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Offlinemdove92
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liquid culture question
    #18733556 - 08/20/13 06:31 PM (10 years, 5 months ago)

We made this liquid culture with organic honey and left it in the fridge. the inoculated jars didnt colonize but the left over in the fridge now has pink amd white globs in it, can someone please tell me what they are???


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Offlineyezun
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Re: liquid culture question [Re: mdove92]
    #18733573 - 08/20/13 06:36 PM (10 years, 5 months ago)

I really need some help!!! Ive been trying to figure out how to write my own post but i cant even figure that out anyway Im about to buy some molly, this is my first time buying molly, the guy said its crystallized so the 2.5gs im getting is like a big rock, Its white with a yellow glare now is crystallized molly better than powder or is it better to have the powder than the giant rock of molly lol? can someone please explain to me?


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InvisibleOeric McKenna
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Re: liquid culture question [Re: mdove92]
    #18733698 - 08/20/13 06:59 PM (10 years, 5 months ago)

What species?


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Offlinemdove92
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Re: liquid culture question [Re: Oeric McKenna]
    #18733708 - 08/20/13 07:02 PM (10 years, 5 months ago)

Cambodian. we did lc off of a print, does that make a difference


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OfflineJman420
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Re: liquid culture question [Re: mdove92]
    #18733787 - 08/20/13 07:18 PM (10 years, 5 months ago)

Probably infected. Pink usually is never a good sign. Whoever you got your spore print from probably didn't take much precaution in keeping things sterile.

Btw, weird first choice for spores..Typically you don't put them directly into a LC. I would have started with agar first or made some syringes and shot up some jars.

Best of luck.


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InvisibleTranscendingLife
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Re: liquid culture question [Re: mdove92] * 1
    #18733799 - 08/20/13 07:19 PM (10 years, 5 months ago)

Sounds like molds.
LCs are a waste of time, IME.

Variety of cube wouldn't matter.


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InvisibleOeric McKenna
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Re: liquid culture question [Re: mdove92]
    #18733807 - 08/20/13 07:21 PM (10 years, 5 months ago)

Probably not the best idea. Any minor contaminants will just multiply & grow in a nutrient solution.
  Sterile material is a must.
The reason I asked about species is because Pleurotus djamor will sometimes show pink tones in culture or on grain.

  Probably some various molds.
Best case for a print is agar. If not that, second would be in sterilized water.


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Offlinemdove92
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Re: liquid culture question [Re: Oeric McKenna]
    #18733844 - 08/20/13 07:27 PM (10 years, 5 months ago)

If we were to get another syringe could we make a liquid culture? we just dont want to have to buy a bunch of syringes to do this in bulk


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OfflineJman420
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Re: liquid culture question [Re: mdove92]
    #18733862 - 08/20/13 07:32 PM (10 years, 5 months ago)

I think you are confused on what liquid cultures are...But, best thing to do would be to go buy some sterilized water and and a syringe and make a syringe to shoot up jars..

That is, if you don't have agar..which I take it you don't.

Otherwise you are doomed to fail if you just keep putting your spores directly into a liquid culture jar...Things probably will never turn out well.


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Offlinemdove92
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Re: liquid culture question [Re: Jman420]
    #18733924 - 08/20/13 07:44 PM (10 years, 5 months ago)

I thought making a lc was the process of taking a small amount of spores like 1cc and injecting it into a nutrient solution to make a larger quanity of spore liquid for innoculating. are you saying i can take a syringe and just combine it with sterile water to make a single spore syringe inoculate more jars


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OfflineJman420
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Re: liquid culture question [Re: mdove92]
    #18733943 - 08/20/13 07:49 PM (10 years, 5 months ago)

Quote:

mdove92 said:
I thought making a lc was the process of taking a small amount of spores like 1cc and injecting it into a nutrient solution to make a larger quanity of spore liquid for innoculating. are you saying i can take a syringe and just combine it with sterile water to make a single spore syringe inoculate more jars




You are right to a degree there yes. The idea of a liquid culture is to have "liquid" mycelium so that your jars can skip the spore process of the incubation. You can use spores with liquid cultures if you want to, but more advance growers will use agar to ensure their work is extra sterile and to have an isolated LC strain. Liquid Cultures are often used for bulk,cakes, etc to increase colonization times.

But yea, the basic idea I am suggesting, is to take your spores, mix them with some sterilized water, put the sterilized water with the spores into a syringe, and then use that syringe to shoot up your jars that have your cakes/grains. Make sense?:grin:


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Offlinemdove92
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Re: liquid culture question [Re: Jman420]
    #18734025 - 08/20/13 08:03 PM (10 years, 5 months ago)

So with a 10cc syringe how much can i dilute it and not jepridise the sucess of innoculating with it. also when i make a print and mix it with water how much could i make and if the clumps of spores settle at the bottom will the clearer water still contain sufficient spores for innoculations or do i need to mix it up to have the spores be suspended for drawing a syringe


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Offlinedark3st
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Re: liquid culture question [Re: mdove92]
    #18734079 - 08/20/13 08:12 PM (10 years, 5 months ago)

If you have a clump your using way to much. Try .5cc


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OfflineJman420
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Re: liquid culture question [Re: mdove92]
    #18734093 - 08/20/13 08:16 PM (10 years, 5 months ago)

Phew, making my brain hurt lol.

Ok, so best thing I can suggest is, look up the conversion rates for 10cc to water in cups/tbsp/whatever you are using... and just use the sterilized water (bought at walmart or walgreens or something for 99cents) with your conversion rates, and just mix the spores and water together lol.

Depends on how big the mushroom cap you are using for spore printing is and how many spores its drops...and how much water you will need to accommodate those spores.

Before you inoculate your jars with your syringe, always shake it up to mix up the spores and flame the tip of the needle to sterilize it. If the clumps are not breaking up..then its probably infected.

:goodluck:


--------------------
            --My Everything You Need To Know Tek--

Disregard the haters and "know it alls", it is everything you need to know TO GET STARTED.

If you want to become an expert, you'll need to do a lot more research.
-----------------------------------------------------------------------
                  Open Your Mind To A New Future
-----------------------------------------------------------------------
                            :amanita2::syringe: :mushroom2::prance::mushroom2::cubie::sporedrop:


Edited by Jman420 (08/20/13 08:17 PM)


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Offlinemdove92
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Re: liquid culture question [Re: Jman420]
    #18734186 - 08/20/13 08:36 PM (10 years, 5 months ago)

Thank you all for the help. since my last attempt went poorly making my own innoculant due to me not being as sterile as i should have been i plan on just ordering another syringe of spores can i combined a 10cc syringe from a reputable producer like a sponcer here with 20cc of water to be able to do thirty jars without having to buy three syringes...i would just use a print from last crop but it was first try and it didnt produce much and i ate the ones i didnt use for lc that shouldnt have been tried from my print


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Offlineuninc4life2010
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Re: liquid culture question [Re: mdove92]
    #18734301 - 08/20/13 08:57 PM (10 years, 5 months ago)

Yeah, your LC is contaminated.  That is the problem with LC's, impossible to visually inspect for contams.  A better method would be to use a grain LC.  Just colonize a small jar or WBS with one of those injection port lids, then inject a few hundred cc's of water, shake, then suck out the mycelium water into individual syringes.  This way, you can be very certain that the LC is not contaminated, as you were able to visually confirm that there were no contams present.


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OfflinePussyFart
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Re: liquid culture question [Re: uninc4life2010]
    #18735869 - 08/21/13 05:01 AM (10 years, 5 months ago)

Quote:

uninc4life2010 said:
Yeah, your LC is contaminated.  That is the problem with LC's, impossible to visually inspect for contams.  A better method would be to use a grain LC.  Just colonize a small jar or WBS with one of those injection port lids, then inject a few hundred cc's of water, shake, then suck out the mycelium water into individual syringes.  This way, you can be very certain that the LC is not contaminated, as you were able to visually confirm that there were no contams present.



Really?

How can you visually inspect the grains that are not pressed up against the glass?

Grain LCs are no safer than any other LC...there is no way to inspect every grain.


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InvisibleOeric McKenna
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Re: liquid culture question [Re: PussyFart]
    #18737357 - 08/21/13 12:52 PM (10 years, 5 months ago)

This is true


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