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retaardvark
Stranger

Registered: 12/22/12
Posts: 64
Last seen: 4 years, 8 months
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First Visible Colonization from LC Six Weeks In; Microscopic Colonization Battle? -UPDATE
#18748630 - 08/23/13 07:31 PM (10 years, 5 months ago) |
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New update
One of the rye bags in the description of my initial problem below begun to show mycelium about eight weeks after being inoculated with a slightly-contaminated LC. I say slightly, because it took so long to begin to see mycelium, but now it looks healthy and vigorous. Busted up the apple-sized chunk, and it chewed through the three to four quarts of rye in four days.


Shiny-bag camera syndrome evident. It looks a robust, bright, healthy white in person, with no spots of uncovered grains, metabolite build-up, or separate, distinct mycelial mass indicative of competitor molds. That red spot is RTV silicone. I know that just because it doesn't look contaminated it isn't; are there any special precautions one should take when spawning a ten-week old rye bag in 1:1 ratio to properly-pasteurized coir/verm/gypsum?
Bonus update: the bag I first described below in my initial thread was certainly contaminated with a pink mold and a spot of bacteria. Was sad to see her go, but she was throwing off +10 degree/ambient temps, so I knew she wasn't long for the world. This bag, one of the two that showed no growth at the initial time of writing, colonized with just a degree or two extra surface temperature in a cooler room. The other bag is still completely devoid of macroscopic or smelly growth. Smells like fresh rye when I push my nose to the filter.
Background
Hey there fellow Shroomerites! Let me be frank (not literally, but Hi Frank! your teks) and say I need no advice; this is more for discussion.
For reasons I now know to be unwise, did .5cc spore solution each to about 10 1-pint DLME LCs, took the best-looking and blindly inoculated four ~four quart rye bags, prepared a-la-RR, but a little on the dry side. Used 25 cc each bag. Soon after making, I knew they'd be bust, so tossed 'em in a corner.
About six weeks in, there was no growth whatsoever, so fearing my rye contained fungicide, I opened one bag for a few seconds to allow mold spores in, then resealed, and that took off. I <obviously> knew this would contaminate if anything grew.
Just now, I go back to look at the remaining three, and am startled to see a huge (~one quart) chunk of mycelium of some kind. I really closely examined as best I could under bright light. It looked pretty normal, white, just a little dry.
SO, (away from my clean[er] rooms) I busted it up, dispersed through bag, and am awaiting results. I am not getting my hopes up it is the mycelium I want, but...
Questions...
Have my bags shown no growth because there is a microscopic colonization battle being waged?
My thoughts are thus: LC was most certainly partially contaminated. But now, eight weeks after inoculation, still two bags of rye are completely devoid of macroscopic growth. Let me reiterate that I used 30 cc of highly nutritive liquid with much growth for each bag, and thereafter nothing has happened.
Have any of you experienced similar?
Will update with progress of bag in a day or two.
Edited by retaardvark (09/05/13 10:09 AM)
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retaardvark
Stranger

Registered: 12/22/12
Posts: 64
Last seen: 4 years, 8 months
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Re: First Visible Colonization from LC Six Weeks In; Microscopic Colonization Battle? [Re: retaardvark]
#18750420 - 08/24/13 07:57 AM (10 years, 5 months ago) |
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Bump 
Will not continue to bump if no discussion.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: First Visible Colonization from LC Six Weeks In; Microscopic Colonization Battle? [Re: retaardvark]
#18750819 - 08/24/13 10:00 AM (10 years, 5 months ago) |
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Quote:
retaardvark said:
About six weeks in, there was no growth whatsoever, so fearing fungicidal rye, I opened one bag for a few seconds to allow mold spores in, then resealed, and that took off.
Have any of you experienced similar?

Your LC is bad, first.
Second, "fungicidal rye" is something I've never heard of.
Third, if you expose any sterile media to open air it is going to contam almost every time.
That growth you've got in those bags? That's mold.
Toss em and start over 
Edited by FrankHorrigan (08/24/13 10:46 AM)
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SpitballJedi
Ancient Astronaut


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: First Visible Colonization from LC Six Weeks In; Microscopic Colonization Battle? [Re: FrankHorrigan]
#18750958 - 08/24/13 10:44 AM (10 years, 5 months ago) |
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Non-organic grain producers sometimes use fungicide.
I only buy organic.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: First Visible Colonization from LC Six Weeks In; Microscopic Colonization Battle? [Re: SpitballJedi]
#18750972 - 08/24/13 10:46 AM (10 years, 5 months ago) |
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Oh, ok, that makes sense.
I only ever use organics for my cultivation so I didn't even think of that.
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SpitballJedi
Ancient Astronaut


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: First Visible Colonization from LC Six Weeks In; Microscopic Colonization Battle? [Re: FrankHorrigan]
#18751013 - 08/24/13 10:58 AM (10 years, 5 months ago) |
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"LSD was first synthesized by Albert Hofmann in 1938 from ergotamine, a chemical derived by Arthur Stoll from ergot, a grain fungus that typically grows on rye."
http://en.wikipedia.org/wiki/Lysergic_acid_diethylamide
Just a neat little related tidbit.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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retaardvark
Stranger

Registered: 12/22/12
Posts: 64
Last seen: 4 years, 8 months
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Re: First Visible Colonization from LC Six Weeks In; Microscopic Colonization Battle? [Re: SpitballJedi]
#18802409 - 09/05/13 07:58 AM (10 years, 4 months ago) |
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Bump with pics. Would appreciate discussion of what went on (microscopic contamination battle?), as well as advice on spawning this bag in 1:1 ratio to properly-pasteurized coir/verm/gypsum. Anything special? Should I bust up the bag a little to see if it recovers? I know that's a good way to determine its cleanliness, but I'm worried about the extra nutrition expended just to heal itself.
I think I will try to drop a couple colonized grains on agar before I hydrate and spawn. I think I'll bust up the spawn before putting in SAB, spear a grain with a flame-sterilized scalpel, and drop in dishes. Good idea? I just want more, and clean, cultures.
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