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OfflineFunkyBuddha
Mycophile


Registered: 08/17/13
Posts: 280
Loc: Australia Flag
Last seen: 8 years, 3 months
Expansion of Liquid Culture
    #18735532 - 08/21/13 02:23 AM (10 years, 6 months ago)

If I were to buy a syringe of liquid culture or create my own initial batch of liquid culture (Specifically for Pleurotus citrinopileatus but I'd be interested in knowing for other Pleurotus species and fungi in general) could I just transfer a relatively small amount into a large jar of the same formula that contains no mycelium and it will colonise the entire jar? So I could effectively have an endless supply of liquid culture? And if so, how would I go about doing it in the most sterile and efficient way?

Peace


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Offlineforrest
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Registered: 11/16/12
Posts: 1,011
Loc: The Netherlands
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Re: Expansion of Liquid Culture [Re: FunkyBuddha]
    #18735663 - 08/21/13 03:20 AM (10 years, 6 months ago)

liquid culture is often not recommended, it's an old discussion.
i think most of the time bacteria survive sterilisation temps or find they're way in trough the filter any way, but if your grains (or other medium) are not too wet, the bacteria can't bloom (because i think they need liquid to move themselves and to have nutrients constantly swirling around them to take up through their membrane. please correct me if i'm wrong)
this explains why you get bacterial contamination most of the time when you prepare grains too wet, even though you sterilise well.

ofcourse since liquid culture is very wet (ha!...) .....


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Edited by forrest (08/21/13 03:22 AM)


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Invisiblewildernessjunkie
Reshitivest
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Registered: 06/13/10
Posts: 8,118
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Trusted Cultivator
Re: Expansion of Liquid Culture [Re: forrest]
    #18735768 - 08/21/13 04:23 AM (10 years, 6 months ago)



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OfflineFunkyBuddha
Mycophile


Registered: 08/17/13
Posts: 280
Loc: Australia Flag
Last seen: 8 years, 3 months
Re: Expansion of Liquid Culture [Re: wildernessjunkie]
    #18735798 - 08/21/13 04:35 AM (10 years, 6 months ago)

Thanks guys, that experiment is quite interesting, I'll try that 1st recipe and compare it with a mycelial slurry recipe I found on mycotopia and do a write up once I have the time. Here's the slurry tek if you haven't seen it already: https://mycotopia.net/forums/holding-tank/58595-making-mycelia-slurry-syringes-bulk-inoculant-tek.html
it looks like an amazing idea and a great way of producing large amounts of inoculating substance from a small amount of spore solution :smile:

Peace


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OfflineOICU812
NC Tree Farm owner
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Registered: 11/06/11
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Re: Expansion of Liquid Culture [Re: FunkyBuddha]
    #18736221 - 08/21/13 07:12 AM (10 years, 6 months ago)



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InvisibleOeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon Flag
Re: Expansion of Liquid Culture [Re: OICU812]
    #18739377 - 08/21/13 07:47 PM (10 years, 6 months ago)

Yes you can expand your culture. Most of the ones you buy on the market are well done and will transfer nicely.
Not endlessly of course, they will run down over time.

A good amount for a half pint jar is a pinch of light dry malt extract powder between two fingertips.
Easy & it works. Its always better to go lean on the sugars, rather than too rich. 

  Punch an injection hole in your jar lid with a nail and if you choose, adhere a filter disk over a hole as well. Put a dab o silicone over your injection hole & when dry:
Mix malt extract & water and PC for about a half hour.

When cool:
Flame the needle of your purchased syringe and when glowing,  inject  into your awaiting jar. 

If done carefully this way, your new innoculant should be as clean as the original.


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