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OfflineAmerican Aquariums
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Incubation at increased room temp
    #18754717 - 08/25/13 10:35 AM (10 years, 5 months ago)

Would incubation work the same in an open room if the room temp was kept to 80F-85F? This would be concerning limited privacy where some degree of subtly is required. Also, some of the methods of incubation seem like they'd be fire hazards in smaller spaces.


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Invisiblebudmanman
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Re: Incubation at increased room temp [Re: American Aquariums]
    #18754751 - 08/25/13 10:47 AM (10 years, 5 months ago)

Do not focus on incubation. In fact pretend it does not exist.

The first rule about incubation is, you do not talk about incubation.

The second rule about incubation is,


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OfflinePussyFart
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Re: Incubation at increased room temp [Re: American Aquariums]
    #18754810 - 08/25/13 11:10 AM (10 years, 5 months ago)

Quote:

American Aquariums said:
Would incubation work the same in an open room if the room temp was kept to 80F-85F?



Yup.


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InvisibleSpitballJedi
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Re: Incubation at increased room temp [Re: budmanman]
    #18754820 - 08/25/13 11:14 AM (10 years, 5 months ago)

The only reason I see to use an incubator is if you can't get the room to 70F.

There is no need to make the room 85F.

IMHE, 75F-80F is the sweet spot.

I notice a slow down in growth at 70F but no increase at more than 80F.

As you approach 85F, you can actually increase the possibility of contaminates to take over.

This has been my experience at least. If you use the search feature, you can likely find some science to back up my experience.


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Edited by SpitballJedi (08/25/13 11:15 AM)


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OfflinePussyFart
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Re: Incubation at increased room temp [Re: SpitballJedi]
    #18754878 - 08/25/13 11:37 AM (10 years, 5 months ago)

Quote:

SpitballJedi said:
As you approach 85F, you can actually increase the possibility of contaminates to take over.



Heat does not cause contams, contams cause contams.

The inside of the jar should be sterile, other than mushroom mycellium.

If the jars contam at 85F, they would have contammed at 70F as well.

But if there are no contams present, you should be fine.

The only worry in that case would be the mycellium stalling from temps above 86F.


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InvisibleFrankHorrigan
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Re: Incubation at increased room temp [Re: American Aquariums]
    #18754976 - 08/25/13 12:13 PM (10 years, 5 months ago)

Quote:

American Aquariums said:
Would incubation work the same in an open room if the room temp was kept to 80F-85F? This would be concerning limited privacy where some degree of subtly is required. Also, some of the methods of incubation seem like they'd be fire hazards in smaller spaces.




I mean, yes and no.

An incubator does more than just keep the jars in a temp range, it does so with no dips in the temperature.

Your room temp is bound to jump around and dip, esp at night.

An 80F incubator is so very nice. I highly recommend one.


--------------------

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OfflineAmerican Aquariums
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Re: Incubation at increased room temp [Re: FrankHorrigan]
    #18770360 - 08/28/13 08:27 PM (10 years, 5 months ago)

Can someone also explain to me how much light a dub tub tek requires? These two in mind...
http://www.shroomology.com/topic/8670-6-quart-dub-tub-mist-and-fan-bulk-tek/
http://www.shroomology.com/topic/14-zombie-tubs/
Mexicana or Cube in mind.


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OfflinePussyFart
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Re: Incubation at increased room temp [Re: American Aquariums]
    #18770448 - 08/28/13 08:41 PM (10 years, 5 months ago)

Quote:

American Aquariums said:
Can someone also explain to me how much light a dub tub tek requires? These two in mind...
http://www.shroomology.com/topic/8670-6-quart-dub-tub-mist-and-fan-bulk-tek/
http://www.shroomology.com/topic/14-zombie-tubs/
Mexicana or Cube in mind.



Lighting Requirements of Mushrooms

Some mushrooms, such as the Agaricus species commonly found in grocery stores require no light at all. However, those commonly grown by hobbyists, such as Pleurotus ostreatus (Oyster Mushrooms), Lentinus enodes (Shiitake), Psilocybe cubensis, a hallucinogenic mushroom, and Hericium erinaceus (Lion's Mane) all require light to produce abundant, normal sized fruits. Experience has taught us that the light best suited for primordia formation and the development of fruitbodies is bright light with a color temperature of 5,000 Kelvin to 7,000 Kelvin. Fortunately, this type of light is easily obtainable at your local home improvement center in the form of fluorescent fixtures. For a small terrarium as described in this chapter, a single CFL (compact fluorescent) that screws into a standard light bulb socket will work very well. These can often be found in grocery and drug stores in every neighborhood. 15 watt CFLs will do the job well, but the package will probably have a large 60 stamped on it, indicating they produce light "equivalent" to a 60 watt incandescent light bulb. They're referring to lumens of output, not the frequency. Incandescent light bulbs are the worst possible choice for growing mushrooms, since they emit a 'red' light in the 3,000 Kelvin color temperature range.

The higher the color temperature, expressed in Kelvin, the closer to the 'blue' end of the spectrum the emitted light is. The lower the color temperature the 'redder' the light is. If you have a choice of fluorescent lamps, purchase those labeled 'daylight' since these have a somewhat higher color temperature than cool white. Daylight, sometimes called 'natural daylight' fluorescent tubes generally emit light in the 6,500 Kelvin range, while cool white fluorescent emits light at around 5,000 Kelvin.
If you have several terrariums stacked or otherwise near each other, you can use larger 2 to 4 tube fluorescent fixtures. These come in 48" and 96" lengths. Place the fluorescent lamps as close as you can get them to your terrariums without causing excessive heating. Species such as Shiitake and Oyster mushrooms prefer to fruit at temperatures in the upper 50's to mid 60's Fahrenheit (15C to 20C), while Psilocybe cubensis prefers to fruit at a temperature in the mid 70s to about 80 Fahrenheit (23C to 27C)
Most mushroom species don't mind a slightly warmer temperature during daytime than at night, so if your grow room is a bit colder than the temperature ranges given above, a little warming from your lights during the daytime won't hurt at all, provided you don't let the air in your terrarium get too dry. For cakes, try to keep the humidity above 95%.
Cased substrates are a bit more forgiving, but still try to keep your humidity above 90%. 12 hours on, 12 hours off has proved to be a great combination over a wide range of species. Of course, if you have a bright window near your terrarium, that will suffice, but direct sunlight for more than a few minutes per day should be avoided.
Disregard outdated advice in old books which is constantly repeated on the internet to colonize mushroom substrates in total darkness. Experience and rigorous peer reviewed studies have proved that exposure to low level ambient indoor lighting during spawn run and substrate colonizing will speed up the process, leading to full colonization up to a few days earlier than the same substrate would if colonized in darkness. In addition, mushroom mycelium develops a day/night circadian rhythm, so exposure to light from day of inoculation sets this process in motion, leading to earlier fruiting and harvest.


Source: http://www.mushroomvideos.com/Terrarium-Tek


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Offlineheratogwea
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Re: Incubation at increased room temp [Re: PussyFart]
    #18770606 - 08/28/13 09:13 PM (10 years, 5 months ago)

Keep mine on my attic stairs. 83 max during the day and 74 lowest at night. They also get some very nice ambient light from the windows up top.

Here one of them 10 days after inoculation:



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OfflinePsilicon
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Re: Incubation at increased room temp [Re: PussyFart]
    #18770611 - 08/28/13 09:14 PM (10 years, 5 months ago)

Quote:

Notahacker420 said:
Heat does not cause contams, contams cause contams.

The inside of the jar should be sterile, other than mushroom mycellium.

If the jars contam at 85F, they would have contammed at 70F as well.





I'm not so sure about this.  It's been shown over and over that you can get a flush with a badly bacterially contaminated cake, and this ability to coexist means that there will be a gradient of contamination, with each species fighting for nutrients but neither winning outright where both exist.  Sure, it's possible (and even easy) to destroy all bacteria in your jars, but I'm not confident that any bacteria means serious contamination.  I think it might turn out that keeping your jars cool thwarts many bacteria, especially spore producers like bacillus, since they have actually been known to sporulate and lyse at 73F while cubensis does pretty well at that temperature.

In Denver the boiling point of water is 203F, while where you live it's actually right where most people expect it to be, at 212F.  Those extra 9 degrees make one hell of a difference when you consider that at 194F you still have 3% of bacillus subtilis spores surviving, but despite that people in Colorado still sometimes seem to be able to use steam sterilization for the PF Tek with some success.  I think the reason is how warm the jars are kept during colonization.

I also think this is why there's so much anecdotal evidence of bacterial contamination at warmer temps for newbs, but for the old hands (every single one of whom uses a pressure cooker) incubation works quite well.


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OfflineAmerican Aquariums
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Re: Incubation at increased room temp [Re: Psilicon]
    #18770743 - 08/28/13 09:37 PM (10 years, 5 months ago)

About the light... How many hours a day, if leaving them on a windowsill all day wasn't possible?


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OfflinePsilicon
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Re: Incubation at increased room temp [Re: American Aquariums]
    #18770759 - 08/28/13 09:39 PM (10 years, 5 months ago)

Twelve-ish hours a day is ideal.  You want to emulate the regions these mushrooms grow in, right?


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OfflineAmerican Aquariums
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Re: Incubation at increased room temp [Re: Psilicon]
    #18770970 - 08/28/13 10:14 PM (10 years, 5 months ago)

Should any specific 12 hours be observed? Or need it only be consistent?


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OfflinePussyFart
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Re: Incubation at increased room temp [Re: American Aquariums]
    #18771006 - 08/28/13 10:22 PM (10 years, 5 months ago)

12/12 constant.


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OfflineAmerican Aquariums
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Re: Incubation at increased room temp [Re: PussyFart]
    #18771011 - 08/28/13 10:24 PM (10 years, 5 months ago)

Is there a way to have small lights turn themselves on and off on schedule?


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OfflinePussyFart
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Re: Incubation at increased room temp [Re: American Aquariums]
    #18771072 - 08/28/13 10:38 PM (10 years, 5 months ago)

A timer......


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