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This_Guy



Registered: 06/27/13
Posts: 45
Loc: Canada
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Question on sterilization of LC mix
#18731685 - 08/20/13 10:54 AM (10 years, 5 months ago) |
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Hi all,
I'm gonna be doing the "Poor man's mycelium syringe tek" and I have a question concerning the sterilization of the mix.
Instead of PCing the mix in a container and then use the syringe, can I just suck the mix in the syringe and PC the syringe ?
I'm just looking to save time here.
Oh and I have everything that I need to do this so I just want a confirmation that it's actually gonna work.
Thanks and sorry for my english.
EDIT: IMO I think this would work. Why not ?
Edited by This_Guy (08/20/13 11:30 AM)
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Question on sterilization of LC mix [Re: This_Guy]
#18733021 - 08/20/13 04:00 PM (10 years, 5 months ago) |
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1) this isn't advanced. 2) it would boil out of the syringe.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Question on sterilization of LC mix [Re: 36fuckin5]
#18736326 - 08/21/13 07:55 AM (10 years, 5 months ago) |
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First off, LC = liquid culture?
Also, a syringe of spores? If so, no -- don't pressure cook them.
Someone asked me this the other day. It's simple.
Get the Pyrex jars w/ septum plugs. Fill with media (LB, TB, or something lame like karo) and pressure cook with lids opened just a crack (so the jars don't explode). When everything cools, open the PC, tighten the lids (hopefully with gloves or just rub down your hands with EtOH if you don't mind dry skin). Then yeah, use an alcohol prep pad and inoculate the culture with a sterile spore syringe via septum plug (one that has not been incinerated; seriously don't PC your spores but I think that must have been a typo).
If you don't let everything cool, negative pressure will draw the plunger down and say bye-bye to everything in the syringe.
Good luck!
EDIT: If LB/TB aren't the best then please correct me. I think that's what I used to use. Karo water won't get you very far though.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
Edited by micro (08/21/13 08:03 AM)
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This_Guy



Registered: 06/27/13
Posts: 45
Loc: Canada
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Re: Question on sterilization of LC mix [Re: micro]
#18736393 - 08/21/13 08:25 AM (10 years, 5 months ago) |
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Yes: LC = Liquid Culture
No: I'm speaking of sterilizing the water and the honey before injecting it into the fully colonized cake. So instead of sterilizing the mix in a jar or anything that can hold it into the PC, and then you suck up the mix in the syringe, I just want to sterilize the syringe with the mix already in it. There is no spores, just water and honey.
I'm talking about this tek: http://www.shroomery.org/67/Poor-Mans-Mycelium-Syringe-Tek
I've been doing it for a while now with good results and I just wanna gain time.
Is this clearer ?
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forrest



Registered: 11/16/12
Posts: 1,011
Loc: The Netherlands
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Re: Question on sterilization of LC mix [Re: This_Guy]
#18737210 - 08/21/13 12:19 PM (10 years, 5 months ago) |
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still like 65fuckin5 said, the water will boil out of the syringe. it will press the plunger out.
-------------------- My Trade List
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This_Guy



Registered: 06/27/13
Posts: 45
Loc: Canada
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Re: Question on sterilization of LC mix [Re: forrest]
#18738342 - 08/21/13 04:05 PM (10 years, 5 months ago) |
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Alright then !
Thanks guys.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
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Re: Question on sterilization of LC mix [Re: This_Guy]
#18738799 - 08/21/13 05:33 PM (10 years, 5 months ago) |
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Don't leave the lids cracked on your jars. They're designed to withstand that kind of pressure, and besides that it should be equal on all sides of the jar.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Question on sterilization of LC mix [Re: 36fuckin5]
#18739020 - 08/21/13 06:23 PM (10 years, 5 months ago) |
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Quote:
36fuckin5 said: Don't leave the lids cracked on your jars. They're designed to withstand that kind of pressure, and besides that it should be equal on all sides of the jar.
Really? They even do that in labs in an autoclave.
Don't unscrew the thing, just give it a small twist so pressure can equalize.
Or do you mean mason jars?
Those probably aren't as solid as pyrex bottles with these.
So yeah, they might not explode.
All my lab experience though has taught me to crack the lids *shrugs*
Of course, nobody was using mason jars either.
Again though, I've never seen a jar explode. So maybe it's a myth???
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
Edited by micro (08/21/13 06:31 PM)
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
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Re: Question on sterilization of LC mix [Re: micro]
#18740878 - 08/22/13 04:20 AM (10 years, 5 months ago) |
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All my lab experience has taught me that if the lid has a filter, the jar will not explode, because the jar will not hold pressure.
All jars in this hobby should have filters, so yea, no need to unscrew anything.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Question on sterilization of LC mix [Re: PussyFart]
#18744975 - 08/22/13 11:31 PM (10 years, 5 months ago) |
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Quote:
Notahacker420 said: All my lab experience has taught me that if the lid has a filter, the jar will not explode, because the jar will not hold pressure.
All jars in this hobby should have filters, so yea, no need to unscrew anything.
Those aren't filters, dude...
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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Noobiesnack
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Re: Question on sterilization of LC mix [Re: micro]
#18772878 - 08/29/13 12:22 PM (10 years, 5 months ago) |
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This poor mans mycelium syringe tek confuses me. What is the point of using electric tape and poking a hole in it then covering it with a coffee filter? couldn't you just cover the hole with micropore tape? Seems like this would be less mess and fuss and less supplies to buy. Correct me if I'm wrong as I still haven't tried LC but I'm trying to figure out how I can do it without my jars exploding.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Question on sterilization of LC mix [Re: Noobiesnack]
#18773935 - 08/29/13 04:26 PM (10 years, 4 months ago) |
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Your jars just won't explode in a PC unless they're flawed. This is what they're designed for. All my LCs get 2 injection ports and nothing else. No filter, PC at 15 PSI for 30 minutes. I've never broken a jar.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
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Re: Question on sterilization of LC mix [Re: 36fuckin5]
#18773967 - 08/29/13 04:33 PM (10 years, 4 months ago) |
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You use one port to equalize the pressure?(filtered needle)?
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (08/29/13 04:34 PM)
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Question on sterilization of LC mix [Re: PussyFart]
#18777353 - 08/30/13 01:33 PM (10 years, 4 months ago) |
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Quote:
Notahacker420 said: You use one port to equalize the pressure?(filtered needle)?
Exactly. Hippie3 style.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Question on sterilization of LC mix [Re: 36fuckin5]
#18779487 - 08/30/13 10:11 PM (10 years, 4 months ago) |
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Quote:
36fuckin5 said:Exactly. Hippie3 style.
LOL, hopefully your heart doesn't seize up from all the pent up asshole anger like his did.
Jars will not explode in a PC if it is run properly. Most people leave the rings loose just in case there is a problem where the pressure inside the jars needs to vent.
Non-liquids, like PF jars, have a greater chance of exploding than liquids. Leave the rings loose for PF and grain jars. With liquids it doesn't really matter.
-FF
-------------------- It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed "If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP) I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
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drake89
Mushroom Magnate



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Re: Question on sterilization of LC mix [Re: fastfred]
#18780910 - 08/31/13 10:58 AM (10 years, 4 months ago) |
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Quote:
fastfred said:
Quote:
36fuckin5 said:Exactly. Hippie3 style.
LOL, hopefully your heart doesn't seize up from all the pent up asshole anger like his did.
Jars will not explode in a PC if it is run properly. Most people leave the rings loose just in case there is a problem where the pressure inside the jars needs to vent.
Non-liquids, like PF jars, have a greater chance of exploding than liquids. Leave the rings loose for PF and grain jars. With liquids it doesn't really matter.
-FF
eh, I've certainly exploded jars of agar before. half gallon ones too, what a mess! But they were tightened before i put them in, so i could shake them. and i forgot to loosen them, so it was totally my fault. Now I use a flask, which feels so much more scientific!
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mastercultivator
Master Cultivator



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Re: Question on sterilization of LC mix [Re: 36fuckin5]
#18781111 - 08/31/13 12:02 PM (10 years, 4 months ago) |
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Quote:
36fuckin5 said: 1) this isn't advanced. 2) it would boil out of the syringe.
--------------------
    I'm awesome sauce with a dash of sunshine.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Question on sterilization of LC mix [Re: fastfred]
#18788531 - 09/02/13 07:03 AM (10 years, 4 months ago) |
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Quote:
36fuckin5 said:
Quote:
Notahacker420 said: You use one port to equalize the pressure?(filtered needle)?
Exactly. Hippie3 style.
Quote:
fastfred said:
Quote:
36fuckin5 said:Exactly. Hippie3 style.
LOL, hopefully your heart doesn't seize up from all the pent up asshole anger like his did.
Jars will not explode in a PC if it is run properly. Most people leave the rings loose just in case there is a problem where the pressure inside the jars needs to vent.
Non-liquids, like PF jars, have a greater chance of exploding than liquids. Leave the rings loose for PF and grain jars. With liquids it doesn't really matter.
-FF
Lol, he banned me from that other site for calling him on BS.
Don't use Mason Jars for LQ. or rather yet, for anything.
Tese are sealed lab grade (Pyrex) jars with rubber septum plugs. You're probably right, they *probably* won't explode but I would still crack the lid slightly. As long as you let everything cool that won't cause contamination (think air coming into a hot jar).
Open the PC once cool and tighten them with gloves.
EDIT: no come to think of it we weren't careful and had a rubber inoculation site pop up and I'm pretty sire we ruined a cooker that way. That's from memory though.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
Edited by micro (09/02/13 07:12 AM)
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Question on sterilization of LC mix [Re: micro]
#18790079 - 09/02/13 02:03 PM (10 years, 4 months ago) |
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Jars are cheap. Those Pyrex flasks are not.
Again, Mason jars are absolutely designed to go in a pressure cooker. On top of that, filtered lids won't allow them to build enough pressure to break. If you break a jar, it probably had a flaw somewhere before pressure cooking.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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RogerRabbit
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Re: Question on sterilization of LC mix (moved) [Re: This_Guy]
#18793262 - 09/03/13 06:47 AM (10 years, 4 months ago) |
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This thread was moved from Advanced Mycology.
Reason: Not an advanced topic, with bad advice being passed around as fact.
Don't listen to nonsense. Lids should always be snugged down tightly. Leaving a lid loose when one is seeking sterility is simply not accepted procedure in mycology. One could make sure he never runs out of gas by simply losing his car keys, but that's not proper procedure either.
Tighten lids all the way, and then use proper pressure cooker or sterilizer procedure. Those who break jars either fail to use a trivet on the bottom or they pop the vent at the end of the cycle to release all the steam at once, breaking the jars. Another problem is people using designer kitchen storage jars as mason jars. Always use Ball or Kerr jars(in the US) for dependable results. RR
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