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OfflineKizzle
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Registered: 08/30/11
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Re: Poor mans flow 'hood' idea. [Re: rew736]
    #18748994 - 08/23/13 08:56 PM (10 years, 5 months ago)

Quote:

thats exactly what it is. the public schools are the worst.



I seem to recall learning about fluid dynamics on a very basic level. You can't honestly expect them to teach the practical knowledge to design an air flow system in high school. We can't see things like mold spores or even detect the minute air currents required to move them. Until you've actually studied in more detail things like that the prospect of preventing contaminants from settling into the jars with an updraft seems naturally intuitive.


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OfflineBloodKil
Mangler av era mödrar slida
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Re: Poor mans flow 'hood' idea. [Re: Kizzle]
    #18749135 - 08/23/13 09:29 PM (10 years, 5 months ago)

Agar is in the pc right now...  I'll get the plates poured tonight and then use my fanhood (lol) in the morning...

I'll just be running a single plate under the fanhood with the plate lifted approximately 1" for 10 seconds. (As that's about how high I lift my lids when doing transfers.

I'll have another plate in my glove box that I will do the same for but just for shits and giggles will be holding the lid up for a full minute as I feel the fanhood needs a handicap...  (I could probably leave the lid off in my gb as I have yet to have a contamed plate that wasn't from a wild specimen)

Results will likely be in before next weekend.



Edit..

Fan to be used...


It's a 4 speed (either direction) 56"

As this was your idea krypto ill let you decide which speed I use...  (as a disclaimer, I'm not allowed to have it on high (setting 4) when my gf is over as it fucks up her hair...  and though I cannot really feel air currents in reverse low, it provides a nice amount of air movement on forward low...) so 1, 2, 3, or 4?


Edited by BloodKil (08/23/13 09:45 PM)


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OfflinePsilosopherr
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Re: Poor mans flow 'hood' idea. [Re: BloodKil]
    #18749358 - 08/23/13 10:24 PM (10 years, 5 months ago)

damn dude. You should either have a couple more plates, or do multiple trials to get more accurate results.

if you feel like it


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OfflineBloodKil
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Re: Poor mans flow 'hood' idea. [Re: Psilosopherr]
    #18749607 - 08/23/13 11:29 PM (10 years, 5 months ago)

I gues ill run 2 under the fan, but to be honest im not sure theres a point to it...  If there are no contams ill try more plates...

I just poured 20, but don't see a reason to waste more than 2 if it contams.  I have a 0% contamination rate in my 20$ glove box...  If this contams even once it's not worth the time to try again IMO as the people using it would spend more money on wasted plates and/or agar over the course of a single isolate than what it would take to duplicate a gb.  (And this is supposed to be a flow hood alternative)



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Offlinekrypto2000
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Re: Poor mans flow 'hood' idea. [Re: BloodKil]
    #18749776 - 08/24/13 12:23 AM (10 years, 5 months ago)

Well I agree with you guys that it likely won't work, but I'd suggest doing a single plate with the fan off and then one with the fan on as I wasn't originally promoting this as better than GB or flowhood, just an alternative to working in the open air if you already do not have either. I never tested it myself because I have a GB.

The fan speed would depend on the size of the room as I figured it, you'd want it at a speed that is relative to the size of the room, so it's kind of up to you. I'd suggest holding you hand a few inches above the work/table and find a speed which you can barely feel the upward current and then drop it one below that, so probably a 1 or 2 on your fan (assuming 1 is slow and 4 is fast). I still think a cigarette or a source of smoke would be best to figure that out as you can actually see the turbulence, ideally the smoke would go strait up. You could also use a candle or lighter and just watch how much the flame flickers.


Edited by krypto2000 (08/24/13 12:24 AM)


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InvisibleSpitballJedi
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Re: Poor mans flow 'hood' idea. [Re: krypto2000]
    #18750788 - 08/24/13 09:51 AM (10 years, 5 months ago)

You don't even want the fan on when working in a SAB.


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Offlinekrypto2000
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Re: Poor mans flow 'hood' idea. [Re: SpitballJedi]
    #18756529 - 08/25/13 07:02 PM (10 years, 5 months ago)

Yeah I typically turn my house into a still air box when I work in my still air box :tongue:.


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OfflineBloodKil
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Re: Poor mans flow 'hood' idea. [Re: krypto2000]
    #18756686 - 08/25/13 07:30 PM (10 years, 5 months ago)

I ended up doing one plate @ speed 1 and one plate at speed 2. (A few hours ago..)

Starting tomorrow. (~21 hours from now) ill take daily pics for at least 1 week as contamination if present should show itself well within that time period.

Pics will be uploaded after finished.


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OfflineBittrBuffalo
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Re: Poor mans flow 'hood' idea. [Re: krypto2000]
    #18756877 - 08/25/13 08:20 PM (10 years, 5 months ago)

Admittedly I haven't read this entire thread, so if someone's already mentioned it, I apologize. Everyone's already explained why blowing shit around the entire room with moving air is exactly the opposite of what you're trying to do during inoculation. That's why glove boxes are used--to prevent this from happening. I see where your brain was going with this, but in reality it actually causes the problem rather than preventing it.

For me, the oven-door tek has worked in terms of a jury-rigged flow hood. I'm sure it falls in the "experimental" category and is therefore not necessarily recommended by anyone anywhere. I don't know what you're looking to inoculate, but oven-door is probably better suited for jars. I've also used a sort of open(ish) air method with the little alcohol soaked sponge that you lay over your inoculation point and stab your needle through. So far so good on that one, too.

So that's neither here nor there. Teks that are 10 years old aren't necessarily bad. They're still around because they work. The reason, I suppose, that people will go to great lengths to find an alternative to open-air inoculation is not because they want to make extra work for themselves for no reason, but because open-air has a high failure rate and they want to prevent that.


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Disclaimer: This post is a work of fiction, provided for entertainment purposes only. Any resemblance to actual persons or events, past or present, is strictly coincidental. All celebrity voices are impersonated. If you begin your ID request with, "I just ate a bunch of these mushrooms…should I not have done that?" I'm just gonna sit back and watch Darwin at work.:mushroom2:


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OfflineBittrBuffalo
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Re: Poor mans flow 'hood' idea. [Re: krypto2000]
    #18756890 - 08/25/13 08:25 PM (10 years, 5 months ago)

I mean, that's the point, right? To figure out something that works as an alternative to a flow hood?


--------------------
Disclaimer: This post is a work of fiction, provided for entertainment purposes only. Any resemblance to actual persons or events, past or present, is strictly coincidental. All celebrity voices are impersonated. If you begin your ID request with, "I just ate a bunch of these mushrooms…should I not have done that?" I'm just gonna sit back and watch Darwin at work.:mushroom2:


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Offlinekrypto2000
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Re: Poor mans flow 'hood' idea. [Re: BittrBuffalo]
    #18757478 - 08/25/13 10:19 PM (10 years, 5 months ago)

I wouldn't say an alternative, if you have or can build a flow hood you should, simple as that, it's better. I was merely proposing the idea for people just starting out if you have nothing and cannot afford even a GB that it might work better than 'still' open air, as still open air is.. well, never actually still. So seeing as open air is always flowing, rather randomly at that, my thought was that it might be better if you can at least cause the flow to mostly go up. Other than that the only other reason would be be for convenience sake really, it would be nice if we could devise a way to work in the open air, though isolating a strong species/strain is likely a better option than simply turning a fan on when it comes to that.


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OfflinePhosCap
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Re: Poor mans flow 'hood' idea. [Re: BloodKil]
    #18794988 - 09/03/13 03:56 PM (10 years, 5 months ago)

Any updates? :strokebeard:


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Offlinekrypto2000
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Re: Poor mans flow 'hood' idea. [Re: PhosCap]
    #18796191 - 09/03/13 08:18 PM (10 years, 5 months ago)

Yeah, I'd been meaning to ask. How'd it turn out?


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Offlinekrypto2000
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Re: Poor mans flow 'hood' idea. [Re: krypto2000]
    #18804184 - 09/05/13 05:05 PM (10 years, 5 months ago)

Maybe he discoverd a new super fast way to grow mushrooms and has been tripping balls for the past week :rofl:.


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OfflinePhosCap
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Re: Poor mans flow 'hood' idea. [Re: krypto2000]
    #18804208 - 09/05/13 05:09 PM (10 years, 5 months ago)

Quote:

krypto2000 said:
Maybe he discoverd a new super fast way to grow mushrooms and has been tripping balls for the past week :rofl:.




Or maybe through the experiment he found a contam that actually consolidated his whole body. oh no :eek:


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Psychoactive Fungi of the World        TLs            Franks teks and Tips        Stonesun's..
Spitballs Quick Rye Prep


Edited by PhosCap (09/05/13 05:10 PM)


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Offlinekrypto2000
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Re: Poor mans flow 'hood' idea. [Re: PhosCap]
    #18804447 - 09/05/13 06:18 PM (10 years, 5 months ago)

An anti-tek :eek:


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InvisibleToe_Jam
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Re: Poor mans flow 'hood' idea. [Re: krypto2000]
    #18804571 - 09/05/13 06:45 PM (10 years, 5 months ago)

Someone needs to sneak into this thread's bedroom and stab the motherfucker in the heart.


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God lay his finger at the Mouth of the Serpent

March 1984


A pleasing land of drowsy head it was,
Of dreams that wave before the half-shut eye,
And of gay castles in the clouds that pass,
For ever flushing round a summer sky. -Castle of Indolence


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OfflinePsilosopherr
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Re: Poor mans flow 'hood' idea. [Re: Toe_Jam]
    #18805167 - 09/05/13 09:06 PM (10 years, 5 months ago)

Quote:

Toe_Jam said:
Someone needs to sneak into this thread's bedroom and stab the motherfucker in the heart.



for real. I'd bet money that the test plates contamed, and am tired of seeing this pop up in "my threads" with new posts :lol:


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InvisibleJuiceh
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Re: Poor mans flow 'hood' idea. [Re: Toe_Jam]
    #18805174 - 09/05/13 09:07 PM (10 years, 5 months ago)

Quote:

Toe_Jam said:
Someone needs to sneak into this thread's bedroom and stab the motherfucker in the heart.




Go!


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Offlinekrypto2000
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Re: Poor mans flow 'hood' idea. [Re: Juiceh]
    #18806841 - 09/06/13 10:14 AM (10 years, 5 months ago)

Maybe he lives in Korea and the ceiling fan decapitated him :rofl:.


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