Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineAce1928
Experimenter
Male


Registered: 08/09/13
Posts: 181
Loc: NSW
Last seen: 5 years, 2 months
Stirring LC THROUGH liquid agar
    #18741026 - 08/22/13 06:25 AM (10 years, 6 months ago)

Hey guys,

I haven't tried this yet but I am very curious to see what happens, unless you guys have tried it before.

What would happen if you were to get a clean LC and mix it through your agar in the glovebox after it has been sterilised while it is still liquid (right around the 40C mark) just before it sets solid?
And then getting those agar plates into a fridge or something to cool them down as fast as possible

I think this could potentially make the agar colonise very very fast. But I could be wrong completely.

There are 2 reasons why I believe that the mycelium will recover from the temp
1) In QLD Australia there are cubes growing in open fields everywhere. There are MANY days throughout the summer time where the temperatures far exceed 40C out in the open. The cubes survive just fine
2) Mycelium is really tough stuff. I have seen it recover from some ridiculous stuff

So has anyone tried this?
Anyone theorise what might happen?

Cheers guys

Ace

PS I did use the search engine


--------------------
Only by embracing that which mother nature has supplied to us can we hope to ever peacefully exist on this planet


Extras: Filter Print Post Top
InvisiblePestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
Re: Stirring LC THROUGH liquid agar [Re: Ace1928]
    #18741140 - 08/22/13 07:44 AM (10 years, 6 months ago)

I think that with the right agar recipe, it will perform optimal without the need for addition of LC. There's dozens of different recipes. With malt, dextrose, potato water, cat food, horse shit, you name it. Agar is already a fast colonizer.

:twocents:


--------------------


The Corbett Report
Open Source Intelligence News



Extras: Filter Print Post Top
OfflineAce1928
Experimenter
Male


Registered: 08/09/13
Posts: 181
Loc: NSW
Last seen: 5 years, 2 months
Re: Stirring LC THROUGH liquid agar [Re: Pestile]
    #18741179 - 08/22/13 08:11 AM (10 years, 6 months ago)

I do understand that it is already fast but a potential added benefit would be if you were putting wedges of agar into a grain jar or whatever you want then you would have additional points on and through the agar that would effectively be inoculation points.
If you know what I mean?

It could substantially increase the colonising speed of an agar transfer


--------------------
Only by embracing that which mother nature has supplied to us can we hope to ever peacefully exist on this planet


Extras: Filter Print Post Top
InvisiblePestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
Re: Stirring LC THROUGH liquid agar [Re: Ace1928]
    #18741334 - 08/22/13 09:18 AM (10 years, 6 months ago)

Wait, you want to infuse LC mycelium into sterilized agar?

Interesting, but way out of my league. Better let the TC's handle this one. :smile:


--------------------


The Corbett Report
Open Source Intelligence News



Extras: Filter Print Post Top
InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Stirring LC THROUGH liquid agar [Re: Ace1928]
    #18741921 - 08/22/13 11:33 AM (10 years, 6 months ago)

Quote:

Ace1928 said:
I do understand that it is already fast but a potential added benefit would be if you were putting wedges of agar into a grain jar or whatever you want then you would have additional points on and through the agar that would effectively be inoculation points.
If you know what I mean?

It could substantially increase the colonising speed of an agar transfer




Let me admit that I read this post much more thoroughly than the OP, and now that's out the way.

If speed is your issue than simply inoculate an LC with a wedge from your agar. But then you need to wait for that colonize and blah blah...

If you want to increase the speed of colonization across the board, use a tub-in-tub incubator set at 80F.

All my jars inoc'd with agar wedges get shaken at 5-7 days and are done by 10-14 (at most).  A consistent temp (no dips or jumps) is amazingly beneficial in this regard. I will never use room temp for my jars again.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Extras: Filter Print Post Top
OfflineAce1928
Experimenter
Male


Registered: 08/09/13
Posts: 181
Loc: NSW
Last seen: 5 years, 2 months
Re: Stirring LC THROUGH liquid agar [Re: FrankHorrigan]
    #18743551 - 08/22/13 05:46 PM (10 years, 6 months ago)

Cheers Frank

I will still give this stir through a test but that tub in tub looks awesome. I do have something similar at the moment going on but that looks better across the board haha


--------------------
Only by embracing that which mother nature has supplied to us can we hope to ever peacefully exist on this planet


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* best liquid/agar medium? gray1 2,248 1 05/16/01 11:01 AM
by egghead
* suitable sugars for LC (experiment with pic)
( 1 2 all )
lepiota 6,329 38 06/08/05 12:05 PM
by Hotnuts
* club 99s honey mycelium tek question mytymyc 1,605 9 07/08/05 09:48 PM
by Roadkill
* White stuff above top of LC. Is this contamination?
( 1 2 all )
Blue Helix 5,471 25 06/25/05 09:42 PM
by Holydiver
* I AM IMPRESSED WITH LIQUID CULTURE djsage420 2,320 15 04/02/05 05:28 PM
by agar
* Liquid Growth Medium Question kjb1891 1,518 12 03/21/03 07:36 AM
by dog
* Re: Use agar and break free from spore vendor dependency forever for only $10!
( 1 2 3 all )
Anonymous 7,623 41 02/04/01 12:43 PM
by puscle
* PURE WHITE LIQUID CULTURE PANCAKES
( 1 2 all )
agar 6,390 25 06/01/05 11:22 AM
by agar

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
466 topic views. 31 members, 171 guests and 25 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.019 seconds spending 0.005 seconds on 12 queries.