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headofmike
trich. farmer



Registered: 08/09/07
Posts: 374
Last seen: 8 years, 6 months
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higher contam rate with plastic lids
#18734190 - 08/20/13 08:37 PM (10 years, 6 months ago) |
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I made a batch of plastic self sealing lids for my grain spawn. But it seems to have a terribly high contam rate compared to metal lids. Anyone else have a similar result? Or an opinion to offer?
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Oeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon
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Re: higher contam rate with plastic lids [Re: headofmike]
#18734240 - 08/20/13 08:47 PM (10 years, 6 months ago) |
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I'm not sure what lids you have, but I use plastic lids without issues. Are you sure you don't have a contaminated culture?
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headofmike
trich. farmer



Registered: 08/09/07
Posts: 374
Last seen: 8 years, 6 months
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Re: higher contam rate with plastic lids [Re: Oeric McKenna]
#18734268 - 08/20/13 08:51 PM (10 years, 6 months ago) |
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I use ball plastic lids with vacutainer and filter disk. I don't think the culture is contaminated bc at this point I have about 4 different types of lids. Some are the same self sealing but with metal lids. Some are the classic two hole and a filter disk. None of them are contaming. Only my plastic lids. What do you use?
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Oeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon
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Re: higher contam rate with plastic lids [Re: headofmike]
#18734299 - 08/20/13 08:57 PM (10 years, 6 months ago) |
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Same. Ball, with synthetic filter disks, nickel-sized, adhered with blue rtv over 1/4" hole , and a blue rtv injection port. I use the metal ones too, & I like both
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toxetel
׀ǝʇǝxoʇ ●


Registered: 08/13/12
Posts: 708
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Re: higher contam rate with plastic lids [Re: headofmike]
#18734425 - 08/20/13 09:22 PM (10 years, 6 months ago) |
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I only use Ball plastic lids and they work fine for me. Like you, I use BD Vacutainer stoppers stuck in 1/2" holes, and I use RTV silicone to stick 29mm synthetic filter discs over 1/4" holes.
You say you have four kinds of lids but only got contaminations with the plastic ones -- how many of each did you use? And did you inoculate them all in mixed order? Or were the plastic ones the most recent? I wonder if maybe this is just chance, or if the culture got contaminated after inoculating the metal-lid jars but before the plastic-lid jars.
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Oeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon
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Re: higher contam rate with plastic lids [Re: toxetel]
#18734488 - 08/20/13 09:35 PM (10 years, 6 months ago) |
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There could be other overlooked ideas too that will cause problems for plastic lids. Sometimes they loosen up during the PC run. Some folks hate these lids for such reasons. I usually get mine out of the PC when its just a bit warm still, and snug them up.
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headofmike
trich. farmer



Registered: 08/09/07
Posts: 374
Last seen: 8 years, 6 months
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Re: higher contam rate with plastic lids [Re: Oeric McKenna]
#18736631 - 08/21/13 09:46 AM (10 years, 6 months ago) |
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Interesting thanks for the input guys. I knocked up 28 jars, about 10 were plastic lids. All from the same lc, completly random outve the pc. All plastic lids have contamed. Im going to do a more isolated run and see how it goes. Cheers
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: higher contam rate with plastic lids [Re: headofmike]
#18736682 - 08/21/13 10:01 AM (10 years, 6 months ago) |
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we use all plastic PP5. and, run a consistent 95%+ for years.
i see nothing inherent in correct plastic use, that would cause high contam rates.
i strongly suspect you have other issues, unrelated to plastic lids.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: higher contam rate with plastic lids [Re: headofmike]
#18736691 - 08/21/13 10:03 AM (10 years, 6 months ago) |
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It's all I ever use:

Check your seals around your filters and make sure to do like Oeric mentioned, tighten them if you remove them from the PC while hot.
I tighten them down, shake to distribute moisture, then loosen one-quarter turn and let cool in a box. The lid will tighten up naturally as it cools.
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SpitballJedi
Ancient Astronaut


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: higher contam rate with plastic lids [Re: headofmike]
#18736696 - 08/21/13 10:04 AM (10 years, 6 months ago) |
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one of the sucky things about LC is that you can have a contam in part of it and the rest be healthy and you would have no idea.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: higher contam rate with plastic lids [Re: SpitballJedi]
#18736709 - 08/21/13 10:07 AM (10 years, 6 months ago) |
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Quote:
SpitballJedi said: one of the sucky things about LC is that you can have a contam in part of it and the rest be healthy and you would have no idea.
Spore syringes this is true for but not for nutrient-laden solutions.
Most times a contam'd LC will fuck everything it touches. It is one way to know your LC is bad.
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SpitballJedi
Ancient Astronaut


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: higher contam rate with plastic lids [Re: FrankHorrigan]
#18736780 - 08/21/13 10:28 AM (10 years, 6 months ago) |
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Quote:
FrankHorrigan said:
Quote:
SpitballJedi said: one of the sucky things about LC is that you can have a contam in part of it and the rest be healthy and you would have no idea.
Spore syringes this is true for but not for nutrient-laden solutions.
Most times a contam'd LC will fuck everything it touches. It is one way to know your LC is bad.
Could depend on volume of the LC, how long it's been sitting, how much shaking/stirring, other factors i can't think of.
Cube myc and contams can coexist for a period of time, even if only a short period. That's a fact.
Basically, I agree with you and my thought is not intended to be a definitive solution. Just a possibility to consider if all else fails.
I don't know what order he inoculated his jars, but he may have even pick up a contam at the end.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: higher contam rate with plastic lids [Re: SpitballJedi]
#18736794 - 08/21/13 10:33 AM (10 years, 6 months ago) |
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Of course they can coexist.
I had an issue with molds infused into my mycelium on agar, running alongside each other, indistinguishable without a microscope. I ended up tossing about 20 isolates that had this issue after many transfers proved futile. The molds would not sporulate until almost precisely 3 days after harvesting the first flush off the bulk substrate.
I had no idea the issue until RR pointed this out to me, I took a scope to it and lo and behold, there it was.
But an LC needs to be shaken in order to break up the mycelium, before it is sucked into the syringe. If it is in the syringe too long, it will need to be shaken before inoculation.
This distributes contams, nutrients, cube myc, and everything else quite evenly.
I don't think it was OP's LC that is the problem, but rather sterile tek, issues with the filters on the lids, etc.
I've never seen a bad LC contam less than 100% of the media inoculated...and I had a few bad ones back in the day
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SpitballJedi
Ancient Astronaut


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: higher contam rate with plastic lids [Re: FrankHorrigan]
#18736842 - 08/21/13 10:45 AM (10 years, 6 months ago) |
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-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: higher contam rate with plastic lids [Re: SpitballJedi]
#18736859 - 08/21/13 10:48 AM (10 years, 6 months ago) |
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I wish I saw you around more often SJ!
All of the regulars from back in the day have mostly taken off
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SpitballJedi
Ancient Astronaut


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: higher contam rate with plastic lids [Re: FrankHorrigan]
#18736900 - 08/21/13 10:56 AM (10 years, 6 months ago) |
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I hate to say it, but sometimes shroomery sucks. There's a few peeps I'm hoping are still gone.
I'm glad you're still kickin it; helpin the noobs. I still pretty much follow your teks.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: higher contam rate with plastic lids [Re: FrankHorrigan]
#18737149 - 08/21/13 12:02 PM (10 years, 6 months ago) |
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Quote:
FrankHorrigan said: I wish I saw you around more often SJ!
All of the regulars from back in the day have mostly taken off 
dont cry frank, you have annie now to pick up the slack.
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