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Offlinemushrume man
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GB specific gloves
    #18750252 - 08/24/13 05:36 AM (10 years, 5 months ago)




Anyone use these type of gloves in the boxes?

A quick search brings up a black pair for $260


Maybe this will work


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Offlinegoldenlearner
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Re: GB specific gloves [Re: mushrume man]
    #18750637 - 08/24/13 09:07 AM (10 years, 5 months ago)

Try this link: Extra long rubber gloves $17.99/ free shipping

They are cheaper.... A LOT cheaper. And will work just as effectively.


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OfflineBloodKil
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Re: GB specific gloves [Re: goldenlearner]
    #18750665 - 08/24/13 09:17 AM (10 years, 5 months ago)

If you search around ebay you can get the Atlas 26" gloves (very heavy duty) for ~9 bucks shipped a pair. (Or you can be lazy and get them here for 13 bucks shipped)

Edit...  If you search my posts yesterday, you'll be able to ee these very gloves in my glove box...  I love them.


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Offlineforrest
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Re: GB specific gloves [Re: goldenlearner]
    #18750669 - 08/24/13 09:18 AM (10 years, 5 months ago)

when i first tried to make a glovebox i searched wide and far for long gloves. but i found out that using the thicker plastic, or ones that don;t fit snug, make it so much more of a hassle, and that you can work less fast and precise, that it in the end is better to use the thin latex ones.


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OfflinePussyFart
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Re: GB specific gloves [Re: forrest]
    #18750677 - 08/24/13 09:24 AM (10 years, 5 months ago)

Still air boxes are better than glove boxes for this hobby.

The gloves should be attached to you, and not the box.

It makes flaming your tools between jars possible, and just works better than a GB.


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InvisibleFrankHorrigan
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Re: GB specific gloves [Re: mushrume man]
    #18750768 - 08/24/13 09:47 AM (10 years, 5 months ago)

Quote:

mushrume man said:
Anyone use these type of gloves in the boxes?

A quick search brings up a black pair for $260





What are you doing, making meth as well? Two hundred and sixty fucking dollars?? :lmafo:

Dude, just get a box of disposable nitrile gloves and a disposable painters coverall for $20 total.

You'll have gloves for months and months.


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Offlineblojo02184
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Re: GB specific gloves [Re: FrankHorrigan]
    #18750915 - 08/24/13 10:30 AM (10 years, 5 months ago)

He's not making meth, he's grabbing the cow Patties right outta the cow with them gloves! holy Shit.


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Offlinemushrume man
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Re: GB specific gloves [Re: blojo02184]
    #18751849 - 08/24/13 02:40 PM (10 years, 5 months ago)

Quote:

Notahacker420 said:
Still air boxes are better than glove boxes for this hobby.

The gloves should be attached to you, and not the box.

It makes flaming your tools between jars possible, and just works better than a GB.




To each his own, this is what I have now SAB. Just like the idea of going in and out and not needing to recoat gloves with iso. Also a butane torch can be used in a glove box of the right configuration.

Quote:

FrankHorrigan said:
Quote:

mushrume man said:
Anyone use these type of gloves in the boxes?

A quick search brings up a black pair for $260





What are you doing, making meth as well? Two hundred and sixty fucking dollars?? :lmafo:

Dude, just get a box of disposable nitrile gloves and a disposable painters coverall for $20 total.

You'll have gloves for months and months.




Lol I know it's ridiculous but if they are going to last years then it might even out, and work better?

Quote:

blojo02184 said:
He's not making meth, he's grabbing the cow Patties right outta the cow with them gloves! holy Shit.



:psycow:  :rofl2:

BTW neither of those links worked


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OfflinePussyFart
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Re: GB specific gloves [Re: mushrume man]
    #18751925 - 08/24/13 02:59 PM (10 years, 5 months ago)

Quote:

mushrume man said:
To each his own, this is what I have now SAB. Just like the idea of going in and out and not needing to recoat gloves with iso. Also a butane torch can be used in a glove box of the right configuration.



I do not recoat my gloves when I go in and out of my box, and I never have problems.

What you are looking for is a still air environment, and with a butane torch that is constantly generating heat currents because it is being worked so often, this is never a good thing.

Plus there is that possibility of melting/warping the plastic, and if you use alcohol in the box forget it....that is an accident waiting to happen.

I agree to each his own, but unless you work really hard to seal off the lid of a glovebox, air is always going to pass in and out thru the imperfect seal, so this will negate the "still air" environment yet again.

Just sayin....try and keep an open mind....


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OfflineBloodKil
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Re: GB specific gloves [Re: PussyFart]
    #18752006 - 08/24/13 03:20 PM (10 years, 5 months ago)

Quote:

Notahacker420 said:
I agree to each his own, but unless you work really hard to seal off the lid of a glovebox, air is always going to pass in and out thru the imperfect seal, so this will negate the "still air" environment yet again.

Just sayin....try and keep an open mind....



:whathesaid:

I fucking love my glove box and have had nothing but the best results from it.  That said...  If you do not have a properly sealed and sanitized gb, you will  likely have more issues than a simple sab. 

If your adamant about taking the step into gb territory, please make sure you have a extremely well sealed area, you sanitize with appropriate materials, and you allow a settle time after each side ani cleaning with said materials before working inside of it. 

I've had the pleasure of a 0% contamination ratio within my glove box from non wild specimens, but I have taken the time to create a almost completely sealed environment with it (the only entry way is the zipper in the back that is covered by a polyethylene flap) and take the time to clean with clorox wipes and allow a 20+minute settle time between work after I have cleaned it.  This has allowed me to flame sterilize my equipment inside of my gb, however unless all steps are taken as above, you should expect the same or possibly lower ratio of contams from what you would get with a much easier to work with sab.

(I love my gb to death, but wish to provide a devils advocate for those who are not up to the tasks involved in creating a use able environment in one)


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InvisibleFrankHorrigan
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Re: GB specific gloves [Re: mushrume man]
    #18752185 - 08/24/13 04:03 PM (10 years, 5 months ago)

Taking a look, those gloves are way too bulky to work with.

You need some degree of precision while doing sterile work. That means tight-fitting gloves are going to be necessary. Imagine those bulky things moving around while you manipulate a scalpel with one hand and a petri dish with another.  Even syringe inoculations would prove clumsy I betcha.

Use gloves that are intended for careful work, such as medical exam gloves.


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Offlinemushrume man
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Re: GB specific gloves [Re: BloodKil]
    #18752370 - 08/24/13 05:05 PM (10 years, 5 months ago)

Quote:

Notahacker420 said:
I do not recoat my gloves when I go in and out of my box, and I never have problems.

What you are looking for is a still air environment, and with a butane torch that is constantly generating heat currents because it is being worked so often, this is never a good thing.

Plus there is that possibility of melting/warping the plastic, and if you use alcohol in the box forget it....that is an accident waiting to happen.

I agree to each his own, but unless you work really hard to seal off the lid of a glovebox, air is always going to pass in and out thru the imperfect seal, so this will negate the "still air" environment yet again.

Just sayin....try and keep an open mind....




I feel ya bro. Don't know why the flame currents didn't cross my mind. But could you clear up something for me, if the imperfect seal negates the still air then what about the SAB which has holes for your gloved hands?

BTW what does your house look like inside? I have mold in corners of most rooms and it makes me paranoid in these situations.

Quote:

FrankHorrigan said:
Taking a look, those gloves are way too bulky to work with.

You need some degree of precision while doing sterile work. That means tight-fitting gloves are going to be necessary. Imagine those bulky things moving around while you manipulate a scalpel with one hand and a petri dish with another.  Even syringe inoculations would prove clumsy I betcha.

Use gloves that are intended for careful work, such as medical exam gloves.




I am all for dexterity. Just had to buy the small size gloves the other day because of the irritating 1/4 of nitrile that hangs off the tips of my fingers.
There are gloves of other composition on the site, I'm guessing could be thinner. But generally these are intended for careful work in a glove box right? What work in a GB would not be careful?

Sorry if I sound naive, just want to wrap my head around this fully. What makes a SAB more reliable than a glovebox with the same amount of unsealed orifices? Around the opening or other pin holes. The air in either should be still until your hands are inserted.




Quote:

BloodKil said:
If you search around ebay you can get the Atlas 26" gloves (very heavy duty) for ~9 bucks shipped a pair. (Or you can be lazy and get them here for 13 bucks shipped)

Edit...  If you search my posts yesterday, you'll be able to see these very gloves in my glove box...  I love them.




Thanks, I will most likely get those when I dive into this project


I appreciate all of your experienced input on this :seriousthankyou:


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InvisibleSpitballJedi
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Re: GB specific gloves [Re: FrankHorrigan]
    #18752393 - 08/24/13 05:13 PM (10 years, 5 months ago)

I get a great deal of satisfaction watching people be so insistent on making things more complicated than they need to be. Mostly because I was right there too, not too long ago.

I can't even calculate how much money and time I've wasted trying to do what can be done simply and cheaply.

OP, I understand your your feeling the need for precaution, but a SAB is really better than a glove-box.

Putting your hands in and out of the holes of a SAB does not create as much turbulance as taking them in and out of the gloves. The gloves cause suction and pressure.

If you insist on a GB, at least promise me you won't use a flame inside of it with anything flammable. You could catch yourself on fire.


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Offlinetropicalfrenzy
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Re: GB specific gloves [Re: SpitballJedi]
    #18752436 - 08/24/13 05:23 PM (10 years, 5 months ago)

Quote:

blojo02184 said:
He's not making meth, he's grabbing the cow Patties right outta the cow with them gloves! holy Shit.




waaaahahahahahah! :lol: that deserved a rating.

Quote:

SpitballJedi said:

I can't even calculate how much money and time I've wasted trying to do what can be done simply and cheaply.

OP, I understand your your feeling the need for precaution, but a SAB is really better than a glove-box.





^^^THIS!^^^


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OfflineBloodKil
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Re: GB specific gloves [Re: SpitballJedi]
    #18752465 - 08/24/13 05:36 PM (10 years, 5 months ago)

Quote:

SpitballJedi said:
OP, I understand your your feeling the need for precaution, but a SAB is really better than a glove-box.

Putting your hands in and out of the holes of a SAB does not create as much turbulance as taking them in and out of the gloves. The gloves cause suction and pressure.

If you insist on a GB, at least promise me you won't use a flame inside of it with anything flammable. You could catch yourself on fire.




:facepalm3:

Sorry but in reality most of this doesn't make much sense....  In a properly built gb you don't have the suction draw issue (he'll even in my half asked cheap version I'm fine)

The in and out of a sab allows a much greater risk than a proper gb ever could.

As far as flaming inside of it, it's as simple as using non flammable, non combustible agents and allowing for a proper settle time to eliminate airborne contams that would be effected bt the heat currants...  I'm not knocking a sab by any means as I'm sure it is easier and safer by a multitude for most peeps, but a properly constructed and sanitized gb will be better by leaps and bounds...


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Offlinemushrume man
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! [Re: SpitballJedi]
    #18752496 - 08/24/13 05:46 PM (10 years, 5 months ago)

Quote:

SpitballJedi said:
I can't even calculate how much money and time I've wasted trying to do what can be done simply and cheaply.

OP, I understand your your feeling the need for precaution, but a SAB is really better than a glove-box.

Putting your hands in and out of the holes of a SAB does not create as much turbulance as taking them in and out of the gloves. The gloves cause suction and pressure.

If you insist on a GB, at least promise me you won't use a flame inside of it with anything flammable. You could catch yourself on fire.




Thanks this is what I was guessing. The glove box should be air tight do to pressure changes.

Don't worry, not going to set myself on fire. I'm use to working around flammables, liquid and gas. I would stick to bleach if using a flame in a closed environment.

I am considering modifying my procedures in a way to get rid of the need for a SAB or GB. Except for isolation.


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InvisibleSpitballJedi
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Re: GB specific gloves [Re: BloodKil]
    #18752593 - 08/24/13 06:22 PM (10 years, 5 months ago)

Quote:

BloodKil said:
In a properly built gb you don't have the suction draw issue (he'll even in my half asked cheap version I'm fine)

The in and out of a sab allows a much greater risk than a proper gb ever could.




The key words there are "properly built". Even then, we are talking about mycology, not gene splicing. How sterile does it really need to be? Apparently as sterile as a SAB provides. We don't even need sterile workspace, we just need the air to be still. Glove boxes are overkill for this hobby.

Quote:

BloodKil said:
...a properly constructed and sanitized gb will be better by leaps and bounds...




See above retort. At best, at least for this hobby, a GB gives an imperceptible degree safety over a SAB. It's definitely not better, because it traps you in and restricts your movements.

My point is, if you pay attention to your technique and understand what and why you are doing, then you can eliminate some of the cost and complications.

I wish I had the skill to better impart this wisdom, but it's something most have to learn on there own, like I did.

The GB vs SAB debate has been run in the ground. No new info will be submitted here. It's all been said many many times. It's boring to debate.


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OfflinePussyFart
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Re: GB specific gloves [Re: BloodKil]
    #18752828 - 08/24/13 07:37 PM (10 years, 5 months ago)

Quote:

BloodKil said:
The in and out of a sab allows a much greater risk than a proper gb ever could.



After moving in and out, we allow the air to settle before going to work.

In a glovebox, every time you move your arms air gets sucked in and out of the imperfect seals.


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InvisibleFrankHorrigan
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Re: GB specific gloves [Re: PussyFart]
    #18752946 - 08/24/13 08:12 PM (10 years, 5 months ago)

I don't let the air settle at all nowadays when I'm working in my SAB.

The key to perfect sab success is using it in a draft-free room and learning how to use good sterile technique.

The draft free room is extra important. If you don't have air moving around you, contams will settle outside the box (for the most part) and give you a whole lot of space to work and make mistakes.

I even do my work with the lid off in a draft free space. I have not had a single contam on my agar in months.

A little food for thought :thumbup:


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Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Edited by FrankHorrigan (08/24/13 09:04 PM)


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