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uninc4life2010
Unincorporated



Registered: 06/05/10
Posts: 1,124
Last seen: 7 months, 12 days
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Better alternatives to LC's?
#18734570 - 08/20/13 09:54 PM (10 years, 5 months ago) |
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I have had a terrible contam storm likely due to bad LC. Basically, 50% contam rate with all my bags, and the other 50% show zero signs of any growth after 3 weeks. I have had fantastic luck with LC's in the past, but that luck seems to have run out. What are some better options for inoculation when using spawn bags?
Here is a couple of options I have heard good things about, that do not involve starting with multispore:
1. Grain LC. This seems like a better option for me. I can visually inspect the colonized grain, and people report greater success over standard LC's.
2. Grain to grain. This seems to be a bit problematic for me, becuase it would require resealing the spawn bags while in the glovebox, and I can't exactly fit my impulse sealer in there. This seems to be a better option for jar users.
3. Agar. I haven't used agar before, but I would be open to it. Again, adding an agar wedge to a spawn bag would require resealing, and I can't really do that inside the glove box. Seems to be a better option for jars.
What do you all suggest?
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DCart
Strangest



Registered: 06/18/13
Posts: 530
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Agar to liquid culture... Make the mycelium contaminate free from agar transfers and then make a liquid culture...
More steps? yep... but you should get a better turn out of uncontaminated bags than spore to LC
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Silence is golden, but duct tape is silver.
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BloodKil
Mangler av era mödrar slida


Registered: 03/16/13
Posts: 920
Last seen: 1 month, 15 days
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Re: Better alternatives to LC's? [Re: DCart]
#18734956 - 08/20/13 11:01 PM (10 years, 5 months ago) |
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Quote:
DCart said: Agar to liquid culture... Make the mycelium contaminate free from agar transfers and then make a liquid culture...
More steps? yep... but you should get a better turn out of uncontaminated bags than spore to LC

Why wo I ld you step backward from the awesomeness of agar Into a lc?
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To the op... #3. AGAR
I'd depends on the size of spawn bags you use.. I agar》master grain jar》8 (full) qts of grain in a bag. If your using smaller sized bags though I would suggest just agar to bag. I have lately been scraping my agar and find it recovers quicker and gives a massive amount of inoculation points by doing so (compared to just a wedge in the jar)
I reseal my bags in the glovebox all the time... and I just got some bag clamps in today so will be trying those out as well. (Should cut down a shit ton of work sealing and cutting) If your gb/sab is too cramped to seal inside (or like me you just don't like to mess with it) you might look at the bag clamp route yourself.
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Re: Better alternatives to LC's? [Re: BloodKil]
#18735095 - 08/20/13 11:34 PM (10 years, 5 months ago) |
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Quote:
BloodKil said:
Quote:
DCart said: Agar to liquid culture... Make the mycelium contaminate free from agar transfers and then make a liquid culture...
More steps? yep... but you should get a better turn out of uncontaminated bags than spore to LC

Why wo I ld you step backward from the awesomeness of agar Into a lc?
Exactly my thoughts.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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FunnyLight
Nom NOm NOM


Registered: 09/12/11
Posts: 1,124
Loc: fuckin Mars man
Last seen: 1 year, 8 months
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Re: Better alternatives to LC's? [Re: mushmagic]
#18735109 - 08/20/13 11:40 PM (10 years, 5 months ago) |
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I still see advantages of LC. You get quick expansion into a lot of media and you're able to avoid opening up spawn bags by sterilizing the substrate with the bag sealed.
-------------------- The most powerful drive through the ascent of man, is pleasure in his own skill. J. Bronowski Home of delicious "Psychedelic Nyotaimori". Thanks Lemmingp for that.
 
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Re: Better alternatives to LC's? [Re: FunnyLight]
#18735135 - 08/20/13 11:47 PM (10 years, 5 months ago) |
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This is true but you still don't know if the LC is clean until you test it out and if it isn't you're back to square one. You can do agar then g2g to speed things up just as fast if not faster. But yes i agree they do have they're advantages too when they're clean.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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OldHam



Registered: 07/19/13
Posts: 1,566
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Re: Better alternatives to LC's? [Re: mushmagic]
#18735300 - 08/21/13 12:44 AM (10 years, 5 months ago) |
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Liquid Culture just seems like a turkey chase to me. Growing bac/mold/whatever for a hobby.
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LemonDude
Scrub


Registered: 05/31/12
Posts: 10
Last seen: 8 years, 5 months
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Re: Better alternatives to LC's? [Re: OldHam]
#18735389 - 08/21/13 01:12 AM (10 years, 5 months ago) |
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I vote agar. even under the most heinous of contaminate likely conditions I've witnessed agar win every time. The added bonus of potential strain saves in the event of a moldy dish is also cool. and you can order pre poured dishes if you are lazy.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 15 hours
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Quote:
uninc4life2010 said: 1. Grain LC. This seems like a better option for me. I can visually inspect the colonized grain, and people report greater success over standard LC's
How can you visually inspect the grains that are not pressed up against the glass?
Grain LCs are no more safer than any other LC.
Quote:
uninc4life2010 said: 2. Grain to grain. This seems to be a bit problematic for me, becuase it would require resealing the spawn bags while in the glovebox, and I can't exactly fit my impulse sealer in there. This seems to be a better option for jar users.
3. Agar. I haven't used agar before, but I would be open to it. Again, adding an agar wedge to a spawn bag would require resealing, and I can't really do that inside the glove box. Seems to be a better option for jars.
What do you all suggest?
Use jars.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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OldHam



Registered: 07/19/13
Posts: 1,566
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Re: Better alternatives to LC's? [Re: PussyFart]
#18735967 - 08/21/13 05:32 AM (10 years, 5 months ago) |
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I think he'd rather hear from a real Trusted Cultivator, not some half cocked newb.
-------------------- The Shallows, Chapter 7, Nicholas Carr
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 15 hours
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Re: Better alternatives to LC's? [Re: OldHam]
#18735986 - 08/21/13 05:37 AM (10 years, 5 months ago) |
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Who are you talking to exactly?
You opted out of the rating system, and posted 650 times in a month....more than half of which are just complete BS nonsense posts....
Pot calling the kettle black?
The best alternative to LC is agar and G2G transfers..... I highly doubt a "real TC" would say any different.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 6 seconds
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Re: Better alternatives to LC's? [Re: OldHam]
#18736043 - 08/21/13 05:59 AM (10 years, 5 months ago) |
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Quote:
OldHam said: I think he'd rather hear from a real Trusted Cultivator, not some half cocked newb. 
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 15 hours
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Re: Better alternatives to LC's? [Re: Stromrider]
#18736046 - 08/21/13 06:01 AM (10 years, 5 months ago) |
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--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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OldHam



Registered: 07/19/13
Posts: 1,566
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Re: Better alternatives to LC's? [Re: Stromrider]
#18736105 - 08/21/13 06:27 AM (10 years, 5 months ago) |
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Just sayin', too many threads are derailed and clogged with amateur opinions. Few of my own, heartfelt, sincere, curious threads were ruint in the same way.
-------------------- The Shallows, Chapter 7, Nicholas Carr
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 15 hours
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Re: Better alternatives to LC's? [Re: OldHam]
#18736110 - 08/21/13 06:30 AM (10 years, 5 months ago) |
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Again you are worried about derailing a thread after the OP's question has already been answered....forgetting about the fact that is was YOU who derailed it in the first place.
You should just stop trying...
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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DCart
Strangest



Registered: 06/18/13
Posts: 530
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Re: Better alternatives to LC's? [Re: PussyFart]
#18736415 - 08/21/13 08:34 AM (10 years, 5 months ago) |
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Hehe, I see my suggestion wasn't really a good one. I just figured if he is dead set on using a syringe to inoculate a bag, then he would have best of luck with growing the strain out in agar, and tryin to prepare a clean liquid culture from that.
Squeezing agar through a syringe seems tough... No?
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Silence is golden, but duct tape is silver.
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Pestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
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Re: Better alternatives to LC's? [Re: DCart]
#18736488 - 08/21/13 09:03 AM (10 years, 5 months ago) |
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If he used a no pour tek and a small jar without filter, he could squirt sterilized water into it and shake.
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   The Corbett Report Open Source Intelligence News
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Quote:
uninc4life2010 said: Here is a couple of options I have heard good things about, that do not involve starting with multispore:
1. Grain LC. This seems like a better option for me. I can visually inspect the colonized grain, and people report greater success over standard LC's.
2. Grain to grain. This seems to be a bit problematic for me, becuase it would require resealing the spawn bags while in the glovebox, and I can't exactly fit my impulse sealer in there. This seems to be a better option for jar users.
3. Agar. I haven't used agar before, but I would be open to it. Again, adding an agar wedge to a spawn bag would require resealing, and I can't really do that inside the glove box. Seems to be a better option for jars.
Oh look, I have a write up just for you 
LC v GLC v Agar
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DCart
Strangest



Registered: 06/18/13
Posts: 530
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Re: Better alternatives to LC's? [Re: Pestile]
#18736571 - 08/21/13 09:25 AM (10 years, 5 months ago) |
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Quote:
Pestilence said: If he used a no pour tek and a small jar without filter, he could squirt sterilized water into it and shake.
Clever! I don't really have much experience growing... Or any. However, I do work with agar. I mainly hunt mushrooms. When I find something neat, I make spore syringes and attempt to decontaminate the cultures using agar. I've been successful with ps. caerulescens(weilii) and a few other species. It's fun stuff.
I'm working on an unidentified gymnopilus at the moment. If it grows out, I'll send the cultures off to people who know what to do from there.
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Silence is golden, but duct tape is silver.
Edited by DCart (08/21/13 09:27 AM)
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SpitballJedi
Ancient Astronaut


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Better alternatives to LC's? [Re: DCart]
#18736732 - 08/21/13 10:15 AM (10 years, 5 months ago) |
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If you absolutely insist on using LC, then agar to LC is your best bet.
I prefer agar to grain. I no longer produce anything on a huge scale. I usually have no more than 3-5 runs going at a time. I keep agar slants and dishes in a mini-fridge. Just food for thought
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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