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Offlinekrypto2000
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My mono is almost fully colonized and I just spotted an orange metabolite pool, what do?
    #18756717 - 08/25/13 07:36 PM (10 years, 5 months ago)

I'm used to yellow metabolites which is often followed by a trich infection, however this is almost pure orange, though with a slight brown tinge to it. I'm assuming this either means it's having trouble breaking down something or a bacterial infection, or perhaps it's simply stressed. I did just move it to a room with more light a few hours ago and cannot swear it wasn't there when I moved it, but I certainly did not see it until now.

So my questions are:

1.) Does anyone know what an orange metabolite might specifically indicate?

2.) What should I do in the mean time, leave it in the light or move it back to where it was?

3.) After birth should I clean the pool up with a paper towel or let it evaporate on its own?

4.) Is there anything else I should look out for or do in the mean time that I may not be thinking about?

edit: It looks almost exactly like the picture in RR's post here.


Edited by krypto2000 (08/25/13 07:40 PM)


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Offlineblojo02184
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Re: My mono is almost fully colonized and I just spotted an orange metabolite pool, what do? [Re: krypto2000]
    #18756793 - 08/25/13 07:55 PM (10 years, 5 months ago)

1. Nope.
2. Id say leave it. light change may have caused the stress.
3. Check this out. http://www.shroomery.org/forums/showflat.php/Number/18738488/page/1/fpart/all
4. Remain calm.

Good luck man


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Offlinekrypto2000
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Re: My mono is almost fully colonized and I just spotted an orange metabolite pool, what do? [Re: blojo02184]
    #18756865 - 08/25/13 08:16 PM (10 years, 5 months ago)

Quote:

blojo02184 said:
3. Check this out. http://www.shroomery.org/forums/showflat.php/Number/18738488/page/1/fpart/all





Interesting. This leads me to believe the metabolites are from breaking down minerals then, I did notice how my hpoo was absolutely loaded with crystal formations. I was almost going to make a post back then when I was preparing it, I even pulled some rather unique looking ones out to set to the side, though I left em outside and they got washed away in the rain iirc. There was lots of salts and all kinds of minerals of pretty much every color you can imagine, it'd make sense metabolites would likewise be colorful, and hence why it's such a bright orange. I wonder what affect all of these minerals/crystals have on the end product? I thought they might be bad and my manure was overly salty, but I leeched it again and again and just saw no end to the crystal precipitate so ultimately figured it must be a natural part of the mushrooms diet. This also leads me to question whether horses shit gold and diamonds lol.

Quote:

blojo02184 said:
4. Remain calm.





Good advice, good advice. I do have a follow up question if you don't mind though, well, actually two.

It's currently sitting in my kitchen and thus I'm going to have to move them at least in the morning, I don't want to disturb them too much though seeing as I just put them in there. I also did so to give them light because the room they've been in is rather dark, do you think it'd be a bad idea to move it back where it was and/or turn off the light when I go to bed tonight?

The other question is I'm still confused what to do with the metabolites. If they need them to break down the rocks/crystals/minerals wouldn't it be a bad idea to extract them?


Edited by krypto2000 (08/25/13 08:19 PM)


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Offlinekrypto2000
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Re: My mono is almost fully colonized and I just spotted an orange metabolite pool, what do? [Re: krypto2000]
    #18756899 - 08/25/13 08:28 PM (10 years, 5 months ago)

Sorry, but one more question as I just noticed something in that thread. Someone mentioned that these typically only become visible when a substrate is fully colonized. I still see some spots that appear uncolonized, but I'm also aware that the way mycilia diffracts light it can actually be 'fully colonized' and still look black/brown, likewise it can be very small, but due to this diffraction of light that is what gives it the white color. So in other words should I assume my mono is fully colonized and birth it or should I still wait until everything is completely white?


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Offlineblojo02184
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Re: My mono is almost fully colonized and I just spotted an orange metabolite pool, what do? [Re: krypto2000]
    #18756974 - 08/25/13 08:46 PM (10 years, 5 months ago)

It's hard to say. Do you have experience cultivating?

IMO id let it sit where ya left it. IN-direct sunlight from south facing window, preferably second floor so no one looks in Your window and sees a tote full of shrooms hahaha

In that post, they mention that metabolites and enzymes where being produced. Both help with either the defense of the organism or breaking down minerals.
One person, I believe the OP, said that it may help with fighting off other contaminants.
In nature, the mycelial network doesn't get the chance to "pool" enzymes.

Because I hate to say it... If it is contamed, it's already too late.


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Offlinekrypto2000
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Re: My mono is almost fully colonized and I just spotted an orange metabolite pool, what do? [Re: blojo02184]
    #18757095 - 08/25/13 09:07 PM (10 years, 5 months ago)

I've got about... 7 years of experience, but in the past 1-2 years have had terrible luck due to contamination, almost exclusively trichoderma. Before this last grow I cleaned my house top to bottom pretty much. Ironically the only room I didn't clean is the one with a south facing window lol. Like you mentioned though I did suspect it had something to do with the change in light because the lights in this room (the one it was in) are soft whites where as the ones in the kitchen are 'day light' compact fluorescents, so it very well could be a change in the wavelength of light that caused the precipitation of the orange metabolites, and like I also mentioned immediately upon putting it in there I checked the bin rather thoroughly. There's quite a lot of large water droplets (or I'm now wondering if they're diluted metabolites) on the surface so it's somewhat difficult to see, but I pretty much looked that thing up and down to check for any signs of contamination as I've really put my all into this grow. I believe it's good, but time will tell. I guess it's time I clean that last room so it can get some sunlight.


Edited by krypto2000 (08/25/13 09:08 PM)


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Offlinekrypto2000
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Re: My mono is almost fully colonized and I just spotted an orange metabolite pool, what do? [Re: krypto2000]
    #18757143 - 08/25/13 09:18 PM (10 years, 5 months ago)

Whoa, I don't know if I somehow didn't see it before or if it's literally changing that quickly, but I just realized the entire surface is forming primordial knots! It literally seems like for every drop of water sitting underneath the lid there is an equal sized knot on the surface of the substrate. I feel like the bubbles under the lid match up perfectly with the bubbles on the surface to create a magnifying effect concentrating the lights energy to form the knots. I think I just reached mushroom growing enlightenment :shocked: Shit...

edit: I also just realized due to the evaporation of water droples that it's cold on that side because I left the door open to, ironically.. coincidentally... the sun room. That would likewise explain the stress. You can tell soo much just by looking at the condensation and metabolite precipitation. I can practically see the first flush already in there.


Edited by krypto2000 (08/25/13 09:27 PM)


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OfflineSan
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Re: My mono is almost fully colonized and I just spotted an orange metabolite pool, what do? [Re: krypto2000]
    #18757189 - 08/25/13 09:24 PM (10 years, 5 months ago)

That's some deep pseudo-science there, bro.

There might actually be a mathematical correlation, though. The cohesive properties of water are what allow it to form those droplets, and mushrooms are mostly water.


--------------------
Actually not everyone was a noob.  Being a noob is a very new phenomenon.  Many people, the great majority in fact, were simply "beginners", "novices" or "new to mushroom growing".  Being a "noob" is reserved, and in fact created specifically for and by, the newer, much more lame generations coming about.

-Shpongle1


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Offlinekrypto2000
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Re: My mono is almost fully colonized and I just spotted an orange metabolite pool, what do? [Re: San]
    #18757236 - 08/25/13 09:31 PM (10 years, 5 months ago)

Check my edit too. Definitely pseduo-science I'd agree, but like eating mushrooms, growing mushrooms does rely a lot on intuition which often precludes the scientific method, after all intuition is the discovery of something new, something which has been recently discovered by definition has not been verified by multiple trials and there is no control. I do have two bins sitting right next to each other though so there's definitely a measure of variance, and I'm the control, isn't that what intuition is :rofl:.


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Offlineblojo02184
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Re: My mono is almost fully colonized and I just spotted an orange metabolite pool, what do? [Re: krypto2000]
    #18758773 - 08/26/13 08:13 AM (10 years, 5 months ago)

Good things come to those who wait. :smile:

Toss up some pics.


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Offlinekrypto2000
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Re: My mono is almost fully colonized and I just spotted an orange metabolite pool, what do? [Re: blojo02184]
    #18758897 - 08/26/13 09:04 AM (10 years, 5 months ago)

I would, but I don't have a camera atm. Maybe I should just make a new thread to ask this, but it's something I've always taken for granted and never thought about until now. With all of the perspiration underneath the lid what do you guys do with it upon birth?

Normally I'd either fling it all over the room or fan it (along with flinging it all over) right back onto the substrate). After reading that thread though, and having that mini-revelation last night about the droplets potentially acting as a magnifying glass (don't know if that's true of course, it's a theory at best), it's made me realize that literally everything in the tub plays a role and is where it is for a reason. It also could be that the bubbles, while potentially being a magnifying glass, are also simply spore ejaculate :rofl:, and thus throwing them all over and back onto the substrate is likely opening up a pathway for future contaminations.

Personally I have two thoughts on it outside of simply wiping it down with a paper towel 9which always, always results in dropping some of the liquid in the process, since to turn the lid over the dropes inevitably pool up and thus spill all over the substrate or my floor).

The first thought is simply to save it and use it for future projects ala the metabolite thread. There may well be trace metabolites within these droplets, and if there's not they might hold other interesting properties. If the magnification theory holds weight they could be loaded with competing spores, spores specific to your actual grow and region, and thus including the spores (lots of dead spores and metabolized spore by products perhaps too) could act like a vaccination in humans. You inject humans with dead viruses which triggers their immune system to recognize that virus and produce antibodies to defend against it. Likewise this could trigger the mushroom to produce enzymes to defend against potential competitors.

My second thought is one which is rather novel afaik, I've never heard it proposed before. Do not take the lid off or clear off the C02 in one fail swoop, but instead simply open up your mono's holes and plug em with polyfill. Effectively this would allow the CO2 to escape more gradually and also potentially give the mycilium/mushrooms a period of adaptation instead of just throwing them into the open air. I'm not sure what effects this would directly have, but I can think of quite a few scenarios, and likewise the advantage of this, again if any of the above theories are true about the perspiration bubbles, is that they would be left undisturbed other than the sides where the polyfill might touch them. I'm not sure what I am actually going to do on this grow, I'd like to hear some more opinions on the matter and what other shroomerites do, which is why it may just be worth making a new thread as it doesn't relate to the original topic much at all. Thanks for the help so far btw, that thread was very interesting and gave me a lot of potential new insight.


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