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Offlinealabamamushie
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Registered: 01/04/13
Posts: 522
Loc: Alabama
Last seen: 3 years, 4 months
Questions That need Answers
    #18743899 - 08/22/13 07:03 PM (10 years, 5 months ago)

Oh Hi im Back after my recent frustration with spore syringes --> Jar ---> monotub Im Fed up not having the results i want, 100% no contamination in my tubs.

I just got back from the Asian Market Scored 7 25 Gram Packets of Agar for $1.39 each so 175 Grams for under $10 they also had potato starch was unsure if this would work for Potato dextrose so i didnt buy,

:confused:
i know you can't sterilize plastic petris  so do i buy pre-sterile ones
and pour them up in the SAB? and if i was to do this would i mix my PDA with Boiling water so it sterilizes the agar? i dont have the money for glass petris at the moment

or should i start with something as easy as a 1/4 wide mouth jar mix my PDA up pour and pc for 30-35 mins

i still gotta make a trip to home depot to finish assembling my SAB
i just want to be sure of things so i can finish my SAB and JUMP IN AGAR!!!


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InvisibleSuperFly
Still in the Space Race
I'm a teapot User Gallery


Registered: 05/19/13
Posts: 1,032
Loc: Dark side off the moon
Re: Questions That need Answers [Re: alabamamushie]
    #18743933 - 08/22/13 07:11 PM (10 years, 5 months ago)

Boiling water will not sterilize your agar mixture! You will have to PC it for 20-30 mins at 15 psi to achieve that. And yes pour into Petri dish in a SAB while the agar is still hot but not burning your hand off. The jar should still be warm, if you wait too long you will have a big jar of solid PDA


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Offlinealabamamushie
Tmoney
Male

Registered: 01/04/13
Posts: 522
Loc: Alabama
Last seen: 3 years, 4 months
Re: Questions That need Answers [Re: SuperFly]
    #18743966 - 08/22/13 07:17 PM (10 years, 5 months ago)

:foreheadslap: shoulda known to pc agar mixture ina glass jar

gonna try a non pour tek and gonna try to pour my own petris and ill choose whichever suits me the best


Edited by alabamamushie (08/22/13 07:20 PM)


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InvisibleSuperFly
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Registered: 05/19/13
Posts: 1,032
Loc: Dark side off the moon
Re: Questions That need Answers [Re: alabamamushie]
    #18743977 - 08/22/13 07:21 PM (10 years, 5 months ago)

Are you going to use potato flakes or fresh potato?


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InvisibleSuperFly
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Registered: 05/19/13
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Loc: Dark side off the moon
Re: Questions That need Answers [Re: SuperFly]
    #18744021 - 08/22/13 07:33 PM (10 years, 5 months ago)

Ya I don't pour my agar from a master jar. I did one time then realized these cool plastic containers I use are PC able. They are awesome I get 10 for one dollar at my local family owned dollar store. The agar doesn't boil over or nothing, for me at least I've heard of this happening for some people that pre pour their plates.


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Offlinealabamamushie
Tmoney
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Registered: 01/04/13
Posts: 522
Loc: Alabama
Last seen: 3 years, 4 months
Re: Questions That need Answers [Re: SuperFly]
    #18744062 - 08/22/13 07:42 PM (10 years, 5 months ago)

ill be using potato flakes and damn they seem better then petris
and you can pc them you say? ,daum im in ima have to try these pour agar pc in those and inoculate :O


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Offlineblojo02184
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Registered: 05/15/13
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Re: Questions That need Answers [Re: SuperFly]
    #18744074 - 08/22/13 07:44 PM (10 years, 5 months ago)

You can wipe with iso-alcohol or bleach/water, and then pour.

I'm going to do the same thing soon.


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InvisibleSuperFly
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Registered: 05/19/13
Posts: 1,032
Loc: Dark side off the moon
Re: Questions That need Answers [Re: alabamamushie]
    #18744140 - 08/22/13 08:00 PM (10 years, 5 months ago)

Quote:

Roadkill said:
This is how I make my PDYA :

5 grams instant potato's "Betty Crocker Potato Buds"
9 grams of agar agar
7 grams of pure honey
1 gram of nutritional yeast mini flake
500 ml of purified water
 



Here is a recipe from an OG that I use.
Yup they are completely PC able, and i dont have to wrap them with parafilm or glad wrap. What I do is throw them in a bigger tubberware container so I don't individually wrap each one with foil and so they are all not floating around in the pressure cooker. If you do find some make sure they are microwave/dishwasher safe, otherwise they might melt. I'm looking around to see if I can find the same ones I use with the same price. Found a few that are more then what I pay for but still comes with 10.

Only downside I found with these containers is they are not as wide as a normal Petri dish (less serface area) but they work and are cheap.


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Offlinealabamamushie
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Registered: 01/04/13
Posts: 522
Loc: Alabama
Last seen: 3 years, 4 months
Re: Questions That need Answers [Re: SuperFly]
    #18744293 - 08/22/13 08:36 PM (10 years, 5 months ago)

aslong as you can cut wedges from the agar ill be good i just plan to agar wedge to grain jar see if i cant dabble into some isolation and try to find a good fruiting genetic or ill just take a clone from a worthy fruit.

thanks i planned to use that just without the yeast :smile:
lets get this SAB built and do some trial and error!!!
i shall Stack monos to the ceiling!!


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InvisibleSuperFly
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Registered: 05/19/13
Posts: 1,032
Loc: Dark side off the moon
Re: Questions That need Answers [Re: alabamamushie]
    #18744614 - 08/22/13 09:56 PM (10 years, 5 months ago)

Ya you can definately innoc from wedges with these! I've found something similar to the ones I use but it says not recommended for microwave or dish washer. But everywhere else says the are microwave safe. Google Sure fresh mini storage containers and the cheapest place would be the dollar tree we site. These things are expensive on eBay.


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InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Questions That need Answers [Re: alabamamushie]
    #18744799 - 08/22/13 10:39 PM (10 years, 5 months ago)

I'd recommend pouring your own. RR's agar tutorial is completely perfect for this.

I also have a thread about agar going if you'd like to take a look or ask me any questions there :thumbup:

Potato starch works great.

10g agar-agar
10g potato starch
7g dextrose
500mL water

Throw some brewers yeast in there, 1 gram, if you have any laying around.

Here is an amazing recipebook for agar courtesy of aloha medicinals.
Good to see you again, my man.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflineDeadPhan
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Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
Re: Questions That need Answers [Re: SuperFly]
    #18744817 - 08/22/13 10:43 PM (10 years, 5 months ago)

Quote:

TheMrMACdaddy said:
Ya I don't pour my agar from a master jar. I did one time then realized these cool plastic containers I use are PC able. They are awesome I get 10 for one dollar at my local family owned dollar store. The agar doesn't boil over or nothing, for me at least I've heard of this happening for some people that pre pour their plates.




That's awesome those are pc able.  Do they show what they are composed of specifically.  Did you just try this on a whim?  As I know many plastics won't make it in a PC. I would love to find out where to find these.  Pretty much a gold mine if I do. Thanks for the heads up and in advance on any more info!


--------------------
 


Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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InvisibleSuperFly
Still in the Space Race
I'm a teapot User Gallery


Registered: 05/19/13
Posts: 1,032
Loc: Dark side off the moon
Re: Questions That need Answers [Re: DeadPhan]
    #18744914 - 08/22/13 11:13 PM (10 years, 5 months ago)

@ FrankHorrigan ya I know most people would recommend pouring them, but i wasn't to happy trying to pour 10+ dishes in my little SAB(i believe its around a 22 quart tote) I Did a test one day and then never looked back. Just working with what i got. Big fan of your write ups btw! :thumbup:

@DeadPhan the packaging doesn't say what the are composed of, looks like very cheap packaging actually lol. One day just for shits and giggles i decided to wrap one up completely in foil to see if it would survive.. To my surprise it did. I prefer the rectangle ones myself. I did a quick search online for Alabamamushie but couldn't find the same brand, if I do find a source for the same price and same brand I will be sure to let you guys know.


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InvisibleSuperFly
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I'm a teapot User Gallery


Registered: 05/19/13
Posts: 1,032
Loc: Dark side off the moon
Re: Questions That need Answers [Re: DeadPhan]
    #18753484 - 08/24/13 11:02 PM (10 years, 5 months ago)

Well I can't find them online but my store by my house just stocked up and has a bunch of them. If you really want them pm me and we can work something out with shipping and all that.


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