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BloodKil
Mangler av era mödrar slida


Registered: 03/16/13
Posts: 920
Last seen: 1 month, 15 days
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Re: What to do with 48 glass petri dishes. [Re: FrankHorrigan]
#18737526 - 08/21/13 01:37 PM (10 years, 5 months ago) |
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Quote:
FrankHorrigan said:

This is a great idea for a thread in the pub 
I seriously have a stitch in my side from laughing so hard. Lets watch anne's "ignored by" count climb...

Omfg that would be an epic thread.... lol. Do it.
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: What to do with 48 glass petri dishes. [Re: anne halonium]
#18737540 - 08/21/13 01:41 PM (10 years, 5 months ago) |
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typically, you speak with no experience on the subject.
you yell ignore frank, but , if the thread was reduced to you guys, it would be a boring rehash.
as long as the love letters come in every day, i can get past the usual half dozen hater suspects.

id always prefer ship in a bottle, to shit in a shoe box
i feel no need to start an ignore frank campaign. hes already outdated.
and, alot of peeps know it. its a big topic in the fan mail.
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Edited by anne halonium (08/21/13 01:50 PM)
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: What to do with 48 glass petri dishes. [Re: BloodKil]
#18737805 - 08/21/13 02:29 PM (10 years, 5 months ago) |
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Quote:
BloodKil said:
Quote:
FrankHorrigan said:

This is a great idea for a thread in the pub 
I seriously have a stitch in my side from laughing so hard. Lets watch anne's "ignored by" count climb...

Omfg that would be an epic thread.... lol. Do it.
What exactly would the thread be? Deciding between standard agar pouring tek and annes "ship in a bottle" agar? Or between all of your teks..?
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: What to do with 48 glass petri dishes. [Re: Psilosopherr]
#18737814 - 08/21/13 02:31 PM (10 years, 5 months ago) |
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No, I was thinking "Who wants to jump on the bandwagon? Come ignore the worst troll of them all. Here is a nice link to the ignore anne H page. Feel free to let us know you did it and how it makes you feel."
Look at that, I even know what it will say...
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: What to do with 48 glass petri dishes. [Re: FrankHorrigan]
#18737910 - 08/21/13 02:50 PM (10 years, 5 months ago) |
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running scared frank?

wait till ya find out how these were grown......... give ya a hint.

good luck frank-o-saur chicxulub is coming this fall.
i have to run for yacht club, il let peeps ooogle my pics, while ya all squable, and, add nothing to this thread.
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Edited by anne halonium (08/21/13 02:52 PM)
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BloodKil
Mangler av era mödrar slida


Registered: 03/16/13
Posts: 920
Last seen: 1 month, 15 days
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Re: What to do with 48 glass petri dishes. [Re: Psilosopherr]
#18738136 - 08/21/13 03:29 PM (10 years, 5 months ago) |
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Quote:
rbalzer said:
Quote:
BloodKil said: What exactly would the thread be? Deciding between standard agar pouring tek and annes "ship in a bottle" agar? Or between all of your teks..?

Put the rabble away yo. I was simply chuckling along with franks idea and thought it would be funny as shit if it became a real thread.
As far as your comments to the lack of my teks, I have one done,(in my sig) and am in the midst of writing up my scraping tek for agar inoculation (waiting for a different size scalpel blade to come in as I thought I would test out a #22 vs the standard #11 I currently use... though I might write it up anyway and add pictures later)
I also have my large spawn bag construction tek just waiting till I can find the time to cut up some ez felt and make one up for pics (I am also waiting on a ek punch that's on its way for this to test out as I think it would simplify it even more)
I'm waiting for some oysters to fruit at this very moment before I decide if a whole oat tek is a worth while write up.
And I'm experimenting with blue light...
I could rewrite a bunch of other people's teks as I've seen some do on here, but find that kinda pointless and would rather contribute something unique (as long as they work).. After being in this hobby only 5 months I think I'm doing pretty good at trying to help the community.
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: What to do with 48 glass petri dishes. [Re: BloodKil]
#18738159 - 08/21/13 03:33 PM (10 years, 5 months ago) |
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Quote:
BloodKil said:
Quote:
rbalzer said:
Quote:
BloodKil said: What exactly would the thread be? Deciding between standard agar pouring tek and annes "ship in a bottle" agar? Or between all of your teks..?

Put the rabble away yo. I was simply chuckling along with franks idea and thought it would be funny as shit if it became a real thread.
As far as your comments to the lack of my teks, I have one done,(in my sig) and am in the midst of writing up my scraping tek for agar inoculation (waiting for a different size scalpel blade to come in as I thought I would test out a #22 vs the standard #11 I currently use... though I might write it up anyway and add pictures later)
I also have my large spawn bag construction tek just waiting till I can find the time to cut up some ez felt and make one up for pics (I am also waiting on a ek punch that's on its way for this to test out as I think it would simplify it even more)
I'm waiting for some oysters to fruit at this very moment before I decide if a whole oat tek is a worth while write up.
And I'm experimenting with blue light...
I could rewrite a bunch of other people's teks as I've seen some do on here, but find that kinda pointless and would rather contribute something unique (as long as they work).. After being in this hobby only 5 months I think I'm doing pretty good at trying to help the community. 
Where did you even come from? this is your first post in this thread isn't it?
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Pestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
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Re: What to do with 48 glass petri dishes. [Re: FrankHorrigan]
#18738160 - 08/21/13 03:34 PM (10 years, 5 months ago) |
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Quote:
FrankHorrigan said: No, I was thinking "Who wants to jump on the bandwagon? Come ignore the worst troll of them all. Here is a nice link to the ignore anne H page. Feel free to let us know you did it and how it makes you feel."
Look at that, I even know what it will say...

I did it last night, after all the BS about SAB's and blue light. It suddenly hit me. I've been trolled and so has many others. Just add him to the ignore list. Don't waste your time responding to him.
I feel excellent.
--------------------
   The Corbett Report Open Source Intelligence News
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BloodKil
Mangler av era mödrar slida


Registered: 03/16/13
Posts: 920
Last seen: 1 month, 15 days
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Re: What to do with 48 glass petri dishes. [Re: Pestile]
#18738199 - 08/21/13 03:40 PM (10 years, 5 months ago) |
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Sry might have taken that the wrong way...
You have me quoted above laughing about franks idea... thought you were giving me the angry mob and bitching about my lack of contribution compared to Anne.. (which made me / )
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: What to do with 48 glass petri dishes. [Re: BloodKil]
#18738229 - 08/21/13 03:45 PM (10 years, 5 months ago) |
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Quote:
BloodKil said: Sry might have taken that the wrong way...
You have me quoted above laughing about franks idea... thought you were giving me the angry mob and bitching about my lack of contribution compared to Anne.. (which made me / )
Oh sorry dude. Not what I was going for at all, just talking about anne i believe.
No hard feelings?
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BloodKil
Mangler av era mödrar slida


Registered: 03/16/13
Posts: 920
Last seen: 1 month, 15 days
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Re: What to do with 48 glass petri dishes. [Re: Psilosopherr]
#18738837 - 08/21/13 05:41 PM (10 years, 5 months ago) |
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Quote:
rbalzer said:
Quote:
BloodKil said: Sry might have taken that the wrong way...
You have me quoted above laughing about franks idea... thought you were giving me the angry mob and bitching about my lack of contribution compared to Anne.. (which made me / )
Oh sorry dude. Not what I was going for at all, just talking about anne i believe.
No hard feelings? 
No problem it was my bad for misreading.
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SpitballJedi
Ancient Astronaut


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: What to do with 48 glass petri dishes. [Re: BloodKil]
#18739069 - 08/21/13 06:37 PM (10 years, 5 months ago) |
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seems like a widemouth half pint would be easier than those bottles.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: What to do with 48 glass petri dishes. [Re: SpitballJedi]
#18739104 - 08/21/13 06:49 PM (10 years, 5 months ago) |
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and yet, modern labs love culture bottles. maybe they know something thru experience you dont.

actually, im certain they know things you guys dont.
as usual, ive made my point here. we shall meet again.
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Edited by anne halonium (08/21/13 06:54 PM)
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: What to do with 48 glass petri dishes. [Re: anne halonium]
#18739814 - 08/21/13 09:07 PM (10 years, 5 months ago) |
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Quote:
anne halonium said: and yet, modern labs love culture bottles.
Bullshit. I compound drugs for a living in an ISO 5 clean room. We report to the FDA, the DEA, as well as the EPA. All of these agencies cultures are taken on agar plates and cultured with a soybean casein digest medium (SCDM).
The only bottles that have ever been used in this multi million dollar a year company contain our hypochlorite solution (bleach)
There are growers growing out of bottles so maybe you have gotten confused.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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blojo02184
Big Red



Registered: 05/15/13
Posts: 3,525
Loc: Maine
Last seen: 10 months, 3 days
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Re: What to do with 48 glass petri dishes. [Re: maddchef]
#18739861 - 08/21/13 09:19 PM (10 years, 5 months ago) |
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I can see the use for culture bottles with bacteria, molds, and viruses. Not mushrooms.
I can honestly say after reading all this before bedtime makes me 
Started off thinking someone had too many glass petris... Then some bitch has the bold button stuck says shove it in a bottle...
I'll take it the OP is keeping the petris. Wise decision. Good luck in the future, and if you need them gone let me know. Peace guys
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: What to do with 48 glass petri dishes. [Re: blojo02184]
#18739984 - 08/21/13 09:47 PM (10 years, 5 months ago) |
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yeah hes lucky to have 48 glass ones. Someday
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: What to do with 48 glass petri dishes. [Re: Psilosopherr]
#18740205 - 08/21/13 10:45 PM (10 years, 5 months ago) |
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you guys and your ag labs. i wouldnt waste your time with ag lab 101. ya already have frank. do ya need 2?.
lets break this down. assume one has the culture below.
now, note all the tools in the box. do not be afraid.

now imagine this outrageous idea you have clean culture, and tools, note ,an actual glass syringe one preps a sterile bottle of water, with PC, when cooled, one adds the water, to the cultures, and shakes hard. in seconds , the big ones, make a .5 liter of slurry
now, put on your thinking cap. how many grain containers that does in seconds.

answer is lots , and lots , in minits. pic above alone, can make gallons in seconds. and i get extremely low contam rates.

helps to have real large syringes.
now, we sit down, and you can have your plates and tubes.

i dont do plastic syringes , so i dont have a pic.
bet ya dont learn that at ag vo-tech.
fact is, culture bottles out run traditonal glass ops.
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Edited by anne halonium (08/21/13 10:59 PM)
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: What to do with 48 glass petri dishes. [Re: anne halonium]
#18740279 - 08/21/13 11:13 PM (10 years, 5 months ago) |
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I was meaning to order a glass syringe anyway. They seem cool AND useful for a variety of things.
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Novanity1
Student


Registered: 08/29/10
Posts: 326
Last seen: 9 years, 1 month
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Re: What to do with 48 glass petri dishes. [Re: Psilosopherr]
#18740306 - 08/21/13 11:21 PM (10 years, 5 months ago) |
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I like the idea of getting a totally contam free spore swab from those culture bottles.... however, how on earth would you be able to isolate sectors? If there is sectoring in the back end of the bottle there's no way in hell you'll be able to get your scalpel there and sector properly.
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: What to do with 48 glass petri dishes. [Re: Psilosopherr]
#18740315 - 08/21/13 11:24 PM (10 years, 5 months ago) |
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note the pic above, there is a screw driver for every occasion
k peeps, ill be heading over to the main house on the other island, for my bi summer vacation.
ill only be doing my web blog from that location.
since ive been here, ive only commented posted on threads.
when i return, i will ONLY post on my OWN threads.
ya ,all are a joy and so informative. i promise ill hurry back.
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Edited by anne halonium (08/21/13 11:25 PM)
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