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Offlinenimbus8
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Registered: 09/16/12
Posts: 91
Last seen: 3 years, 3 months
Flame sterilization
    #18750517 - 08/24/13 08:31 AM (10 years, 5 months ago)

When doing flame sterilization how long should one wait after flaming the needle. Does a hot needle kill any spores coming through it?

Additionally is alcohol necessary after flame sterilization?


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Offlinegoldenlearner
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Registered: 08/22/13
Posts: 9
Loc: South East US
Last seen: 10 years, 5 months
Re: Flame sterilization [Re: nimbus8]
    #18750540 - 08/24/13 08:38 AM (10 years, 5 months ago)

My friend's last batch was noc'd up using flame sterilization on the needle until it turned red. He waited 20 seconds after each flame, and they colonized beautifully without contam.


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
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Trusted Cultivator
Re: Flame sterilization [Re: goldenlearner]
    #18750707 - 08/24/13 09:31 AM (10 years, 5 months ago)

Flame it until red hot, then expell a drop of spore solution or 2.

The first drop(s) will cool the needle and the rest will flow contam free.


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InvisibleSpitballJedi
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Registered: 10/13/12
Posts: 8,598
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Re: Flame sterilization [Re: PussyFart]
    #18750949 - 08/24/13 10:40 AM (10 years, 5 months ago)

:whathesaid:

...and don't wipe it with alcohol after flaming.


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