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OfflineUltron
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Need advice on germination
    #18752986 - 08/24/13 08:31 PM (10 years, 5 months ago)

Started some b+ in some pf tek jars im incubating on a shelf at around 80 degrees Fahrenheit.  I used multispore inculcation and i got my spore at a local headshop. I used a pressure cooker to sterilize and let my jars cool over night. Its been about 2 1/2 weeks and see no growth of contams or mycelium. Any advice on how i should proceed should i start over or should i let the jars sit a little longer. When i look at the syringe i can see small bits of spores i believe. any advice or tips would be appreciated  i'am very interested in mycology and would love to learn as much as possible :laugh:


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Edited by Ultron (08/24/13 09:07 PM)


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Invisibleuncle_rico
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Re: Need advice on germination [Re: Ultron]
    #18753060 - 08/24/13 08:57 PM (10 years, 5 months ago)

2 1/2 months ?

Forget about it.  sorry.

Start over.  get a spore syringe from a vendor here and you'll have lots to show and tell in 2 1/2 months.


good luck


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OfflineStromriderM
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Re: Need advice on germination [Re: uncle_rico]
    #18753075 - 08/24/13 08:59 PM (10 years, 5 months ago)

Your local head shop sold you a bunk syringe


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OfflineUltron
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Re: Need advice on germination [Re: uncle_rico]
    #18753077 - 08/24/13 09:00 PM (10 years, 5 months ago)

typo on my end sorry 2 1/2 weeks :P  even if i have to start over i still gained the experience :smile:


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OfflineStromriderM
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Re: Need advice on germination [Re: Ultron]
    #18753091 - 08/24/13 09:03 PM (10 years, 5 months ago)

That sounds a little better but still a little much. If you don't see growth in the next week get a new syringe from a shroomery vendor and start over.


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OfflineUltron
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Re: Need advice on germination [Re: Stromrider]
    #18753098 - 08/24/13 09:05 PM (10 years, 5 months ago)

ok cool do you have any experience with liquid culture i was thinking of doing a lc next time and doing a mini pf tek and then going in to a monotub or greenhouse after? :smile:


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Re: Need advice on germination [Re: Ultron]
    #18753110 - 08/24/13 09:09 PM (10 years, 5 months ago)

OK... 2 1/2 weeks.

still not lookn really good.

most guys will see growth in 3 to 5 days .. and jars could possibly be finishing up in 2 1/2 weeks.

watch em but don't expect too much.


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OfflineUltron
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Re: Need advice on germination [Re: uncle_rico]
    #18753125 - 08/24/13 09:13 PM (10 years, 5 months ago)

i have patience every attempt is a new experience i expect nothing but hope for results :smile:


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My journal of grow logs


Edited by Ultron (08/24/13 09:26 PM)


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OfflineStromriderM
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Re: Need advice on germination [Re: Ultron]
    #18754069 - 08/25/13 04:36 AM (10 years, 5 months ago)

Quote:

jonathan9301 said:
ok cool do you have any experience with liquid culture i was thinking of doing a lc next time and doing a mini pf tek and then going in to a monotub or greenhouse after? :smile:




Yeah just forget about LC. It is a bad idea


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Re: Need advice on germination [Re: Stromrider]
    #18754322 - 08/25/13 07:42 AM (10 years, 5 months ago)

Quote:

psillyshroomer said:
Quote:

jonathan9301 said:
ok cool do you have any experience with liquid culture i was thinking of doing a lc next time and doing a mini pf tek and then going in to a monotub or greenhouse after? :smile:




Yeah just forget about LC. It is a bad idea[/quote]

LC's are not a bad idea!! in fact, if you get a good one (easily done) you will have tons of inoculant to play with. I've had plenty of successful grows using LCs, you just have to test it first. Like I did here http://www.shroomery.org/forums/showflat.php/Number/17778775]


Edited by billb (08/25/13 07:47 AM)


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Offlineblueconfusion
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Re: Need advice on germination [Re: billb]
    #18754356 - 08/25/13 08:01 AM (10 years, 5 months ago)

Yeah I wouldn't start with an lc either get pf tek down first and buy spores from vendor simplicity is the beat way to start lc's can contaminate if not done right then youve lost everything dont put all your eggs in one basket pf tek pf tek pf tek then move up


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OfflineStromriderM
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Re: Need advice on germination [Re: blueconfusion]
    #18754415 - 08/25/13 08:34 AM (10 years, 5 months ago)

Quote:

blueconfusion said:
Yeah I wouldn't start with an lc either get pf tek down first and buy spores from vendor simplicity is the beat way to start lc's can contaminate if not done right then youve lost everything dont put all your eggs in one basket pf tek pf tek pf tek then move up




I agree. I guess I should have said lc is a bad idea for a newb


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Re: Need advice on germination [Re: Stromrider]
    #18754439 - 08/25/13 08:42 AM (10 years, 5 months ago)

I personally dont like lc's with ms either yeah it gives you more to work with but who knows what it is isolates to lc's are the way to go


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Re: Need advice on germination [Re: blueconfusion]
    #18754444 - 08/25/13 08:45 AM (10 years, 5 months ago)

And even then glc is prolly better to start with so you can spot contams easier


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Re: Need advice on germination [Re: blueconfusion]
    #18754446 - 08/25/13 08:45 AM (10 years, 5 months ago)

I agree with you. There are much better ways of doing things than Lc. Some people really get offended when they hear you tell people Lc is a bad idea. Using agar to grow a clean culture or clone then transfer that to grain and then doing g2g to increase your culture stock is the way to go imo


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Re: Need advice on germination [Re: Stromrider]
    #18754448 - 08/25/13 08:46 AM (10 years, 5 months ago)

Agreed


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OfflinePussyFart
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Re: Need advice on germination [Re: blueconfusion]
    #18754548 - 08/25/13 09:25 AM (10 years, 5 months ago)

LCs are sketchy as fuck when not made from an agar wedge.


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OfflineUltron
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Re: Need advice on germination [Re: PussyFart]
    #18754711 - 08/25/13 10:33 AM (10 years, 5 months ago)

ok cool i plan on trying everything so liquid cultures are gonna happen sometime and i was gonna do pf tek till i got it down and move on from there. :smile:  thanks for all the info


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OfflineUltron
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Re: Need advice on germination [Re: Ultron]
    #18754726 - 08/25/13 10:38 AM (10 years, 5 months ago)

and i wasn't planning on doing a multispore lc i was gonna do a  needle biopsy of some mushroom tissue and create a lc off that for storage purposes and a spore print for prolonged storage


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Re: Need advice on germination [Re: Ultron]
    #18754804 - 08/25/13 11:08 AM (10 years, 5 months ago)

Making an LC from tissue carries the same risk as making one from spores.

The only good way to make an LC is from a clean agar wedge, and even then it's like you are working backwards.

The best bet is to experiment with LCs, but never count on them...Agar is the way to go buddy.


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Re: Need advice on germination [Re: PussyFart]
    #18795050 - 09/03/13 04:09 PM (10 years, 4 months ago)

I lost all my work on an incredible strain due to an lc but didnt know it until I wasted 2 grows losing both times to cobweb do glc or agar


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Re: Need advice on germination [Re: blueconfusion]
    #18796384 - 09/03/13 08:52 PM (10 years, 4 months ago)

OP i was wondering if you had any updates on how the jars were growing its been 6 days and no signs of growth yet for me same strain.


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Re: Need advice on germination [Re: shadow6211]
    #18796750 - 09/03/13 09:50 PM (10 years, 4 months ago)

Quote:

shadow6211 said:
OP i was wondering if you had any updates on how the jars were growing its been 6 days and no signs of growth yet for me same strain.




The "strain" has nothing to do with it. Genetics from one cube grow to the next can be different even the label they stuck on the syringe is the same as the next guy's.


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OfflineUltron
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Re: Need advice on germination [Re: FooMan]
    #18800077 - 09/04/13 04:58 PM (10 years, 4 months ago)

ya heres a update after three and a half weeks no growth i decided to get new spores. I believe the spores i got were bunk. I got a money order today so i could order some spores from ralphsters. I plan on doing a liquid culture next time but im  just doing that as a side project my main project is gonna be another multispore pf tek. :laugh:


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OfflineUltron
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Re: Need advice on germination [Re: shadow6211]
    #18800089 - 09/04/13 05:00 PM (10 years, 4 months ago)

Ya my spores were probably bunk 6 days isn't very long hopefully they will show some signs soon. Good luck in your endeavors


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Re: Need advice on germination [Re: Ultron]
    #18800821 - 09/04/13 08:16 PM (10 years, 4 months ago)

Thanks jonathan good luck in your next syringe from what i heard patience is key so thats what im trying to gain!  When you get your next jars going post some pics of progress.


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Re: Need advice on germination [Re: shadow6211]
    #18800914 - 09/04/13 08:41 PM (10 years, 4 months ago)

Quote:

shadow6211 said:
Thanks jonathan good luck in your next syringe from what i heard patience is key so thats what im trying to gain!  When you get your next jars going post some pics of progress.



I will post pics as soon as i get my spores :smile: if trying to learn patience practice meditation it works wonders


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Re: Need advice on germination [Re: Ultron]
    #18867910 - 09/20/13 02:04 PM (10 years, 4 months ago)



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