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Offlineuninc4life2010
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Better alternatives to LC's?
    #18734570 - 08/20/13 09:54 PM (10 years, 5 months ago)

I have had a terrible contam storm likely due to bad LC.  Basically, 50% contam rate with all my bags, and the other 50% show zero signs of any growth after 3 weeks.  I have had fantastic luck with LC's in the past, but that luck seems to have run out.  What are some better options for inoculation when using spawn bags?

Here is a couple of options I have heard good things about, that do not involve starting with multispore:

1. Grain LC.  This seems like a better option for me.  I can visually inspect the colonized grain, and people report greater success over standard LC's.

2. Grain to grain.  This seems to be a bit problematic for me, becuase it would require resealing the spawn bags while in the glovebox, and I can't exactly fit my impulse sealer in there.  This seems to be a better option for jar users.

3. Agar.  I haven't used agar before, but I would be open to it.  Again, adding an agar wedge to a spawn bag would require resealing, and I can't really do that inside the glove box.  Seems to be a better option for jars. 

What do you all suggest?


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InvisibleDCart
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Re: Better alternatives to LC's? [Re: uninc4life2010]
    #18734604 - 08/20/13 10:00 PM (10 years, 5 months ago)

Agar to liquid culture... Make the mycelium contaminate free from agar transfers and then make a liquid culture...

More steps? yep... but you should get a better turn out of uncontaminated bags than spore to LC


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OfflineBloodKil
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Re: Better alternatives to LC's? [Re: DCart]
    #18734956 - 08/20/13 11:01 PM (10 years, 5 months ago)

Quote:

DCart said:
Agar to liquid culture... Make the mycelium contaminate free from agar transfers and then make a liquid culture...

More steps? yep... but you should get a better turn out of uncontaminated bags than spore to LC



:notsureif:

Why wo I ld you step backward from the awesomeness of agar Into a lc?

-------

To the op...  #3.  AGAR

I'd depends on the size of spawn bags you use..  I agar》master grain jar》8 (full) qts of grain in a bag.  If your using smaller sized bags though I would suggest just agar to bag.  I have lately been scraping my agar and find it recovers quicker and gives a massive amount of inoculation points by doing so (compared to just a wedge in the jar)

I reseal my bags in the glovebox all the time...  and I just got some bag clamps in today so will be trying those out as well. (Should cut down a shit ton of work sealing and cutting)  If your gb/sab is too cramped to seal inside (or like me you just don't like to mess with it) you might look at the bag clamp route yourself.


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Invisiblemushmagic
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Re: Better alternatives to LC's? [Re: BloodKil]
    #18735095 - 08/20/13 11:34 PM (10 years, 5 months ago)

Quote:

BloodKil said:
Quote:

DCart said:
Agar to liquid culture... Make the mycelium contaminate free from agar transfers and then make a liquid culture...

More steps? yep... but you should get a better turn out of uncontaminated bags than spore to LC



:notsureif:

Why wo I ld you step backward from the awesomeness of agar Into a lc?




Exactly my thoughts.


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OfflineFunnyLight
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Re: Better alternatives to LC's? [Re: mushmagic]
    #18735109 - 08/20/13 11:40 PM (10 years, 5 months ago)

I still see advantages of LC. You get quick expansion into a lot of media and you're able to avoid opening up spawn bags by sterilizing the substrate with the bag sealed.


--------------------
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Invisiblemushmagic
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Re: Better alternatives to LC's? [Re: FunnyLight]
    #18735135 - 08/20/13 11:47 PM (10 years, 5 months ago)

This is true but you still don't know if the LC is clean until you test it out and if it isn't you're back to square one. You can do agar then g2g to speed things up just as fast if not faster. But yes i agree they do have they're advantages too when they're clean.


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InvisibleOldHam
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Re: Better alternatives to LC's? [Re: mushmagic]
    #18735300 - 08/21/13 12:44 AM (10 years, 5 months ago)

Liquid Culture just seems like a turkey chase to me.
Growing bac/mold/whatever for a hobby.


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OfflineLemonDude
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Re: Better alternatives to LC's? [Re: OldHam]
    #18735389 - 08/21/13 01:12 AM (10 years, 5 months ago)

I vote agar. even under the most heinous of contaminate likely conditions I've witnessed agar win every time. The added bonus of potential strain saves in the event of a moldy dish is also cool. and you can order pre poured dishes if you are lazy.


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OfflinePussyFart
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Re: Better alternatives to LC's? [Re: uninc4life2010]
    #18735830 - 08/21/13 04:49 AM (10 years, 5 months ago)

Quote:

uninc4life2010 said:
1. Grain LC.  This seems like a better option for me.  I can visually inspect the colonized grain, and people report greater success over standard LC's



How can you visually inspect the grains that are not pressed up against the glass?

Grain LCs are no more safer than any other LC.

Quote:

uninc4life2010 said:
2. Grain to grain.  This seems to be a bit problematic for me, becuase it would require resealing the spawn bags while in the glovebox, and I can't exactly fit my impulse sealer in there.  This seems to be a better option for jar users.

3. Agar.  I haven't used agar before, but I would be open to it.  Again, adding an agar wedge to a spawn bag would require resealing, and I can't really do that inside the glove box.  Seems to be a better option for jars. 

What do you all suggest?



Use jars.


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InvisibleOldHam
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Re: Better alternatives to LC's? [Re: PussyFart]
    #18735967 - 08/21/13 05:32 AM (10 years, 5 months ago)

I think he'd rather hear from a real Trusted Cultivator, not some half cocked newb. :rolleyes:


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OfflinePussyFart
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Re: Better alternatives to LC's? [Re: OldHam]
    #18735986 - 08/21/13 05:37 AM (10 years, 5 months ago)

Who are you talking to exactly?

You opted out of the rating system, and posted 650 times in a month....more than half of which are just complete BS nonsense posts....

Pot calling the kettle black?

The best alternative to LC is agar and G2G transfers..... I highly doubt a "real TC" would say any different.


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OnlineStromriderM
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Re: Better alternatives to LC's? [Re: OldHam]
    #18736043 - 08/21/13 05:59 AM (10 years, 5 months ago)

Quote:

OldHam said:
I think he'd rather hear from a real Trusted Cultivator, not some half cocked newb. :rolleyes:




:ban:


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OfflinePussyFart
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Re: Better alternatives to LC's? [Re: Stromrider]
    #18736046 - 08/21/13 06:01 AM (10 years, 5 months ago)

:banbanban:


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InvisibleOldHam
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Re: Better alternatives to LC's? [Re: Stromrider]
    #18736105 - 08/21/13 06:27 AM (10 years, 5 months ago)

Just sayin', too many threads are derailed and clogged with amateur opinions.
Few of my own, heartfelt, sincere, curious threads were ruint in the same way.


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OfflinePussyFart
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Re: Better alternatives to LC's? [Re: OldHam]
    #18736110 - 08/21/13 06:30 AM (10 years, 5 months ago)

Again you are worried about derailing a thread after the OP's question has already been answered....forgetting about the fact that is was YOU who derailed it in the first place.

You should just stop trying...


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InvisibleDCart
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Re: Better alternatives to LC's? [Re: PussyFart]
    #18736415 - 08/21/13 08:34 AM (10 years, 5 months ago)

Hehe, I see my suggestion wasn't really a good one. I just figured if he is dead set on using a syringe to inoculate a bag, then he would have best of luck with growing the strain out in agar, and tryin to prepare a clean liquid culture from that.

Squeezing agar through a syringe seems tough... No? :smile:


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InvisiblePestile

Registered: 05/02/13
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Re: Better alternatives to LC's? [Re: DCart]
    #18736488 - 08/21/13 09:03 AM (10 years, 5 months ago)

If he used a no pour tek and a small jar without filter, he could squirt sterilized water into it and shake.


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InvisibleFrankHorrigan
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Re: Better alternatives to LC's? [Re: uninc4life2010]
    #18736531 - 08/21/13 09:15 AM (10 years, 5 months ago)

Quote:

uninc4life2010 said:
Here is a couple of options I have heard good things about, that do not involve starting with multispore:

1. Grain LC.  This seems like a better option for me.  I can visually inspect the colonized grain, and people report greater success over standard LC's.

2. Grain to grain.  This seems to be a bit problematic for me, becuase it would require resealing the spawn bags while in the glovebox, and I can't exactly fit my impulse sealer in there.  This seems to be a better option for jar users.

3. Agar.  I haven't used agar before, but I would be open to it.  Again, adding an agar wedge to a spawn bag would require resealing, and I can't really do that inside the glove box.  Seems to be a better option for jars. 




Oh look, I have a write up just for you :awesomenod:

LC v GLC v Agar


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InvisibleDCart
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Re: Better alternatives to LC's? [Re: Pestile]
    #18736571 - 08/21/13 09:25 AM (10 years, 5 months ago)

Quote:

Pestilence said:
If he used a no pour tek and a small jar without filter, he could squirt sterilized water into it and shake.





Clever!
I don't really have much experience growing... Or any. However, I do work with agar. I mainly hunt mushrooms. When I find something neat, I make spore syringes and attempt to decontaminate the cultures using agar. I've been successful with ps. caerulescens(weilii) and a few other species. It's fun stuff.

I'm working on an unidentified gymnopilus at the moment. If it grows out, I'll send the cultures off to people who know what to do from there.


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Silence is golden, but duct tape is silver.


Edited by DCart (08/21/13 09:27 AM)


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InvisibleSpitballJedi
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Re: Better alternatives to LC's? [Re: DCart]
    #18736732 - 08/21/13 10:15 AM (10 years, 5 months ago)

If you absolutely insist on using LC, then agar to LC is your best bet.

I prefer agar to grain. I no longer produce anything on a huge scale. I usually have no more than 3-5 runs going at a time. I keep agar slants and dishes in a mini-fridge. Just food for thought


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