Home | Community | Message Board

MRCA Tyroler Gluckspilze
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Bulk Substrate   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineheadofmike
trich. farmer
 User Gallery


Registered: 08/09/07
Posts: 374
Last seen: 8 years, 6 months
higher contam rate with plastic lids
    #18734190 - 08/20/13 08:37 PM (10 years, 6 months ago)

I made a batch of plastic self sealing lids for my grain spawn. But it seems to have a terribly high contam rate compared to metal lids. Anyone else have a similar result? Or an opinion to offer?


Extras: Filter Print Post Top
InvisibleOeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon Flag
Re: higher contam rate with plastic lids [Re: headofmike]
    #18734240 - 08/20/13 08:47 PM (10 years, 6 months ago)

I'm not sure what lids you have, but I use plastic lids without issues.
Are you sure you don't have a contaminated culture?


--------------------


spread love
love is everything
2013 finds
medicinal psilocybin tincture drops
cannabis pics


Extras: Filter Print Post Top
Offlineheadofmike
trich. farmer
 User Gallery


Registered: 08/09/07
Posts: 374
Last seen: 8 years, 6 months
Re: higher contam rate with plastic lids [Re: Oeric McKenna]
    #18734268 - 08/20/13 08:51 PM (10 years, 6 months ago)

I use ball plastic lids with vacutainer and filter disk. I don't think the culture is contaminated bc at this point I have about 4 different types of lids. Some are the same self sealing but with metal lids. Some are the classic two hole and a filter disk. None of them are contaming. Only my plastic lids. What do you use?


Extras: Filter Print Post Top
InvisibleOeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon Flag
Re: higher contam rate with plastic lids [Re: headofmike]
    #18734299 - 08/20/13 08:57 PM (10 years, 6 months ago)

Same. Ball, with synthetic filter disks, nickel-sized, adhered with blue rtv over 1/4" hole , and a blue rtv injection port. I use the metal ones too, & I like both


--------------------


spread love
love is everything
2013 finds
medicinal psilocybin tincture drops
cannabis pics


Extras: Filter Print Post Top
Invisibletoxetel
׀ǝʇǝxoʇ ●


Registered: 08/13/12
Posts: 708
Re: higher contam rate with plastic lids [Re: headofmike]
    #18734425 - 08/20/13 09:22 PM (10 years, 6 months ago)

I only use Ball plastic lids and they work fine for me. Like you, I use BD Vacutainer stoppers stuck in 1/2" holes, and I use RTV silicone to stick 29mm synthetic filter discs over 1/4" holes.

You say you have four kinds of lids but only got contaminations with the plastic ones -- how many of each did you use? And did you inoculate them all in mixed order? Or were the plastic ones the most recent? I wonder if maybe this is just chance, or if the culture got contaminated after inoculating the metal-lid jars but before the plastic-lid jars.


Extras: Filter Print Post Top
InvisibleOeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon Flag
Re: higher contam rate with plastic lids [Re: toxetel]
    #18734488 - 08/20/13 09:35 PM (10 years, 6 months ago)

There could be other overlooked ideas too that will cause problems for plastic lids.
Sometimes they loosen up during the PC run. Some folks hate these lids for such reasons.
I usually get mine out of the PC when its just a bit warm still, and snug them up.


--------------------


spread love
love is everything
2013 finds
medicinal psilocybin tincture drops
cannabis pics


Extras: Filter Print Post Top
Offlineheadofmike
trich. farmer
 User Gallery


Registered: 08/09/07
Posts: 374
Last seen: 8 years, 6 months
Re: higher contam rate with plastic lids [Re: Oeric McKenna]
    #18736631 - 08/21/13 09:46 AM (10 years, 6 months ago)

Interesting thanks for the input guys. I knocked up 28 jars, about 10 were plastic lids. All from the same lc, completly random outve the pc. All plastic lids have contamed. Im going to do a more isolated run and see how it goes. Cheers


Extras: Filter Print Post Top
Invisibleanne halonium
jaguarette
Female


Registered: 05/07/13
Posts: 1,908
Re: higher contam rate with plastic lids [Re: headofmike]
    #18736682 - 08/21/13 10:01 AM (10 years, 6 months ago)

we use all plastic PP5.
and, run a consistent 95%+ for years.

i see nothing inherent in correct plastic use,
that would cause high contam rates.

i strongly suspect you have other issues,
unrelated to plastic lids.


--------------------
:aliendance:


Extras: Filter Print Post Top
InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: higher contam rate with plastic lids [Re: headofmike]
    #18736691 - 08/21/13 10:03 AM (10 years, 6 months ago)

It's all I ever use:



Check your seals around your filters and make sure to do like Oeric mentioned, tighten them if you remove them from the PC while hot.

I tighten them down, shake to distribute moisture, then loosen one-quarter turn and let cool in a box. The lid will tighten up naturally as it cools.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery

Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: higher contam rate with plastic lids [Re: headofmike]
    #18736696 - 08/21/13 10:04 AM (10 years, 6 months ago)

one of the sucky things about LC is that you can have a contam in part of it and the rest be healthy and you would have no idea.


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Extras: Filter Print Post Top
InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: higher contam rate with plastic lids [Re: SpitballJedi]
    #18736709 - 08/21/13 10:07 AM (10 years, 6 months ago)

Quote:

SpitballJedi said:
one of the sucky things about LC is that you can have a contam in part of it and the rest be healthy and you would have no idea.




Spore syringes this is true for but not for nutrient-laden solutions.

Most times a contam'd LC will fuck everything it touches. It is one way to know your LC is bad.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery

Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: higher contam rate with plastic lids [Re: FrankHorrigan]
    #18736780 - 08/21/13 10:28 AM (10 years, 6 months ago)

Quote:

FrankHorrigan said:
Quote:

SpitballJedi said:
one of the sucky things about LC is that you can have a contam in part of it and the rest be healthy and you would have no idea.




Spore syringes this is true for but not for nutrient-laden solutions.

Most times a contam'd LC will fuck everything it touches. It is one way to know your LC is bad.




Could depend on volume of the LC, how long it's been sitting, how much shaking/stirring, other factors i can't think of.

Cube myc and contams can coexist for a period of time, even if only a short period. That's a fact.

Basically, I agree with you and my thought is not intended to be a definitive solution. Just a possibility to consider if all else fails.

I don't know what order he inoculated his jars, but he may have even pick up a contam at the end.


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Extras: Filter Print Post Top
InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: higher contam rate with plastic lids [Re: SpitballJedi]
    #18736794 - 08/21/13 10:33 AM (10 years, 6 months ago)

Of course they can coexist.

I had an issue with molds infused into my mycelium on agar, running alongside each other, indistinguishable without a microscope. I ended up tossing about 20 isolates that had this issue after many transfers proved futile. The molds would not sporulate until almost precisely 3 days after harvesting the first flush off the bulk substrate.

I had no idea the issue until RR pointed this out to me, I took a scope to it and lo and behold, there it was.

But an LC needs to be shaken in order to break up the mycelium, before it is sucked into the syringe. If it is in the syringe too long, it will need to be shaken before inoculation.

This distributes contams, nutrients, cube myc, and everything else quite evenly.

I don't think it was OP's LC that is the problem, but rather sterile tek, issues with the filters on the lids, etc.

I've never seen a bad LC contam less than 100% of the media inoculated...and I had a few bad ones back in the day :shrug:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery

Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: higher contam rate with plastic lids [Re: FrankHorrigan]
    #18736842 - 08/21/13 10:45 AM (10 years, 6 months ago)

:thumbup:


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Extras: Filter Print Post Top
InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: higher contam rate with plastic lids [Re: SpitballJedi]
    #18736859 - 08/21/13 10:48 AM (10 years, 6 months ago)

I wish I saw you around more often SJ!

All of the regulars from back in the day have mostly taken off :cryariver:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery

Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: higher contam rate with plastic lids [Re: FrankHorrigan]
    #18736900 - 08/21/13 10:56 AM (10 years, 6 months ago)

I hate to say it, but sometimes shroomery sucks. :shrug: There's a few peeps I'm hoping are still gone.

I'm glad you're still kickin it; helpin the noobs. I still pretty much follow your teks.


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Extras: Filter Print Post Top
Invisibleanne halonium
jaguarette
Female


Registered: 05/07/13
Posts: 1,908
Re: higher contam rate with plastic lids [Re: FrankHorrigan]
    #18737149 - 08/21/13 12:02 PM (10 years, 6 months ago)

Quote:

FrankHorrigan said:
I wish I saw you around more often SJ!

All of the regulars from back in the day have mostly taken off :cryariver:





dont cry frank, you have annie now to pick up the slack.:thumbup:



--------------------
:aliendance:


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Bulk Substrate   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* Plastic lid holes, 3/8 or 1/2 inch??
( 1 2 all )
Visigoth 6,267 24 04/12/02 06:02 AM
by cookiewhore
* plastic lids and filter disks-how to use? GaNjAShRooM 3,294 4 03/12/02 03:50 PM
by cookiewhore
* Filter discs and plastic lids si1988crx 2,312 11 03/26/03 09:17 AM
by george castanza
* Plastic lids Daft420 1,774 4 07/24/01 09:01 PM
by Doc
* Easy sterilization w/o plastic lids? Captain_Undeez 1,357 2 04/12/02 10:18 AM
by SixTango
* Re: Source for Plastic Lids? Psychonaut 1,582 5 05/26/00 04:45 PM
by AnubisRonin
* Plastic Lids KitMarlowe 1,077 5 12/30/01 09:53 AM
by Anonymous
* plastic lids??? I_and_I 861 2 07/22/02 06:47 PM
by dracon

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,684 topic views. 15 members, 98 guests and 30 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.005 seconds on 12 queries.