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ConcreteWaves



Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 1 year, 2 months
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Agar to Grain Master - now slow speeds
#18734539 - 08/20/13 09:46 PM (10 years, 5 months ago) |
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So I colonized myc on agar plates. Cut out sectors and put onto new agar plates, myc became a tiny bit more aggressive as it should when sectoring to new plates. Then I let them colonize, cut out wedges, and put into fresh grain jars.
They're colonizing at rlly slow speeds.
My myc from spores, once germinated, colonized way faster - i could see a decent amount of growth each day.
I feel like this myc is clinging to life, struggling to grow in a sense.
My house temp is between 75-81/82. I have a feeling where they are colonizing it can get up to 85.
The jars are in the same exact spot i've had jars colonize before from spore syringe, only difference - colonizing in Winter when house was 65-75 and Summer house is 75-85.
Can the room temps create such drastic changes? I mean - it IS growing, and I do know it can sometimes take oddly long. But given I did Agar to agar to grain master into freshly sterilized grains - i was assuming my colonizing times would be way faster than if I had done a spore syringe into fresh grain jar. Any advice?
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FunnyLight
Nom NOm NOM


Registered: 09/12/11
Posts: 1,124
Loc: fuckin Mars man
Last seen: 1 year, 8 months
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Re: Agar to Grain Master - now slow speeds [Re: ConcreteWaves]
#18735026 - 08/20/13 11:15 PM (10 years, 5 months ago) |
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I've noticed this too.
This is my first time going from Agar to Rye grain and I definitely noticed a lag with it. I do believe others have noted this as well and the explanation is something along the lines of the myc transitioning over from consuming simple sugars and food to the rye that is more complex and a bit tougher to break down then the agar media.
This has lead me to the idea of making a "rye puree" as the nutrient base in agar. I'm not sure if others have tried it, or what their tact is for dealing with this stalling though.
I do not believe it is uncommon.
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BloodKil
Mangler av era mödrar slida


Registered: 03/16/13
Posts: 920
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Re: Agar to Grain Master - now slow speeds [Re: FunnyLight]
#18735066 - 08/20/13 11:26 PM (10 years, 5 months ago) |
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IME there is usually a two or three day lag after an agar inoc then they start to explode with growth.
If you transfered away from a ms sample, you may have just got a shit batch of genetics in there... Also another thing I've been trying over my last 3 sets of masters is scraping the mycelium off of the agar and shaking it vigourously (as opposed to cutting a wedge dropping in with agar included and shaking) so far I've found it to not only give an incredible distribution of inoculation points, but it also seems to recover a day or two faster (possibly because it doesn't have the agar to try to recolonize first?)
As far as heat is concerned, that might be a bit on the hot side of things Imo. I beleave I read somewhere on here about starting to see a negative impact past 83°, and the inside of your jars are likely a few degree warmer than the outside temp. (So you may well be getting into that range) Anyway best of luck
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
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Re: Agar to Grain Master - now slow speeds [Re: ConcreteWaves]
#18735156 - 08/20/13 11:55 PM (10 years, 5 months ago) |
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Quote:
myc became a tiny bit more aggressive as it should when sectoring to new plates.
Nah, that's not reasonable. It will change speed and growth habit on different subs. If the new plates were less nutritious it'll grow faster on them searching for nutes.
Quote:
Then I let them colonize, cut out wedges, and put into fresh grain jars.
They're colonizing at rlly slow speeds.
It can just be switching gears for the grain - and/or the grain could be prepared at less than optimal for the myc. Give it a few days and see what happens.
I put some rye flour into my agar mix for just that reason. Never been sure that it makes much difference though. 
PS
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ConcreteWaves



Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 1 year, 2 months
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Re: Agar to Grain Master - now slow speeds [Re: PrimalSoup]
#18736168 - 08/21/13 06:52 AM (10 years, 5 months ago) |
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Quote:
PrimalSoup said:
Quote:
Give it a few days and see what happens.
PS
it's been a week >.> and yea it's colonizing onto the grain - but slowly. Like i said, spore syringe was faster than strong culture on agar.So far.
Ok question: How would I (given im in a regular apartment w/ regular things accessible) find a place to cool down my jars - without slowing the growth of them. But better yet, putting them into optimal colonizing temps. Cuz it's definetly like 85 in my house, it feels like - thermostat says 80-81 but i figure the inside of those jars are probably at 85-87.
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PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 1 year, 5 months
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Re: Agar to Grain Master - now slow speeds [Re: ConcreteWaves]
#18737299 - 08/21/13 12:39 PM (10 years, 5 months ago) |
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The temp isn't a great problem. High temps favor contam growth, but really, it's a continuum, not a life/death measurement. 
PS
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Agar to Grain Master - now slow speeds [Re: PrimalSoup]
#18737395 - 08/21/13 12:59 PM (10 years, 5 months ago) |
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I don't think it is your temps, if anything that speeds growth in the mid 80s. Jars only generate a degree or two temp difference IME, even when fully colonized.
Agar to grain is going to start slower than LC or G2G. This is an unavoidable fact. You have only one major inoculation point if you just toss a wedge in.
I keep my jars at 81F all day and all night. After 7-10 days, I give them a good shake and they finish up in another 3-7 days depending.
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ConcreteWaves



Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 1 year, 2 months
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Re: Agar to Grain Master - now slow speeds [Re: FrankHorrigan]
#18741029 - 08/22/13 06:29 AM (10 years, 5 months ago) |
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Quote:
FrankHorrigan said: I don't think it is your temps, if anything that speeds growth in the mid 80s. Jars only generate a degree or two temp difference IME, even when fully colonized.
Agar to grain is going to start slower than LC or G2G. This is an unavoidable fact. You have only one major inoculation point if you just toss a wedge in.
I keep my jars at 81F all day and all night. After 7-10 days, I give them a good shake and they finish up in another 3-7 days depending.
i put 2-3 wedges in each jar and shook them up for that reason, to give a couple - a few different inoculation points.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Agar to Grain Master - now slow speeds [Re: ConcreteWaves]
#18741711 - 08/22/13 10:39 AM (10 years, 5 months ago) |
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It's still going to be slower than liquid media inoculation, either way. Once you give it a shake at 20% or so, it'll take right off.
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