, wait for it to cool, and then put some masking tap on it (being slightly worm still will actually help this adhere better), and then use the standard silicone to create an injection port on the patch of masking tape. You don't want to put the silicone directly onto the bag because it does not stick well and has a tendency to fall off. Once you get that process down then I would start looking into G2G transferring, using agar, etc to create bags and the like.
How are you currently inoculating your bags btw? You mentioned you're not using a self healing injection port, are you just opening them up and squirting the spores in there? Even if you're doing that in a GB if the bag/grain is a cooler temperature than ambient it will create airflow sucking spores inwards due to the mass, the liquids inside, the pressure differential from opening the bag (jars being glass/a standard shape this doesn't happen) etc, as well as the size it takes up within the GB (unless you for some reason have a rather large GB). If the top of your bag is vacuumed shut due to the PCing for instance and then you cut it open and actually have to pull it apart to inoculate it what you're doing is pulling outside air in it. If you insist on inoculating it this way shake the bag with the grain in it before opening to force air in through the GE patch.
edit: That last point is also a likely reason for a bacterial contamination, you should always try to maximize the air within your bag before inoculation (by sucking it in through the GE patch). If there is not much air in there initially the mushrooms will essentially suffocate and this too opens up a pathway for bacterial contamination. Another thing I've found useful, which we don't get much of an ability to do with jars, is that after your myc is somewhat colonize you'll notice the bag itself begin to fill up with CO2. It can actually be helpful to push this CO2 out, or again the mycs growth can slow down, and you'll quickly notice (it should be within 24hr) the further creation of gas. What this does is essentially helping the mycilium exchange gas, this is in part what shaking our jars does as well as breaking up/spreading the mycilium, but with bags you have the luxury of control over this variable without having to actually break the colony up which can really help. I typically will do this once or twice before spawning after the bag is fully colonized and I've noticed that the bag will become *much* whiter and healthy looking by doing this once or twice for a couple days in a row before spawning, you're essentially just helping it breath.
Edited by krypto2000 (08/26/13 02:47 PM)
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HypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
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Re: Been running into some problems lately [Re: BloodKil]
#18759846 - 08/26/13 02:41 PM (10 years, 5 months ago) |
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Quote:
BloodKil said:
Quote:
Kizzle said: Larger substrates require longer periods of pressure cooking to sterilize since it takes longer for the heat to reach the center. How long did you PC them?
I don't know why people even respond to idiots that start their post with....
Bloodkil: You really just went overboard. Unfortunately, ALL of us have been new at this hobby at one time. ALL of us have asked or done dumb things (Myself especially).
Being so bold as to assume someone is an idiot makes you a part of another problem that affects all UTUSF. Get off your horse, and try to rationalize things like us peasants for just a short moment.
brownbanana:
As kizzle said, It may not be enough time. A fully stocked and packed PC will take longer to get core temps, so well over 2 hours may be needed for a packed house. Your best approach as you indicated would be to run a few smaller cooking cycles. Verify that you are hitting the 15 PSI, and that your bags are sealed AND have some sort of filtered gas exchange. The smell you indicated more than likely bacteria that either was introduced via inoculation OR from surviving the Pressure cook.
I would also take a look at your inoculation method. Is it possible that you have a contamination within your syringe or print?
Good luck.
P.s. Posting for/as a 3rd party would not save you IF you were under investigation. Apparently some users of this forum are totally unforgiving of this infraction. Keep your head down, and you will do fine here.
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