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incubescence
Meow


Registered: 02/05/13
Posts: 33
Last seen: 9 years, 2 months
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Still Air NotBox
#18746100 - 08/23/13 08:33 AM (10 years, 5 months ago) |
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Hi all! Very first tek here, I'm going to show you how I do all of my still air work without a box.
 This tek uses:
a 20 gallon ziploc bag
one widemouth jar lid band
one big yellow cleaning glove (whichever hand you want to use-if you're righthanded, I recommend the right glove unless you're one of those weird people that's likes to practice being ambidextrous in case your hand ever gets chopped off. It COULD happen...)
one band clamp that will fit over the widemouth lid band, OR do like I do now and buy the 10pk of bands that are about the same size as a regular mouth jar, but unscrew them from themselves and then screw them together to make a bigger one. they ARE reusable and handy as hell. My next tek will show how to use them to make GE ports with tyvek on oven roasting bags so you can make your own spawn bags.
silicone as a sealant/gasket
Take the widemouth band.

Take some diagonal cutters, a pair of nail trimmers, or whatever you can and notch around the top so you can fold the notches down. I use about a 3/4 in. spacing.


Stuff the band into the glove with the lip of the band toward the wrist of the glove and the notched end toward the fingers.

Run a ring of silicone along the inside of the lid band.

Fold the extra down and make it nice.

Open the 20gal ziploc and put it inside, against one of the sides, toward the middle of the bag so you will have room to move your hand.

Flip the 20gal ziploc partly inside out so you remember where the lid band was placed

Goober up the outer ring of the glove/band with some silicone

Put the glove/band back in its spot, flipping the bag right side out again and gather it up

Goober up the bag along the outline of the band with more silicone.

take a popsicle stick and smooth that out.

I like to let it dry a few minutes so there's less tack putting on the band clamp, but eventually that goes on, tightened LIGHTLY.

Let that dry half an hour and then tighten the band clamp up. Cut the ziploc out of the hole of the glove/band CAREFULLY! 

You now have a 20 gallon ziploc baggie with a glove on it.

For me, this is enough room, and reasonable enough protection. When my jars get done PCing and pressure drops, I take my jars out, open this up, stick them in. Put it back into the PC, with some air in the ziploc for a light boil for ~3-5mins. This has worked VERY well for doing my spore solutions.
Total cost of everything in the setup is 15 bucks, and you end up with 2 extra 20 gal ziplocs (I also do this in 10gals so I get 3 extra), an odd glove that you can actually use for another if you pull it inside out, and the majority of a tube of silicone to mess around with your other stuff with. if you spent another 5 bucks or got the ten pack of clamps and an extra pair of gloves, you would have enough for 3 of these on about 20 bucks. And still extra silicone.
Seems expensive? Well, when you're not in sterile conditions, it's really nice to have this because you can keep whatever you were working on in there as an extra layer of protection. To transfer items into the bag after it cools (like spore prints) I wrap them in an alcohol dampened paper towel and slip them in thru the zipper, keeping that area of the bag as flat as possible to keep new air from getting in.
Tell me what you think lol
Edited by incubescence (08/24/13 03:49 PM)
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Oh man.
It seems like a lot of work to fix what is pretty much a non-issue...plus I bet there will be air forced in around the seal of the ziplock when you move around in there with the gloves attached.
If you want to work in still air without a box, just make a makeshift tent out of something and work inside. That's what I do, though I do it with a swanky grow tent 

The box is pictured inside there but I do all my work with filter bag inoculations box-free.
Edited by FrankHorrigan (08/23/13 11:06 PM)
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incubescence
Meow


Registered: 02/05/13
Posts: 33
Last seen: 9 years, 2 months
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Quote:
FrankHorrigan said: Oh man.
It seems like a lot of work to fix what is pretty much a non-issue...plus I bet there will be air forced in around the seal of the ziplock when you move around in there with the gloves attached.
Nope, these are airtight. if you compress them, they will maintain negative air pressure. It isn't really a non-issue, yes I know it's not an ideal glove box BUT I do think it's better than say, some of the inverted boxes on tables that I've seen.
Quote:
If you want to work in still air without a box, just make a makeshift tent out of something and work inside. That's what I do, though I do it with a swanky grow tent 
see pics in original reply
The box is pictured inside there but I do all my work with filter bag inoculations box-free.
Honestly? A makeshift tent would mean I'd need to make it out of a sheet and seal the seams (unless I made a twist tie baggie out of a big sheet of plastic-but that's what this is only it has a zip seal), much more work than the 2 minutes of time this takes. 20 gallons of space is plenty big enough! No sealing of seams needed except to install the glove, and yes I do overkill it. I actually used to do spore syringes in a 2gal bag, through the bag, and I wanted more space and dexterity.
I repeat, this is not a still air box. No, it can't do everything a still air box can. But it can do some cool stuff that a SAB can't. It maintains its own air pocket, which you can maintain all the way thru colonization. Even if you have to open it to slip a print in, you can flatten it so you don't admit more air, and if you wipe the alcohol dampened paper towel around that you wrapped the item in, I'm pretty sure this is near-sterile. Throw the jars in while they're still hot so the heat can help kill germs, to me this is more reassuring than hoping Oust gets everything, although I do use that too. Then gather the whole thing and put it somewhere safe.
Thanks for stopping by, Frank. I've read a ton of your stuff, it's been very helpful!
Also, I've decided I'm not going to try PCing these at any pressure. I looked and it's been tried before with lousy results. lol SO I'm going to keep it to oven bags if I need to PC anything. So don't PC it!!! But it does stand up to a light boil. With hot jars, this should be plenty of protection.
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