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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Can you resterilize a grain jar?
#18757279 - 08/25/13 09:40 PM (10 years, 5 months ago) |
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I did a batch of quart jars with brown rice, one lid somehow got loose and open to the open air for a few seconds. Is it possible to re-pc it?
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Re: Can you resterilize a grain jar? [Re: invitro]
#18757359 - 08/25/13 09:55 PM (10 years, 5 months ago) |
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I'm assuming you're talking about BRF for PFtek? If so quart jars will not work, you want the short widemouth 1/2 pints. Even the taller regular mouth 1/2 pints have problems with slow growth and sometimes even completely stall out in comparison.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Can you resterilize a grain jar? [Re: mushmagic]
#18757681 - 08/25/13 11:10 PM (10 years, 5 months ago) |
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whole grain brown rice, in a quart jar.
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San
Novice


Registered: 10/30/11
Posts: 646
Last seen: 9 years, 5 months
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Re: Can you resterilize a grain jar? [Re: invitro]
#18757753 - 08/25/13 11:22 PM (10 years, 5 months ago) |
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uhh sounds weird.
If you've got extra inoculate then try it. Brown rice is dirt cheap though so you might just want to toss it. Why was it opened?
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Actually not everyone was a noob. Being a noob is a very new phenomenon. Many people, the great majority in fact, were simply "beginners", "novices" or "new to mushroom growing". Being a "noob" is reserved, and in fact created specifically for and by, the newer, much more lame generations coming about. -Shpongle1
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Rockhound
The Rockweiler



Registered: 01/19/13
Posts: 664
Loc: hell creek anticline
Last seen: 8 years, 7 months
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Re: Can you resterilize a grain jar? [Re: invitro]
#18757773 - 08/25/13 11:28 PM (10 years, 5 months ago) |
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It can't hurt to try.Have you had success using whole brown rice before? I resterilized 3 wbs jar once successfully,but it may have been a fluke.The main variables I would consider to be moisture content and nutritional loss.
-------------------- Rocks speak to me, and tell me this: The Hell Creek formation is a gigantic slab of rocks that covers several western states. It contains an account of the dinosaurs' demise. In the late Cretaceous period, the first Cannabis species appear, and soon after, all the non-bird dinosaurs disappeared. Obviously, marihuana killed the dinosaurs. That giant meteor that smacked the yucatan peninsula right afterwards, coincidence.
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San
Novice


Registered: 10/30/11
Posts: 646
Last seen: 9 years, 5 months
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Re: Can you resterilize a grain jar? [Re: Rockhound]
#18757984 - 08/26/13 12:14 AM (10 years, 5 months ago) |
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Yeah, repeatedly working the grains like that would eventually turn them into an unusable mush. Especially brown rice.
If it doesn't work then it'll probably be for skipping the soak.
Soak -> PC (While mold spores are germinated and vulnerable) -> Exposure -> Re-pressure cook (With ungerminated spores) -> inoculate -> contam
The ungerminated mold spores might resist the second pressure cooking. I'm going to bet that it will work out okay though, despite the unconventional methods.
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Actually not everyone was a noob. Being a noob is a very new phenomenon. Many people, the great majority in fact, were simply "beginners", "novices" or "new to mushroom growing". Being a "noob" is reserved, and in fact created specifically for and by, the newer, much more lame generations coming about. -Shpongle1
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Can you resterilize a grain jar? [Re: San]
#18758035 - 08/26/13 12:32 AM (10 years, 5 months ago) |
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Quote:
San said: Soak -> PC (While mold spores are germinated and vulnerable) -> Exposure -> Re-pressure cook (With ungerminated spores) -> inoculate -> contam
The ungerminated mold spores might resist the second pressure cooking.
Such bullshit 
Mold spores die easily, ungerminated, at temps as low as 140F.
The long soak is for bacterial endospores but I don't believe those are a problem either, not after a 90 minute PC and proper moisture content.
You sound like you've never done any trials with these things you speak of.
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San
Novice


Registered: 10/30/11
Posts: 646
Last seen: 9 years, 5 months
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Re: Can you resterilize a grain jar? [Re: FrankHorrigan]
#18758124 - 08/26/13 01:00 AM (10 years, 5 months ago) |
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Quote:
FrankHorrigan said:
Quote:
San said: Soak -> PC (While mold spores are germinated and vulnerable) -> Exposure -> Re-pressure cook (With ungerminated spores) -> inoculate -> contam
The ungerminated mold spores might resist the second pressure cooking.
Such bullshit 
Ehh, mold... Bacteria... It's all the same to me.
I'm going to bet that it will work out okay though, despite the unconventional methods.
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Actually not everyone was a noob. Being a noob is a very new phenomenon. Many people, the great majority in fact, were simply "beginners", "novices" or "new to mushroom growing". Being a "noob" is reserved, and in fact created specifically for and by, the newer, much more lame generations coming about. -Shpongle1
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Can you resterilize a grain jar? [Re: San]
#18758141 - 08/26/13 01:07 AM (10 years, 5 months ago) |
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Quote:
San said: The ungerminated mold spores won't ever resist the second a proper pressure cooking cycle.

If you get mold in your jars after a 90-120 min PC @ 15psi, your sterile procedure needs work or your culture is contaminated.
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San
Novice


Registered: 10/30/11
Posts: 646
Last seen: 9 years, 5 months
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Re: Can you resterilize a grain jar? [Re: FrankHorrigan]
#18758162 - 08/26/13 01:11 AM (10 years, 5 months ago) |
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You okay there, Frank?
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Actually not everyone was a noob. Being a noob is a very new phenomenon. Many people, the great majority in fact, were simply "beginners", "novices" or "new to mushroom growing". Being a "noob" is reserved, and in fact created specifically for and by, the newer, much more lame generations coming about. -Shpongle1
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Can you resterilize a grain jar? [Re: San]
#18758165 - 08/26/13 01:12 AM (10 years, 5 months ago) |
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Is there something wrong with providing correct information in place of bad information?
If so, I've been doing this wrong for a long time
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San
Novice


Registered: 10/30/11
Posts: 646
Last seen: 9 years, 5 months
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Re: Can you resterilize a grain jar? [Re: FrankHorrigan]
#18758180 - 08/26/13 01:15 AM (10 years, 5 months ago) |
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I dunno, man. I was told that soaking before pressure cooking is vital, and skipping the soak can lead to contamination.
I wanted my post to reflect that there's more to pressure cooking than just what goes on inside the pot, and that doing things in the right order is important.
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Actually not everyone was a noob. Being a noob is a very new phenomenon. Many people, the great majority in fact, were simply "beginners", "novices" or "new to mushroom growing". Being a "noob" is reserved, and in fact created specifically for and by, the newer, much more lame generations coming about. -Shpongle1
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Can you resterilize a grain jar? [Re: San]
#18758188 - 08/26/13 01:19 AM (10 years, 5 months ago) |
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The argument for the soaking is that bacterial endospores will get you if you don't germinate them..I have left jars uninoculated, PC'd, for months and have never found this to be true.
I soak my grains for hydration. 6 hours, 12 hours, 24 hours...all the same results.
I'm not the only one to question the viability of bacterial endospores after a PC. There has been a lot of discussion in the past.
This of course is all about bacteria.
Mold spores die easily.
You should distinguish between bacteria and mold
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Can you resterilize a grain jar? [Re: FrankHorrigan]
#18758950 - 08/26/13 09:22 AM (10 years, 5 months ago) |
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I have had jars of pced rye get trich after 2-4 weeks, unopened, uninnoculated. The argument is that sometimes spores get into the crease of the grain where they get insulated from the heat of the pc to some degree. Prob. only happens when grains sit around unsold for too long?
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Nakor420
Fun Guy



Registered: 05/02/13
Posts: 1,616
Loc: The Spirit World
Last seen: 9 years, 11 months
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Re: Can you resterilize a grain jar? [Re: San]
#18758975 - 08/26/13 09:32 AM (10 years, 5 months ago) |
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Quote:
San said:
Ehh, mold... Bacteria... It's all the same to me.
mold....and bacteria....the same.....
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Rockhound
The Rockweiler



Registered: 01/19/13
Posts: 664
Loc: hell creek anticline
Last seen: 8 years, 7 months
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Re: Can you resterilize a grain jar? [Re: FrankHorrigan]
#18759240 - 08/26/13 11:04 AM (10 years, 5 months ago) |
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Frank,I had this friend in high school who ask if you thought his outrageously stupid ideas would work,then get mad at you for pointing out its flaws. I thought he would never live to see 20.Apparently though,he sired a great many just like him.
-------------------- Rocks speak to me, and tell me this: The Hell Creek formation is a gigantic slab of rocks that covers several western states. It contains an account of the dinosaurs' demise. In the late Cretaceous period, the first Cannabis species appear, and soon after, all the non-bird dinosaurs disappeared. Obviously, marihuana killed the dinosaurs. That giant meteor that smacked the yucatan peninsula right afterwards, coincidence.
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